Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 351,359 G→T 18.6% G243C (GGT→TGT)  codB → cytosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273351,3590GT18.6% 842.5 / 145.7 306G243C (GGT→TGT) codBcytosine transporter
Reads supporting (aligned to +/- strand):  ref base G (102/147);  new base T (28/29);  total (130/176)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 309 total aligned reads displayed.

ATGTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCC  >  CP009273/351213‑351508
                                                                                                                                                  |                                                                                                                                                     
aTGTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTg                                                                                                                                                    <  2:720257/150‑1 (MQ=255)
  gTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCTGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCa                                                                                                                                                  >  2:5080115/1‑150 (MQ=255)
  gTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCa                                                                                                                                                  <  2:959458/150‑1 (MQ=255)
  gTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCa                                                                                                                                                  >  2:1593598/1‑150 (MQ=255)
    cgcgCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGc                                                                                                                                                <  2:635377/150‑1 (MQ=255)
      cgctggcgctggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCgg                                                                                                                                              <  2:3988797/150‑1 (MQ=255)
       gctggcgctggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCggg                                                                                                                                             <  1:1723320/150‑1 (MQ=255)
          ggcgctggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTTACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGgcgc                                                                                                                                          >  1:2704132/1‑150 (MQ=255)
           gcgctggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCt                                                                                                                                         <  1:2426095/150‑1 (MQ=255)
            cgctggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTg                                                                                                                                        >  1:1781151/1‑150 (MQ=255)
             gctggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGc                                                                                                                                       >  1:1449892/1‑150 (MQ=255)
                ggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGc                                                                                                                                    <  2:1573227/150‑1 (MQ=255)
                ggTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGc                                                                                                                                    <  1:4563371/150‑1 (MQ=255)
                 ggtgtgGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCa                                                                                                                                   >  2:5217524/3‑150 (MQ=255)
                 gTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCa                                                                                                                                   >  2:452333/1‑150 (MQ=255)
                   tgtgGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACt                                                                                                                                 <  2:1108904/150‑1 (MQ=255)
                   tgtgGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACt                                                                                                                                 <  1:4314232/150‑1 (MQ=255)
                   tgtgGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACt                                                                                                                                 >  1:2907045/1‑150 (MQ=255)
                        ggTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCa                                                                                                                            <  2:2566628/150‑1 (MQ=255)
                            aTTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGc                                                                                                                        >  1:3110254/1‑150 (MQ=255)
                             tttATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCg                                                                                                                       >  2:1304255/1‑150 (MQ=255)
                             tttATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCg                                                                                                                       >  1:4511146/1‑150 (MQ=255)
                             tttATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCg                                                                                                                       <  2:1250098/150‑1 (MQ=255)
                               tATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCATTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGa                                                                                                                     <  1:771798/150‑1 (MQ=255)
                                aTCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGAAGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGat                                                                                                                    >  1:3209020/1‑150 (MQ=255)
                                 tCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGata                                                                                                                   <  1:929308/150‑1 (MQ=255)
                                      gCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATctct                                                                                                              <  1:3499071/150‑1 (MQ=255)
                                       cGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAAGTCGTTGTGGTTGATGTTCGGTGCAGCGGGCGCTGCGGCACTGGGCCTGGCGGAGCtctcgg                                                                                                             >  2:2714926/1‑148 (MQ=255)
                                       cGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCtg                                                                                                             >  1:2816291/1‑150 (MQ=255)
                                         ggTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCtgat                                                                                                           <  1:4677640/150‑1 (MQ=255)
                                           tACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCtgatgt                                                                                                         <  1:954684/150‑1 (MQ=255)
                                            aCGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCTGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCtgatgtg                                                                                                        >  2:4598264/1‑150 (MQ=255)
                                                     gCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCt                                                                                               >  1:2632427/1‑150 (MQ=255)
                                                     gCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCt                                                                                               >  1:1757681/1‑150 (MQ=255)
                                                       tGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCa                                                                                             >  1:3418833/1‑150 (MQ=255)
                                                          cTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAggg                                                                                          <  2:123069/150‑1 (MQ=255)
                                                            ttGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGcc                                                                                        >  1:4943694/1‑150 (MQ=255)
                                                            ttGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGcc                                                                                        <  1:364683/150‑1 (MQ=255)
                                                              gTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCctg                                                                                      <  2:2217214/150‑1 (MQ=255)
                                                                ccGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCctgct                                                                                    <  1:2964624/150‑1 (MQ=255)
                                                                 cGGTTTGGTCGCAATGCCAAACTGGCGGTTCTGTTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGTCGCTGCGGCACTGGGCATTGCGGATATCTCTGATGTGATGATTGCTCAGGGCctgctg                                                                                   <  1:81692/150‑1 (MQ=255)
                                                                 cGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCctgctg                                                                                   >  1:1288805/1‑150 (MQ=255)
                                                                  ggTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCctgctgc                                                                                  >  1:243862/1‑150 (MQ=255)
                                                                   gTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCctgctgct                                                                                 <  2:66891/150‑1 (MQ=255)
                                                                     ttGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCtgctgctgc                                                                               <  1:1523333/150‑1 (MQ=255)
                                                                       ggTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGctgcct                                                                             <  2:1506111/150‑1 (MQ=255)
                                                                       ggTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGctgcct                                                                             <  2:2436136/150‑1 (MQ=255)
                                                                        ctCGCACTGCCACACTGCCGGGCCAGGGGGCGATGGTCCCCGTCTTCCTCGGCAACTCCATGAGGAGTAGCTTCGGAGCAGCGGGCGCAGCGGCACTGGGCATGGCGGATATCTCTGACGTGATGATTGCTCAGGGCCTGCTGctgcctg                                                                            <  2:3144229/149‑1 (MQ=255)
                                                                         tCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGctgcctgc                                                                           <  2:4656429/150‑1 (MQ=255)
                                                                         tCGCAAGGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTGTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGctgcctgc                                                                           <  2:1667984/150‑1 (MQ=255)
                                                                            cAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGAt                                                                        <  2:3769921/150‑1 (MQ=255)
                                                                             aaTGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGAtt                                                                       <  1:1025389/150‑1 (MQ=255)
                                                                                 ccAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTgtgg                                                                   >  1:3455638/1‑150 (MQ=255)
                                                                                  cAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTgtggt                                                                  <  1:4144795/150‑1 (MQ=255)
                                                                                  cAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTgtggt                                                                  >  2:4957171/1‑150 (MQ=255)
                                                                                    aaCTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGc                                                                >  1:4876506/1‑150 (MQ=255)
                                                                                     aCTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCt                                                               <  1:3307264/150‑1 (MQ=255)
                                                                                     aCTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCt                                                               >  1:1999874/1‑150 (MQ=255)
                                                                                       tGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTgg                                                             >  2:3007389/1‑150 (MQ=255)
                                                                                         gCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTgggg                                                           <  2:3558615/150‑1 (MQ=255)
                                                                                           ggTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCt                                                         >  1:3262460/1‑150 (MQ=255)
                                                                                            gTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTg                                                        <  1:4085206/150‑1 (MQ=255)
                                                                                             tGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGa                                                       <  2:2346707/150‑1 (MQ=255)
                                                                                               cTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAat                                                     <  2:146238/150‑1 (MQ=255)
                                                                                                tggtggCTATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAata                                                    <  1:4793888/150‑1 (MQ=255)
                                                                                                tggtggCTATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAata                                                    <  1:2192222/150‑1 (MQ=255)
                                                                                                tggtggCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAata                                                    <  1:4064637/150‑1 (MQ=255)
                                                                                                tggtggCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAata                                                    <  2:4666966/150‑1 (MQ=255)
                                                                                                  gtggCGATGGGGGCCTTTTTCCTCGGCAACTCGTGGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATc                                                  <  2:2671053/150‑1 (MQ=255)
                                                                                                   tggCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAATATCt                                                 <  2:2858387/150‑1 (MQ=255)
                                                                                                     gCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCCGAATATCTgg                                               <  1:4241240/150‑1 (MQ=255)
                                                                                                     gCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAATATCTgg                                               <  1:3567769/150‑1 (MQ=255)
                                                                                                        aTGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGacc                                            >  1:4118045/1‑150 (MQ=255)
                                                                                                          ggtggCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGaccac                                          <  2:3365666/150‑1 (MQ=255)
                                                                                                           gtggCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGaccacc                                         <  1:2506045/150‑1 (MQ=255)
                                                                                                            tggCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGAccacca                                        >  2:4410084/1‑150 (MQ=255)
                                                                                                              gCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAAc                                      <  2:2994030/150‑1 (MQ=255)
                                                                                                               ccTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACg                                     <  1:628107/150‑1 (MQ=255)
                                                                                                                cTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGa                                    <  2:1700273/150‑1 (MQ=255)
                                                                                                                cTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAATATCTGGACCACCAACGa                                    >  1:2173087/1‑150 (MQ=255)
                                                                                                                  ttttCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATa                                  >  1:1066901/1‑150 (MQ=255)
                                                                                                                  ttttCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAATATCTGGACCACCAACGATa                                  <  1:3794500/150‑1 (MQ=255)
                                                                                                                    ttCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCAGGGGCTGAATATCTGGACCACCAACGATAAc                                <  1:2302188/150‑1 (MQ=255)
                                                                                                                     tCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACg                               >  2:4470102/1‑150 (MQ=255)
                                                                                                                     tCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACg                               >  1:5172363/1‑150 (MQ=255)
                                                                                                                          ggCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCActc                          >  1:2137806/1‑150 (MQ=255)
                                                                                                                            cAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTa                        >  2:734133/1‑150 (MQ=255)
                                                                                                                            cAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTa                        >  1:3672497/1‑150 (MQ=255)
                                                                                                                            cAACTCGATGATGTTTAATTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTTAACAGGGCCAGCTGCTGCCTGCGAATGTGGTGCTGGGGCTGAATATCTGGACCACCAACCATAACGCACTCTa                        >  2:4371938/1‑150 (MQ=255)
                                                                                                                             aaCTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTAt                       >  2:4791041/1‑150 (MQ=255)
                                                                                                                                 cGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCTCCAACGATAACGCACTCTCTGCGt                   >  2:4033780/1‑150 (MQ=255)
                                                                                                                                  ggggATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATCTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATACCGCACTCTATGCGTc                  >  2:2977281/4‑150 (MQ=255)
                                                                                                                                  gTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTc                  <  1:5149082/150‑1 (MQ=255)
                                                                                                                                    tGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCgg                >  1:1944559/1‑150 (MQ=255)
                                                                                                                                     gATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCggg               >  2:1415997/1‑150 (MQ=255)
                                                                                                                                     gATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCggg               >  1:2986759/1‑150 (MQ=255)
                                                                                                                                         tttATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTa           <  1:2567090/150‑1 (MQ=255)
                                                                                                                                           tATTTTCTGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAgg         >  1:4408421/1‑150 (MQ=255)
                                                                                                                                            aTTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGt        >  2:1914749/1‑150 (MQ=255)
                                                                                                                                              tttCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGttt      <  1:5034216/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATGTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTTATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCC  >  CP009273/351213‑351508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑