BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,285,857	T→C	5.6%	intergenic (+135/+396)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,285,857	0	T	C	5.6%	428.1 / 14.2	146	intergenic (+135/+396)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base T (62/73); new base C (6/2); total (70/76)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 149 total aligned reads displayed.

ACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTG  >  CP009273/4285708‑4286005

aCTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                                                                                                                                                      <  1:2817327/150‑1 (MQ=255)

aCTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                                                                                                                                                      <  2:2314289/150‑1 (MQ=255)

  tGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                                                                                                                                    <  2:3920955/150‑1 (MQ=255)

  tGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                                                                                                                                    <  2:2230518/150‑1 (MQ=255)

   tcGGATCAATTATTGCAGATTGCCGTGGTTATCCACCCTGCTATGTTTGAATGCCTGATTCGACGCTTGCCTCGTCTTTTCAGTCATACGCCAGTCAGGGCTATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                                                                                                                   <  2:2413124/149‑1 (MQ=17)

    cGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                                                                  >  1:833200/1‑150 (MQ=255)

    cGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                                                                  >  1:1985974/1‑150 (MQ=255)

     ggATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTAATCAGGCCTCCGCCAGACAGCGCCATAGCCTGATTTAGCTTGATTTAGTAGGTCGGATAAGGCGATTATGACGc                                                                                                                                                 >  2:2813601/1‑150 (MQ=21)

       aTCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAt                                                                                                                                               >  1:386158/1‑150 (MQ=255)

          aataatTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                                                                                                                            >  1:1919257/1‑150 (MQ=255)

           ataatTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa                                                                                                                                           >  1:2267224/1‑150 (MQ=255)

           ataatTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa                                                                                                                                           >  1:1309529/1‑150 (MQ=255)

            taatTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAc                                                                                                                                          >  1:4508652/1‑150 (MQ=255)

              atTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACAt                                                                                                                                        >  1:5170460/1‑150 (MQ=255)

              atTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAAAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACAt                                                                                                                                        >  1:5165482/1‑150 (MQ=255)

               ttGCAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                                                                                                                       <  2:3764572/150‑1 (MQ=255)

               ttGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATc                                                                                                                                       >  1:2711213/1‑150 (MQ=255)

               ttGAAGATTGCAGGTGATATACACCCGGCAATGTGTTAATTCCTGATGAGACGCTTGACGCGTCTAATAAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCTTTTATGCGTCTTCAGACATc                                                                                                                                       >  2:3741933/1‑150 (MQ=17)

                  aaGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                                                                                                                    <  2:372323/150‑1 (MQ=255)

                  aaGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                                                                                                                    <  2:1124219/150‑1 (MQ=255)

                  aaGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                                                                                                                    <  2:1124072/150‑1 (MQ=255)

                  aaGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                                                                                                                    >  1:1731730/1‑150 (MQ=255)

                  aaGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAAc                                                                                                                                    >  2:2410671/1‑150 (MQ=255)

                   aGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACg                                                                                                                                   >  2:599590/1‑150 (MQ=255)

                     aTTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGcc                                                                                                                                 <  1:837522/150‑1 (MQ=255)

                      ttGCCGGGGATCTCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt                                                                                                                                <  2:3538216/150‑1 (MQ=255)

                      tggCCGGGGATATCAACCCGGCATTGTGTGAATGCCTGATGCGTCGCTTGCCGCGTATTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt                                                                                                                                <  2:3221349/148‑1 (MQ=255)

                       tGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                                                                                                                               <  2:2848161/150‑1 (MQ=255)

                       tGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                                                                                                                               <  1:2252819/150‑1 (MQ=255)

                       tGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGAATCCGACATCAACGCCTg                                                                                                                               >  2:3482894/1‑150 (MQ=255)

                         ccGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                                                                                                                             <  2:3364283/150‑1 (MQ=255)

                         ccGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                                                                                                                             <  2:284264/150‑1 (MQ=255)

                         ccGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                                                                                                                             <  2:4269787/150‑1 (MQ=255)

                         ccGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATACGACTTAAACGCCGGAt                                                                                                                             >  1:1562742/1‑150 (MQ=255)

                          cGGGGATATCCACCCGGCAATGTGTGAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCTACATCAACGCCTGATg                                                                                                                            <  2:2100198/150‑1 (MQ=255)

                             ggATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                         >  2:206499/1‑150 (MQ=255)

                             ggATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                         <  1:4304276/150‑1 (MQ=255)

                             ggATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                         <  1:1933297/150‑1 (MQ=255)

                                 atCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                     >  1:2725937/1‑150 (MQ=255)

                                 atCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                     >  1:3177979/1‑150 (MQ=255)

                                 atCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                     <  2:1060007/150‑1 (MQ=255)

                                 atCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                     <  2:758671/150‑1 (MQ=255)

                                  tCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCtt                                                                                                                    <  1:2292449/150‑1 (MQ=255)

                                   ccACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAAGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTa                                                                                                                   <  2:1169857/150‑1 (MQ=255)

                                    cACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACTCCTGATGCGACGCTTaa                                                                                                                  >  1:3929430/1‑150 (MQ=255)

                                    cACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                  >  2:133780/1‑150 (MQ=255)

                                      cccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcg                                                                                                                >  2:334193/1‑150 (MQ=255)

                                       ccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                                                                                                               <  2:723033/150‑1 (MQ=255)

                                       ccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                                                                                                               <  2:4559468/150‑1 (MQ=255)

                                       ccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                               <  1:1195156/134‑1 (MQ=255)

                                       ccGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                               >  2:1195156/1‑134 (MQ=255)

                                         ggCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTTActagt                                                                                                             >  2:3793883/1‑146 (MQ=255)

                                         ggCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                                                                                                             <  2:4021997/150‑1 (MQ=40)

                                          gCACTGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc                                                                                                            <  2:2234457/150‑1 (MQ=38)

                                           cAATGTGTGTATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt                                                                                                           <  1:1954171/150‑1 (MQ=37)

                                           cAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt                                                                                                           >  1:1321164/1‑150 (MQ=39)

                                           cAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt                                                                                                           <  2:2711213/150‑1 (MQ=255)

                                            aaTGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCtt                                                                                                          >  1:4368801/1‑150 (MQ=38)

                                            aaTGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATGATTAAGCGTGATTATGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCtt                                                                                                          >  1:4369712/1‑150 (MQ=255)

                                             aTGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGATTAACGCGTCtaa                                                                                                         >  1:3513260/1‑148 (MQ=255)

                                              tgtgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAt                                                                                                        <  2:3393135/150‑1 (MQ=37)

                                               gtgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACTCCTGAGGCGACGCTTAACGCGTCTTATc                                                                                                       >  2:3517399/1‑150 (MQ=25)

                                                tgtgAATGCCTGATGCGACACTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCa                                                                                                      <  2:4789871/150‑1 (MQ=25)

                                                 gtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                                                                     <  2:2742964/150‑1 (MQ=34)

                                                 gtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                                                                     <  2:782742/150‑1 (MQ=34)

                                                 gtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATAAACGCCTGATGCGAAGCTTAACGCGTCTTATCAg                                                                                                     >  1:2434823/1‑150 (MQ=21)

                                                 gtgAAGGCCTGTTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                                                                     <  2:4339316/150‑1 (MQ=17)

                                                  tgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAgg                                                                                                    <  2:3366553/150‑1 (MQ=33)

                                                  tgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAgg                                                                                                    <  2:4990803/150‑1 (MQ=33)

                                                    aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTTAGGAAGGAAAAGCCGATTAACACCAATCACACATAAGAGCCTGATTAGACGAGTAAAGCGTCTTATCAGGcc                                                                                                  >  1:3157143/1‑150 (MQ=2)

                                                    aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc                                                                                                  <  1:2801133/150‑1 (MQ=32)

                                                    aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc                                                                                                  >  1:3157144/1‑150 (MQ=32)

                                                    aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc                                                                                                  <  1:4222782/150‑1 (MQ=32)

                                                     aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCt                                                                                                 <  2:4040932/150‑1 (MQ=31)

                                                     aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCt                                                                                                 <  2:1699566/150‑1 (MQ=31)

                                                     aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCt                                                                                                 <  2:4040866/150‑1 (MQ=31)

                                                     aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCt                                                                                                 <  2:1699561/150‑1 (MQ=31)

                                                                                                                                   ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                   >  1:1204512/1‑150 (MQ=11)

                                                                                                                                   ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                   >  1:863678/1‑150 (MQ=11)

                                                                                                                                   ggTCGGATAAGGCGTTAATGCCGCATCCGACAGCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCAACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                   <  2:2900258/150‑1 (MQ=2)

                                                                                                                                    gTCGGATACGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                  <  2:133112/150‑1 (MQ=14)

                                                                                                                                    ctCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                  <  1:1171130/149‑1 (MQ=14)

                                                                                                                                      cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGcc                >  2:1778664/1‑150 (MQ=17)

                                                                                                                                      cGGATAAGGCGTTTATGCCGCAACCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                <  1:4404987/150‑1 (MQ=17)

                                                                                                                                       ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCAATACAGCGCAAGACCGAGAGTGAGCGTGAGTTAGTATGTCGTAGAAGGCGTTTAAGCCGCATCCGTCATCATCGCCt               >  2:1816059/1‑150 (MQ=11)

                                                                                                                                        taTAAGGCGTTTATTCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg              <  2:793786/149‑1 (MQ=17)

                                                                                                                                         aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa             >  2:313885/1‑150 (MQ=32)

                                                                                                                                         aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAATACAGCGCAATAGACTGATATAGCTTGATTATGTAGGTCTGATAAGTCGTTTAAGCCGCATACGACATCAACGCCTGa             >  2:2107522/1‑150 (MQ=11)

                                                                                                                                          tAAGGCTTTTATGCCGCATCCTACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt            <  1:45131/150‑1 (MQ=17)

                                                                                                                                           actgCGTTTATGCCGCATACGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg           <  2:2958446/147‑1 (MQ=17)

                                                                                                                                            tggCTTTTCTGCCGGAGCCGTCTTCAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc          <  2:1973862/149‑1 (MQ=11)

                                                                                                                                              gCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa        <  1:3444922/150‑1 (MQ=17)

                                                                                                                                                gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg      >  1:4581495/1‑150 (MQ=32)

                                                                                                                                                gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg      >  1:2542137/1‑150 (MQ=32)

                                                                                                                                                  ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt    <  2:2871440/150‑1 (MQ=32)

                                                                                                                                                  ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt    <  1:334193/150‑1 (MQ=32)

                                                                                                                                                  ttAGGCCGCATCCGTCATCACCGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt    <  2:276784/150‑1 (MQ=11)

                                                                                                                                                    aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtta  >  2:390613/1‑149 (MQ=17)

                                                                                                                                                    aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtta  >  2:390368/1‑149 (MQ=17)

                                                                                                                                                    aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg  >  1:2497229/1‑150 (MQ=32)

ACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTG  >  CP009273/4285708‑4286005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`