Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,681,219 C→A 21.6% A439S (GCG→TCG)  bcsC ← cellulose synthase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,681,2190CA21.6% 712.4 / 163.0 287A439S (GCG→TCG) bcsCcellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base C (115/109);  new base A (29/34);  total (144/143)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

CAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGCCCAGCCCC  >  CP009273/3681070‑3681367
                                                                                                                                                     |                                                                                                                                                    
  gTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCtt                                                                                                                                                    >  1:2680685/1‑150 (MQ=255)
   tCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTc                                                                                                                                                   <  2:619279/150‑1 (MQ=255)
     gTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAg                                                                                                                                                 <  2:2321520/150‑1 (MQ=255)
     gTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAg                                                                                                                                                 >  1:3843112/1‑150 (MQ=255)
      ttGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGc                                                                                                                                                >  1:4564233/1‑150 (MQ=255)
          cGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCtttt                                                                                                                                            <  1:3668167/150‑1 (MQ=255)
           gCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCttttt                                                                                                                                           <  2:4551703/150‑1 (MQ=255)
           gCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCttttt                                                                                                                                           <  2:1007521/150‑1 (MQ=255)
              gAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTg                                                                                                                                        >  2:1425139/1‑150 (MQ=255)
                 gTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCg                                                                                                                                     <  1:1877338/150‑1 (MQ=255)
                    ctgcCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGAtt                                                                                                                                  >  1:3360187/1‑150 (MQ=255)
                    ctgcCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGAtt                                                                                                                                  >  1:3360125/1‑150 (MQ=255)
                       ccgccTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCt                                                                                                                               <  1:3906623/150‑1 (MQ=255)
                         gccTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTgg                                                                                                                             >  2:441473/1‑150 (MQ=255)
                         gccTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTgg                                                                                                                             >  1:2284821/1‑150 (MQ=255)
                         gccTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTgg                                                                                                                             >  2:1083685/1‑150 (MQ=255)
                           cTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCg                                                                                                                           <  2:1343008/150‑1 (MQ=255)
                            tGCGCCCATTTGCCCTGGTTTTACAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCgg                                                                                                                          >  2:4503925/1‑150 (MQ=255)
                                 ccATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAat                                                                                                                     >  1:587897/1‑150 (MQ=255)
                                 ccATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAat                                                                                                                     <  2:4590734/150‑1 (MQ=255)
                                 ccATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAat                                                                                                                     >  2:2647583/1‑150 (MQ=255)
                                  cATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGGCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAata                                                                                                                    >  1:3128958/1‑150 (MQ=255)
                                  cATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAata                                                                                                                    <  2:371969/150‑1 (MQ=255)
                                   aTTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAatat                                                                                                                   >  2:603896/1‑150 (MQ=255)
                                    tttGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATAtt                                                                                                                  >  1:2853450/1‑150 (MQ=255)
                                       gCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGc                                                                                                               >  1:2637169/1‑150 (MQ=255)
                                        cccTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGcc                                                                                                              >  2:400679/1‑150 (MQ=255)
                                         ccTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCa                                                                                                             <  2:1966516/150‑1 (MQ=255)
                                          cTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAg                                                                                                            <  1:2432416/150‑1 (MQ=255)
                                          cTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGAGATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAg                                                                                                            <  2:3101548/150‑1 (MQ=255)
                                             gTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGccc                                                                                                         >  2:4174973/1‑150 (MQ=255)
                                              ttttCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCcg                                                                                                        <  1:690542/150‑1 (MQ=255)
                                                      tGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATAGTTGCCAGCCCGCGCACGGc                                                                                                >  2:4816686/1‑150 (MQ=255)
                                                         ctctGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGtg                                                                                             >  2:4340536/1‑149 (MQ=255)
                                                          tctGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTa                                                                                            <  2:510508/150‑1 (MQ=255)
                                                            tgcctgcTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAgt                                                                                          <  1:3456608/150‑1 (MQ=255)
                                                                  ctgTGCCAGACGGTCGTTTTTCAGGCTGCGTTAGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTTCTGGCGGTAAATATTTGACAGCCCGCGCACGGCGGTAGTGGTGcc                                                                                    >  1:45219/1‑150 (MQ=255)
                                                                  ctgTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGcc                                                                                    >  1:1344916/1‑150 (MQ=255)
                                                                     tgCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGTCTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCt                                                                                 <  2:2382029/150‑1 (MQ=255)
                                                                      gCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTg                                                                                <  2:1616457/150‑1 (MQ=255)
                                                                        cAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTc                                                                              >  2:1590986/1‑150 (MQ=255)
                                                                        cAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTc                                                                              >  2:4427311/1‑150 (MQ=255)
                                                                          gACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCa                                                                            >  1:4231461/1‑150 (MQ=255)
                                                                            cGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATa                                                                          >  2:1220884/1‑150 (MQ=255)
                                                                             ggTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATAc                                                                         <  1:4704080/150‑1 (MQ=255)
                                                                             ggTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATAc                                                                         <  2:533617/150‑1 (MQ=255)
                                                                             ggTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATAc                                                                         <  2:2225073/150‑1 (MQ=255)
                                                                             ggTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATAc                                                                         <  2:779683/150‑1 (MQ=255)
                                                                              gTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACg                                                                        >  1:1247084/1‑150 (MQ=255)
                                                                              gTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACg                                                                        >  2:2704268/1‑150 (MQ=255)
                                                                                cGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCa                                                                      >  2:1612271/1‑150 (MQ=255)
                                                                                 gTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCaa                                                                     >  1:5009374/1‑150 (MQ=255)
                                                                                   tttGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAgg                                                                   <  1:146295/150‑1 (MQ=255)
                                                                                          gCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTctgctg                                                            >  1:2065646/1‑150 (MQ=255)
                                                                                                ttCGATATCATCAATGCTACGCCGCTGACTGGCAGCGAGCGAGGCGATAAACGATGCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGataata                                                      <  2:719462/150‑1 (MQ=255)
                                                                                                ttCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGataata                                                      >  1:213076/1‑150 (MQ=255)
                                                                                                 tCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGAtaataa                                                     <  1:511701/150‑1 (MQ=255)
                                                                                                 tCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGAtaataa                                                     <  2:1172861/150‑1 (MQ=255)
                                                                                                 tCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGAtaataa                                                     >  1:250126/1‑150 (MQ=255)
                                                                                                  cGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAAc                                                    <  2:157537/150‑1 (MQ=255)
                                                                                                    atatCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGt                                                  >  1:4033245/1‑150 (MQ=255)
                                                                                                     tatCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGtt                                                 >  2:4563892/1‑150 (MQ=255)
                                                                                                      atcatcAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTAGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTc                                                >  2:4224322/1‑150 (MQ=255)
                                                                                                       tcatcaATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTcg                                               >  1:1685721/1‑150 (MQ=255)
                                                                                                         atcaATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTcggc                                             <  2:1312233/150‑1 (MQ=255)
                                                                                                         atcaATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTcggc                                             >  1:4445965/1‑150 (MQ=255)
                                                                                                          tcaATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATCACGTTcggcg                                            >  2:3010550/1‑150 (MQ=255)
                                                                                                           caATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTcggcgg                                           >  1:1166749/1‑150 (MQ=255)
                                                                                                           caATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTcggcgg                                           <  1:1095211/150‑1 (MQ=255)
                                                                                                                 tACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGAt                                     <  1:2212670/150‑1 (MQ=255)
                                                                                                                  aCGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATa                                    <  2:472657/150‑1 (MQ=255)
                                                                                                                  aCGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATa                                    >  2:90468/1‑150 (MQ=255)
                                                                                                                  aCGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATa                                    <  1:4668086/150‑1 (MQ=255)
                                                                                                                   cgccgcTGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATaa                                   >  2:5172625/1‑150 (MQ=255)
                                                                                                                      cgcTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCt                                >  1:4582837/1‑150 (MQ=255)
                                                                                                                      cgcTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCt                                >  2:4150221/1‑150 (MQ=255)
                                                                                                                        cTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCttt                              <  2:2876469/150‑1 (MQ=255)
                                                                                                                         tGACTGGCAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCttnt                             <  1:1622997/150‑1 (MQ=255)
                                                                                                                          gACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTc                            <  1:1009373/150‑1 (MQ=255)
                                                                                                                          gACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTc                            >  2:3446948/1‑150 (MQ=255)
                                                                                                                           aCTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTcg                           <  1:853249/150‑1 (MQ=255)
                                                                                                                                cAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCa                      <  2:2680685/150‑1 (MQ=255)
                                                                                                                                cAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCa                      <  1:2970211/150‑1 (MQ=255)
                                                                                                                                cAGAGAGCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCa                      >  1:4865223/1‑150 (MQ=255)
                                                                                                                                  gagagCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCAGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTTCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATc                    >  1:2120183/1‑150 (MQ=255)
                                                                                                                                   agagCGAGGCGATCAACGATGCAGCATTATCGGGCGATTGCTGGCGGTAAATCTCTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCg                   <  2:3220794/150‑1 (MQ=255)
                                                                                                                                   agagCGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCg                   >  1:1846787/1‑150 (MQ=255)
                                                                                                                                     agCGAGGCGATAAACGCTTCAGCTGGTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGcc                 >  1:1606632/1‑150 (MQ=255)
                                                                                                                                       cGAGGCGATAAACGATTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCcac               >  2:3887023/1‑150 (MQ=255)
                                                                                                                                         aGGCGATACACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCTCGCCATCGCCACAt             <  1:1035636/150‑1 (MQ=255)
                                                                                                                                         aGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACAt             <  1:3513609/150‑1 (MQ=255)
                                                                                                                                         aGGCGATAAACGCTTCAGCTTTTTCAGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACAt             >  2:3538757/1‑150 (MQ=255)
                                                                                                                                          ggCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATc            >  2:1691600/1‑150 (MQ=255)
                                                                                                                                            cGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGc          >  1:4741809/1‑150 (MQ=255)
                                                                                                                                             gATAAACTCTTCATCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGGCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGcc         <  2:3407957/150‑1 (MQ=255)
                                                                                                                                              aTAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGccc        >  2:4965999/1‑150 (MQ=255)
                                                                                                                                               tAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGccca       >  1:783571/1‑150 (MQ=255)
                                                                                                                                                   cGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGcccagccc   >  2:283555/1‑150 (MQ=255)
                                                                                                                                                    gCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGcccagcccc  <  1:400679/150‑1 (MQ=255)
                                                                                                                                                    gCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGcccagcccc  <  1:3037115/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGTCGTTGCCGCTGAAGTGCTGCCGCCTGCGCCCATTTGCCCTGGTTTTCCAGTGCCTCTGCCTGCTGTGCCAGACGGTCGTTTTGCAGGCTGCGTTCGATATCATCAATGCTACGCCGCTGACTGGCAGAGAGCGAGGCGATAAACGCTTCAGCTTTTTCTGGCGATTGCTGGCGGTAAATATTTGCCAGCCCGCGCACGGCGTTAGTGTTGCCGCTGTCCATACGCAAGGTCTGCTGATAATAACGTTCGGCGGCGGGATAATCTTTTCGCGCCATCGCCACATCGCCCAGCCCC  >  CP009273/3681070‑3681367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑