BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,618,220	G→A	5.7%	M161I (ATG→ATA)	yhhI →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,618,220	0	G	A	5.7%	430.4 / 13.1	144	M161I (ATG→ATA)	yhhI	putative transposase
Reads supporting (aligned to +/- strand): ref base G (58/77); new base A (4/4); total (63/81)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 144 total aligned reads displayed.

GCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTT  >  CP009273/3618073‑3618367

gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCTTGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGTCGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCt                                                                                                                                                   <  1:3156740/150‑1 (MQ=21)

gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCt                                                                                                                                                   <  2:2043593/150‑1 (MQ=34)

gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCt                                                                                                                                                   <  1:1061300/150‑1 (MQ=34)

gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCt                                                                                                                                                   <  1:578877/150‑1 (MQ=34)

gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCt                                                                                                                                                   <  2:2043015/150‑1 (MQ=34)

gCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCCCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCt                                                                                                                                                   <  2:2210066/150‑1 (MQ=21)

 cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTg                                                                                                                                                  <  1:5159144/150‑1 (MQ=34)

 cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTg                                                                                                                                                  >  1:3760402/1‑150 (MQ=34)

 cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTg                                                                                                                                                  <  1:2567058/150‑1 (MQ=34)

 cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTg                                                                                                                                                  <  1:1291937/150‑1 (MQ=34)

 cTCAGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTg                                                                                                                                                  <  2:1931074/150‑1 (MQ=21)

 atCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTg                                                                                                                                                  >  2:1291749/2‑150 (MQ=21)

  tCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTgg                                                                                                                                                 <  1:587005/150‑1 (MQ=34)

  tCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATACTgg                                                                                                                                                 >  1:1717883/1‑150 (MQ=21)

  tCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATACTgg                                                                                                                                                 >  1:1717660/1‑150 (MQ=21)

   ccGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGa                                                                                                                                                >  1:1324606/1‑150 (MQ=34)

    cGGCACTCTTATGACTAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGat                                                                                                                                               <  2:2621011/150‑1 (MQ=21)

    cGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGat                                                                                                                                               >  2:1694251/1‑150 (MQ=34)

     ggCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGATGGata                                                                                                                                              >  2:182542/1‑150 (MQ=21)

      gCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGatat                                                                                                                                             <  2:1405967/150‑1 (MQ=34)

      gCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGatat                                                                                                                                             <  1:5188774/150‑1 (MQ=34)

      gCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGatat                                                                                                                                             >  1:1005416/1‑150 (MQ=34)

        aCTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTa                                                                                                                                           <  1:3533943/150‑1 (MQ=33)

        aCTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTa                                                                                                                                           <  1:4850601/150‑1 (MQ=33)

        aCTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTa                                                                                                                                           <  1:4850568/150‑1 (MQ=33)

        aCTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTa                                                                                                                                           <  2:60936/150‑1 (MQ=33)

        aCTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTa                                                                                                                                           >  1:1612572/1‑150 (MQ=33)

         ctctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTaa                                                                                                                                          >  2:3345777/1‑150 (MQ=32)

          tctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTaaa                                                                                                                                         >  1:4498865/1‑150 (MQ=32)

          tctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATACTGGATATTaaa                                                                                                                                         <  1:571241/150‑1 (MQ=17)

           ctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAg                                                                                                                                        >  1:4985421/1‑150 (MQ=32)

           ctTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAg                                                                                                                                        >  1:1052859/1‑150 (MQ=32)

             tATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGa                                                                                                                                      >  1:2053507/1‑150 (MQ=32)

               tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaa                                                                                                                                    <  2:5100827/150‑1 (MQ=32)

               tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaa                                                                                                                                    <  2:5100752/150‑1 (MQ=32)

               tGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaa                                                                                                                                    >  2:4336800/1‑150 (MQ=32)

                gACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaa                                                                                                                                   <  1:4997034/150‑1 (MQ=32)

                 aCAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaaa                                                                                                                                  >  2:317720/1‑150 (MQ=31)

                 aCAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaaa                                                                                                                                  >  2:2955526/1‑150 (MQ=31)

                                                                                                            aTCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAATATCATCACAACTGATGCGATGGGTTGCCAGTAAGATATTGCAGAGATGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAc                                       <  1:1014196/150‑1 (MQ=2)

                                                                                                            aTCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAc                                       <  2:2554589/150‑1 (MQ=11)

                                                                                                            aTCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAc                                       <  1:3851049/150‑1 (MQ=11)

                                                                                                            aTCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAc                                       >  1:880135/1‑150 (MQ=11)

                                                                                                             tCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAcc                                      >  2:1665005/1‑150 (MQ=31)

                                                                                                               tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCa                                    <  1:2154677/150‑1 (MQ=32)

                                                                                                               tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCa                                    >  2:609985/1‑150 (MQ=32)

                                                                                                               tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCa                                    <  2:3732679/150‑1 (MQ=32)

                                                                                                               tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCa                                    <  2:1612524/150‑1 (MQ=32)

                                                                                                               tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATAGTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGGAAAAGTAACCCa                                    >  2:3631255/1‑150 (MQ=12)

                                                                                                                aaTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAg                                   <  2:549580/150‑1 (MQ=32)

                                                                                                                aaTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAg                                   <  2:549542/150‑1 (MQ=32)

                                                                                                                 aTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAgg                                  <  1:4804627/150‑1 (MQ=32)

                                                                                                                 aTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAgg                                  <  1:3668015/150‑1 (MQ=32)

                                                                                                                 aTGAGATTACAGCTATCCCAGAACTTCTTAACATACTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAgg                                  >  2:2381384/1‑150 (MQ=17)

                                                                                                                  tGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAggg                                 <  2:4659532/150‑1 (MQ=32)

                                                                                                                    agaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggc                               >  1:4276631/1‑150 (MQ=32)

                                                                                                                    agaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggc                               <  2:381370/150‑1 (MQ=32)

                                                                                                                    agaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggc                               >  1:1202014/1‑150 (MQ=32)

                                                                                                                    agaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggc                               >  2:2957573/1‑150 (MQ=32)

                                                                                                                    agaTTACAGCTATCCCAGAACTACTTAACATGCTTGAAATTAAAGGAAAAAACATCACAACTGATGCGATGGGTTGCCATAAAGAAATTGCAGATATGATACAAAAAACGGGTGGAGCTTATGATATCACGATAAAAGGAACACAgtgga                               >  2:2709961/1‑149 (MQ=2)

                                                                                                                      aTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggcgg                             >  2:4057154/1‑150 (MQ=32)

                                                                                                                      aTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggcgg                             <  1:4803520/150‑1 (MQ=32)

                                                                                                                       ttACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggcggc                            <  2:3454418/150‑1 (MQ=32)

                                                                                                                       ttACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggcggc                            <  2:3454475/150‑1 (MQ=32)

                                                                                                                       ttACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggcggc                            <  2:2639780/150‑1 (MQ=32)

                                                                                                                       ttACAGCTATCCCAGAACTTATTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGggcggc                            <  2:3241542/150‑1 (MQ=17)

                                                                                                                        tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCt                           <  1:2690098/150‑1 (MQ=32)

                                                                                                                        tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCt                           <  1:3184843/150‑1 (MQ=32)

                                                                                                                        tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCt                           <  2:2997849/150‑1 (MQ=32)

                                                                                                                        tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCt                           <  1:2690081/150‑1 (MQ=32)

                                                                                                                                tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAg                   >  2:3203523/1‑150 (MQ=32)

                                                                                                                                 cccAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGc                  <  1:2194498/150‑1 (MQ=32)

                                                                                                                                  ccAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGcc                 <  1:3513248/150‑1 (MQ=32)

                                                                                                                                   cAGAACTTCTTAACATACTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCt                <  1:2104620/150‑1 (MQ=17)

                                                                                                                                     gAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATCAAGCCttt              >  2:5118823/1‑150 (MQ=17)

                                                                                                                                     gAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCttt              >  2:2444069/1‑150 (MQ=32)

                                                                                                                                       aCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGa            >  1:938044/1‑150 (MQ=32)

                                                                                                                                        cttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAg           >  2:4411235/1‑150 (MQ=32)

                                                                                                                                        cttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAg           >  2:4665104/1‑150 (MQ=32)

                                                                                                                                        cttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAg           >  2:3189506/1‑150 (MQ=32)

                                                                                                                                        cttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAg           >  2:3189480/1‑150 (MQ=32)

                                                                                                                                         ttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAgg          >  1:4668985/1‑150 (MQ=32)

                                                                                                                                          tcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGa         >  2:3853507/1‑150 (MQ=32)

                                                                                                                                          tcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGa         >  1:2977193/1‑150 (MQ=32)

                                                                                                                                           cttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaa        <  2:784902/150‑1 (MQ=32)

                                                                                                                                           cttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaa        <  2:4443601/150‑1 (MQ=32)

                                                                                                                                           cttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaa        <  2:785099/150‑1 (MQ=32)

                                                                                                                                             tAACATGCTTGATATTAAATGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa      <  1:4038563/150‑1 (MQ=17)

                                                                                                                                             tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa      >  2:4488138/1‑150 (MQ=32)

                                                                                                                                             tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa      <  1:4038683/150‑1 (MQ=32)

                                                                                                                                             tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa      <  1:4231704/150‑1 (MQ=32)

                                                                                                                                             tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa      >  1:4433158/1‑150 (MQ=32)

                                                                                                                                              aaCATACTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaaa     >  2:4124886/1‑150 (MQ=17)

                                                                                                                                               ccATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAACACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAt    <  1:4699262/149‑1 (MQ=17)

                                                                                                                                               aCATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAt    <  1:4699291/150‑1 (MQ=32)

                                                                                                                                               aCATGCTGGATATTAAAGGAAAAATCAACACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAt    <  2:4395943/150‑1 (MQ=17)

                                                                                                                                                cATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAtt   <  1:2576870/150‑1 (MQ=32)

                                                                                                                                                cATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAtt   <  1:538737/150‑1 (MQ=32)

                                                                                                                                                cATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAtt   <  1:538658/150‑1 (MQ=32)

                                                                                                                                                 aTGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAttt  >  2:3178942/1‑150 (MQ=32)

GCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAACCCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTT  >  CP009273/3618073‑3618367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`