Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,247 A→T 71.0% V57E (GTG→GAG)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,2470AT71.0% 554.7 / 306.3 326V57E (GTG→GAG) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (36/58);  new base T (92/139);  total (128/198)
Fisher's exact test for biased strand distribution p-value = 9.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

TTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTA  >  CP009273/3994098‑3994393
                                                                                                                                                     |                                                                                                                                                  
  tCGATGTACACACCGAGCGGCGCAGAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTcc                                                                                                                                                  <  2:4582396/150‑1 (MQ=255)
  tCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTCAGATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCAcc                                                                                                                                                  <  2:4510521/150‑1 (MQ=255)
  tCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTcc                                                                                                                                                  >  2:22293/1‑150 (MQ=255)
   cGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTATTGCCAGTACATTTTCTccc                                                                                                                                                 <  2:851091/150‑1 (MQ=255)
    gATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCCGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCg                                                                                                                                                >  2:2937117/1‑150 (MQ=255)
         aCAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTg                                                                                                                                           <  2:1396224/150‑1 (MQ=255)
           aaaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGtt                                                                                                                                         >  1:1744998/1‑150 (MQ=255)
           aaaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGtt                                                                                                                                         <  2:4737426/150‑1 (MQ=255)
           aaaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGtt                                                                                                                                         <  1:2218684/150‑1 (MQ=255)
            aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                        >  2:2075300/1‑150 (MQ=255)
            aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                        >  2:4678499/1‑150 (MQ=255)
             aCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTg                                                                                                                                       <  2:1528731/150‑1 (MQ=255)
              ccGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGa                                                                                                                                      <  1:4522369/150‑1 (MQ=255)
               cGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAg                                                                                                                                     <  1:2926467/150‑1 (MQ=255)
                  gcggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAt                                                                                                                                  <  2:3600572/150‑1 (MQ=255)
                  gcggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGGTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAt                                                                                                                                  <  2:652252/150‑1 (MQ=255)
                   cggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAtt                                                                                                                                 <  2:2555552/150‑1 (MQ=255)
                   cggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTGTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAtt                                                                                                                                 >  2:2663198/1‑150 (MQ=255)
                    ggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTa                                                                                                                                >  2:1890790/1‑150 (MQ=255)
                    ggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTa                                                                                                                                <  1:1526493/150‑1 (MQ=255)
                     gcgcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTaa                                                                                                                               >  2:2871289/1‑150 (MQ=255)
                      cgcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAAc                                                                                                                              >  1:5178194/1‑150 (MQ=255)
                         aTAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGt                                                                                                                           >  2:3748815/1‑150 (MQ=255)
                         aTAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGt                                                                                                                           <  1:3567835/150‑1 (MQ=255)
                           aGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                         <  2:3921646/150‑1 (MQ=255)
                            gCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtata                                                                                                                        <  1:3390087/150‑1 (MQ=255)
                            gCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtata                                                                                                                        <  2:1818186/150‑1 (MQ=255)
                              tttCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACg                                                                                                                      >  2:2007978/1‑150 (MQ=255)
                              tttCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACg                                                                                                                      <  1:3623069/150‑1 (MQ=255)
                                  atcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTg                                                                                                                  <  2:5174874/150‑1 (MQ=255)
                                  atcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTg                                                                                                                  <  1:264624/150‑1 (MQ=255)
                                   tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGt                                                                                                                 >  1:3256270/1‑150 (MQ=255)
                                   tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGt                                                                                                                 >  1:486533/1‑150 (MQ=255)
                                   tcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGt                                                                                                                 >  1:1843427/1‑150 (MQ=255)
                                     atAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTAc                                                                                                               <  1:4069451/150‑1 (MQ=255)
                                       aGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACgg                                                                                                             <  1:5095202/150‑1 (MQ=255)
                                               ccgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTc                                                                                                     <  1:91900/150‑1 (MQ=255)
                                                 gTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCgg                                                                                                   >  1:1154473/1‑150 (MQ=255)
                                                    cATCGCCATCTGCAACTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACTCTTCGCCATGGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATc                                                                                                <  2:5001971/150‑1 (MQ=255)
                                                     aTCGCCATCTGCACCTGACCCTGACTATTCATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCt                                                                                               <  1:2727929/150‑1 (MQ=255)
                                                     aTCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCt                                                                                               >  1:4959336/1‑150 (MQ=255)
                                                     aTCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCt                                                                                               <  1:210347/150‑1 (MQ=255)
                                                      tCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCTAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTg                                                                                              <  2:4306669/150‑1 (MQ=255)
                                                          cATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGagag                                                                                          >  1:3926248/1‑150 (MQ=255)
                                                           aTCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTCTACGCCTGTACGGTCAGCGTCGGATCTGAGAGt                                                                                         >  2:1286325/1‑150 (MQ=255)
                                                            tCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTg                                                                                        >  1:4121557/1‑150 (MQ=255)
                                                                   ctgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatc                                                                                 <  1:2198991/150‑1 (MQ=255)
                                                                   ctgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatc                                                                                 <  1:3014169/150‑1 (MQ=255)
                                                                      accctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatcatc                                                                              >  1:1797888/1‑150 (MQ=255)
                                                                         ctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGc                                                                           <  1:2832298/150‑1 (MQ=255)
                                                                          tgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCg                                                                          >  2:777330/1‑150 (MQ=255)
                                                                           gacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCgg                                                                         >  1:1912031/1‑150 (MQ=255)
                                                                            acTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGc                                                                        >  2:5073476/1‑150 (MQ=255)
                                                                              tATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCaa                                                                      <  2:5114846/150‑1 (MQ=255)
                                                                               aTTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCCTCAGCGGCAAc                                                                     <  2:2379582/150‑1 (MQ=255)
                                                                               aTTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAAc                                                                     >  1:1961085/1‑150 (MQ=255)
                                                                                  aaTATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTGTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGAACTGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGc                                                                  >  1:332112/1‑150 (MQ=255)
                                                                                    tatCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCaa                                                                <  2:2301686/150‑1 (MQ=255)
                                                                                     atCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCaaa                                                               <  2:4896914/150‑1 (MQ=255)
                                                                                        cGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACaa                                                            >  1:892629/1‑150 (MQ=255)
                                                                                         gCCAGCAGAGCAAGTCACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCATTACAATTTCTCCCTCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAg                                                           <  2:2812338/150‑1 (MQ=255)
                                                                                            agcagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGa                                                        >  1:346465/1‑150 (MQ=255)
                                                                                              cagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAg                                                      <  2:439271/150‑1 (MQ=255)
                                                                                              cagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAg                                                      >  1:3158564/1‑150 (MQ=255)
                                                                                               agagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                     <  2:2176095/150‑1 (MQ=255)
                                                                                               agagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                     >  1:1477131/1‑150 (MQ=255)
                                                                                               agagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                     >  1:670560/1‑150 (MQ=255)
                                                                                               agagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                     <  2:806904/150‑1 (MQ=255)
                                                                                                 agCATGTAACGGTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTaa                                                   <  2:3528731/150‑1 (MQ=255)
                                                                                                    aTGTAACGGTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGt                                                <  2:3259807/150‑1 (MQ=255)
                                                                                                       tAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAg                                             <  2:1125058/150‑1 (MQ=255)
                                                                                                         aCGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAggg                                           <  2:2419854/150‑1 (MQ=255)
                                                                                                           gTTCCCCACGCTTCGCCCGTGGCTTGGTGCCAGGACATGGTCACCCGCTCTGGGTGAGGATTACCGGTCTACGCCGGGACGGTCAGCGTCGGATCGGAGAGTGCGCAATCATCAGCGGCACCAGCCACCCAGGGCAGTCCGGGGAGGGCg                                         >  2:3162418/1‑150 (MQ=255)
                                                                                                            ttCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCga                                        <  1:4586052/150‑1 (MQ=255)
                                                                                                            ttCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCga                                        <  1:4155088/150‑1 (MQ=255)
                                                                                                              ccccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCgaga                                      <  2:3100722/150‑1 (MQ=255)
                                                                                                                 cACGCTTCGCCATGGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaaa                                   <  2:4314273/150‑1 (MQ=255)
                                                                                                                   cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                 <  2:1582927/150‑1 (MQ=255)
                                                                                                                   cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                 <  1:5070243/150‑1 (MQ=255)
                                                                                                                   cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                 <  1:2112875/150‑1 (MQ=255)
                                                                                                                    gCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATc                                <  1:2077937/150‑1 (MQ=255)
                                                                                                                     cTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATcc                               <  1:2159616/150‑1 (MQ=255)
                                                                                                                     cTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATcc                               >  1:3830307/1‑150 (MQ=255)
                                                                                                                       tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGt                             >  1:1696656/1‑150 (MQ=255)
                                                                                                                       tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGt                             >  1:4803604/1‑150 (MQ=255)
                                                                                                                         gCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                           >  2:481786/1‑150 (MQ=255)
                                                                                                                          ccATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGCAGTAAGGGGAGGGCGAGACATATCCGTTTg                          >  2:3662097/1‑150 (MQ=255)
                                                                                                                          ccATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTg                          >  1:45998/1‑150 (MQ=255)
                                                                                                                          ccATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTg                          <  1:2249256/150‑1 (MQ=255)
                                                                                                                           cATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTga                         <  2:2490733/150‑1 (MQ=255)
                                                                                                                                 cTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCa                   >  1:4135184/1‑150 (MQ=255)
                                                                                                                                  tttttGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACTGTCAGCGTCGGATCTGAGAGTGCTCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCaa                  <  2:4620077/150‑1 (MQ=255)
                                                                                                                                           gTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAt         <  2:4462232/150‑1 (MQ=255)
                                                                                                                                           gTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAt         <  2:1151432/150‑1 (MQ=255)
                                                                                                                                            tACATTTTCTCCCGCTCTGTTTGAGGTTTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAtt        <  1:1051607/150‑1 (MQ=255)
                                                                                                                                            tACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAtt        >  1:2312080/1‑150 (MQ=255)
                                                                                                                                              cATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTcc      <  1:4276218/150‑1 (MQ=255)
                                                                                                                                                ttttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCtt    <  1:53655/150‑1 (MQ=255)
                                                                                                                                                 tttCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTATGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCttt   <  2:4178185/150‑1 (MQ=255)
                                                                                                                                                  ttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTa  <  2:4949801/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTA  >  CP009273/3994098‑3994393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑