Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,774 C→A 10.9% pseudogene (346/447 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,7740CA10.9% 1054.4 / 85.3 327pseudogene (346/447 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base C (141/150);  new base A (14/22);  total (155/172)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

Only 100 of 331 total aligned reads displayed.

AATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATA  >  CP009273/2988625‑2988923
                                                                                                                                                     |                                                                                                                                                     
aaTATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTc                                                                                                                                                       >  1:60836/1‑150 (MQ=255)
        aaTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCt                                                                                                                                               <  2:3864998/150‑1 (MQ=255)
          ttAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTg                                                                                                                                             <  2:4342571/150‑1 (MQ=255)
          ttAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTg                                                                                                                                             >  1:2707798/1‑150 (MQ=255)
              aTTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAg                                                                                                                                         >  1:2936467/1‑150 (MQ=255)
              aTTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAg                                                                                                                                         <  1:5129301/150‑1 (MQ=255)
               ttATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAgg                                                                                                                                        <  2:1760057/150‑1 (MQ=255)
               ttATAGTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgg                                                                                                                                        <  1:2823107/150‑1 (MQ=255)
                tataTTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAggg                                                                                                                                       <  2:189588/150‑1 (MQ=255)
                 atatTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgggg                                                                                                                                      <  2:2409348/150‑1 (MQ=255)
                 atatTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgggg                                                                                                                                      <  2:1868211/150‑1 (MQ=255)
                    ttCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGgaga                                                                                                                                   <  1:4084065/150‑1 (MQ=255)
                      cAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGaaa                                                                                                                                 <  1:4304710/150‑1 (MQ=255)
                      cAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTAGCAGATTTCGTTAACCTTTTCAGGGGAGaaa                                                                                                                                 >  2:4882771/1‑150 (MQ=255)
                       aaaTAATTAAACTTTAGCCATGGATCTATAAGTCATCATGGCTTGCCAAAACATAATTATTCATATCAAAAGAATGTGTTTCAAACATTTATTCAACTAAATAATGTCTTGCTTGTTCGCATATTTCGTTAACATTGTAAGGGGAGAAAc                                                                                                                                >  2:298936/1‑150 (MQ=255)
                        aaTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACa                                                                                                                               <  1:2866319/150‑1 (MQ=255)
                           cATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATg                                                                                                                            >  2:1384225/1‑150 (MQ=255)
                           cATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATg                                                                                                                            >  2:3996044/1‑150 (MQ=255)
                            aTTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGa                                                                                                                           <  1:3939662/150‑1 (MQ=255)
                              tAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGatt                                                                                                                         >  2:817488/1‑150 (MQ=255)
                               aaaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCAGATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGatta                                                                                                                        <  1:3514195/150‑1 (MQ=255)
                                aaCTTGAGCCAGTGAGCTATAAGTCCTCAGGGCTTGCCAACACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                       <  2:2742632/150‑1 (MQ=255)
                                aaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                       <  1:1944202/150‑1 (MQ=255)
                                aaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                       <  1:2382280/150‑1 (MQ=255)
                                aaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                       <  1:4975292/150‑1 (MQ=255)
                                 aCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattatt                                                                                                                      >  1:1108801/1‑150 (MQ=255)
                                    tGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTattaat                                                                                                                   <  2:809060/150‑1 (MQ=255)
                                     gAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTattaatt                                                                                                                  <  2:1015631/150‑1 (MQ=255)
                                       gCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTAttaattaa                                                                                                                <  1:682114/150‑1 (MQ=255)
                                         cAGGGAGCTATCAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAat                                                                                                              <  2:5021929/150‑1 (MQ=255)
                                         cAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAat                                                                                                              <  1:614459/150‑1 (MQ=255)
                                         cAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACAATTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAat                                                                                                              <  2:441470/150‑1 (MQ=255)
                                          aGGGAGCTATCAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                             <  1:2769423/150‑1 (MQ=255)
                                          aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACATAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                             <  2:2308763/150‑1 (MQ=255)
                                          aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                             >  1:3649450/1‑150 (MQ=255)
                                          aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                             >  1:1030454/1‑150 (MQ=255)
                                           gggAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAatat                                                                                                            <  1:185419/150‑1 (MQ=255)
                                            ggAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATAtt                                                                                                           >  1:1407171/1‑150 (MQ=255)
                                             gAGCTATAAGTCCTCAGGGCTTTCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTg                                                                                                          <  2:1250337/150‑1 (MQ=255)
                                              aGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGc                                                                                                         <  1:2300928/150‑1 (MQ=255)
                                               gCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACATTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCt                                                                                                        >  2:5138691/1‑150 (MQ=255)
                                                 tataAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTca                                                                                                      <  2:2044943/150‑1 (MQ=255)
                                                  ataAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTcac                                                                                                     >  1:1592893/1‑150 (MQ=255)
                                                      gTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAAtt                                                                                                 >  2:1304944/1‑150 (MQ=255)
                                                       tCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTa                                                                                                >  1:4944250/1‑150 (MQ=255)
                                                        ccTCAGGGCTTGCCAAAACATAATTATTCATATCCAAAGAATGTGTTTAAAACATTTTTTCAACTAAATAATGTCTGGATTGTTCTCAGATTTCGTTAAACTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTAt                                                                                               <  2:4768520/150‑1 (MQ=255)
                                                         cTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATc                                                                                              <  2:1158146/150‑1 (MQ=255)
                                                          tCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATcc                                                                                             <  1:3318607/150‑1 (MQ=255)
                                                             gggCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCcaca                                                                                          <  2:4108814/150‑1 (MQ=255)
                                                             gggCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCaaca                                                                                          >  2:868738/1‑150 (MQ=255)
                                                                cTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAAt                                                                                       >  2:3329546/1‑150 (MQ=255)
                                                                    ccAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCt                                                                                   >  1:4664602/1‑150 (MQ=255)
                                                                     cAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTg                                                                                  <  2:4460714/150‑1 (MQ=255)
                                                                      aaaaCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGTCTTGTTCGCAGATTTCGTTAACCTTTTCAGGGGAGAAACAATGATTATTAATTAAATATTTCTCACAATTATACACAAATTCATGc                                                                                 >  1:3083302/1‑150 (MQ=255)
                                                                      aaaaCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTATTTAAATATTGCTCACAATTATCCACAAATTCCTGc                                                                                 >  1:4814278/1‑150 (MQ=255)
                                                                      aaaaCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGc                                                                                 <  1:2532073/150‑1 (MQ=255)
                                                                              attattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTaaa                                                                         >  2:4388175/1‑150 (MQ=255)
                                                                               ttattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAAt                                                                        <  1:1715369/150‑1 (MQ=255)
                                                                               ttattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAAt                                                                        >  2:2211485/1‑150 (MQ=255)
                                                                                tattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAAtt                                                                       <  2:2490912/150‑1 (MQ=255)
                                                                                    cATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGc                                                                   <  1:1296146/150‑1 (MQ=255)
                                                                                       atCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAAt                                                                <  2:1905536/150‑1 (MQ=255)
                                                                                          cAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATaaa                                                             >  2:5218596/1‑150 (MQ=255)
                                                                                           aaaGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAAt                                                            <  2:2559028/150‑1 (MQ=255)
                                                                                            aaGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAAtt                                                           >  2:1614906/1‑150 (MQ=255)
                                                                                               aaTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGAt                                                        >  2:3127428/1‑150 (MQ=255)
                                                                                                aTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                       <  1:1533106/150‑1 (MQ=255)
                                                                                                 tgtgTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCg                                                      <  2:583227/150‑1 (MQ=255)
                                                                                                   tttttAAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATTATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGt                                                    <  2:4771049/148‑1 (MQ=255)
                                                                                                   tgtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTATCCTTGTCAGGGGAGAAACTATGATTATAAATTAAATATTGATCACAAGTATGCACAATTTCCTGAGGACAAAATTCAGCAATAACTTGagaggt                                                    >  2:3666429/1‑145 (MQ=255)
                                                                                                   tgtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGt                                                    >  1:2409532/1‑150 (MQ=255)
                                                                                                   tgtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGt                                                    >  2:2664143/1‑150 (MQ=255)
                                                                                                     tttCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaa                                                  <  2:3253719/150‑1 (MQ=255)
                                                                                                      ttCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaaa                                                 >  1:4574892/1‑150 (MQ=255)
                                                                                                       tCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaaaa                                                >  2:1067560/1‑150 (MQ=255)
                                                                                                         aaaCATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaaaaaa                                              >  1:3753084/1‑150 (MQ=255)
                                                                                                          aaCATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAac                                             <  1:3886038/150‑1 (MQ=255)
                                                                                                           aCATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTTTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAaca                                            <  2:1176320/150‑1 (MQ=255)
                                                                                                           aCATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAaca                                            <  2:3795780/150‑1 (MQ=255)
                                                                                                            cATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAacac                                           <  1:545375/150‑1 (MQ=255)
                                                                                                               tttttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACaaa                                        >  1:4912018/1‑150 (MQ=255)
                                                                                                                ttttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACaaaa                                       >  1:1252386/1‑150 (MQ=255)
                                                                                                                 tttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAAc                                      <  2:4524578/150‑1 (MQ=255)
                                                                                                                     aaCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCtt                                  >  2:2359775/1‑150 (MQ=255)
                                                                                                                       cTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTat                                <  1:786547/150‑1 (MQ=255)
                                                                                                                        tAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAAAATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatc                               >  2:1548833/1‑150 (MQ=255)
                                                                                                                         aaaTAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatca                              >  1:273147/1‑150 (MQ=255)
                                                                                                                          aataatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAATCGCTTatcag                             >  2:5176777/1‑149 (MQ=255)
                                                                                                                          aataatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatcat                             >  1:2155807/1‑150 (MQ=255)
                                                                                                                             aatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATcatcat                          >  2:2368860/1‑150 (MQ=255)
                                                                                                                                 tCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGaaa                      >  1:4142510/1‑150 (MQ=255)
                                                                                                                                    ggCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATGGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGt                   >  2:2807948/1‑150 (MQ=255)
                                                                                                                                     gCTTGTTCTCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTa                  <  2:2086581/150‑1 (MQ=255)
                                                                                                                                       tttttCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAat                <  2:3740827/147‑1 (MQ=255)
                                                                                                                                           tCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATgg            <  2:1592844/150‑1 (MQ=255)
                                                                                                                                           tCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATgg            <  1:2988578/150‑1 (MQ=255)
                                                                                                                                             gCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAc          <  1:4108562/150‑1 (MQ=255)
                                                                                                                                               aGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACAt        <  1:3829039/150‑1 (MQ=255)
                                                                                                                                                 aTTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTc      <  1:4369107/150‑1 (MQ=255)
                                                                                                                                                     cGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCata  <  2:1634359/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATA  >  CP009273/2988625‑2988923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑