BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,127,022	T→G	63.4%	E188D (GAA→GAC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,127,022	0	T	G	63.4%	357.6 / 290.1	267	E188D (GAA→GAC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand): ref base T (61/37); new base G (84/85); total (145/122)
Fisher's exact test for biased strand distribution p-value = 5.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

TGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCT  >  CP009273/4126874‑4127171

tgattgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACAtctt                                                                                                                                                      <  1:3930634/150‑1 (MQ=255)

          ggCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTgcgc                                                                                                                                            <  2:4883045/150‑1 (MQ=255)

             aCTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCCGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGGTAAACAGCACATCGTCTTCCTGCGCGcc                                                                                                                                         >  2:3058249/1‑150 (MQ=255)

              cTGGCGCTTGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCt                                                                                                                                        <  2:3390737/150‑1 (MQ=255)

               tGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTg                                                                                                                                       <  1:1023800/150‑1 (MQ=255)

               tGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTg                                                                                                                                       <  1:2490655/150‑1 (MQ=255)

                ggCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTgc                                                                                                                                      <  1:476733/150‑1 (MQ=255)

                  cgcTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTgcgc                                                                                                                                    >  1:4923474/1‑150 (MQ=255)

                       gTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATg                                                                                                                               <  2:528855/150‑1 (MQ=255)

                        tGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGt                                                                                                                              >  2:1784789/1‑150 (MQ=255)

                            ccGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATg                                                                                                                          <  1:2421977/150‑1 (MQ=255)

                               tAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTAt                                                                                                                       >  1:2460455/1‑150 (MQ=255)

                               tAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTAt                                                                                                                       >  1:562587/1‑150 (MQ=255)

                                 aGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCa                                                                                                                     <  1:4413990/150‑1 (MQ=255)

                                 aGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCa                                                                                                                     <  1:3363456/150‑1 (MQ=255)

                                  gAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCAc                                                                                                                    <  1:4827040/150‑1 (MQ=255)

                                   aaaaaaGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCAcc                                                                                                                   >  1:2005354/1‑150 (MQ=255)

                                     aaaaGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATAGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAg                                                                                                                 >  2:1556558/1‑150 (MQ=255)

                                       aagaagCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAgcg                                                                                                               >  1:1780664/1‑150 (MQ=255)

                                       aagaagCCGTCAGTGTAAAAAGGATCTGAATTTTTTCGTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAgcg                                                                                                               <  2:4481410/150‑1 (MQ=255)

                                          aagCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAgcggcg                                                                                                            <  2:138429/150‑1 (MQ=255)

                                           agCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGt                                                                                                           >  2:1155323/1‑150 (MQ=255)

                                           agCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGt                                                                                                           <  2:202135/150‑1 (MQ=255)

                                            gCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTa                                                                                                          <  2:4191183/150‑1 (MQ=255)

                                             ccGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTAc                                                                                                         <  2:3499391/150‑1 (MQ=255)

                                             ccGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTAc                                                                                                         >  1:1399348/1‑150 (MQ=255)

                                              cGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTAcc                                                                                                        >  2:4453967/1‑150 (MQ=255)

                                              cGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTAcc                                                                                                        <  1:2933180/150‑1 (MQ=255)

                                              cGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTAcc                                                                                                        >  2:2112415/1‑150 (MQ=255)

                                               gTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCg                                                                                                       >  2:3985966/1‑150 (MQ=255)

                                               gTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCg                                                                                                       >  1:2241658/1‑150 (MQ=255)

                                               gTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCg                                                                                                       <  1:1523442/150‑1 (MQ=255)

                                                 cAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTc                                                                                                     >  1:1543764/1‑150 (MQ=255)

                                                     gtAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCgg                                                                                                 >  2:5024078/1‑150 (MQ=255)

                                                      tAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCggg                                                                                                <  1:997180/150‑1 (MQ=255)

                                                       aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                               >  2:5046510/1‑150 (MQ=255)

                                                         aaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGa                                                                                             <  2:3093927/150‑1 (MQ=255)

                                                            ggATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGc                                                                                          <  1:4364391/150‑1 (MQ=255)

                                                              aTCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCa                                                                                        >  1:3058775/1‑150 (MQ=255)

                                                               tCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCAt                                                                                       >  1:1655091/1‑150 (MQ=255)

                                                                 tGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCt                                                                                     >  2:4536483/1‑150 (MQ=255)

                                                                  gAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTg                                                                                    >  2:1686211/1‑150 (MQ=255)

                                                                   aaTTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGa                                                                                   <  1:4653354/150‑1 (MQ=255)

                                                                   aaTTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGa                                                                                   <  1:2049884/150‑1 (MQ=255)

                                                                     ttttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGACGCGGGCGAAGCCATCTGaca                                                                                 >  2:3940091/1‑148 (MQ=255)

                                                                     ttttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGAGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCACTCTGaaa                                                                                 >  2:366731/1‑150 (MQ=255)

                                                                      tttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAac                                                                                >  2:2140256/1‑150 (MQ=255)

                                                                       ttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAacc                                                                               >  2:2738580/1‑150 (MQ=255)

                                                                        tttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAAGGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAacca                                                                              <  2:3492199/150‑1 (MQ=255)

                                                                         ttcttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccac                                                                             >  1:3445835/1‑150 (MQ=255)

                                                                         ttcttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAaccac                                                                             >  1:4046109/1‑150 (MQ=255)

                                                                            ttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTCTGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAcccc                                                                          >  2:2873203/1‑150 (MQ=255)

                                                                             tcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCa                                                                         <  1:4182978/150‑1 (MQ=255)

                                                                             tcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCa                                                                         <  1:2320395/150‑1 (MQ=255)

                                                                                     ggCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGc                                                                 <  1:5138934/150‑1 (MQ=255)

                                                                                      gCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGcc                                                                >  1:5173812/1‑150 (MQ=255)

                                                                                        gATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAg                                                              >  1:4464820/1‑150 (MQ=255)

                                                                                         aTCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGc                                                             >  2:4035765/1‑150 (MQ=255)

                                                                                          tctcTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGcc                                                            <  1:2211969/150‑1 (MQ=255)

                                                                                             ctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGt                                                         >  1:4283360/1‑150 (MQ=255)

                                                                                               tATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGGTa                                                       >  1:3516737/1‑150 (MQ=255)

                                                                                                aTTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTAt                                                      <  1:3619681/150‑1 (MQ=255)

                                                                                                aTTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTAt                                                      <  1:877233/150‑1 (MQ=255)

                                                                                                  ttAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATcc                                                    <  1:823214/150‑1 (MQ=255)

                                                                                                    aaCAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAg                                                  >  1:4820693/1‑150 (MQ=255)

                                                                                                    aaCAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAg                                                  <  2:1543764/150‑1 (MQ=255)

                                                                                                     aCAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGt                                                 >  1:686618/1‑150 (MQ=255)

                                                                                                       aaaaTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCg                                               >  1:3256745/1‑150 (MQ=255)

                                                                                                          aTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATCTGTGCCAGCCCGTTATCCAGTCGCCa                                            <  2:1955178/150‑1 (MQ=255)

                                                                                                          aTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCa                                            >  2:1922671/1‑150 (MQ=255)

                                                                                                          aTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCa                                            <  2:2877348/150‑1 (MQ=255)

                                                                                                           tAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAg                                           >  1:4564944/1‑150 (MQ=255)

                                                                                                            aaCGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAgg                                          <  1:2976295/150‑1 (MQ=255)

                                                                                                               gATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGgaga                                       >  1:1965071/1‑150 (MQ=255)

                                                                                                               gATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGgaga                                       >  1:5161459/1‑150 (MQ=255)

                                                                                                                aTAATGCCCCACCCTCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGAc                                      <  2:803188/150‑1 (MQ=255)

                                                                                                                   aTGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAat                                   <  2:5174547/150‑1 (MQ=255)

                                                                                                                      ccccACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATc                                <  2:4241334/150‑1 (MQ=255)

                                                                                                                      ccccACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATc                                <  2:4769164/150‑1 (MQ=255)

                                                                                                                        ccaccaTCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGc                              >  1:3866384/1‑150 (MQ=255)

                                                                                                                            caTCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGc                          >  1:2586521/1‑150 (MQ=255)

                                                                                                                            caTCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGc                          >  1:3257477/1‑150 (MQ=255)

                                                                                                                             aTCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCt                         >  1:1211315/1‑150 (MQ=255)

                                                                                                                             aTCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCt                         >  1:666504/1‑150 (MQ=255)

                                                                                                                                cGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACACTATCGCCCGCTTGa                      >  2:1368980/1‑150 (MQ=255)

                                                                                                                                cGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCATTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGa                      >  2:1086615/1‑150 (MQ=255)

                                                                                                                                cGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCATTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGa                      >  2:1333731/1‑150 (MQ=255)

                                                                                                                                   cAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACCATATCGCCCGCTTGATAg                   >  2:5154816/1‑150 (MQ=255)

                                                                                                                                     gTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACACTATCGCCAGCTTGATTGTc                 >  2:2826324/1‑150 (MQ=255)

                                                                                                                                     gTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc                 >  2:2541661/1‑150 (MQ=255)

                                                                                                                                     gTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc                 <  1:2153631/150‑1 (MQ=255)

                                                                                                                                     gTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc                 >  2:3296919/1‑150 (MQ=255)

                                                                                                                                     gTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc                 <  2:2241658/150‑1 (MQ=255)

                                                                                                                                      ttAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAAGGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCa                >  2:2371086/1‑150 (MQ=255)

                                                                                                                                       tAAACTGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCAc               <  2:3736320/150‑1 (MQ=255)

                                                                                                                                        aaaCAGCACATCTTCTTCCTTCGCGCCTGCGCGAATGTTATTTATCACCAGCGGCGTACAGTCGCTGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCATTCGCCAGGAGAAAATATCTCCCGCTTGATAGTCaat              >  2:4927895/1‑148 (MQ=255)

                                                                                                                                          aCAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGGACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTgg            <  1:3788209/150‑1 (MQ=255)

                                                                                                                                              cacaTCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt        <  1:5045831/150‑1 (MQ=255)

                                                                                                                                              cacaTCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt        <  1:2349467/150‑1 (MQ=255)

                                                                                                                                               acaTCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTc       >  1:1975224/1‑150 (MQ=255)

TGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCT  >  CP009273/4126874‑4127171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`