Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,469 A→C 6.4% D421E (GAT→GAG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4690AC 6.4% 594.0 / 6.1 246D421E (GAT→GAG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base A (82/136);  new base C (15/8);  total (102/144)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC  >  CP009273/3721321‑3721618
                                                                                                                                                    |                                                                                                                                                      
tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAt                                                                                                                                                       <  2:2015885/150‑1 (MQ=255)
tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAt                                                                                                                                                       <  1:501148/150‑1 (MQ=255)
    tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑cccc                                                                                                                                                  >  2:1545039/1‑150 (MQ=255)
       tcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccccc                                                                                                                                               <  1:4497463/150‑1 (MQ=255)
        cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGG‑cgcccccc                                                                                                                                              >  1:4615415/1‑150 (MQ=255)
        cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑ggcccccc                                                                                                                                              >  2:465907/1‑150 (MQ=255)
        cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑cccccccc                                                                                                                                              >  1:2012557/1‑150 (MQ=255)
        cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑cccccccc                                                                                                                                              >  1:3049483/1‑150 (MQ=255)
         gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCg                                                                                                                                             >  1:3333735/1‑150 (MQ=255)
         gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCg                                                                                                                                             >  2:470723/1‑150 (MQ=255)
         gtGGCTGAGAAGCGGCATAACCCTGCGCATCGGGTATATGCGACTGTGCTAACGGTAGTTGCGGCAACAATTCACTGAGCGTTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTTCTGGCCCCACAT‑CCCCCCCCg                                                                                                                                             <  2:1113252/150‑1 (MQ=255)
            gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGTTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTAc                                                                                                                                          <  2:4357177/150‑1 (MQ=255)
            gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTAc                                                                                                                                          <  1:5170636/150‑1 (MQ=255)
            gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTAc                                                                                                                                          <  2:4357714/150‑1 (MQ=255)
              tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCgg                                                                                                                                        >  1:2262567/1‑150 (MQ=255)
              tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACgg                                                                                                                                        >  1:2262896/1‑150 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCTACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCCCCCCGTACggg                                                                                                                                       >  2:4032249/1‑149 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CGCCCCCcggtcggt                                                                                                                                       >  2:4488804/1‑150 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGt                                                                                                                                       >  2:4032410/1‑150 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCGCGTGCggg                                                                                                                                       >  1:684324/1‑149 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGGTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGt                                                                                                                                       <  2:707223/150‑1 (MQ=255)
                 tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑CCCCCCCCGGACgggtc                                                                                                                                     >  1:3898857/1‑147 (MQ=255)
                      ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CGCCCCCCGTCCGGtcggcgag                                                                                                                                >  1:3523433/1‑143 (MQ=255)
                         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                                             <  1:4344021/150‑1 (MQ=255)
                         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                                             <  1:1736468/150‑1 (MQ=255)
                           aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                                           <  1:4207439/150‑1 (MQ=255)
                             cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGGATCGAGCTGCTGa                                                                                                                         >  1:2673432/1‑150 (MQ=255)
                               cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGAcg                                                                                                                       >  2:3224004/1‑149 (MQ=255)
                                 gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCCCCGGGCGGTATGCGAGCGGCTGGCCGc                                                                                                                     >  2:3477014/1‑150 (MQ=255)
                                  cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                    <  2:5230844/150‑1 (MQ=255)
                                      tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                <  1:744447/150‑1 (MQ=255)
                                         ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                             <  2:2134644/150‑1 (MQ=255)
                                         ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGACGGGGCTCGGGCTGCTGGCCGCTGTTGTcc                                                                                                             >  1:3271132/1‑150 (MQ=255)
                                            aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                                          <  1:4768370/150‑1 (MQ=255)
                                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                        <  2:1849207/150‑1 (MQ=255)
                                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                        <  2:3843429/150‑1 (MQ=255)
                                               tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                        <  1:5056572/150‑1 (MQ=255)
                                                 cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                     <  1:5110903/150‑1 (MQ=255)
                                                  gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                    <  1:1350175/150‑1 (MQ=255)
                                                  gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                    <  1:4219310/150‑1 (MQ=255)
                                                   aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                   <  1:2751983/150‑1 (MQ=255)
                                                     tGTTCTAACGGTATTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                 <  1:2888493/150‑1 (MQ=255)
                                                     tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                 <  1:413729/150‑1 (MQ=255)
                                                     tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGCTCTCCGCCCGCGTCGGa                                                                                                 >  2:3078691/1‑150 (MQ=255)
                                                      gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                <  2:3475604/150‑1 (MQ=255)
                                                       ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                               <  2:1401651/150‑1 (MQ=255)
                                                       ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                               <  1:3370112/150‑1 (MQ=255)
                                                       ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                               <  1:3370060/150‑1 (MQ=255)
                                                         cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                             <  1:3821436/150‑1 (MQ=255)
                                                         cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                             <  1:420214/150‑1 (MQ=255)
                                                            aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                                          <  2:2480550/150‑1 (MQ=255)
                                                            aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTCATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGt                                                                                          >  2:3896558/1‑150 (MQ=255)
                                                            aCGGTAGTTGCGGCAACAAGTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                                          <  2:5072219/150‑1 (MQ=255)
                                                              ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑CCCCCCGCGTTCGGTCAGCGAGCTGCTGACCGCTGAGATGCGCCCGCAGCGGACGCCGGtgc                                                                                        >  2:3687263/1‑148 (MQ=255)
                                                              ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                                        <  1:3499413/150‑1 (MQ=255)
                                                                          aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑GCCCCCCCGTACGGTAATCGAGCTGCGGCCCGGTGATCTGCGCCAGCTTGGGGGGCCAGGACTCGCTAGGggcc                                                                            >  2:5204134/1‑150 (MQ=255)
                                                                          aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                            >  2:416490/1‑150 (MQ=255)
                                                                            caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCTTCGGACGCCCGTACTCACTACGcgcccc                                                                          >  2:3562936/1‑150 (MQ=255)
                                                                               ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGCCGGGCATCGGGCTGCGGGCCGCGGGGAGCCGCCCGCATCTGCCGCCAGTCCGCGCGGCGggccccgcc                                                                       >  1:3648441/1‑150 (MQ=255)
                                                                                gcactGAGCGAATTCTCTGGCTGCGCCGCGAACTGCGCCAGCCTTGCGGCGCCCAGTGCTGGCCCCACCC‑CCCCCCCCGTCCGGGCCACGAGCGGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACGCACCACGcgcccccccc                                                                      <  2:3181438/146‑1 (MQ=255)
                                                                                 cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                     <  1:2567913/150‑1 (MQ=255)
                                                                                 cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                     <  1:436330/150‑1 (MQ=255)
                                                                                  aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑GCCCCCCCGTACGGTGATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                    >  1:1116327/1‑150 (MQ=255)
                                                                                   aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                                   <  1:2157652/150‑1 (MQ=255)
                                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTAATCGAGCTGCTGAGCGCTGGTATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATc                                                                >  1:1181865/1‑150 (MQ=255)
                                                                                       gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                               <  1:4292234/150‑1 (MQ=255)
                                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                             <  1:1430492/150‑1 (MQ=255)
                                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                             <  1:1492912/150‑1 (MQ=255)
                                                                                          aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCCGCATCTGACGCCAGTCCTCCCTACGCGCCCCGCCCCCAAGCAACg                                                            >  2:3335220/1‑150 (MQ=255)
                                                                                          aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGGCGCCCGTACTCACTACGCGCCCCGCGCCCCATCAACg                                                            >  1:4526845/1‑150 (MQ=255)
                                                                                           tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCGGGTATCCGCCAGCCTCTGTCGCCAGTACTCGCTACGCGCCCCGCCCCCAATCAACgg                                                           >  1:1040507/1‑149 (MQ=255)
                                                                                                ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTTAACGAGATGCTGGCCGCGGAGATACGCCAGCTTCTGACGCCTGCACTCTCTACGCGCGCCGCACCCACTAAACAGAacaa                                                      >  2:2824975/1‑149 (MQ=255)
                                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAAGCGAGCTGCTGACCGCGGGTGTCCGCCAGCATCTGACGCCGGTACTCACTCCGCGCCCCGCCCCCAAGCAACGGGACACt                                                     >  1:4118856/1‑150 (MQ=255)
                                                                                                    aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTGATCGAGCTGCTGGCCGCTGTTATCCGCCCGCATCTGGCGCCCGTACTCGCTACGCGCCCCGCCCCCAATCAACGTAAGACTTTg                                                  >  1:3207465/1‑150 (MQ=255)
                                                                                                    aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                  <  1:2498545/150‑1 (MQ=255)
                                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt                                              <  1:4992003/150‑1 (MQ=255)
                                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt                                              <  1:3730646/150‑1 (MQ=255)
                                                                                                        gccgcGATCTGCGCCAGACTTGCTGCGACCAGTGCAGGCCCCACAA‑CCCCCCCCGAACGGTCATCGAGCTGCTGACCGCGGATATCCTCAGGCATCTGACTCCAGAACTCACTACGCGCCCCGCCCCCAAGAACCGTACCACTTTGCggg                                              >  2:2322715/1‑149 (MQ=255)
                                                                                                         ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTGTGCGGtg                                             >  2:3021924/1‑149 (MQ=255)
                                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                            <  1:2081977/150‑1 (MQ=255)
                                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                            >  2:4889347/1‑150 (MQ=255)
                                                                                                           gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                           <  1:3141416/150‑1 (MQ=255)
                                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGAGCGCTGATTTGCGCCAGCATCTGACGCCAGGACTCACTTCGCGCCCCGCCCCCAATCAACGTTACACTTTGCGGTGTAAt                                         >  1:2166145/1‑150 (MQ=255)
                                                                                                             gATATGAGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCACTTACGGTAATCGAGATGCTGACCGCTGATATCCGCCAGCTTCTGACTCAAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                         <  2:1597869/150‑1 (MQ=255)
                                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGGTCGCCCCACAT‑CCCCCCCCGGACGGTAATCGATCTGCTGACCGCTGATATCCGCAATCATCTGACGCCAGAACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                     <  2:4111305/150‑1 (MQ=255)
                                                                                                                      cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCAGCTGTCCGCTGATATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTGAATACCGCAGGc                                >  2:1619055/1‑150 (MQ=255)
                                                                                                                      cAGCCTCGCTGCGCCCAGCGCGGGCCCCCCCT‑CCCCCCCCGTACGCTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                                <  2:3008902/150‑1 (MQ=255)
                                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCCATCCACGTGACACGTTGCGGGTTAATACAGCAGGcgg                              >  2:5015667/1‑148 (MQ=255)
                                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                             <  1:2518074/150‑1 (MQ=255)
                                                                                                                          cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACACACAACCCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTGAAAACCGCAGGCATGc                            >  2:2785920/1‑150 (MQ=255)
                                                                                                                           ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                           <  1:4027532/150‑1 (MQ=255)
                                                                                                                           ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                           <  1:862530/150‑1 (MQ=255)
                                                                                                                               tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc                       <  1:951272/150‑1 (MQ=255)
                                                                                                                                gccccccGTCCTGGCCCCACAC‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCACTCAAACTAACCCTTTGCGGTTTAATACCGCAGGCAAGCACGACa                      <  2:1431847/147‑1 (MQ=255)
                                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGCTAATCGAGCTGCGGCCCGCTGATATCCGCCAGCATCAGTCGCCAGTCCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTTCGCTTTAAGACCGCAGGTATGCACGACac                     <  2:1288990/150‑2 (MQ=255)
                                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCCTGCACGACAt                     >  1:2540479/1‑150 (MQ=255)
                                                                                                                                   cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                   <  1:1530789/150‑1 (MQ=255)
                                                                                                                                        tGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACTGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc              <  1:4148502/150‑1 (MQ=255)
                                                                                                                                           tGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc           >  2:927365/1‑150 (MQ=255)
                                                                                                                                              ccccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGAACGACATCCATGCCATCTGc        >  2:4536412/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC  >  CP009273/3721321‑3721618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑