BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,982,873	G→T	9.9%	G71* (GGA→TGA)	yqeJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,982,873	0	G	T	9.9%	1088.8 / 69.0	329	G71* (GGA→TGA)	yqeJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (154/139); new base T (6/29); total (161/168)
Fisher's exact test for biased strand distribution p-value = 5.81e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

CACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCA  >  CP009273/2982725‑2983022

cACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTgg                                                                                                                                                      <  2:4384870/150‑1 (MQ=255)

 aCTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGa                                                                                                                                                     >  1:3561320/1‑150 (MQ=255)

   tGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGTACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAAc                                                                                                                                                   <  2:1851521/150‑1 (MQ=255)

   tGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAAc                                                                                                                                                   >  1:505458/1‑150 (MQ=255)

      cTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTg                                                                                                                                                >  1:5184918/1‑150 (MQ=255)

      cTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTg                                                                                                                                                <  1:2665068/150‑1 (MQ=255)

         ttCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGca                                                                                                                                             <  1:3286653/150‑1 (MQ=255)

          tCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGcaa                                                                                                                                            <  1:4710965/150‑1 (MQ=255)

          tCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGAGTTGAGTGGAACCTGGcaa                                                                                                                                            >  2:3220049/1‑150 (MQ=255)

          tCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATAGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAAAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGcaa                                                                                                                                            >  2:556173/1‑150 (MQ=255)

            ttACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGcaaca                                                                                                                                          <  2:3959762/150‑1 (MQ=255)

                   gCAGAAAAAATGATATCTAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAAc                                                                                                                                   <  1:860634/150‑1 (MQ=255)

                      gAAAAAATGATATATAACGAAACATGCACTGCAAATTTGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCTACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGATTGGAAACAGGCAACAAGGGAAccta                                                                                                                                >  2:4218754/1‑147 (MQ=255)

                          aaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGaaa                                                                                                                            <  1:3915425/150‑1 (MQ=255)

                          aaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGaaa                                                                                                                            >  1:765311/1‑150 (MQ=255)

                              gATATAGAACTATACATGCACTTCATATTGGGTAATTTTTATTGACCAGGGACGAGCAAATCTTACCATCGTCTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTaa                                                                                                                        <  1:4005030/150‑1 (MQ=255)

                                tatataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAAt                                                                                                                      >  2:1666105/1‑150 (MQ=255)

                                tatataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAAt                                                                                                                      <  2:2148013/150‑1 (MQ=255)

                                tatataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAAt                                                                                                                      >  2:4770840/1‑150 (MQ=255)

                                 atataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATc                                                                                                                     <  1:1401997/150‑1 (MQ=255)

                                     aaCGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATa                                                                                                                 <  1:1299652/150‑1 (MQ=255)

                                      aCGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATAc                                                                                                                <  1:1625501/150‑1 (MQ=255)

                                        gAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACgg                                                                                                              >  1:2248019/1‑150 (MQ=255)

                                          aaCATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCg                                                                                                            <  2:86760/150‑1 (MQ=255)

                                           aCATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGa                                                                                                           >  2:3068213/1‑150 (MQ=255)

                                            cATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaa                                                                                                          >  1:1968621/1‑150 (MQ=255)

                                            cATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaa                                                                                                          >  2:2530014/1‑150 (MQ=255)

                                             aTGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaaa                                                                                                         >  1:4378683/1‑150 (MQ=255)

                                               gctcTGCAAATACGTTAATTTTTACTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAACACAATAACACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAata                                                                                                       <  2:3316579/147‑1 (MQ=255)

                                                 aCTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATAtt                                                                                                     >  2:5003481/1‑150 (MQ=255)

                                                  cagCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGGTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTg                                                                                                    <  2:2740760/148‑1 (MQ=255)

                                                     cAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTAGAACAAAAAAATTAAAACAGGAACTGTGGCGTTGGGTGGCACCTGGCAACAAGGTACCAGCGCAAGTAACTCATTGCGGCGAACTATTGAAt                                                                                                 >  2:4890079/1‑150 (MQ=255)

                                                      aaaTTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATTTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATa                                                                                                <  1:1199032/150‑1 (MQ=255)

                                                      aaaTTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATa                                                                                                >  1:3851885/1‑150 (MQ=255)

                                                        aTTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATaca                                                                                              <  1:4609901/150‑1 (MQ=255)

                                                          tGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATAcaca                                                                                            <  1:811028/150‑1 (MQ=255)

                                                           tggTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAATAAAACATGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATg                                                                                          <  1:5020364/149‑1 (MQ=255)

                                                            gttAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATg                                                                                          <  2:3491422/148‑1 (MQ=255)

                                                            ggTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATg                                                                                          >  1:698121/1‑150 (MQ=255)

                                                             gTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATgg                                                                                         >  2:2230251/1‑150 (MQ=255)

                                                              tAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGa                                                                                        >  1:4650383/1‑150 (MQ=255)

                                                               aaTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAt                                                                                       <  1:315770/150‑1 (MQ=255)

                                                               aaTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAt                                                                                       <  1:2043690/150‑1 (MQ=255)

                                                                aTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCTTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAtt                                                                                      >  2:403034/1‑150 (MQ=255)

                                                                aTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAtt                                                                                      <  2:2050794/150‑1 (MQ=255)

                                                                aTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAtt                                                                                      <  2:915775/150‑1 (MQ=255)

                                                                aTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAtt                                                                                      >  2:1307006/1‑150 (MQ=255)

                                                                aTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAtt                                                                                      <  2:1359712/150‑1 (MQ=255)

                                                                    ttAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGaaa                                                                                  <  2:1540276/150‑1 (MQ=255)

                                                                           cctgggACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGa                                                                           <  2:1946886/147‑1 (MQ=255)

                                                                             aGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGaca                                                                         >  2:2400121/1‑150 (MQ=255)

                                                                               ggACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGAcaca                                                                       >  2:2319683/1‑150 (MQ=255)

                                                                                  cGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCGACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGcc                                                                    >  2:2868278/1‑150 (MQ=255)

                                                                                  cGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGcc                                                                    >  1:4259081/1‑150 (MQ=255)

                                                                                    aGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCa                                                                  >  2:3256425/1‑150 (MQ=255)

                                                                                    aGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGAAACAGCCCa                                                                  >  2:5231549/1‑150 (MQ=255)

                                                                                      cAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCAtt                                                                <  1:4809853/150‑1 (MQ=255)

                                                                                      cAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCAtt                                                                <  2:3700647/150‑1 (MQ=255)

                                                                                       aaaTCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCAttt                                                               <  1:2935501/150‑1 (MQ=255)

                                                                                        aaTCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTa                                                              >  2:4271946/1‑150 (MQ=255)

                                                                                          tCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAAc                                                            <  2:1149795/150‑1 (MQ=255)

                                                                                          tCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAAc                                                            <  1:3735418/150‑1 (MQ=255)

                                                                                          tCTTACCATAGACTTTATGTATAACAAAAAAATTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGATAAAGTAAATCATTACGGCGAAATATTGAATACACATTGATTGAAAACTATGACACAGCCCATTTAAc                                                            <  2:4381249/150‑1 (MQ=255)

                                                                                            ttACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACAt                                                          <  1:2691708/150‑1 (MQ=255)

                                                                                            ttACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACAt                                                          <  1:34239/150‑1 (MQ=255)

                                                                                              aCCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCa                                                        >  2:2511966/1‑150 (MQ=255)

                                                                                              aCCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCa                                                        <  1:1511084/150‑1 (MQ=255)

                                                                                                cATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaa                                                      >  2:3407904/1‑150 (MQ=255)

                                                                                                cATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaa                                                      <  1:2779016/150‑1 (MQ=255)

                                                                                                      cTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGt                                                <  2:3831234/150‑1 (MQ=255)

                                                                                                      cTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGt                                                <  2:4721457/150‑1 (MQ=255)

                                                                                                        ttATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTta                                              >  2:1784267/1‑150 (MQ=255)

                                                                                                          aTGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAGTACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaat                                            <  2:5164108/150‑1 (MQ=255)

                                                                                                            gTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaataa                                          >  1:961445/1‑150 (MQ=255)

                                                                                                              ataACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGt                                        >  2:2097737/1‑150 (MQ=255)

                                                                                                                aaCAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGttt                                      >  2:1430529/1‑150 (MQ=255)

                                                                                                                 aCAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATTACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTg                                     >  1:2621962/1‑150 (MQ=255)

                                                                                                                   aaaaaaaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTgag                                   >  2:1138368/1‑150 (MQ=255)

                                                                                                                     aaaaaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGAt                                 >  2:732107/1‑150 (MQ=255)

                                                                                                                      aaaaaTAAAACAGGAACTGTTGCGTTGAGTTGAAACTGGCTACAAGGTACCAGAGAAAGTAAATCAATACGGCGTAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCACAGAAAGTTAATAAGTTTGAGAtt                                <  2:4909996/150‑1 (MQ=255)

                                                                                                                       aaaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTa                               <  1:323471/150‑1 (MQ=255)

                                                                                                                        aaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTAt                              <  2:2507494/150‑1 (MQ=255)

                                                                                                                        aaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTAt                              >  2:1197111/1‑150 (MQ=255)

                                                                                                                          aTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATgg                            >  2:93543/1‑150 (MQ=255)

                                                                                                                           tAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTAAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTCATAAGTTTGAGATTATGGa                           >  1:2447356/1‑150 (MQ=255)

                                                                                                                              aaCAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCa                        >  1:2487431/1‑150 (MQ=255)

                                                                                                                              aaCAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCa                        >  2:2894180/1‑150 (MQ=255)

                                                                                                                               aCAGGAAATGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATAGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCaa                       <  2:870528/150‑1 (MQ=255)

                                                                                                                                 aGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGt                     <  1:1081933/150‑1 (MQ=255)

                                                                                                                                   gAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGTATCAAGTtg                   >  2:679052/1‑150 (MQ=255)

                                                                                                                                    aaCTGTTGCGTTGAGTGGAACCTGGAAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATTGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtga                  <  2:3761685/150‑1 (MQ=255)

                                                                                                                                       tGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatga               >  2:4511634/1‑150 (MQ=255)

                                                                                                                                          tGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgttga            >  2:2461996/1‑150 (MQ=255)

                                                                                                                                          tGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgatga            >  2:4920343/1‑150 (MQ=255)

                                                                                                                                             gTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAg         >  2:1891733/1‑150 (MQ=255)

                                                                                                                                               tGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGAc       <  1:17156/150‑1 (MQ=255)

                                                                                                                                                gAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACt      >  1:3017677/1‑150 (MQ=255)

                                                                                                                                                gAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACt      >  1:380146/1‑150 (MQ=255)

                                                                                                                                                 aGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACtt     <  1:350954/150‑1 (MQ=255)

                                                                                                                                                   tGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGc   >  1:1927034/1‑150 (MQ=255)

CACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCA  >  CP009273/2982725‑2983022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`