Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,246,908 G→A 15.5% R222R (CGC→CGT)  yhaJ ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,246,9080GA15.5% 824.1 / 117.9 297R222R (CGC→CGT) yhaJLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (115/133);  new base A (26/20);  total (144/153)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

ATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGTAGAC  >  CP009273/3246759‑3247057
                                                                                                                                                     |                                                                                                                                                     
 taataTCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTCAAgc                                                                                                                                                      >  1:2383753/1‑150 (MQ=255)
  aataTCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcg                                                                                                                                                     <  2:3286349/150‑1 (MQ=255)
     atCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCACTCGTGCTCACCGTTAAGCGCgg                                                                                                                                                  >  2:3115430/1‑150 (MQ=255)
       cGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCt                                                                                                                                                <  2:3509762/150‑1 (MQ=255)
       cGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCATTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCt                                                                                                                                                <  1:4032499/150‑1 (MQ=255)
         aTCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGt                                                                                                                                              >  1:3877288/1‑150 (MQ=255)
           ctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGttt                                                                                                                                            >  1:4179766/1‑150 (MQ=255)
           ctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGttt                                                                                                                                            <  2:4494968/150‑1 (MQ=255)
             cGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                                                                                                                                          >  1:3750589/1‑150 (MQ=255)
             cGCTGGTCGAGTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                                                                                                                                          <  2:3701430/150‑1 (MQ=255)
                tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcac                                                                                                                                       >  2:2899444/1‑148 (MQ=255)
                tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCca                                                                                                                                       <  2:2232778/150‑1 (MQ=255)
                  gTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCcagc                                                                                                                                     >  1:1935240/1‑150 (MQ=255)
                    cGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagcag                                                                                                                                   >  2:4902034/1‑150 (MQ=255)
                     gATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTGTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCagcagc                                                                                                                                  >  2:1544172/1‑150 (MQ=255)
                         ccGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                                                                                                                              <  1:2484249/150‑1 (MQ=255)
                         ccGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                                                                                                                              <  1:1926843/150‑1 (MQ=255)
                          cGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTAc                                                                                                                             >  1:2339152/1‑150 (MQ=255)
                              cTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTc                                                                                                                         >  1:4753715/1‑150 (MQ=255)
                                gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCaa                                                                                                                       >  1:4384615/1‑150 (MQ=255)
                                    acacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACAcc                                                                                                                   >  2:3999641/1‑150 (MQ=255)
                                        gCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGgc                                                                                                               >  1:23068/1‑150 (MQ=255)
                                         cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGgcg                                                                                                              <  1:1020671/150‑1 (MQ=255)
                                         cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGgcg                                                                                                              >  2:1758810/1‑150 (MQ=255)
                                         cAACCGCCCTTCCGCAATATCTTATTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGACTGACGTTTATCTTCAATCTTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGAAGCTGTACGGTCAACACCGGgcg                                                                                                              >  2:3950297/1‑150 (MQ=255)
                                          aaCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGgcgc                                                                                                             >  2:5159535/1‑150 (MQ=255)
                                           aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCt                                                                                                            >  1:1408554/1‑150 (MQ=255)
                                             cGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCa                                                                                                          >  2:1894591/1‑150 (MQ=255)
                                             cGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCa                                                                                                          <  2:2342068/150‑1 (MQ=255)
                                             cGCCCTTCCGCAATATCTTATTCGACCATAGGATACGTCATCGTCGACAAGCAAAGCCCCGCCAGTAAAGCCTGAAGATTAACAACAAACGTGCTCACCGATAATCGCGGCTGTTTGTCAAGCAGCTATACGGTCAAAAACGGTCGCTCa                                                                                                          >  1:3173266/1‑150 (MQ=255)
                                              gCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCACCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCAc                                                                                                         >  2:855584/1‑150 (MQ=255)
                                               cccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACg                                                                                                        <  1:3525880/150‑1 (MQ=255)
                                                ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGa                                                                                                       >  2:467401/1‑150 (MQ=255)
                                                ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGa                                                                                                       >  2:3008427/1‑150 (MQ=255)
                                                 cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAg                                                                                                      <  1:2725511/150‑1 (MQ=255)
                                                   tCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCg                                                                                                    >  1:2245924/1‑150 (MQ=255)
                                                     cGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGt                                                                                                  <  2:1102261/150‑1 (MQ=255)
                                                          tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGCCGTTTATCTTCAATCGTGCTCACCGTTAATCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACGGGGCGCTCACGAGCGGTATCCg                                                                                             >  2:3671255/1‑150 (MQ=255)
                                                           atCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGc                                                                                            >  2:3270246/1‑150 (MQ=255)
                                                           atCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGc                                                                                            >  1:1901545/1‑150 (MQ=255)
                                                            tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGcc                                                                                           >  1:996832/1‑150 (MQ=255)
                                                                tttCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCCCCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCg                                                                                       >  2:1369300/1‑150 (MQ=255)
                                                                tttCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCg                                                                                       <  1:179424/150‑1 (MQ=255)
                                                                  tCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCa                                                                                     >  2:1590336/1‑150 (MQ=255)
                                                                   cGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCaa                                                                                    >  2:3076875/1‑150 (MQ=255)
                                                                      ccATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTc                                                                                 <  2:4812111/150‑1 (MQ=255)
                                                                       cATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTcc                                                                                <  2:4165195/150‑1 (MQ=255)
                                                                        aTAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCa                                                                               <  1:2935813/150‑1 (MQ=255)
                                                                         tAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCAc                                                                              <  1:3597918/150‑1 (MQ=255)
                                                                         tAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCAc                                                                              >  1:1883686/1‑150 (MQ=255)
                                                                          aGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACg                                                                             >  2:349796/1‑150 (MQ=255)
                                                                           ggATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGa                                                                            <  1:1139792/150‑1 (MQ=255)
                                                                           ggATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGa                                                                            <  1:3756585/150‑1 (MQ=255)
                                                                           ggATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGa                                                                            >  1:5186392/1‑150 (MQ=255)
                                                                             aTACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGata                                                                          <  2:2050295/150‑1 (MQ=255)
                                                                                 ggCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACAGCAATTCCACGATATTTc                                                                      >  2:685015/1‑150 (MQ=255)
                                                                                   cATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTcac                                                                    <  1:4778303/150‑1 (MQ=255)
                                                                                    aTCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGGATCCGCCACCGCAATTCCACGATATTTcaca                                                                   >  2:3313705/1‑150 (MQ=255)
                                                                                      cgtcgCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACAcg                                                                 >  1:724999/1‑150 (MQ=255)
                                                                                       gtcgCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACAcgc                                                                >  1:3293086/1‑150 (MQ=255)
                                                                                        tcgCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACAcgcg                                                               <  2:4765523/150‑1 (MQ=255)
                                                                                        tcgCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACAcgcg                                                               >  2:4073640/1‑150 (MQ=255)
                                                                                         cgccacgccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGt                                                              >  1:4077211/1‑150 (MQ=255)
                                                                                           ccacgccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCa                                                            <  2:5201748/150‑1 (MQ=255)
                                                                                            cacgccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCAc                                                           <  2:1461947/150‑1 (MQ=255)
                                                                                              cgccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACtt                                                         >  1:4441965/1‑150 (MQ=255)
                                                                                              cgccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACtt                                                         <  1:3638898/150‑1 (MQ=255)
                                                                                               gccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTc                                                        <  2:5153542/150‑1 (MQ=255)
                                                                                                ccaAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCa                                                       <  1:1504958/150‑1 (MQ=255)
                                                                                                 caAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAg                                                      >  2:3173482/1‑150 (MQ=255)
                                                                                                  aaGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGa                                                     >  1:2640353/1‑150 (MQ=255)
                                                                                                   aGCCCCGCAATTTATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTTTCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGAt                                                    <  1:4902895/150‑1 (MQ=255)
                                                                                                     ccccGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATaa                                                  <  2:252479/150‑1 (MQ=255)
                                                                                                     ccccGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATaa                                                  <  2:4609633/150‑1 (MQ=255)
                                                                                                      cccGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATaaa                                                 <  2:211026/150‑2 (MQ=255)
                                                                                                       ccGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACg                                                <  1:478297/150‑1 (MQ=255)
                                                                                                             gtatttccTGACGTTTATCTTAATTAGTGCTCACCTTTAAGCTCGGCTGTTTGTACATCAGCTGTACGGTCAACACCGGGAGATAACGAGCTGTATCCGCAAACGAATTTCCTCGATATTTCACACGCGTCACTTCAGATAACGGTTCCg                                          <  2:943848/144‑1 (MQ=255)
                                                                                                             gTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCg                                          >  1:1197580/1‑150 (MQ=255)
                                                                                                             gTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCg                                          >  1:2008487/1‑150 (MQ=255)
                                                                                                             gTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCg                                          <  1:2589562/150‑1 (MQ=255)
                                                                                                              tAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCgg                                         <  1:1755027/150‑1 (MQ=255)
                                                                                                               aaTGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGc                                        <  2:4643027/150‑1 (MQ=255)
                                                                                                                     tGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCtga                                  <  2:1006781/150‑1 (MQ=255)
                                                                                                                     tGACGTTTATCTTCAATCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCtga                                  >  2:3722904/1‑150 (MQ=255)
                                                                                                                         gTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGaa                              >  2:1197008/1‑150 (MQ=255)
                                                                                                                                 tCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAAAGGTTCCGGCTCCTGATGAATCGGGTGa                      >  1:4626046/1‑150 (MQ=255)
                                                                                                                                  cAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGAt                     >  2:2314135/1‑150 (MQ=255)
                                                                                                                                   aagcGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATc                    <  2:4564964/147‑1 (MQ=255)
                                                                                                                                    aTCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCa                   <  1:1590336/150‑1 (MQ=255)
                                                                                                                                     tCGTGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACCCGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAg                  <  2:260717/150‑1 (MQ=255)
                                                                                                                                        tGCTCACCGTTAAACGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGgcg               >  2:62066/1‑150 (MQ=255)
                                                                                                                                           tCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCgg            >  2:1979298/1‑150 (MQ=255)
                                                                                                                                            cACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGc           <  1:4281734/150‑1 (MQ=255)
                                                                                                                                            cACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGc           <  1:3988039/150‑1 (MQ=255)
                                                                                                                                                gTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACg       <  2:2836271/150‑1 (MQ=255)
                                                                                                                                                 ttAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGt      <  1:824374/150‑1 (MQ=255)
                                                                                                                                                  tAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGTa     <  1:2775542/150‑1 (MQ=255)
                                                                                                                                                     gcgcGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGTAGAc  <  2:2420786/150‑1 (MQ=255)
                                                                                                                                                     gcgcGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGTAGAc  <  1:5061183/150‑1 (MQ=255)
                                                                                                                                                     gcgcGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGTAGAc  <  1:3479409/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCTCACGAGCGGTATCCGCCACCGCAATTCCACGATATTTCACACGCGTCACTTCAGATAACGGTTCCGGCTCCTGATGAATCGGGTGATCAGGCGCGGCAACGTAGAC  >  CP009273/3246759‑3247057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑