Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 8.2% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG 8.2% 569.7 / 6.7 238Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (86/122);  new base G (17/9);  total (103/135)
Fisher's exact test for biased strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721336‑3721634
                                                                                                                                                      |                                                                                                                                                     
gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGt                                                                                                                                                       >  2:4488962/1‑150 (MQ=255)
gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGt                                                                                                                                                       >  2:4032410/1‑150 (MQ=255)
gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGt                                                                                                                                                       >  2:4488545/1‑150 (MQ=255)
     gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCg                                                                                                                                                  <  2:3755936/150‑1 (MQ=255)
       ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CGCCCCCCGTCCGGtcggcgag                                                                                                                                                >  1:3523433/1‑143 (MQ=255)
          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                                                             <  1:4344021/150‑1 (MQ=255)
            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCGCCGTTCGGTGATCGAgctgcg                                                                                                                                           >  1:3704701/1‑149 (MQ=255)
            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                           <  1:4207478/150‑1 (MQ=255)
            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                           <  1:4207439/150‑1 (MQ=255)
                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                                      <  2:181857/150‑1 (MQ=255)
                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑AGCCCGCCGTGCGGGGAGCGAGCGGCTGGCCg                                                                                                                                      >  2:321214/1‑150 (MQ=255)
                  gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCGCCCGTCCGGTAATCGTGctggtgtgcgc                                                                                                                                     >  2:3476977/1‑145 (MQ=255)
                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                                    <  2:5230844/150‑1 (MQ=255)
                       tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                                <  1:690456/150‑1 (MQ=255)
                        cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                               <  2:1096040/150‑1 (MQ=255)
                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                             <  2:2134644/150‑1 (MQ=255)
                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGACGGGGCTCGGGCTGCTGGCCGCTGTTGTcc                                                                                                                             >  1:3271132/1‑150 (MQ=255)
                               aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                        <  2:3843429/150‑1 (MQ=255)
                                tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                        <  1:5056572/150‑1 (MQ=255)
                                   gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                    <  1:1350175/150‑1 (MQ=255)
                                   gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCGGATATCGGCCCGCagc                                                                                                                    >  2:5033302/1‑148 (MQ=255)
                                   gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                    <  1:4219310/150‑1 (MQ=255)
                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                   <  1:2751983/150‑1 (MQ=255)
                                     cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTg                                                                                                                  >  2:1598601/1‑150 (MQ=255)
                                      tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGGCGGTAAGCGGGCTGCTGTCCGCTGGTGTCCGCCAGCGTCTgg                                                                                                                 >  1:2569363/1‑149 (MQ=255)
                                      tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                 <  1:413729/150‑1 (MQ=255)
                                      tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGCTCTCCGCCCGCGTCGGa                                                                                                                 >  2:3078691/1‑150 (MQ=255)
                                      cggtctcACGCTAGTTGCGGCAACAACTCACTGAGCGATTCCTCGGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCCGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                 <  2:4867585/147‑1 (MQ=255)
                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  1:1220316/150‑1 (MQ=255)
                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  1:3370060/150‑1 (MQ=255)
                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  1:3370112/150‑1 (MQ=255)
                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  1:112910/150‑1 (MQ=255)
                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  2:1401651/150‑1 (MQ=255)
                                          cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                             <  1:3821311/150‑1 (MQ=255)
                                             aCGGTAGTTGCGGCAACAAGTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                                                          <  2:5072219/150‑1 (MQ=255)
                                                         gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CGCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGTTAGCCGCCAGCGTCTGACGCCAGTACTCACTAcgcg                                                                                              >  1:4149925/1‑150 (MQ=255)
                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGGTGATCTCCGCCAGCATCTGACGCCCGTACTCACGACgcgc                                                                                             >  1:3840938/1‑150 (MQ=255)
                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGGACTCGAGGTGCTGTCCGCTGTTGTCCGCCAGCATCGGACGCCAGGACTCGCTACgcgc                                                                                             >  2:872853/1‑150 (MQ=255)
                                                           aacaaTTCCATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCCGCGCCCAGGGCTGGCCCCACCT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                                            <  2:4668253/150‑1 (MQ=255)
                                                           aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                                            >  2:416490/1‑150 (MQ=255)
                                                             caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCTCAT‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                          <  2:5205017/150‑1 (MQ=255)
                                                              aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGAATCGAGCTGCTGGCCGCTGATGTCCGCCAGCGGCTGGCGCCAGTACTCGCGACGcgccccg                                                                                         >  1:896211/1‑150 (MQ=255)
                                                               aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTCCTCACTACGcgccccgc                                                                                        >  2:2979070/1‑150 (MQ=255)
                                                                 tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGTGCTGCTGACCGCTGTTGTCCGCCAGCGTCTGCCGCGGGGGCTCACTAGGcgccccgccg                                                                                      >  1:5128724/1‑149 (MQ=255)
                                                                 gcactGAGCGAATTCTCTGGCTGCGCCGCGAACTGCGCCAGCCTTGCGGCGCCCAGTGCTGGCCCCACCC‑CCCCCCCCGTCCGGGCCACGAGCGGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACGCACCACGcgcccccccc                                                                                      <  2:3181438/146‑1 (MQ=255)
                                                                  cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                     <  1:436330/150‑1 (MQ=255)
                                                                   ccggAGCGACTTCACAGGATGCGCCGCGATCTGCGCCAGCCTCGCTGCGCCCCGTGCCGGCCCCACAG‑CCCCCCCCGTACGCCAATCCAGCTGCGGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                    <  2:356098/147‑1 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑GCCCCCCCGTACGGTGATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                    >  1:1116327/1‑150 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGGAATCGAGCTGCTGGCCGCTGTGATCCGCCAGCATCGGACGCCCGTGCTCACGACGCGCCCCGccccc                                                                                    >  1:4199863/1‑150 (MQ=255)
                                                                    aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTACTCGAGCTGCTGGCCGCTGCTATCCGCCCGCATCTGCCGCCGGGACTCACGACGCGCCCCGCCCCCa                                                                                   >  2:1922647/1‑150 (MQ=255)
                                                                    aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                                                   <  1:2157364/150‑1 (MQ=255)
                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATAGCCGCCAGCATCTGACGCCAGTCCTCACTACGCGCCCCGcccccccgc                                                                                >  1:2929462/1‑146 (MQ=255)
                                                                        gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                                               <  1:4292234/150‑1 (MQ=255)
                                                                           aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCCGCATCTGACGCCAGTCCTCCCTACGCGCCCCGCCCCCAAGCAACg                                                                            >  2:3335220/1‑150 (MQ=255)
                                                                            tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCGGGTATCCGCCAGCCTCTGTCGCCAGTACTCGCTACGCGCCCCGCCCCCAATCAACgg                                                                           >  1:1040507/1‑149 (MQ=255)
                                                                              tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                         <  1:470723/150‑1 (MQ=255)
                                                                               ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTTCGGTGCTCGAGCTGCGGAGCGCGGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCGCCGCCCCCACTCAACGTAac                                                                        >  1:4514486/1‑150 (MQ=255)
                                                                               ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGGTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGGACTCCCGACGCGCCCCGGCCCCCATCCACGTAac                                                                        >  1:1785163/1‑150 (MQ=255)
                                                                                  tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTTATCGAGCTGCTGACCGCGGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAacacg                                                                     >  1:1510211/1‑149 (MQ=255)
                                                                                    gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                                   <  1:1728851/150‑1 (MQ=255)
                                                                                     aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGGCCGCGGATATCCGCCAGCATGTGACGCCAGTACTCACTGCGCGCCCCGCCGCCACTCGACGTAACACGTTg                                                                  >  1:3587443/1‑150 (MQ=255)
                                                                                         gccgcGATCTGCGCCAGACTTGCTGCGACCAGTGCAGGCCCCACAA‑CCCCCCCCGAACGGTCATCGAGCTGCTGACCGCGGATATCCTCAGGCATCTGACTCCAGAACTCACTACGCGCCCCGCCCCCAAGAACCGTACCACTTTGCggg                                                              >  2:2322715/1‑149 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGTTATCCGCCAGCATCTGGCGCCAGTTCTCACTACGCGCCCCGCCCCCCATCAACGTAGCACGTTGCgggt                                                             >  1:3476608/1‑148 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTGTGCGGtg                                                             >  2:3021924/1‑149 (MQ=255)
                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                            <  1:2081977/150‑1 (MQ=255)
                                                                                            gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                           <  1:3141409/150‑1 (MQ=255)
                                                                                            gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                           <  1:3141665/150‑1 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:4981416/150‑1 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:857537/150‑1 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:1471207/150‑1 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGAGCGCTGATTTGCGCCAGCATCTGACGCCAGGACTCACTTCGCGCCCCGCCCCCAATCAACGTTACACTTTGCGGTGTAAt                                                         >  1:2166145/1‑150 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCAGACGAGAGTACTCACTACGCGCCCCGCCCCCAATCACCGTAACAGGTTGCGGTTTAAt                                                         <  2:1597589/150‑1 (MQ=255)
                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGGTCGCCCCACAT‑CCCCCCCCGGACGGTAATCGATCTGCTGACCGCTGATATCCGCAATCATCTGACGCCAGAACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                                     <  2:4111305/150‑1 (MQ=255)
                                                                                                      ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGGTTTAATACCGCAgg                                                 >  1:3215380/1‑150 (MQ=255)
                                                                                                       cAGCCGTGCTGCGCCCAGTGCCGGCCCCCCCT‑CCCCCCCCGTACGGTCCTCGCGCTGCCGACCGCCGAGCTCCGCCAGCTTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCCCCGTAACACTTTGCGCTTTAAGACCGCAGGc                                                <  2:3009235/150‑1 (MQ=255)
                                                                                                        aGCTTTGCTGCTCCGATTTCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTTCTGTCCGCTTTTTTGCGCCTGCATCTTTCGCCAGTACTCTCTACGCGCCCCGCCCCCAATGAACGTTTCTCTTTGCTGTTTAATAACGCAGGCa                                               <  2:3961773/150‑1 (MQ=255)
                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                             <  1:2518074/150‑1 (MQ=255)
                                                                                                           cgtgctgcGCCCTGTGCATGCCCCACAC‑CCCCCCCCGTACGGTTCTCGCGCTGCTGACCGCGGATATCCGCCAGCATCTGACTCCAGTACTAACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGTTTTAATACCGCAGGCATGc                                            <  2:3589985/148‑1 (MQ=255)
                                                                                                           cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACACACAACCCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTGAAAACCGCAGGCATGc                                            >  2:2785920/1‑150 (MQ=255)
                                                                                                            ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                           <  1:4027532/150‑1 (MQ=255)
                                                                                                             ggctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCATTACCCACTACGCGCCCCGCCCCCAATCCCCGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                          <  2:2599573/149‑1 (MQ=255)
                                                                                                                 gccccccGTCCTGGCCCCACAC‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCACTCAAACTAACCCTTTGCGGTTTAATACCGCAGGCAAGCACGACa                                      <  2:1431847/147‑1 (MQ=255)
                                                                                                                  cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGCTAATCGAGCTGCGGCCCGCTGATATCCGCCAGCATCAGTCGCCAGTCCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTTCGCTTTAAGACCGCAGGTATGCACGACac                                     <  2:1288990/150‑2 (MQ=255)
                                                                                                                  cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCCTGCACGACAt                                     >  1:2540479/1‑150 (MQ=255)
                                                                                                                  cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                     >  1:2246572/1‑150 (MQ=255)
                                                                                                                            tGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                           >  2:927365/1‑150 (MQ=255)
                                                                                                                                cccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                       <  1:5059819/150‑1 (MQ=255)
                                                                                                                                  cacaT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg                     >  1:4796389/1‑150 (MQ=255)
                                                                                                                                  cacaT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATACCATCAGCCAg                     >  1:5004745/1‑150 (MQ=255)
                                                                                                                                    caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                   >  1:2821376/1‑150 (MQ=255)
                                                                                                                                      t‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                 >  1:3068430/1‑150 (MQ=255)
                                                                                                                                        ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt                <  1:454513/150‑1 (MQ=255)
                                                                                                                                          ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACTCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  2:2152785/1‑150 (MQ=255)
                                                                                                                                          ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  2:2158049/1‑150 (MQ=255)
                                                                                                                                                gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg        >  2:3459783/1‑150 (MQ=255)
                                                                                                                                                   cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt     >  1:2427493/1‑150 (MQ=255)
                                                                                                                                                   cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt     >  2:2915145/1‑150 (MQ=255)
                                                                                                                                                   cGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt     <  1:3658093/150‑1 (MQ=255)
                                                                                                                                                     gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc   >  1:5096557/1‑150 (MQ=255)
                                                                                                                                                      tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc  >  2:3318445/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721336‑3721634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑