Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,894 C→A 69.8% M244I (ATG→ATT)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,8940CA69.8% 383.2 / 312.1 305M244I (ATG→ATT) atpGF1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base C (44/48);  new base A (116/97);  total (160/145)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 307 total aligned reads displayed.

TACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCA  >  CP009273/3910747‑3911041
                                                                                                                                                   |                                                                                                                                                   
tACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGccacctt                                                                                                                                                   >  2:5239629/1‑148 (MQ=255)
  cGAAATAACCTGTTTAATCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATAc                                                                                                                                                 <  2:2534047/150‑1 (MQ=255)
  cGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATAc                                                                                                                                                 >  2:4628603/1‑150 (MQ=255)
   gAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACg                                                                                                                                                >  1:4976263/1‑150 (MQ=255)
    aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACgg                                                                                                                                               >  1:2096731/1‑150 (MQ=255)
      aTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggc                                                                                                                                             <  2:2991988/150‑1 (MQ=255)
        aaCCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGATGGCCTGACGAGCTTTGGTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGggcgg                                                                                                                                           >  2:1730481/1‑150 (MQ=255)
         aCCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggcggc                                                                                                                                          >  2:847672/1‑150 (MQ=255)
          ccTGTTTAAACCGCGGCGGCCCCCGAGACGAACTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTGTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTGTCATCGCCACAATACGGGCGGcc                                                                                                                                         >  2:2725093/1‑150 (MQ=255)
            tGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCGGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTGTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTGCATCGCCACAATACGGGCGGCCTg                                                                                                                                       >  2:1470326/1‑150 (MQ=255)
             gTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGc                                                                                                                                      >  2:1399834/1‑150 (MQ=255)
               ttAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTc                                                                                                                                    <  1:1403441/150‑1 (MQ=255)
                tAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCg                                                                                                                                   >  1:4592827/1‑150 (MQ=255)
                 aaaCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGc                                                                                                                                  >  1:3284840/1‑150 (MQ=255)
                 aaaCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGc                                                                                                                                  >  2:2345922/1‑150 (MQ=255)
                   aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTg                                                                                                                                >  2:1812730/1‑150 (MQ=255)
                   aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTg                                                                                                                                <  1:3444871/150‑1 (MQ=255)
                     cgcgGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGc                                                                                                                              >  2:3460522/1‑150 (MQ=255)
                     cgcgGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGc                                                                                                                              >  2:1287572/1‑150 (MQ=255)
                            gcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGttt                                                                                                                       >  2:4882053/1‑150 (MQ=255)
                              ccccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTc                                                                                                                     <  1:4793326/150‑1 (MQ=255)
                              ccccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTc                                                                                                                     >  2:3176941/1‑150 (MQ=255)
                               cccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAgg                                                                                                                          >  2:3939015/1‑144 (MQ=255)
                                ccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCaa                                                                                                                   >  1:277030/1‑150 (MQ=255)
                                  gagaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAAcc                                                                                                                 <  2:4588719/150‑1 (MQ=255)
                                    gaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCAc                                                                                                               <  2:197760/150‑1 (MQ=255)
                                    gaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCAc                                                                                                               <  2:4571777/150‑1 (MQ=255)
                                        aTCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGccc                                                                                                           <  1:4251196/150‑1 (MQ=255)
                                          ctcGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTg                                                                                                         <  1:839509/150‑1 (MQ=255)
                                            cGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGAt                                                                                                       >  1:3131405/1‑150 (MQ=255)
                                              gTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaa                                                                                                     >  2:2876135/1‑150 (MQ=255)
                                                gAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATCAAc                                                                                                   >  2:3638410/1‑150 (MQ=255)
                                                    tCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTga                                                                                               <  1:532900/150‑1 (MQ=255)
                                                       tGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGAt                                                                                            >  1:66029/1‑150 (MQ=255)
                                                         aGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTc                                                                                          <  2:4496826/150‑1 (MQ=255)
                                                          gTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCg                                                                                         >  1:2666512/1‑150 (MQ=255)
                                                           tAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGa                                                                                        <  1:2533930/150‑1 (MQ=255)
                                                            aaTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGAc                                                                                       >  2:4655322/1‑150 (MQ=255)
                                                             aTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACa                                                                                      >  2:3542475/1‑150 (MQ=255)
                                                                 tGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAac                                                                                  >  2:5142276/1‑150 (MQ=255)
                                                                  ggCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCTGCCTGCTCGCTGGACAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAacg                                                                                 >  2:1228292/1‑150 (MQ=255)
                                                                          gAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACgcagca                                                                         <  2:549173/150‑1 (MQ=255)
                                                                             cTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAggg                                                                      >  2:2835458/1‑150 (MQ=255)
                                                                              tttGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGt                                                                     <  1:1667437/150‑1 (MQ=255)
                                                                              tttGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGt                                                                     >  2:270246/1‑150 (MQ=255)
                                                                               ttgttgTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTa                                                                    >  2:5015596/1‑150 (MQ=255)
                                                                                 gttgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATc                                                                  <  1:1989859/150‑1 (MQ=255)
                                                                                  ttgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTTATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATcc                                                                 <  1:766789/150‑1 (MQ=255)
                                                                                   tgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCa                                                                >  2:40063/1‑150 (MQ=255)
                                                                                   tgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATAGCCAAAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGACATTCGACATAACGACGCAGCAGGGTAACCa                                                                >  2:152948/1‑150 (MQ=255)
                                                                                    gtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAg                                                               <  1:4103647/150‑1 (MQ=255)
                                                                                      ataCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCa                                                             <  1:2365943/150‑1 (MQ=255)
                                                                                      ataCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCa                                                             >  2:3399127/1‑150 (MQ=255)
                                                                                      ataCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCa                                                             >  1:2998572/1‑150 (MQ=255)
                                                                                        aCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAAc                                                           >  2:4958417/1‑150 (MQ=255)
                                                                                             cTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCtt                                                      >  2:1617419/1‑150 (MQ=255)
                                                                                             cTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCtt                                                      <  1:449317/150‑1 (MQ=255)
                                                                                               gCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCg                                                    >  1:1504054/1‑150 (MQ=255)
                                                                                                cAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCgg                                                   <  1:2327497/150‑1 (MQ=255)
                                                                                                 aGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGa                                                  >  1:180048/1‑150 (MQ=255)
                                                                                                 aGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGa                                                  <  2:4813025/150‑1 (MQ=255)
                                                                                                   ctctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATc                                                <  1:4416378/150‑1 (MQ=255)
                                                                                                    tctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCg                                               >  2:2569121/1‑150 (MQ=255)
                                                                                                     ctgtAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCGGCTCGCGGGCCAGGTTTTCAACCACGCCCGGATAAACCTGAGATTCGACAGAACGCCGCCGCAGGGTATCCCGCAACGCCTGCGCAGCgg                                              >  2:3118455/4‑150 (MQ=255)
                                                                                                     ctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCgg                                              >  1:1574643/1‑150 (MQ=255)
                                                                                                     ctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCgg                                              >  2:1777245/1‑150 (MQ=255)
                                                                                                        tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGtt                                           <  1:3202723/150‑1 (MQ=255)
                                                                                                        tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGACCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATACCGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGtt                                           >  2:1706565/1‑150 (MQ=255)
                                                                                                         aaTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTc                                          <  1:103161/150‑1 (MQ=255)
                                                                                                         aaTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTc                                          <  2:1168410/150‑1 (MQ=255)
                                                                                                          aTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTCTCCAGCAACGCCTTCGGATCGGGTTCg                                         >  2:3560048/1‑150 (MQ=255)
                                                                                                          aTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCg                                         >  2:473793/1‑150 (MQ=255)
                                                                                                             aGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTAc                                      >  1:3577996/1‑150 (MQ=255)
                                                                                                             aGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTAc                                      >  2:5065478/1‑150 (MQ=255)
                                                                                                                 tGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGt                                  >  2:4143062/1‑150 (MQ=255)
                                                                                                                  gccgccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTa                                 <  1:4697634/150‑1 (MQ=255)
                                                                                                                    cgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAAt                               >  2:2210112/1‑150 (MQ=255)
                                                                                                                     gccATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATc                              <  2:75243/150‑1 (MQ=255)
                                                                                                                     gccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATc                              >  1:3970078/1‑150 (MQ=255)
                                                                                                                     gccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATc                              >  1:3143921/1‑150 (MQ=255)
                                                                                                                      ccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATcc                             >  2:525204/1‑150 (MQ=255)
                                                                                                                          tGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAgg                         <  2:2636906/150‑1 (MQ=255)
                                                                                                                              ggtcgCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt                     >  2:3601393/1‑150 (MQ=255)
                                                                                                                              ggtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt                     <  1:1613897/150‑1 (MQ=255)
                                                                                                                               gtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTa                    <  2:573913/150‑1 (MQ=255)
                                                                                                                                tcgCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTAt                   <  2:3768539/150‑1 (MQ=255)
                                                                                                                                tcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTAt                   <  1:2502534/150‑1 (MQ=255)
                                                                                                                                 cgcgGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATg                  >  2:2064320/1‑150 (MQ=255)
                                                                                                                                 cgcgGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATg                  >  2:2266860/1‑150 (MQ=255)
                                                                                                                                 cgcgGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATg                  <  2:44328/150‑1 (MQ=255)
                                                                                                                                  gcgGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGt                 <  1:3855328/150‑1 (MQ=255)
                                                                                                                                   cgGCTATCATCGCCACAAAACCTGCGGCCAGCTCGCTGGCCAGATTATCAACCACGCCCTGATAAACAAGAGATTCGACAAAACCAACCAGCAGGGTATCCAGCAACGCCTACGCATCTGATTCGTAAACTAAATCACAGTATATATGta                >  2:1592237/1‑149 (MQ=255)
                                                                                                                                    ggCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGttt               >  2:1057425/1‑150 (MQ=255)
                                                                                                                                     gCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTc              >  1:3978077/1‑150 (MQ=255)
                                                                                                                                      cTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCa             <  1:456315/150‑1 (MQ=255)
                                                                                                                                       tttCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAg            <  1:1298150/150‑1 (MQ=255)
                                                                                                                                         tCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGat          <  1:4840871/150‑1 (MQ=255)
                                                                                                                                            tCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcat       <  1:5146456/150‑1 (MQ=255)
                                                                                                                                             cGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGATTTCAACCACGCCCTGATAAACCAGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcatc      <  1:2824519/150‑1 (MQ=255)
                                                                                                                                                 accaTACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcatcatca  >  1:4361302/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCA  >  CP009273/3910747‑3911041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑