Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,111,434 C→A 69.0% A346A (GCG→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,111,4340CA69.0% 425.7 / inf 329A346A (GCG→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (68/34);  new base A (136/91);  total (204/125)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 330 total aligned reads displayed.

CAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACGGCC  >  CP009273/3111287‑3111583
                                                                                                                                                   |                                                                                                                                                     
   cGTCGCCCCGTTGGAGATCGAGAGATTGATAATCGCGTTGATCTCGTTGGGTTCTGCGGCAATGACCTTGCGTACTTCGTCCGGAACAACTCGGGTATTATTTTGCCCGGTCGTCGAATAGAGATTAATATGCTGATCAATATTCGcccc                                                                                                                                                  <  2:4379748/150‑1 (MQ=255)
   cGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGcccc                                                                                                                                                  >  2:5041537/1‑150 (MQ=255)
      cGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCcgcg                                                                                                                                               <  1:5041537/150‑1 (MQ=255)
        cACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGaa                                                                                                                                             >  2:3468464/1‑150 (MQ=255)
         aCCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAAc                                                                                                                                            <  2:2855693/150‑1 (MQ=255)
           cGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACtt                                                                                                                                          >  2:1881357/1‑150 (MQ=255)
           cGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACtt                                                                                                                                          >  1:2071731/1‑150 (MQ=255)
            gTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtc                                                                                                                                         >  2:1866270/1‑150 (MQ=255)
            gTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtc                                                                                                                                         <  2:3551257/150‑1 (MQ=255)
            gTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtc                                                                                                                                         <  2:4347381/150‑1 (MQ=255)
            gTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtc                                                                                                                                         <  1:1253354/150‑1 (MQ=255)
             ttGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtca                                                                                                                                        <  2:3111981/150‑1 (MQ=255)
              tGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtcat                                                                                                                                       <  1:5216352/150‑1 (MQ=255)
               ggATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtcatc                                                                                                                                      >  2:789907/1‑150 (MQ=255)
                tctAACGATTGATTGATACTCACGTTGAGCACGTTTGGATCTTCTGCAAAGCCCTTTCTTTCATCGTCCGCAACAACACGTGTATTATTTTGCCCGGTCGTCGAATAGCGATGTATAAGCTGATCAATATTCGCCCCGCGAACTtcatca                                                                                                                                     <  2:2816775/148‑1 (MQ=255)
                 aTAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAc                                                                                                                                    >  2:164778/1‑150 (MQ=255)
                  tAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAcc                                                                                                                                   <  2:1514956/150‑1 (MQ=255)
                   aaCGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCAccc                                                                                                                                  >  1:2888910/1‑150 (MQ=255)
                      gagagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGt                                                                                                                               >  1:326052/1‑150 (MQ=255)
                      gagagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGt                                                                                                                               >  2:56050/1‑150 (MQ=255)
                       agagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCACCGCGAACTTCATCACCCAGtt                                                                                                                              >  1:1077106/1‑150 (MQ=255)
                        gagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtc                                                                                                                             <  2:1371072/150‑1 (MQ=255)
                        gagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtc                                                                                                                             >  2:4648659/1‑150 (MQ=255)
                           aTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTtcagt                                                                                                                          >  2:2366855/1‑150 (MQ=255)
                              gATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCATTATTCGCCCCGCGAACTTCATCACCCAGTtcagtcag                                                                                                                       >  2:2762498/1‑150 (MQ=255)
                              gATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTtcagtcag                                                                                                                       >  1:1932151/1‑150 (MQ=255)
                                    ctcGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAAt                                                                                                                 <  1:4634741/150‑1 (MQ=255)
                                       gTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGt                                                                                                              >  1:177945/1‑150 (MQ=255)
                                       gTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCGCCCAGTTCAGTCAGCGCACTGGt                                                                                                              >  1:4888687/1‑150 (MQ=255)
                                       gTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGt                                                                                                              >  1:363557/1‑150 (MQ=255)
                                        ttGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTc                                                                                                             <  1:3810786/150‑1 (MQ=255)
                                        ttGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTc                                                                                                             <  1:3249242/150‑1 (MQ=255)
                                         tGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCg                                                                                                            >  1:3680766/1‑150 (MQ=255)
                                          gATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGa                                                                                                           <  2:1750215/150‑1 (MQ=255)
                                          gATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGa                                                                                                           >  1:3146836/1‑150 (MQ=255)
                                            tCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACt                                                                                                         >  1:3862850/1‑150 (MQ=255)
                                            tCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACt                                                                                                         >  1:5073609/1‑150 (MQ=255)
                                                gTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAtt                                                                                                     >  1:813460/1‑150 (MQ=255)
                                                    ggATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcg                                                                                                 >  2:3887021/1‑150 (MQ=255)
                                                      atatTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcgcg                                                                                               >  2:1321343/1‑150 (MQ=255)
                                                        atTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCa                                                                                             >  1:2511302/1‑150 (MQ=255)
                                                             gCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGaa                                                                                        <  2:3394668/150‑1 (MQ=255)
                                                              cAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAc                                                                                       >  1:3980677/1‑150 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                      >  1:4835368/1‑150 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                      >  2:4298877/1‑150 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                      >  1:2149249/1‑150 (MQ=255)
                                                               aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTAGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCACTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCCCTGAAcc                                                                                      >  2:90204/1‑150 (MQ=255)
                                                                 aGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCa                                                                                    <  1:4588952/150‑1 (MQ=255)
                                                                 aGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCa                                                                                    >  1:3776804/1‑150 (MQ=255)
                                                                  gACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAg                                                                                   >  1:890873/1‑150 (MQ=255)
                                                                  gACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAg                                                                                   <  2:1927587/150‑1 (MQ=255)
                                                                     cTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTc                                                                                >  2:104662/1‑150 (MQ=255)
                                                                         cGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAGTGCCGCGCACTGAACCCAGTTCAAAg                                                                            >  1:4041761/1‑150 (MQ=255)
                                                                          gTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAgg                                                                           >  1:1207773/1‑150 (MQ=255)
                                                                          gTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAgg                                                                           >  2:4522274/1‑150 (MQ=255)
                                                                             cATCGTCCGGAACAACACGGGTATTATTGTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                        <  2:3299505/150‑1 (MQ=255)
                                                                              aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATc                                                                       >  1:4721130/1‑150 (MQ=255)
                                                                                     ggAACAACACGGGTATTATTGTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCa                                                                <  2:4623286/150‑1 (MQ=255)
                                                                                      gAACAACACGGGTATTATTTTGCCGGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaa                                                               >  1:1073104/1‑150 (MQ=255)
                                                                                        acaacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAg                                                             >  2:3839142/1‑150 (MQ=255)
                                                                                         caacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAgg                                                            >  1:3213297/1‑150 (MQ=255)
                                                                                         caacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAgg                                                            >  2:4414639/1‑150 (MQ=255)
                                                                                             acGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAt                                                        >  2:221942/1‑150 (MQ=255)
                                                                                             acGGGTATTATTTGGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAt                                                        <  1:4413648/150‑1 (MQ=255)
                                                                                                  tattatTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGttt                                                   >  1:2724200/1‑150 (MQ=255)
                                                                                                  tattatTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGttt                                                   <  2:846862/150‑1 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                                  >  2:201895/1‑150 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCAACCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                                  >  2:2168486/1‑150 (MQ=255)
                                                                                                   attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                                  <  1:131205/150‑1 (MQ=255)
                                                                                                    ttattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCg                                                 <  2:4048066/150‑1 (MQ=255)
                                                                                                      attTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCACAGGAGATGGTTTCGcc                                               >  2:4852154/1‑150 (MQ=255)
                                                                                                         ttGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCa                                            <  1:4971274/150‑1 (MQ=255)
                                                                                                           gCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGc                                          <  1:1998603/150‑1 (MQ=255)
                                                                                                                 tcgtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAgg                                    <  1:3592461/150‑1 (MQ=255)
                                                                                                                  cgtcgAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGa                                   >  1:2126560/1‑150 (MQ=255)
                                                                                                                   gtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaa                                  >  1:298944/1‑150 (MQ=255)
                                                                                                                    tcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaa                                 >  1:2831959/1‑150 (MQ=255)
                                                                                                                     cgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaaa                                >  2:2938656/1‑150 (MQ=255)
                                                                                                                         tAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACACAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCACTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCa                            <  2:62996/150‑1 (MQ=255)
                                                                                                                            cGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCGCCa                         >  1:4316430/1‑150 (MQ=255)
                                                                                                                            cGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCa                         >  2:4883954/1‑150 (MQ=255)
                                                                                                                             gATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAt                        <  1:105795/150‑1 (MQ=255)
                                                                                                                             gATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAt                        >  1:1707095/1‑150 (MQ=255)
                                                                                                                             gATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAt                        >  2:65145/1‑150 (MQ=255)
                                                                                                                                gAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAtttt                     <  2:4677730/150‑1 (MQ=255)
                                                                                                                                 aaTAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTc                    >  1:2357259/1‑150 (MQ=255)
                                                                                                                                   tAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTccc                  <  2:4244541/150‑1 (MQ=255)
                                                                                                                                    aaGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc                 <  1:2316013/150‑1 (MQ=255)
                                                                                                                                     aGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCt                <  2:4417833/150‑1 (MQ=255)
                                                                                                                                     aGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACAAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCt                >  2:1241795/1‑150 (MQ=255)
                                                                                                                                       cTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAATGAAAATTCACCATTTTCCCCTGt              >  2:5180143/1‑150 (MQ=255)
                                                                                                                                        tGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTc             >  1:3909405/1‑150 (MQ=255)
                                                                                                                                        tGAACAATATTAGCCCCGCGAACTTCATCACCCAGTACAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTc             <  1:3762072/150‑1 (MQ=255)
                                                                                                                                           tCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACg          <  2:2908761/150‑1 (MQ=255)
                                                                                                                                            cAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGc         >  1:3382779/1‑150 (MQ=255)
                                                                                                                                             aaTATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc        >  1:1104690/1‑150 (MQ=255)
                                                                                                                                             aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc        <  1:3035737/150‑1 (MQ=255)
                                                                                                                                             aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc        >  2:252460/1‑150 (MQ=255)
                                                                                                                                               tatTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAc      <  2:307178/150‑1 (MQ=255)
                                                                                                                                                 ttCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACgg    >  2:2268256/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAGCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCACGGCC  >  CP009273/3111287‑3111583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑