Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,473,139 G→T 72.6% I276I (ATC→ATA)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,473,1390GT72.6% 460.7 / 275.7 306I276I (ATC→ATA) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (44/40);  new base T (110/112);  total (154/152)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

GCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGA  >  CP009273/3472994‑3473285
                                                                                                                                                 |                                                                                                                                                  
gCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATaa                                                                                                                                                >  1:3656206/1‑150 (MQ=255)
        tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACaaa                                                                                                                                        <  1:3480109/150‑1 (MQ=255)
         cttaGCTACGCACGCCATACAATCGCGCCAGCAGATACAGAACGAGAATTTTCTCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                       <  2:2078418/147‑1 (MQ=255)
         ctGAGCTACGCACGCCATCCACTCGCGCCAGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACACCCACGCTTATCACTACCCACACCAGATGGGTATAACGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACaccc                                                                                                                                       <  1:2571099/150‑4 (MQ=255)
         ctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGACGACAGATAGAAAGAACAAAc                                                                                                                                       >  1:945361/1‑150 (MQ=255)
          tGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAcc                                                                                                                                      >  1:1232172/1‑150 (MQ=255)
            aGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGa                                                                                                                                    >  1:3344363/1‑150 (MQ=255)
               gacgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagca                                                                                                                                 <  2:3371237/149‑1 (MQ=255)
                   cacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCaa                                                                                                                             <  1:4971179/150‑1 (MQ=255)
                     cgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGc                                                                                                                           >  1:505139/1‑150 (MQ=255)
                      gcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGcc                                                                                                                          >  1:2999570/1‑150 (MQ=255)
                       ccATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCt                                                                                                                         >  2:4416574/1‑150 (MQ=255)
                       ccATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCt                                                                                                                         >  2:3404777/1‑150 (MQ=255)
                         aTACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCttt                                                                                                                       >  1:599656/1‑150 (MQ=255)
                           aCATTCGCGCCAGCAGATACAGCAAGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGa                                                                                                                     <  2:2723016/150‑1 (MQ=255)
                           aCAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGa                                                                                                                     <  2:5162905/150‑1 (MQ=255)
                           aCAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGa                                                                                                                     <  1:5090924/150‑1 (MQ=255)
                            cAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGaa                                                                                                                    <  2:2880113/150‑1 (MQ=255)
                             aaTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                   <  1:707164/150‑1 (MQ=255)
                              aTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                                                                                                  >  2:4578121/1‑150 (MQ=255)
                                cgcgCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                                                                                                <  2:4281599/150‑1 (MQ=255)
                                 gcgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAt                                                                                                               <  2:2471551/150‑1 (MQ=255)
                                    ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGa                                                                                                            <  1:1067018/150‑1 (MQ=255)
                                     cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                                                                                                           <  1:3860102/150‑1 (MQ=255)
                                        cagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                                                                                                        >  1:4741771/1‑150 (MQ=255)
                                        cagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTAGAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                                                                                                        >  2:1180426/1‑150 (MQ=255)
                                         agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAAAAAACCGAGCAGCAAGCCTTTGAAGTGATCGATAGcc                                                                                                       >  2:696556/1‑150 (MQ=255)
                                          gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCg                                                                                                      >  2:5032925/1‑150 (MQ=255)
                                          gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCg                                                                                                      >  1:1331730/1‑150 (MQ=255)
                                               aGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTcc                                                                                                 >  1:2226924/1‑150 (MQ=255)
                                                        aTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg                                                                                        >  1:837971/1‑150 (MQ=255)
                                                          tttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCg                                                                                      >  1:2798889/1‑150 (MQ=255)
                                                          tttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCg                                                                                      <  1:1711858/150‑1 (MQ=255)
                                                             cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGata                                                                                   <  2:746476/150‑1 (MQ=255)
                                                              aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGatat                                                                                  >  1:3312534/1‑150 (MQ=255)
                                                                  ccaccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAc                                                                              <  1:3689237/150‑1 (MQ=255)
                                                                  ccaccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAc                                                                              <  1:4272419/150‑1 (MQ=255)
                                                                    accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACtt                                                                            <  2:3143735/150‑1 (MQ=255)
                                                                    accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACtt                                                                            >  1:1049224/1‑150 (MQ=255)
                                                                    accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACtt                                                                            >  2:3935172/1‑150 (MQ=255)
                                                                     ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACttt                                                                           <  2:327979/150‑1 (MQ=255)
                                                                     ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACttt                                                                           <  2:4734394/150‑1 (MQ=255)
                                                                      caGCACCACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCATTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                          <  1:2690170/150‑1 (MQ=255)
                                                                      caGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                          >  1:2450287/1‑150 (MQ=255)
                                                                       aGCACAACCACGCTTATCACTACCCACAACAGATGTGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTGCCAGTTCATGGCGATATTCACTTAcc                                                                         >  1:2633735/1‑150 (MQ=255)
                                                                        gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCg                                                                        <  2:2180505/150‑1 (MQ=255)
                                                                         cacaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGc                                                                       >  2:4098412/1‑150 (MQ=255)
                                                                          tcatcaaCGCTTATCAATACCCACAACATATGGGTATAAAGCACCCCGAGGTTGAGTGACATTCCGACAGATATAAAGACCAATCCGAGCATCAAGGCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                      <  2:3232595/144‑1 (MQ=255)
                                                                          acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                      >  2:4885327/1‑150 (MQ=255)
                                                                           caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCca                                                                     >  2:2595335/1‑150 (MQ=255)
                                                                           caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAGGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCca                                                                     <  1:411532/150‑1 (MQ=255)
                                                                            aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                    <  1:32525/150‑1 (MQ=255)
                                                                            aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                    >  2:2533173/1‑150 (MQ=255)
                                                                                aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                                >  2:1323682/1‑150 (MQ=255)
                                                                                  gCTTATCACTACCCACAACATATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCAc                                                              >  1:1283265/1‑150 (MQ=255)
                                                                                  gCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCAc                                                              >  1:3899441/1‑150 (MQ=255)
                                                                                  gCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCAc                                                              <  2:297408/150‑1 (MQ=255)
                                                                                     tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc                                                           >  1:4161868/1‑150 (MQ=255)
                                                                                      aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                          >  2:4464440/1‑150 (MQ=255)
                                                                                      aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                          <  1:1506440/150‑1 (MQ=255)
                                                                                           tACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAAt                                                     <  2:1669667/150‑1 (MQ=255)
                                                                                            aCCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATa                                                    <  2:2929540/150‑1 (MQ=255)
                                                                                              ccACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATaaa                                                  >  2:3634468/1‑150 (MQ=255)
                                                                                                 caacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGt                                               >  1:3864460/1‑150 (MQ=255)
                                                                                                   acaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAc                                             <  2:3051782/150‑1 (MQ=255)
                                                                                                   acaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAc                                             >  2:4448000/1‑150 (MQ=255)
                                                                                                    caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAcc                                            <  2:1517131/150‑1 (MQ=255)
                                                                                                    caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAcc                                            <  2:4818276/150‑1 (MQ=255)
                                                                                                    caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAAGGCGATATTAACTTTCAGCAAGCAGAACGCCCGCAATAAATTTAcc                                            >  2:2831923/1‑150 (MQ=255)
                                                                                                     aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                           <  1:4830566/150‑1 (MQ=255)
                                                                                                     aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                           <  1:2083537/150‑1 (MQ=255)
                                                                                                        tGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGAGATTCACTTTCCGCCAGCAGCACGCGCGCAATAAATGTACCGAgc                                        >  2:371302/1‑150 (MQ=255)
                                                                                                        tGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTGTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgc                                        >  2:1148465/1‑150 (MQ=255)
                                                                                                          ggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgcgc                                      >  1:1414264/1‑150 (MQ=255)
                                                                                                            tataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                    >  1:1795298/1‑150 (MQ=255)
                                                                                                            tataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                    >  2:561987/1‑150 (MQ=255)
                                                                                                            tataAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                    >  1:625258/1‑150 (MQ=255)
                                                                                                             ataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                   >  1:191940/1‑150 (MQ=255)
                                                                                                             ataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                   >  1:2898703/1‑150 (MQ=255)
                                                                                                               aaaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCg                                 <  1:4851664/150‑1 (MQ=255)
                                                                                                                aaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAGCGa                                >  2:3579868/1‑150 (MQ=255)
                                                                                                                    aCCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGc                            <  2:491860/150‑1 (MQ=255)
                                                                                                                     ccccGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcc                           <  2:2333450/150‑1 (MQ=255)
                                                                                                                         gAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAg                       <  1:998491/150‑1 (MQ=255)
                                                                                                                         gAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAg                       >  1:1001394/1‑150 (MQ=255)
                                                                                                                          aGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgc                      >  1:442902/1‑150 (MQ=255)
                                                                                                                              tGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCATTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCAt                  <  1:3224185/150‑1 (MQ=255)
                                                                                                                              tGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCAt                  >  1:203026/1‑150 (MQ=255)
                                                                                                                               gAGTGACATGCCGACAGATATAAAGAACACACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATc                 <  2:946742/150‑1 (MQ=255)
                                                                                                                               gAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATc                 >  1:567264/1‑150 (MQ=255)
                                                                                                                                aGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATcc                >  2:1921666/1‑150 (MQ=255)
                                                                                                                                     cATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaaa           >  1:1569701/1‑150 (MQ=255)
                                                                                                                                       tGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCCTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACa         >  2:1691833/1‑150 (MQ=255)
                                                                                                                                       tGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACa         >  1:5167373/1‑150 (MQ=255)
                                                                                                                                          cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg      >  1:2770896/1‑150 (MQ=255)
                                                                                                                                          cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg      >  2:1215197/1‑150 (MQ=255)
                                                                                                                                          cGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg      >  1:4659500/1‑150 (MQ=255)
                                                                                                                                           gACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGc     <  1:3447766/150‑1 (MQ=255)
                                                                                                                                           gACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGc     >  1:4185697/1‑150 (MQ=255)
                                                                                                                                              agatATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGAACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGa  >  2:1725563/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGA  >  CP009273/3472994‑3473285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑