Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,979,880 C→A 10.2% H225Q (CAC→CAA)  yqeG → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,979,8800CA10.2% 1160.3 / 76.6 347H225Q (CAC→CAA) yqeGputative transporter
Reads supporting (aligned to +/- strand):  ref base C (165/144);  new base A (20/17);  total (186/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 352 total aligned reads displayed.

CAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCG  >  CP009273/2979731‑2980027
                                                                                                                                                     |                                                                                                                                                   
 aaCCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACg                                                                                                                                                    <  2:4143023/150‑1 (MQ=255)
       aTCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATgg                                                                                                                                              <  1:3626464/150‑1 (MQ=255)
       aTCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATgg                                                                                                                                              <  2:1857501/150‑1 (MQ=255)
        tCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGa                                                                                                                                             >  2:3022949/1‑150 (MQ=255)
        tCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATGGa                                                                                                                                             <  1:305832/150‑1 (MQ=255)
         cTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGAt                                                                                                                                            <  2:476885/150‑1 (MQ=255)
          tATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATa                                                                                                                                           <  2:442908/150‑1 (MQ=255)
           aTTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATaa                                                                                                                                          >  2:4195808/1‑150 (MQ=255)
            ttAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATaaa                                                                                                                                         <  2:1785271/150‑1 (MQ=255)
             tAACAACCCAGGTAGAGTTCAATCAGAAGACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAAt                                                                                                                                        <  1:2350485/150‑1 (MQ=255)
              aacaacCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTTGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATg                                                                                                                                       <  2:3387828/150‑1 (MQ=255)
              aacaacCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTTGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATg                                                                                                                                       <  2:3345978/150‑1 (MQ=255)
              aacaacCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATg                                                                                                                                       >  2:2322004/1‑150 (MQ=255)
                caacCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCa                                                                                                                                     <  1:1044774/150‑1 (MQ=255)
                caacCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCa                                                                                                                                     >  1:746174/1‑150 (MQ=255)
                   cccAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTCTGGATAAATGCaaaa                                                                                                                                  >  1:484728/1‑150 (MQ=255)
                   cccAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCaata                                                                                                                                  >  1:3126169/1‑148 (MQ=255)
                       ggTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAAt                                                                                                                              >  1:1432729/1‑150 (MQ=255)
                        gTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCACAAAAAtt                                                                                                                             >  2:4015216/1‑150 (MQ=255)
                          agagTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTAt                                                                                                                           <  1:2019420/150‑1 (MQ=255)
                             gTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGaa                                                                                                                        >  2:3910902/1‑150 (MQ=255)
                               tCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAg                                                                                                                      >  1:4981397/1‑150 (MQ=255)
                               tCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAg                                                                                                                      >  1:5045948/1‑150 (MQ=255)
                                cAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATACATGCAAAAAAATTAGGAAAGt                                                                                                                     >  1:2769792/1‑150 (MQ=255)
                                cAATCAGAATACCCTTCACCAGATATGGATATCGAGTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGt                                                                                                                     <  2:1431444/150‑1 (MQ=255)
                                 aaTCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTc                                                                                                                    >  1:3843828/1‑150 (MQ=255)
                                    cAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCt                                                                                                                 >  2:1877250/1‑150 (MQ=255)
                                     aGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCtt                                                                                                                <  2:4626151/150‑1 (MQ=255)
                                      gAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGACATAGACAGACGTGAAAAATATTGAGAACAAGCTATGGATAATTGCAAAATAAATATGAAATTCGCTTa                                                                                                               >  2:2814023/1‑150 (MQ=255)
                                       aaTACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTAt                                                                                                              >  2:4234759/1‑150 (MQ=255)
                                        aTACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTAtc                                                                                                             >  1:2428993/1‑150 (MQ=255)
                                         tACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTAtct                                                                                                            <  2:1070783/150‑1 (MQ=255)
                                          aCCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTAtctc                                                                                                           <  1:3388924/150‑1 (MQ=255)
                                          aCCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTAtctc                                                                                                           >  2:4903091/1‑150 (MQ=255)
                                           cccTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTAtctaa                                                                                                          >  2:3218985/1‑148 (MQ=255)
                                            ccTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCtcat                                                                                                         >  1:1726082/1‑150 (MQ=255)
                                            ccTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCtcat                                                                                                         >  1:1901466/1‑150 (MQ=255)
                                             cTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCtcatc                                                                                                        <  2:3726544/150‑1 (MQ=255)
                                                 aCCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCt                                                                                                    >  1:1767435/1‑150 (MQ=255)
                                                 aCCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCt                                                                                                    <  1:3759939/150‑1 (MQ=255)
                                                  ccAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTg                                                                                                   >  1:3978064/1‑150 (MQ=255)
                                                  ccAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTg                                                                                                   >  1:2865482/1‑150 (MQ=255)
                                                    aGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTTCCATAGACAGACGTGAAAAATATGGCGAACACTCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCa                                                                                                 >  2:2248877/1‑150 (MQ=255)
                                                    aGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCa                                                                                                 >  2:2616879/1‑150 (MQ=255)
                                                    aGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCa                                                                                                 >  2:2586049/1‑150 (MQ=255)
                                                            atatCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTa                                                                                         >  1:4191757/1‑150 (MQ=255)
                                                            atatCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTa                                                                                         >  2:4857984/1‑150 (MQ=255)
                                                                 gATTCCCATGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGtt                                                                                    >  2:2776991/1‑150 (MQ=255)
                                                                  aTTCCCGTGATGGTTTTCGCCTTTAGCCTTACGCCCTTTATTTCTACGTTTGACATAGACTTTAGTGAAAATTATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTc                                                                                   <  1:3168130/150‑1 (MQ=255)
                                                                      ccGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTg                                                                               >  2:5003643/1‑150 (MQ=255)
                                                                         tGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCt                                                                            <  2:384446/150‑1 (MQ=255)
                                                                             tgTTTTAGACTTTAGCCATACGCCCATTATTTATACGTTTGCCATAGACAGACGTTAAAAATATGGCGAACACGCTATGGATAAATTCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTTTACTGTTCTTTGTCTTTAg                                                                        <  2:3268996/149‑1 (MQ=255)
                                                                                   cGCCTTTAGCCATACGCCCATTATTTCTCCGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGctgcct                                                                  <  2:5196425/150‑1 (MQ=255)
                                                                                   cGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGctgcct                                                                  <  2:841292/150‑1 (MQ=255)
                                                                                   cGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGctgcct                                                                  >  1:22907/1‑150 (MQ=255)
                                                                                    gCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGctgcctg                                                                 >  1:1141360/1‑150 (MQ=255)
                                                                                    gCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGctgcctg                                                                 >  2:2365009/1‑150 (MQ=255)
                                                                                    gCCTTGAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGctgcctg                                                                 <  2:3857134/150‑1 (MQ=255)
                                                                                      cTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAATTGTACTGTTATTTGTCTTTAGCTGCCTGCt                                                               >  2:1006289/1‑150 (MQ=255)
                                                                                               tACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTcc                                                      <  1:4675702/150‑1 (MQ=255)
                                                                                                aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                     <  2:3796796/150‑1 (MQ=255)
                                                                                                aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                     <  2:1178705/150‑1 (MQ=255)
                                                                                                    ccATTATTTCTACGTTTGCCATAGACATACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTNTACNGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCtt                                                 <  1:2667029/150‑1 (MQ=255)
                                                                                                    ccATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCtt                                                 <  1:2125626/150‑1 (MQ=255)
                                                                                                     cATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTc                                                <  1:1628671/150‑1 (MQ=255)
                                                                                                     cATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTc                                                >  1:595934/1‑150 (MQ=255)
                                                                                                        tattACTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTAGGAAAGTCGCTAATCTCATCATCAGCCGACGTGTACTGTTCTGTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGta                                             >  2:2499811/1‑150 (MQ=255)
                                                                                                                cGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAg                                     <  2:4534082/150‑1 (MQ=255)
                                                                                                                 gTTTGCCATAGACAGACGTGAAAAATAT‑GCCAACACGCAAATGAAAAATGCCAAAAAAATATGAAAGTAGGTTTAGTCATAAAAAGCATAAGTGTACAGTTAATTTTATTTAGCGGCCAGCATTAGATTCCAACTTCGTATATTgtatca                                   >  2:5245154/1‑145 (MQ=255)
                                                                                                                  tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgct                                   <  1:224986/150‑1 (MQ=255)
                                                                                                                  tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgct                                   <  1:2741183/150‑1 (MQ=255)
                                                                                                                  tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgct                                   <  2:746174/150‑1 (MQ=255)
                                                                                                                   ttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgctg                                  <  2:4373497/150‑1 (MQ=255)
                                                                                                                    tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgctgc                                 >  1:1034494/1‑150 (MQ=255)
                                                                                                                     gCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgctgct                                <  1:2030158/150‑1 (MQ=255)
                                                                                                                         tAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaag                            <  1:3325118/150‑1 (MQ=255)
                                                                                                                         tAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGAACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaag                            >  2:4712357/1‑150 (MQ=255)
                                                                                                                          agacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaaga                           >  1:66511/1‑150 (MQ=255)
                                                                                                                           gacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaagaa                          <  2:2145790/150‑1 (MQ=255)
                                                                                                                           gacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaagaa                          <  2:2043609/150‑1 (MQ=255)
                                                                                                                            acagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaagaag                         >  1:1180759/1‑150 (MQ=255)
                                                                                                                            acagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCAACCTTCGTATATTGAAGCTGCTAaagaag                         >  2:3945674/1‑150 (MQ=255)
                                                                                                                             cagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGATAaagaaga                        >  2:2710335/1‑150 (MQ=255)
                                                                                                                              agacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAaagaagaa                       <  2:156795/150‑1 (MQ=255)
                                                                                                                                 cGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAggg                    <  1:72272/150‑1 (MQ=255)
                                                                                                                                 cGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAggg                    >  1:884261/1‑150 (MQ=255)
                                                                                                                                 cGTGAAAAATATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAggg                    >  1:3510752/1‑150 (MQ=255)
                                                                                                                                  gTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAgggg                   >  2:2860913/1‑150 (MQ=255)
                                                                                                                                     aaaaaTATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCa                >  1:4873863/1‑150 (MQ=255)
                                                                                                                                      aaaaTATGGCGAACACGCAATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATATGCATAAGTGTACTGTTCTTTGTCTTTAGCAGCCTGCATTCTATTCAACATTCGTATATTGAAGCTGCTAAAGAAGAAGGTGTCAc               >  1:4487827/1‑150 (MQ=255)
                                                                                                                                       aaaTATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCAcc              >  2:1934778/1‑150 (MQ=255)
                                                                                                                                       aaaTATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCAcc              <  1:1323771/150‑1 (MQ=255)
                                                                                                                                        aaTATGGCGAACAAGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCa             <  2:4951006/150‑1 (MQ=255)
                                                                                                                                          tatGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCAtt           <  1:1277698/150‑1 (MQ=255)
                                                                                                                                          tatGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCAtt           <  1:1398849/150‑1 (MQ=255)
                                                                                                                                          tatGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCAtt           >  2:1956889/1‑150 (MQ=255)
                                                                                                                                           atGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCAttt          <  2:2190586/150‑1 (MQ=255)
                                                                                                                                             ggCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTa        >  1:1561088/1‑150 (MQ=255)
                                                                                                                                             ggCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTa        >  2:730350/1‑150 (MQ=255)
                                                                                                                                              gCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTAt       >  2:2483154/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCG  >  CP009273/2979731‑2980027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑