Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,422,157 C→A 8.1% A239A (GCG→GCT)  ytfF ← DMT transporter family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,422,1570CA 8.1% 1410.6 / 61.6 387A239A (GCG→GCT) ytfFDMT transporter family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (188/167);  new base A (18/14);  total (206/181)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

AGCGGCGGCATTTGCTGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCAT  >  CP009273/4422008‑4422306
                                                                                                                                                     |                                                                                                                                                     
aGCGGCGGCATTTGCTGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATc                                                                                                                                                       <  1:4711127/150‑1 (MQ=255)
        cATTTGCTGGCGGAGTAAAAAAGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCAt                                                                                                                                               <  2:4274829/150‑1 (MQ=255)
         aTTTGCTGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATc                                                                                                                                              >  1:1403514/1‑150 (MQ=255)
              cTGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACt                                                                                                                                         <  1:1802528/150‑1 (MQ=255)
               tGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACta                                                                                                                                        <  2:3043304/150‑1 (MQ=255)
               tGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACta                                                                                                                                        >  1:2300572/1‑150 (MQ=255)
                  cGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTAGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACtaata                                                                                                                                     <  2:1261510/150‑1 (MQ=255)
                   ggAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACtaataa                                                                                                                                    <  1:4276159/150‑1 (MQ=255)
                    gAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATaaa                                                                                                                                   <  1:1426018/150‑1 (MQ=255)
                     aGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAac                                                                                                                                  >  2:2980288/1‑150 (MQ=255)
                        aaaaaGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACAcc                                                                                                                               >  1:3448028/1‑150 (MQ=255)
                          aaaGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAg                                                                                                                             >  2:2622088/1‑150 (MQ=255)
                           aaGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGc                                                                                                                            <  2:3266397/150‑1 (MQ=255)
                            aGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCg                                                                                                                           <  2:2549774/150‑1 (MQ=255)
                              gtgtACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATAAACACCAGCGGa                                                                                                                         <  1:3592400/150‑1 (MQ=255)
                                gtACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACg                                                                                                                       >  1:4936135/1‑150 (MQ=255)
                                 tACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACgg                                                                                                                      >  2:2192266/1‑150 (MQ=255)
                                  aCAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACggg                                                                                                                     >  2:5001426/1‑150 (MQ=255)
                                    aGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACggggg                                                                                                                   >  2:1138888/1‑150 (MQ=255)
                                     gCAAACCTGCCAGCGTTTCGAAAACAATCATCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGc                                                                                                                  <  2:5113130/150‑1 (MQ=255)
                                       aaaCCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCACCCATCAGACTAATAAACACCAGCGGACGGGGGCCa                                                                                                                >  1:2658661/1‑150 (MQ=255)
                                        aaCCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCaa                                                                                                               >  2:2061544/1‑150 (MQ=255)
                                              ccAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTa                                                                                                         <  1:4454892/150‑1 (MQ=255)
                                               cAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAg                                                                                                        >  1:3432508/1‑150 (MQ=255)
                                               cAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAg                                                                                                        <  1:1478378/150‑1 (MQ=255)
                                                aGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAgg                                                                                                       >  1:874967/1‑150 (MQ=255)
                                                  cGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGga                                                                                                     <  1:2278801/150‑1 (MQ=255)
                                                  cGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGga                                                                                                     >  1:5147604/1‑150 (MQ=255)
                                                   gTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGgag                                                                                                    >  2:423985/1‑150 (MQ=255)
                                                    tttCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGgaga                                                                                                   <  1:3848394/150‑1 (MQ=255)
                                                    tttCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAAGAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGgaga                                                                                                   <  2:2876139/150‑1 (MQ=255)
                                                     ttCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGaa                                                                                                  <  1:716509/150‑1 (MQ=255)
                                                     tacGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCACCCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGaa                                                                                                  >  2:77141/3‑150 (MQ=255)
                                                      tCGAAAACAATGAGCGGCCCGAGAATCTCTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAg                                                                                                 <  1:5182154/150‑1 (MQ=255)
                                                        gAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTc                                                                                               <  1:2802133/150‑1 (MQ=255)
                                                         aaaaCAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTcc                                                                                              >  1:4240947/1‑150 (MQ=255)
                                                           aaCAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTCATAAACACCAGCGGACGGGGGCCCAAGGGTAGGGAGAAGTCCgg                                                                                            >  1:4992080/1‑150 (MQ=255)
                                                               aTCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGtt                                                                                        >  2:4724387/1‑150 (MQ=255)
                                                                  aGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGc                                                                                     >  1:1178733/1‑150 (MQ=255)
                                                                     ggCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGACGTCCGGCGTTTGCGTa                                                                                  >  1:1667313/1‑150 (MQ=255)
                                                                     ggCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTa                                                                                  <  1:2722585/150‑1 (MQ=255)
                                                                      gCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTAt                                                                                 <  2:4500160/150‑1 (MQ=255)
                                                                         cGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCa                                                                              >  1:1004515/1‑150 (MQ=255)
                                                                          gagaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAg                                                                             >  2:4326864/1‑150 (MQ=255)
                                                                          gagaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAg                                                                             >  1:3023927/1‑150 (MQ=255)
                                                                          gagaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAg                                                                             >  1:80448/1‑150 (MQ=255)
                                                                           agaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGc                                                                            <  2:1668159/150‑1 (MQ=255)
                                                                           agaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGc                                                                            <  2:3641188/150‑1 (MQ=255)
                                                                            gaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGcc                                                                           <  1:5035416/150‑1 (MQ=255)
                                                                            gaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGcc                                                                           >  2:688253/1‑150 (MQ=255)
                                                                             aaTCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCa                                                                          <  1:3266709/150‑1 (MQ=255)
                                                                              aTCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCCGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAg                                                                         <  2:4011789/150‑1 (MQ=255)
                                                                                  cTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAAc                                                                     >  1:1243230/1‑150 (MQ=255)
                                                                                   tGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACa                                                                    >  2:4562484/1‑150 (MQ=255)
                                                                                    gTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACaa                                                                   >  2:1075389/1‑150 (MQ=255)
                                                                                      cGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGc                                                                 <  1:2380126/150‑1 (MQ=255)
                                                                                        gTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGcga                                                               >  2:4077053/1‑150 (MQ=255)
                                                                                           aTAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGcgacga                                                            >  1:642463/1‑150 (MQ=255)
                                                                                             aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACgaga                                                          >  2:2015567/1‑150 (MQ=255)
                                                                                             aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACgaga                                                          >  1:3080518/1‑150 (MQ=255)
                                                                                                tggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAATGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAg                                                       >  2:1960208/1‑150 (MQ=255)
                                                                                                tggctggcGACGTTCCAGCAGAGTGAGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATAAACACCAGCGGAGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTGTGCGTATTCAGCCAGTAACAGGCGACGAGATAg                                                       >  2:3239873/1‑150 (MQ=255)
                                                                                                 ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGc                                                      >  2:134576/1‑150 (MQ=255)
                                                                                                  gctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGcc                                                     >  2:3585768/1‑150 (MQ=255)
                                                                                                    tggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGa                                                   >  1:958461/1‑150 (MQ=255)
                                                                                                     ggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGAt                                                  >  1:4348575/1‑150 (MQ=255)
                                                                                                     ggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGAt                                                  >  1:2203772/1‑150 (MQ=255)
                                                                                                          cGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTg                                             >  1:2975080/1‑150 (MQ=255)
                                                                                                          cGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTg                                             >  2:907581/1‑150 (MQ=255)
                                                                                                          cGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTg                                             >  1:338132/1‑150 (MQ=255)
                                                                                                           gTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGa                                            <  1:1873176/150‑1 (MQ=255)
                                                                                                           gTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATAGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGa                                            >  2:1822534/1‑150 (MQ=255)
                                                                                                            ttCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGaa                                           <  2:1302257/150‑1 (MQ=255)
                                                                                                             tCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCATACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGATAAGTCCGGCGTTTGCGTATTCAGCAAGTAACAAGCGACGAGATAGCCGATAAGTGaaa                                          <  1:62511/150‑1 (MQ=255)
                                                                                                               cagcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTCGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAAcc                                        >  2:3542647/1‑150 (MQ=255)
                                                                                                                agcatAGTGCGTCAACCCATGAGCAAAGCACGTCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCg                                       <  2:2816541/150‑1 (MQ=255)
                                                                                                                   agagTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCa                                    >  1:2625523/1‑150 (MQ=255)
                                                                                                                   agagTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCa                                    >  1:5214356/1‑150 (MQ=255)
                                                                                                                       tGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCGAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAgtgt                                <  1:1907025/150‑1 (MQ=255)
                                                                                                                         cgcCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGa                              <  1:4699645/150‑1 (MQ=255)
                                                                                                                            cAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCa                           >  2:4362427/1‑150 (MQ=255)
                                                                                                                             aaCCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAg                          >  2:3268911/1‑150 (MQ=255)
                                                                                                                             aaCCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAg                          >  2:3944981/1‑150 (MQ=255)
                                                                                                                             aaCCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAg                          >  2:5236207/1‑150 (MQ=255)
                                                                                                                                  aTGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACCCCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCt                     <  2:3878119/150‑1 (MQ=255)
                                                                                                                                    gAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTgc                   >  2:2477409/1‑150 (MQ=255)
                                                                                                                                        aaaGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTg               >  1:3705947/1‑150 (MQ=255)
                                                                                                                                        aaaGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTg               <  2:3421723/150‑1 (MQ=255)
                                                                                                                                        aaaGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTg               <  2:797617/150‑1 (MQ=255)
                                                                                                                                          aGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGtt             >  1:4023660/1‑150 (MQ=255)
                                                                                                                                           gCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTg            <  2:269250/150‑1 (MQ=255)
                                                                                                                                           gCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTg            <  1:2540768/150‑1 (MQ=255)
                                                                                                                                              cGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGccc         <  1:3683800/150‑1 (MQ=255)
                                                                                                                                               ggCTATAGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCca        <  1:4581527/150‑1 (MQ=255)
                                                                                                                                                 cTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCcaca      <  2:4441428/150‑1 (MQ=255)
                                                                                                                                                 cTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCcaca      >  2:2739972/1‑150 (MQ=255)
                                                                                                                                                 cTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCcaca      >  2:2372555/1‑150 (MQ=255)
                                                                                                                                                    tCGCAACCATCAGACTAATAAACACCCGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCAcatca   <  2:1616338/150‑1 (MQ=255)
                                                                                                                                                    tCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCAcatca   <  1:827769/150‑1 (MQ=255)
                                                                                                                                                     cGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCAcatcat  >  1:205254/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGGCGGCATTTGCTGGCGGAGTAAAAAGGTGTACAGCAAACCTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCAT  >  CP009273/4422008‑4422306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑