Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,989,295 C→A 61.8% pseudogene (85/261 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,989,2950CA61.8% 229.3 / inf 321pseudogene (85/261 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base C (71/52);  new base A (95/103);  total (166/155)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

CAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAA  >  CP009273/2989146‑2989444
                                                                                                                                                     |                                                                                                                                                     
  aGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACtatttatt                                                                                                                                                     >  1:2933262/1‑150 (MQ=255)
    ggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttctttc                                                                                                                                                   >  1:4120059/1‑150 (MQ=255)
     gTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCa                                                                                                                                                  <  1:213705/150‑1 (MQ=255)
       ataAAATTATCCATCTCATAAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTACTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAAt                                                                                                                                                <  2:4963013/150‑1 (MQ=255)
        taAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                               <  2:1016614/150‑1 (MQ=255)
             ttATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCa                                                                                                                                          >  2:4295971/1‑150 (MQ=255)
             ttATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCa                                                                                                                                          >  2:4291837/1‑150 (MQ=255)
                     ctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAAtttt                                                                                                                                  >  2:4846063/1‑150 (MQ=255)
                     ctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAtttt                                                                                                                                  >  2:3865784/1‑150 (MQ=255)
                        atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCt                                                                                                                               <  1:2420145/150‑1 (MQ=255)
                         tcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCtt                                                                                                                              >  1:1001308/1‑150 (MQ=255)
                          caATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCttt                                                                                                                             <  2:4200732/150‑1 (MQ=255)
                           aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtttt                                                                                                                            <  2:114464/150‑1 (MQ=255)
                           aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtttt                                                                                                                            >  2:3130400/1‑150 (MQ=255)
                           aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtttt                                                                                                                            >  1:3000858/1‑150 (MQ=255)
                            ataatTCCTTTTGTATAGCAATCATAATAACCTTGAATAAATTTTTCTTCTATCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTa                                                                                                                           <  2:647335/150‑1 (MQ=255)
                            ataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAATTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGGTTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTa                                                                                                                           <  2:1709692/150‑1 (MQ=255)
                             taatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTaa                                                                                                                          >  1:3338444/1‑150 (MQ=255)
                               ctGCCTTTTGTATAGCCAACATAATAACCTTGAATAAATGTTTCTGCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                        <  1:1203554/149‑1 (MQ=255)
                                tGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATca                                                                                                                       >  2:3399040/1‑150 (MQ=255)
                                 gacTTTTGTATAGCCATAATAATAACCTTGAATTAATTTTTCTTCTCTATTTTACTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcac                                                                                                                      <  2:3318002/148‑1 (MQ=255)
                                 gCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcac                                                                                                                      >  1:1594280/1‑150 (MQ=255)
                                  ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                     <  1:534118/150‑1 (MQ=255)
                                  ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                     >  1:350173/1‑150 (MQ=255)
                                   cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaa                                                                                                                    >  1:4323525/1‑150 (MQ=255)
                                   cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaa                                                                                                                    >  1:3170749/1‑150 (MQ=255)
                                    ttttGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGACATTTTTCTTTTAATCacaac                                                                                                                   >  1:2711123/1‑150 (MQ=255)
                                       tGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACAcc                                                                                                                >  1:753370/1‑150 (MQ=255)
                                       tGTATAGACATCATAATAACCTTTAATAAATTTTTCTTCTATCTTTTCCTAAATACTCTGAATTTTTTAGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACAcc                                                                                                                <  2:3855010/150‑1 (MQ=255)
                                        gTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATAAGAAATTTTTCTTTTAATCACAACACCa                                                                                                               >  2:4746949/1‑150 (MQ=255)
                                          ataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAtt                                                                                                             <  1:621979/150‑1 (MQ=255)
                                          ataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAtt                                                                                                             >  1:403615/1‑150 (MQ=255)
                                            aGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTTAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTg                                                                                                           <  1:1367412/150‑1 (MQ=255)
                                                atcatAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAgg                                                                                                       >  2:2475473/1‑150 (MQ=255)
                                                  catAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgag                                                                                                     >  1:405066/1‑150 (MQ=255)
                                                   ataataACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgaga                                                                                                    >  1:4736044/1‑150 (MQ=255)
                                                       taaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAtttt                                                                                                <  1:1049725/150‑1 (MQ=255)
                                                         aCCTTTAATAATTTTTTCTTCTATATTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAATTATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTAt                                                                                              <  2:180674/150‑1 (MQ=255)
                                                             tGAATAAATTTTTCGTCTCTCTTTTCCTCAATACTCTGTATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAg                                                                                          <  1:867343/150‑1 (MQ=255)
                                                              gAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCCCAACACCATTTGCAGGAGATTTTATCAAGc                                                                                         >  2:2363941/1‑150 (MQ=255)
                                                                   aaTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtc                                                                                    <  1:1393312/150‑1 (MQ=255)
                                                                    aTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCTGTGTTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtct                                                                                   <  2:1983148/150‑1 (MQ=255)
                                                                    aTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtct                                                                                   <  1:3621818/150‑1 (MQ=255)
                                                                    aTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtct                                                                                   <  1:1626698/150‑1 (MQ=255)
                                                                      ttttCTTCTCTCTTTTAATCAATACTCTGTATTTTTGATGTGTTTTCAAGAATTTTTTTTTCTTTAAGAGAAACTATTTATTTCAATACATAAGTAATTTTTCTTTTAATCACAACACCATTTGATGGAGATTTTATCAAGATAATctct                                                                                 <  2:3695880/150‑1 (MQ=255)
                                                                        ttattctCTCTTTTCCTAAATACTATGAATTTTTGAGGTGTTTTCAATAAGTATTTTTTCTTTAATAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAt                                                                               <  2:4610899/147‑1 (MQ=255)
                                                                         tcttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtt                                                                              <  2:2560514/150‑1 (MQ=255)
                                                                         tcttctCTCGTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtt                                                                              <  2:1441536/150‑1 (MQ=255)
                                                                          tttctCACTTTTCCTGACTCATCTGTTTTTTTTCGGTGTTTTCTAGTAGTATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCAAAAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttt                                                                             <  2:2825371/149‑1 (MQ=255)
                                                                           ttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttt                                                                            <  1:256323/150‑1 (MQ=255)
                                                                           ttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttt                                                                            >  1:4536595/1‑150 (MQ=255)
                                                                            tctctcTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttt                                                                           <  1:4967514/150‑1 (MQ=255)
                                                                              tctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttttt                                                                         >  1:399065/1‑150 (MQ=255)
                                                                              tctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttttt                                                                         <  1:2315839/150‑1 (MQ=255)
                                                                               ctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttattttt                                                                        >  2:3558487/1‑150 (MQ=255)
                                                                               ctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttttt                                                                        >  1:383069/1‑150 (MQ=255)
                                                                                tctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTc                                                                       <  1:4679528/150‑1 (MQ=255)
                                                                                tctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTc                                                                       <  2:703990/150‑1 (MQ=255)
                                                                                 ctTTTCCTCAATTCTATGAATTTTTGCGGTTGTTTCAAGTAGGTTTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCg                                                                      <  2:1049458/150‑1 (MQ=255)
                                                                                 ctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCg                                                                      >  1:4237277/1‑150 (MQ=255)
                                                                                     tCCTCAATACTCTTAATTTTTTCGGTGGTTTCAATAATGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAAAACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATa                                                                  <  2:5195256/150‑1 (MQ=255)
                                                                                     tCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAATTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATa                                                                  >  2:5115167/1‑150 (MQ=255)
                                                                                      ccTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATaa                                                                 <  1:1530744/150‑1 (MQ=255)
                                                                                      ccTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCATGCTCATCTCTATTTTTTGTCGCATaa                                                                 >  2:3233572/1‑150 (MQ=255)
                                                                                          aaTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATaaaagg                                                             >  1:4908323/1‑148 (MQ=255)
                                                                                          aaTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCAAAACACCATTAGCAGGAGATTTTATCAAGATCATCACTCTTTTTTTTCGCaaaaaagt                                                             >  2:3386010/1‑148 (MQ=255)
                                                                                           aTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGt                                                            <  1:4161781/150‑1 (MQ=255)
                                                                                            tACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTa                                                           >  2:2605514/1‑150 (MQ=255)
                                                                                             aatctttaTTTTTGCGTTGGTTTCAAGAATGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCATAAATTTTTCTTTTAATCACAACACCATTTGCAGTATATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTaa                                                          <  2:4393265/143‑1 (MQ=255)
                                                                                             aCTATGAATTTTTGCGTTGTTTTCAATAAGGATTTTTTATTTAAGAGAAACTATTTCTTTCAATAAATAATAAATTTTTATTTTAATCAAAACACCATTTGCAGGTTATTTTATCAAGCTCATCTATATTTTTTTTCGCATAAAATGTaa                                                          <  2:360196/150‑1 (MQ=255)
                                                                                                ctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtc                                                       >  2:3788636/1‑150 (MQ=255)
                                                                                                 tGAATTTTTGCGGTGGTTTCAATAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtct                                                      <  2:1942977/150‑1 (MQ=255)
                                                                                                    aTTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtctctc                                                   >  1:510320/1‑150 (MQ=255)
                                                                                                    aTTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtctctc                                                   <  1:1783742/150‑1 (MQ=255)
                                                                                                        ttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTaaa                                               >  1:3132430/1‑150 (MQ=255)
                                                                                                            ggtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtg                                           <  1:1268937/150‑1 (MQ=255)
                                                                                                            ggtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtg                                           >  1:1910725/1‑150 (MQ=255)
                                                                                                             gtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtgt                                          >  2:1974849/1‑150 (MQ=255)
                                                                                                              ttgtTTCAAGAAGGATTTTTTCTTTAATAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGtt                                         <  2:4129115/148‑1 (MQ=255)
                                                                                                                gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGa                                       >  2:5106778/1‑150 (MQ=255)
                                                                                                                gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCTTCTGAAAGTTTTCATTTAATCACAACACCATTTGCAGGAGGTTTTTACGAGCTCCTGTCTATTTTTGTTAGCATCAGATTTAATACACTGTATGAGGGTTGa                                       >  2:2759106/1‑150 (MQ=255)
                                                                                                                gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTCATTCTCTCTAAAAGTGGTGa                                       >  2:1886664/1‑150 (MQ=255)
                                                                                                                 tttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                      <  1:451324/150‑1 (MQ=255)
                                                                                                                   tCAAGAAGGATTTTTTCTTTAATAGAAACTATTTCTTTAAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGc                                    <  1:4291942/150‑1 (MQ=255)
                                                                                                                   tCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGc                                    >  2:402158/1‑150 (MQ=255)
                                                                                                                        aagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTg                               >  2:4077885/1‑150 (MQ=255)
                                                                                                                          ggATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGt                             >  1:14722/1‑150 (MQ=255)
                                                                                                                          ggATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTATCTAATAGTGTTGATGCTAGTGGt                             >  1:3666051/1‑150 (MQ=255)
                                                                                                                            aTTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                           <  1:2910891/150‑1 (MQ=255)
                                                                                                                             ttttttCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAAc                          >  1:5182122/1‑150 (MQ=255)
                                                                                                                             ttttttCTTTAAGAGAAACTATTTATTTAAATCAATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAAc                          <  2:1640078/150‑1 (MQ=255)
                                                                                                                               ttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACgg                        >  2:1282292/1‑150 (MQ=255)
                                                                                                                                      tAATAGAAACTATTTATTTCAATACATCAGAAATTTTTCTTTTAATCACAACACCATTTTCATGATATTTTATCAAGCTCATCTCTATTTTTTTTCTCATAAATTGTAATTCTATCTAAAAGTTTTGATGCTATTGGTAACGGCGATTGt                 <  2:325813/150‑1 (MQ=255)
                                                                                                                                      tAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt                 >  2:2271290/1‑150 (MQ=255)
                                                                                                                                           gaAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgtg            >  1:3766755/1‑150 (MQ=255)
                                                                                                                                            aaaCTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTgg           >  1:3779825/1‑150 (MQ=255)
                                                                                                                                             aaCTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGt          >  1:2209619/1‑150 (MQ=255)
                                                                                                                                                 atttatttCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTcaac      <  1:3712363/150‑1 (MQ=255)
                                                                                                                                                 aTTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTcaac      <  1:1818596/150‑1 (MQ=255)
                                                                                                                                                     ctttcAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACaaaa  <  2:2999526/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAA  >  CP009273/2989146‑2989444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑