Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,636,207 T→A 12.8% T96S (ACT→TCT)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,636,2070TA12.8% 1037.0 / 125.5 349T96S (ACT→TCT) rsmJ16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (146/156);  new base A (29/16);  total (175/174)
Fisher's exact test for biased strand distribution p-value = 5.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 354 total aligned reads displayed.

CTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCACGCT  >  CP009273/3636058‑3636356
                                                                                                                                                     |                                                                                                                                                     
  ccTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTgg                                                                                                                                                     >  1:4754712/1‑150 (MQ=255)
      cAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATc                                                                                                                                                 <  1:109103/150‑1 (MQ=255)
       aaCCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATcc                                                                                                                                                <  1:100347/150‑1 (MQ=255)
         ccAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccac                                                                                                                                              <  2:3970846/150‑1 (MQ=255)
         ccAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccac                                                                                                                                              >  2:1318505/1‑150 (MQ=255)
         ccAGCCGACGATTTCCGCATCCGCATTACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccac                                                                                                                                              <  2:2842615/150‑1 (MQ=255)
           aGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccacca                                                                                                                                            <  1:1473352/150‑1 (MQ=255)
           aGCCGCCGATGTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccacca                                                                                                                                            <  2:1009977/150‑1 (MQ=255)
            gccgccGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCcaccac                                                                                                                                           >  1:5046511/1‑150 (MQ=255)
             ccgccgATGTCCGCATCCGCATAACCCCGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACcaca                                                                                                                                          <  2:2985185/150‑1 (MQ=255)
              cgccgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACAt                                                                                                                                         >  1:2980809/1‑150 (MQ=255)
              cgccgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACAt                                                                                                                                         >  1:2842356/1‑150 (MQ=255)
                ccgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATcc                                                                                                                                       <  2:4296874/150‑1 (MQ=255)
                ccgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATcc                                                                                                                                       <  2:5020207/150‑1 (MQ=255)
                 cgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCg                                                                                                                                      >  2:1665368/1‑150 (MQ=255)
                 cgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCg                                                                                                                                      >  2:3820232/1‑150 (MQ=255)
                       ccgcatccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAaat                                                                                                                                <  1:607981/150‑1 (MQ=255)
                          catccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAaataat                                                                                                                             <  2:3232704/150‑1 (MQ=255)
                          catccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAaataat                                                                                                                             >  2:4210737/1‑150 (MQ=255)
                           atccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATc                                                                                                                            >  2:691898/1‑150 (MQ=255)
                            tccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCg                                                                                                                           <  2:3714956/150‑1 (MQ=255)
                               gcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCt                                                                                                                        >  1:3161210/1‑150 (MQ=255)
                                catAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCtt                                                                                                                       <  2:2538220/150‑1 (MQ=255)
                                 atAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCttt                                                                                                                      <  2:4634046/150‑1 (MQ=255)
                                  tAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTa                                                                                                                     <  1:1553656/150‑1 (MQ=255)
                                  tAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTa                                                                                                                     >  1:3672880/1‑150 (MQ=255)
                                  tAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTa                                                                                                                     <  1:1304384/150‑1 (MQ=255)
                                    aCCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAAt                                                                                                                   <  2:2078641/150‑1 (MQ=255)
                                        cgcgCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGccc                                                                                                               <  1:4385947/150‑1 (MQ=255)
                                           gcCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCAcc                                                                                                            >  1:1387017/1‑150 (MQ=255)
                                            ccAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCg                                                                                                           <  2:334036/150‑1 (MQ=255)
                                            ccAGGCCATCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCg                                                                                                           >  1:2885710/1‑150 (MQ=255)
                                              aGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCt                                                                                                         <  2:511498/150‑1 (MQ=255)
                                               tgCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCtt                                                                                                        <  2:2722299/149‑1 (MQ=255)
                                               ggCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCtg                                                                                                        >  1:268224/1‑149 (MQ=255)
                                                   gtcgtcGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGc                                                                                                    >  2:790977/1‑150 (MQ=255)
                                                   gtcgtcGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGc                                                                                                    >  1:1661345/1‑150 (MQ=255)
                                                    tcgtcgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGcc                                                                                                   <  1:3371652/150‑1 (MQ=255)
                                                      gtcgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCAc                                                                                                 >  1:1218999/1‑150 (MQ=255)
                                                      gtcgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCAc                                                                                                 <  1:2708321/150‑1 (MQ=255)
                                                       tcgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCAcc                                                                                                <  1:1180229/150‑1 (MQ=255)
                                                       tcgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCAcc                                                                                                <  2:4567213/150‑1 (MQ=255)
                                                        cgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCTTTTAATGCCCACCGCTTTCGCCACCg                                                                                               >  1:3583961/1‑150 (MQ=255)
                                                          aGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGcc                                                                                             <  2:3130218/150‑1 (MQ=255)
                                                           gTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCt                                                                                            <  2:2105716/150‑1 (MQ=255)
                                                             aGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCa                                                                                          >  2:4305726/1‑150 (MQ=255)
                                                                     aaCCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCCCTTTCGCCACCGCCTCACCGCGacc                                                                                  >  2:1304757/1‑150 (MQ=255)
                                                                        cACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGaccacc                                                                               <  2:918199/150‑1 (MQ=255)
                                                                         aCTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCAccg                                                                              >  1:1915294/1‑150 (MQ=255)
                                                                            ggATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCAccgccg                                                                           <  1:1809744/150‑1 (MQ=255)
                                                                                 aCGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGCCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAAttt                                                                      >  1:4879952/1‑150 (MQ=255)
                                                                                  cGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTg                                                                     <  1:409376/150‑1 (MQ=255)
                                                                                   gCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATCATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGc                                                                    >  2:5212740/1‑150 (MQ=255)
                                                                                   gCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGc                                                                    >  2:4732151/1‑150 (MQ=255)
                                                                                    cTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCg                                                                   <  1:1898790/150‑1 (MQ=255)
                                                                                      ccAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTc                                                                 <  2:3784509/150‑1 (MQ=255)
                                                                                        aGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCgg                                                               >  1:3484446/1‑150 (MQ=255)
                                                                                          cATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTg                                                             <  2:4829426/150‑1 (MQ=255)
                                                                                           aTCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTgc                                                            >  1:2145018/1‑150 (MQ=255)
                                                                                           aTCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTgc                                                            >  1:3830092/1‑150 (MQ=255)
                                                                                                cACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCAt                                                       >  1:4151038/1‑150 (MQ=255)
                                                                                                 aCGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATc                                                      >  1:3445272/1‑150 (MQ=255)
                                                                                                  cgcgGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCg                                                     >  1:395473/1‑150 (MQ=255)
                                                                                                     ggCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTGGCGTCGGTGCGCCATCGCTc                                                  <  2:4163735/150‑1 (MQ=255)
                                                                                                      gCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTcc                                                 >  1:2304023/1‑150 (MQ=255)
                                                                                                      gCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTcc                                                 >  1:4430370/1‑150 (MQ=255)
                                                                                                         gCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGcc                                              >  2:152591/1‑150 (MQ=255)
                                                                                                           cGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCcaa                                            <  1:4643399/150‑1 (MQ=255)
                                                                                                            gACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCcaac                                           >  1:703142/1‑150 (MQ=255)
                                                                                                             aCTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGGCGGTGCGCCATCGCTCCGCcaaca                                          >  1:2817406/1‑150 (MQ=255)
                                                                                                               tGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACaaa                                        >  1:241834/1‑150 (MQ=255)
                                                                                                               tGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACaaa                                        >  2:1584711/1‑150 (MQ=255)
                                                                                                                gAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACaaaa                                       >  2:5160570/1‑150 (MQ=255)
                                                                                                                   gCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATca                                    <  2:2356111/150‑1 (MQ=255)
                                                                                                                   gCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATca                                    >  1:1752835/1‑150 (MQ=255)
                                                                                                                   gCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATca                                    <  1:2625247/150‑1 (MQ=255)
                                                                                                                      aGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaaca                                 <  2:2235401/150‑1 (MQ=255)
                                                                                                                      aGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaaca                                 <  2:1956216/150‑1 (MQ=255)
                                                                                                                      aGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaaca                                 <  2:187670/150‑1 (MQ=255)
                                                                                                                      aGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaaca                                 <  2:3511875/150‑1 (MQ=255)
                                                                                                                        tACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACaaa                               <  1:790977/150‑1 (MQ=255)
                                                                                                                         aCAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAg                              <  1:327375/150‑1 (MQ=255)
                                                                                                                            aatgCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATg                           <  2:611455/147‑1 (MQ=255)
                                                                                                                             aGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgc                          <  2:955582/150‑1 (MQ=255)
                                                                                                                             aGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGC‑TTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgcc                         <  2:167256/150‑1 (MQ=255)
                                                                                                                              ggCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgcc                         <  1:3077367/150‑1 (MQ=255)
                                                                                                                              ggCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgcc                         <  2:4028904/150‑1 (MQ=255)
                                                                                                                                cATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgccgc                       >  1:4348283/1‑150 (MQ=255)
                                                                                                                                     cgcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGt                  <  1:4253118/150‑1 (MQ=255)
                                                                                                                                     cgcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGt                  >  1:4978431/1‑150 (MQ=255)
                                                                                                                                     cgcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGt                  <  1:2538130/150‑1 (MQ=255)
                                                                                                                                       cccAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCTCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGttt                <  2:1116497/150‑1 (MQ=255)
                                                                                                                                        ccAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGtttt               >  1:4201362/1‑150 (MQ=255)
                                                                                                                                          aaGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTgg             <  2:2606631/150‑1 (MQ=255)
                                                                                                                                           aGTCCTGCAGTGGCATCCACCACATCCTGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGc            <  2:2890684/150‑1 (MQ=255)
                                                                                                                                            gTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCt           <  2:180714/150‑1 (MQ=255)
                                                                                                                                             tCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtc          >  1:4542177/1‑150 (MQ=255)
                                                                                                                                              ccTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtca         <  2:4855174/150‑1 (MQ=255)
                                                                                                                                                  cAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCAc     <  1:494860/150‑1 (MQ=255)
                                                                                                                                                   aGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCACg    <  1:2070866/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCACGCT  >  CP009273/3636058‑3636356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑