BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,402,613	G→T	8.0%	S137I (AGC→ATC)	yjfM →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,402,613	0	G	T	8.0%	976.5 / 3.4	330	S137I (AGC→ATC)	yjfM	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base G (114/173); new base T (36/2); total (155/175)
Fisher's exact test for biased strand distribution p-value = 2.43e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

CGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCT  >  CP009273/4402464‑4402762

cgcgcgTGGCTGGAACAACTCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAg                                                                                                                                                        <  2:3873404/150‑1 (MQ=255)

 gcgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGc                                                                                                                                                       <  2:3807968/150‑1 (MQ=255)

  cgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGcc                                                                                                                                                      >  1:1736215/1‑150 (MQ=255)

   gcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCg                                                                                                                                                     <  2:2039780/150‑1 (MQ=255)

      tGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGt                                                                                                                                                  >  1:4221514/1‑150 (MQ=255)

       ggCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGACTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGtt                                                                                                                                                 <  1:1978583/150‑1 (MQ=255)

       ggCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGtt                                                                                                                                                 <  2:1774137/150‑1 (MQ=255)

        gCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTAATCCGGGGTa                                                                                                                                                >  1:2427995/1‑150 (MQ=255)

         cTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTAt                                                                                                                                               <  2:4649103/150‑1 (MQ=255)

          tGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAAGACGGGTTAtt                                                                                                                                              >  1:1514255/1‑150 (MQ=255)

          tGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTAtt                                                                                                                                              <  2:2942151/150‑1 (MQ=255)

          tGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTAtt                                                                                                                                              <  1:2291411/150‑1 (MQ=255)

           ggAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTc                                                                                                                                             <  1:4953769/150‑1 (MQ=255)

           ggAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTATGTATTTTTTTTAATACCGGTTAtta                                                                                                                                             >  1:4846246/1‑149 (MQ=255)

            gAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTAAGCCGGGGTATTCg                                                                                                                                            >  2:26850/1‑150 (MQ=255)

                aacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGATTTTTTTTAAGCCGGGTTATTCGTaaa                                                                                                                                        >  1:247073/1‑150 (MQ=255)

                    ccAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATc                                                                                                                                    <  2:1920876/150‑1 (MQ=255)

                     cAAAACGTCATTTTATGCCGATGTTCCGAATAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATcg                                                                                                                                   <  2:1186986/150‑1 (MQ=255)

                          cGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATg                                                                                                                              <  2:1643282/150‑1 (MQ=255)

                          cGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGGTGGATTTTTTTTTATACCGGTTATTAGTAAAGATggcgtag                                                                                                                              >  1:37631/1‑147 (MQ=255)

                           ggCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTGTTAAGCCGGGGTGATCGTAAAGGTCGCGGTGa                                                                                                                             >  1:3666774/1‑150 (MQ=255)

                           ggCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTACAGATAGCGATGa                                                                                                                             >  2:1678484/1‑150 (MQ=255)

                            gCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGCTTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAg                                                                                                                            >  1:3114213/1‑150 (MQ=255)

                             cATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTTATACCGGGTATTAGTTAAGATCGCGCTGTGc                                                                                                                           >  1:8409/1‑150 (MQ=255)

                              aTTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGcc                                                                                                                          <  1:3394721/150‑1 (MQ=255)

                                  tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGTCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgttt                                                                                                                      <  1:1173903/150‑1 (MQ=255)

                                  tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgttt                                                                                                                      <  1:3295897/150‑1 (MQ=255)

                                  tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgttt                                                                                                                      <  2:90195/150‑1 (MQ=255)

                                  tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgttt                                                                                                                      <  1:4731037/150‑1 (MQ=255)

                                       cGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTta                                                                                                                >  2:800442/1‑150 (MQ=255)

                                       cGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGGTGGATCCCGGTCGTGTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGTTCGCGTTGAGCCGTTTT‑TTta                                                                                                                >  2:4934654/1‑150 (MQ=255)

                                         aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTGTTAAGCCGGGTTATTAGTAAAGATCGCGATGTGCCGTTTG‑TTttta                                                                                                              >  1:1009231/1‑150 (MQ=255)

                                         aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTtata                                                                                                              >  2:4758448/1‑150 (MQ=255)

                                          tGTTCCGAAGAATATGACTCAGCAGAACGTTCAGTCTAAGTACGAAAAATGCTACAACGACAATGTTGAACCGAGTTGGATCCCGGTCGTGTGAGGATATTAGTTATCACGGGTTATTCATAAACAAAGCCATGTGCCGATTGATTtata                                                                                                              >  2:2629510/1‑150 (MQ=255)

                                           gTTCCGAAGAATATGCCTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAAc                                                                                                            <  2:2602977/150‑1 (MQ=255)

                                             tCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAg                                                                                                          <  1:432013/150‑1 (MQ=255)

                                               cGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAgcg                                                                                                        <  1:3531921/150‑1 (MQ=255)

                                                gaagaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGACGGGTTATTCGTAAAGATAGCGATGAGCCGTTTT‑TTTATAACAgcgg                                                                                                       >  2:4577783/1‑150 (MQ=255)

                                                 aagaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAAGACGGGTTATTCGTAAATATACCGAAGATCCCTATG‑TTTATAACatccgc                                                                                                      >  1:4292524/1‑145 (MQ=255)

                                                    aaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGt                                                                                                   >  2:623928/1‑150 (MQ=255)

                                                      tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTTAGCCGGGTTATTAGTAAAGATAGCGATGTGCCCTTTG‑TTTATAAAAGcggcgggta                                                                                                 >  1:3253615/1‑147 (MQ=255)

                                                       atGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTcc                                                                                                <  1:3481882/150‑1 (MQ=255)

                                                         gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCctc                                                                                              <  1:2634526/150‑1 (MQ=255)

                                                         gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCctc                                                                                              <  1:2127292/150‑1 (MQ=255)

                                                          aCTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTTGTTTTTTTTTTAGCCGGGTTATTCGTTAATTTAGCGATTTTCCGTTTT‑TTTTTAAAAGCGGCGGTTActct                                                                                             >  1:3966294/1‑150 (MQ=255)

                                                           cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCtt                                                                                            <  2:470011/150‑1 (MQ=255)

                                                            tCAGCAGAACTGTCAGTCTAAGTACGAATATTGCTACTACGACAATTTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCttt                                                                                           <  1:4555090/150‑1 (MQ=255)

                                                                cagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCt                                                                                       <  2:4221514/150‑1 (MQ=255)

                                                                cagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGCTGGAATTTTGTTAAGCCGGGGTATTCGTAAAGGTCGCGATGAGCCGTTTG‑TTTATAACAGCGGAGGTTCCTCATTTGCt                                                                                       >  2:1118388/1‑150 (MQ=255)

                                                                 agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAAGCCGGGTTATTCGTAAAGATCGCGATTAGCCGTTTG‑TTTATAACAGCGGCGGTTCCAATTTTGcat                                                                                      >  2:3154151/1‑148 (MQ=255)

                                                                    aCTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAAGCCGGGGTATTCGTAAAGATAGCGATGAGCCGTTTG‑TTTATAAAAGCGGCGGTTGATCTTTTTCTTcgc                                                                                   >  2:235097/1‑150 (MQ=255)

                                                                       gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGGTTG‑TTTATAACAGCGGCGGTTACGCATTTGCTTAGCGcc                                                                                >  2:2485716/1‑150 (MQ=255)

                                                                        tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGccc                                                                               <  1:3036192/150‑1 (MQ=255)

                                                                          aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAg                                                                             <  1:5041118/150‑1 (MQ=255)

                                                                          aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCAGGATTTTTGTTAAGCCGGGTTATTAGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGGTCCTAATTTGCGTAGCGcccct                                                                             >  2:1424815/1‑148 (MQ=255)

                                                                          aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTAAGCCGGGGTATTCGTTAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTTCTTAGCGCCCAg                                                                             >  2:1990817/1‑150 (MQ=255)

                                                                             cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTACTCTTTTGCTTCGCGCCCAGTCt                                                                          >  2:3354209/1‑150 (MQ=255)

                                                                               aaGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGGGGATTTTTGTTAAGCCGGGGTTTTAGTAAATATCGCGATGAGCCGGTTG‑TTTATAACAACGGCGGTTGCTATTTTTCTTCGCGCCCAgacagg                                                                        >  1:2749865/1‑145 (MQ=255)

                                                                                aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTTATCCGGGTTATTCGTTAAGATAGCGATGAGCCGCTTG‑TTTATAAAAGCGGCGGTTCCTCTGTTGCTTCGCGCCCAGTCTGgc                                                                       >  2:3561116/1‑150 (MQ=255)

                                                                                        aagtGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACt                                                               <  2:2510981/147‑1 (MQ=255)

                                                                                           tGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                                            <  1:3891613/150‑1 (MQ=255)

                                                                                           tGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTTATCCCGGTTATTAGTAAAGATAGCGATGAGCCGGTTT‑TTTATAAAAGCGGCGGTTGCAATTTTTATTAGCGCCCACGCGTGCGCCGCACTGCt                                                            >  1:3990034/1‑150 (MQ=255)

                                                                                            gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATTACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCCGTCTGGCGCAGCACTTATg                                                           >  2:3299048/1‑150 (MQ=255)

                                                                                             ctactaCGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAATCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCAGGCGCAGCACTTCTgg                                                          >  2:2153776/1‑150 (MQ=255)

                                                                                             ctactaCGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg                                                          <  1:3847577/150‑1 (MQ=255)

                                                                                             ctactaCGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGTTTTTTTTTAATCCGGGTTATTAGTAAATATCGCGATGAGCCGTTTG‑TTTTTTAAAGCGGCGGTTAATCTTTTTGTTCTCGCCCAGTCTGGCGCACCACATATgg                                                          >  1:2773497/1‑150 (MQ=255)

                                                                                             ctactaCGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGATTTTTGTTAAGCCGGGTTATTAGTAAAGATCGCGATGATCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGGGCCCAGTCTGGCGCAGCACTTATgg                                                          >  2:4095303/1‑150 (MQ=255)

                                                                                                 tacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                                                      <  2:1534596/150‑1 (MQ=255)

                                                                                                   cGACAATGTTGAACAGTGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTa                                                    <  2:861190/150‑1 (MQ=255)

                                                                                                    gACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGTTTTTTTTTAAGCCGGGTTTTTAGTAAATATAGCGATGAGCCGTTTG‑TTTATAACATCGGCGGGTGCTCTTTTGCGTCGCGCCCAGTCTGGCGCAGCACATCTGGTGttttc                                                   >  1:780363/1‑148 (MQ=255)

                                                                                                        ttgttgtACAGAGTTTGATCCCGGTCGTTTCTGGATGTTTGTTTAGCCGGGTTATTCGTAATGATCGCGATGAGCCGTTTG‑TTTATTTCATCGGCGGTTCCTATTTTGCTTCGCGCCCAGTCTGGCGCAGCACTACTGGTGATTACTCCt                                               <  2:5191416/149‑1 (MQ=255)

                                                                                                        aTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCt                                               <  1:4486183/150‑1 (MQ=255)

                                                                                                        aTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCt                                               <  1:2425061/150‑1 (MQ=255)

                                                                                                           ttGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAAGCCGGGTTATTCGTAAATATAGCGATTAGCCGTTTG‑TTTATAAAAACGGCGGTTCCTCTTTTGATTAGCGCCCAGTCTGGCGCAGCCCATATGGTGATTAATCCAGgc                                            >  1:2072888/1‑150 (MQ=255)

                                                                                                             gAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTTTTAATCCGGGTTATTCGTAAAGATCGCGATGAGCCGGTTT‑TTTATAACAGCGGCGGTTACTCTTTTTCTTAGCGCCCCGTCTGGCGCAGCACATCTGGTGATTACACCTGgcgc                                          >  1:1233613/1‑150 (MQ=255)

                                                                                                                  gagTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCgg                                     <  2:4404701/150‑1 (MQ=255)

                                                                                                                    gTTGGATCCCGGTCGTTTCTTGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGtt                                   <  2:4339992/150‑1 (MQ=255)

                                                                                                                    gTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCGTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGtt                                   <  2:3431980/150‑1 (MQ=255)

                                                                                                                        gATCCCGGTCGTTTCTGGATTTTTTTTAAGCCGGGTTATTAGTAAAGATCGCGATGAGCCGTTTT‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTTGCGCAGCACTTATGGTGATTACTACTGGCGCTCCGGTTCTgg                               >  2:3702923/1‑150 (MQ=255)

                                                                                                                          tCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCa                             <  1:4591423/150‑1 (MQ=255)

                                                                                                                           cccGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaa                            <  2:728542/150‑1 (MQ=255)

                                                                                                                           cccGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaa                            <  2:2189474/150‑1 (MQ=255)

                                                                                                                           cccGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaa                            <  1:3206468/150‑1 (MQ=255)

                                                                                                                             cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAATATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTACTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTATGGCaaaa                          >  2:1818943/1‑150 (MQ=255)

                                                                                                                             cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTAGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTAGCGCCCAGTCAGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCaaaa                          >  1:1185915/1‑150 (MQ=255)

                                                                                                                               gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCacaaaa                        >  1:4835963/1‑150 (MQ=255)

                                                                                                                                 cGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAaga                      <  2:1056256/150‑1 (MQ=255)

                                                                                                                                 cGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAaga                      >  2:2308109/1‑150 (MQ=255)

                                                                                                                                    ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTc                   >  1:5175863/1‑150 (MQ=255)

                                                                                                                                    ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTc                   >  1:562938/1‑150 (MQ=255)

                                                                                                                                     tCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCt                  <  1:1345090/150‑1 (MQ=255)

                                                                                                                                      cTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCtt                 <  1:632856/150‑1 (MQ=255)

                                                                                                                                      cTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCtt                 <  1:2242010/150‑1 (MQ=255)

                                                                                                                                       tGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTa                <  1:2899183/150‑1 (MQ=255)

                                                                                                                                         gATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTAct              >  1:905926/1‑150 (MQ=255)

                                                                                                                                              ttGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCg         >  1:50508/1‑150 (MQ=255)

                                                                                                                                                gTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCggg       <  2:1497295/150‑1 (MQ=255)

                                                                                                                                                 ttAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGggc      <  2:950392/150‑1 (MQ=255)

                                                                                                                                                  tAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGggcg     <  2:5078258/150‑1 (MQ=255)

                                                                                                                                                    aGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGggcggc   <  1:1991111/150‑1 (MQ=255)

CGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTG‑TTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCT  >  CP009273/4402464‑4402762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`