Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,609 G→T 70.4% D390Y (GAT→TAT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,6090GT70.4% 407.2 / 308.8 304D390Y (GAT→TAT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base G (32/58);  new base T (105/108);  total (138/166)
Fisher's exact test for biased strand distribution p-value = 3.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 307 total aligned reads displayed.

CTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAA  >  CP009273/4458462‑4458758
                                                                                                                                                   |                                                                                                                                                     
cTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTat                                                                                                                                                     <  2:2402734/150‑1 (MQ=255)
      cgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTcc                                                                                                                                               <  2:1577736/150‑1 (MQ=255)
        cggcgCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCgg                                                                                                                                             <  1:2496367/150‑1 (MQ=255)
          gcgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTa                                                                                                                                           <  1:1934074/150‑1 (MQ=255)
           cgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaa                                                                                                                                          <  1:4219864/150‑1 (MQ=255)
             cAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaaaa                                                                                                                                        >  1:3003469/1‑150 (MQ=255)
             cAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaaaa                                                                                                                                        >  1:2986702/1‑150 (MQ=255)
               gTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAAcc                                                                                                                                      >  1:3126486/1‑150 (MQ=255)
                 aaaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                                    >  2:627553/1‑150 (MQ=255)
                 aaaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                                    <  2:3501298/150‑1 (MQ=255)
                  aaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGc                                                                                                                                   <  1:2128242/150‑1 (MQ=255)
                      tGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAc                                                                                                                               <  1:2016480/150‑1 (MQ=255)
                      tGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAc                                                                                                                               <  2:4394718/150‑1 (MQ=255)
                      tGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGAAATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAAc                                                                                                                               <  2:4814735/150‑1 (MQ=255)
                             aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgct                                                                                                                        <  1:507955/150‑1 (MQ=255)
                              cAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgctg                                                                                                                       <  2:2003018/150‑1 (MQ=255)
                              cAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgctg                                                                                                                       <  2:4606523/150‑1 (MQ=255)
                              cAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgctg                                                                                                                       <  2:4482870/150‑1 (MQ=255)
                                 ataGTGTTCGTCAAACATCTGGTTGCTATTCAGAACTTTTGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCAt                                                                                                                    <  2:3436579/148‑1 (MQ=255)
                                  aaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATa                                                                                                                   <  2:1630781/150‑1 (MQ=255)
                                   aGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAg                                                                                                                  <  1:1174832/150‑1 (MQ=255)
                                    gTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCTCAATGGATATTCTGTGCACTGAAAAAACCGGCACCCTGACGAACGAAACAATTGTGCTGGAGAATCCTACCAATATCTCCGGTAAAAACAGCGAACGCGAGCTGCATAgc                                                                                                                 >  2:4113531/1‑150 (MQ=255)
                                     tGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAgcg                                                                                                                >  2:3107238/1‑150 (MQ=255)
                                       aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGcgcg                                                                                                              >  2:61022/1‑150 (MQ=255)
                                       aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGcgcg                                                                                                              >  2:1846606/1‑150 (MQ=255)
                                       aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGcgcg                                                                                                              >  1:476232/1‑150 (MQ=255)
                                         cGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTg                                                                                                            <  1:2682804/150‑1 (MQ=255)
                                         cGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTg                                                                                                            <  1:4238125/150‑1 (MQ=255)
                                           tCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGt                                                                                                          <  1:441094/150‑1 (MQ=255)
                                            cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGtt                                                                                                         <  1:3726579/150‑1 (MQ=255)
                                            cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGtt                                                                                                         <  1:3726391/150‑1 (MQ=255)
                                            cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGtt                                                                                                         <  1:429886/150‑1 (MQ=255)
                                             aaaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTg                                                                                                        >  2:3255200/1‑150 (MQ=255)
                                               ccATCTGGATGCTCTTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGaa                                                                                                      <  2:3637444/149‑1 (MQ=255)
                                               aCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGaa                                                                                                      <  2:125307/150‑1 (MQ=255)
                                               aCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGaa                                                                                                      <  2:1015935/150‑1 (MQ=255)
                                               aCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGaa                                                                                                      <  2:823743/150‑1 (MQ=255)
                                                cATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAAc                                                                                                     >  1:5228282/1‑150 (MQ=255)
                                                   cTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGt                                                                                                  >  1:4981387/1‑150 (MQ=255)
                                                     ggttGCTTTTCATAACTTTGGCGCAATGGTATTTCTGTGCACAGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCa                                                                                                <  2:2570741/147‑1 (MQ=255)
                                                     ggATGCTAGTCAGAACTTTGGCGCAATGGATAGTCTGTGCACTGATACCACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCa                                                                                                <  2:2136525/150‑1 (MQ=255)
                                                      gATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAt                                                                                               >  1:3914073/1‑150 (MQ=255)
                                                      gATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAt                                                                                               <  2:11704/150‑1 (MQ=255)
                                                       aTGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCAtt                                                                                              <  2:3016913/150‑1 (MQ=255)
                                                         gCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTAt                                                                                            <  2:5080070/150‑1 (MQ=255)
                                                         gCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTAt                                                                                            <  2:4971739/150‑1 (MQ=255)
                                                          cTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATc                                                                                           <  2:4746525/150‑1 (MQ=255)
                                                           tATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCa                                                                                          <  1:3809241/150‑1 (MQ=255)
                                                            aTTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAg                                                                                         <  1:4526968/150‑1 (MQ=255)
                                                               cAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAACACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGAcc                                                                                      <  2:1195512/150‑1 (MQ=255)
                                                                 gAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGAGCAGCAATGCGCGTGGTTGAACAGACATTATCAGAACgg                                                                                    >  2:2945425/1‑150 (MQ=255)
                                                                  aaCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                                   >  1:3765347/1‑150 (MQ=255)
                                                                   aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGTATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                                  >  2:2738482/1‑150 (MQ=255)
                                                                   aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                                  >  1:1001053/1‑150 (MQ=255)
                                                                   aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                                  <  1:1729806/150‑1 (MQ=255)
                                                                    cTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACt                                                                                 <  1:2253594/150‑1 (MQ=255)
                                                                      ttGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTa                                                                               <  1:287011/150‑1 (MQ=255)
                                                                        ggCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaaa                                                                             <  2:4903313/150‑1 (MQ=255)
                                                                        ggCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaaa                                                                             >  1:1291673/1‑150 (MQ=255)
                                                                          cgcAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaaaaa                                                                           >  1:3239738/1‑150 (MQ=255)
                                                                               tGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGc                                                                      >  2:4232034/1‑150 (MQ=255)
                                                                                ggATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAATTTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCt                                                                     <  2:2487300/150‑1 (MQ=255)
                                                                                ggATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCt                                                                     >  1:4660065/1‑150 (MQ=255)
                                                                                ggATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCt                                                                     >  1:3060793/1‑150 (MQ=255)
                                                                                 gATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTc                                                                    >  2:1898894/1‑150 (MQ=255)
                                                                                 gATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTc                                                                    >  2:4275676/1‑150 (MQ=255)
                                                                                  atatTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCg                                                                   >  2:4137049/1‑150 (MQ=255)
                                                                                        tgtgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAg                                                             <  1:3109491/150‑1 (MQ=255)
                                                                                         gtgCACTGATAAAACCGGTACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGc                                                            >  2:1449141/1‑150 (MQ=255)
                                                                                         gtgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGc                                                            <  1:4507296/150‑1 (MQ=255)
                                                                                          tgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCg                                                           >  1:1260119/1‑150 (MQ=255)
                                                                                            cACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGt                                                         <  2:204225/150‑1 (MQ=255)
                                                                                             aCTGATAAAACAGGCACCCTTACGCATGATAAAATTGTGCTGTAGAATCATACCTATATCTCCGTTTAAAACAGCGATAGCTTGCTGGTTAGGGCGTGGTTGAACAGTCTTTATCAGACCGGACTTAAAATCATTCTCGATAATGCGGTg                                                        <  2:3422713/150‑1 (MQ=255)
                                                                                              cTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                       >  2:3378917/1‑150 (MQ=255)
                                                                                              cTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                       >  1:3276984/1‑150 (MQ=255)
                                                                                               tGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCt                                                      <  1:4005359/150‑1 (MQ=255)
                                                                                                gATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTc                                                     >  2:131887/1‑150 (MQ=255)
                                                                                                    aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAg                                                 <  2:2963761/150‑1 (MQ=255)
                                                                                                       ccGGCACCCTGACTCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGACCGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTa                                              <  2:4008619/150‑1 (MQ=255)
                                                                                                       ccGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTa                                              <  2:1579094/150‑1 (MQ=255)
                                                                                                        cGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTAc                                             >  2:4744358/1‑150 (MQ=255)
                                                                                                              ccTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGa                                       <  1:808983/150‑1 (MQ=255)
                                                                                                              ccTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGa                                       <  1:1712835/150‑1 (MQ=255)
                                                                                                               cTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGaa                                      >  1:1770116/1‑150 (MQ=255)
                                                                                                                tGACGCAGGATAAAAGTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAag                                     >  2:4211384/1‑150 (MQ=255)
                                                                                                                     cAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                                <  1:3983032/150‑1 (MQ=255)
                                                                                                                       ggATAAAATTGTGATGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                              <  2:392527/150‑1 (MQ=255)
                                                                                                                            aaaTTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgct                         >  1:1120939/1‑150 (MQ=255)
                                                                                                                                 gtgCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTg                    >  2:5057270/1‑150 (MQ=255)
                                                                                                                                     tGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCa                <  1:634289/150‑1 (MQ=255)
                                                                                                                                      ggAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAg               <  2:2607399/150‑1 (MQ=255)
                                                                                                                                       gagaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGt              <  1:1502659/150‑1 (MQ=255)
                                                                                                                                       gagaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGt              >  2:525422/1‑150 (MQ=255)
                                                                                                                                             cATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTgg        <  2:3683356/150‑1 (MQ=255)
                                                                                                                                             cATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTgg        <  2:1064025/150‑1 (MQ=255)
                                                                                                                                              aTACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGc       >  1:4105386/1‑150 (MQ=255)
                                                                                                                                               tACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCa      >  2:2316695/1‑150 (MQ=255)
                                                                                                                                                aCCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCaa     >  1:3398398/1‑150 (MQ=255)
                                                                                                                                                   gATATCTCCGGTAAAACCAGCGAACGCTTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCgaaaa  >  2:723969/1‑150 (MQ=255)
                                                                                                                                                   gATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCaaaaa  >  1:2397370/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAA  >  CP009273/4458462‑4458758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑