BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,466,937	A→C	5.4%	T562P (ACT→CCT)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,466,937	0	A	C	5.4%	1041.5 / 41.4	323	T562P (ACT→CCT)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (162/142); new base C (10/8); total (172/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 327 total aligned reads displayed.

AGGGTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGA  >  CP009273/4466788‑4467086

aGGGTGAAAAGATTTATTTAGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCaaa                                                                                                                                                       <  2:2618793/150‑1 (MQ=255)

  ggTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACt                                                                                                                                                     >  2:2989286/1‑150 (MQ=255)

   gTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTa                                                                                                                                                    >  1:684860/1‑150 (MQ=255)

    tGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTAt                                                                                                                                                   <  1:4498396/150‑1 (MQ=255)

      aaaaGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCt                                                                                                                                                 >  1:4365765/1‑150 (MQ=255)

           atttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCaa                                                                                                                                            >  1:4580333/1‑150 (MQ=255)

            tttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAAt                                                                                                                                           <  2:4689484/150‑1 (MQ=255)

             ttatttTTATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATc                                                                                                                                          <  1:3547037/150‑1 (MQ=255)

             ttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATc                                                                                                                                          <  2:1869057/150‑1 (MQ=255)

                 tttGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCgaga                                                                                                                                      >  1:3881346/1‑150 (MQ=255)

                    gATAATTTAATATATGATTTAAATGATAATGATAAGGAATATAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGtt                                                                                                                                   <  1:3807592/150‑1 (MQ=255)

                     aTAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTa                                                                                                                                  <  1:2375688/150‑1 (MQ=255)

                      tAATTTAAAATATGATTTAAATAATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTaa                                                                                                                                 >  1:5003109/1‑150 (MQ=255)

                       aaTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAATGAAAAACTGAATAATGAATACAAACTTAGAGTTAGAATGATGACAACTATCTTGCAATCGAGAGTTAAt                                                                                                                                >  2:3392539/1‑150 (MQ=255)

                       aaTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAAt                                                                                                                                <  2:103214/150‑1 (MQ=255)

                       aaTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAAt                                                                                                                                <  2:1654280/150‑1 (MQ=255)

                        aTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATg                                                                                                                               >  2:3477075/1‑150 (MQ=255)

                         tttAAAATATTATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGt                                                                                                                              <  2:3603769/150‑1 (MQ=255)

                         tttAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGt                                                                                                                              >  1:2597487/1‑150 (MQ=255)

                          ttAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTa                                                                                                                             >  1:1513941/1‑150 (MQ=255)

                               atatGATTAAAATGATAATGATAAGGAATCTAATAAAGAAATATTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAAAACAAACTTAGATTTAGAATGATGCAAACAATCTAGCAATCGAGAGTTAAAGTATTAca                                                                                                                        >  2:4299541/1‑149 (MQ=255)

                                tatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCa                                                                                                                       <  2:1582743/150‑1 (MQ=255)

                                   gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatat                                                                                                                    <  1:4805715/150‑1 (MQ=255)

                                   gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatat                                                                                                                    <  1:445136/150‑1 (MQ=255)

                                   gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatat                                                                                                                    <  2:2517230/150‑1 (MQ=255)

                                    aTTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatata                                                                                                                   <  2:2403432/150‑1 (MQ=255)

                                      ttAAATTATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATAtt                                                                                                                 <  2:4112060/150‑1 (MQ=255)

                                      ttAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATAtt                                                                                                                 <  1:4566293/150‑1 (MQ=255)

                                      ttAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAATTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACAatataat                                                                                                                 >  2:1672452/1‑148 (MQ=255)

                                       tAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTa                                                                                                                >  1:4890026/1‑150 (MQ=255)

                                       tAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTa                                                                                                                >  1:1698616/1‑150 (MQ=255)

                                             ataattataaGGAATCTAATAAAGAAATTTTGAAGAATCAAACAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAAc                                                                                                          <  2:4927891/150‑1 (MQ=255)

                                              taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACa                                                                                                         <  2:619388/150‑1 (MQ=255)

                                                atgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGc                                                                                                       >  1:2898501/1‑150 (MQ=255)

                                                 tgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCg                                                                                                      <  2:543235/150‑1 (MQ=255)

                                                   ataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGtt                                                                                                    >  2:313088/1‑150 (MQ=255)

                                                     aaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTta                                                                                                  >  2:2033531/1‑150 (MQ=255)

                                                      aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaa                                                                                                 <  2:293477/150‑1 (MQ=255)

                                                         aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaata                                                                                              >  1:1992321/1‑150 (MQ=255)

                                                         aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaata                                                                                              <  1:3365400/150‑1 (MQ=255)

                                                          gtCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaataa                                                                                             >  2:1129643/2‑150 (MQ=255)

                                                            cTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAAc                                                                                           <  1:2989286/150‑1 (MQ=255)

                                                            cTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAAc                                                                                           <  2:3704646/150‑1 (MQ=255)

                                                            cTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAAc                                                                                           <  1:856321/150‑1 (MQ=255)

                                                             taataaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACt                                                                                          <  1:3080193/150‑1 (MQ=255)

                                                              aataaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTa                                                                                         >  1:1916161/1‑150 (MQ=255)

                                                               ataaAGAAATTTTGAAGAAACAACCAGCTAATGTTATCAAAGAAAAACTGAATAATGAATACACACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTaa                                                                                        <  2:1288730/150‑1 (MQ=255)

                                                                taaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTaaa                                                                                       >  1:955431/1‑150 (MQ=255)

                                                                  aaGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATc                                                                                     <  2:4066017/150‑1 (MQ=255)

                                                                  aaGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATc                                                                                     <  1:1527812/150‑1 (MQ=255)

                                                                  aaGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATc                                                                                     >  1:902292/1‑150 (MQ=255)

                                                                     aaaTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAc                                                                                  <  1:3685657/150‑1 (MQ=255)

                                                                      aaTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAcc                                                                                 >  1:1021580/1‑150 (MQ=255)

                                                                             aagaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAt                                                                          <  2:819159/150‑1 (MQ=255)

                                                                                aaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTa                                                                       <  2:706020/150‑1 (MQ=255)

                                                                                 aTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTAc                                                                      >  1:5116383/1‑150 (MQ=255)

                                                                                  tCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACg                                                                     <  1:1804313/150‑1 (MQ=255)

                                                                                     aCCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGtat                                                                  >  2:3353820/1‑150 (MQ=255)

                                                                                      ccAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGtata                                                                 <  1:4047016/150‑1 (MQ=255)

                                                                                       cAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAAAGTATTACCATATATTAATGAACAGCGGTTAAATAAACTCAATCCACCGGAAAATTTACGTATAg                                                                >  2:2977863/1‑150 (MQ=255)

                                                                                        aGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGc                                                               >  1:3942466/1‑150 (MQ=255)

                                                                                           tAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                            <  2:39779/150‑1 (MQ=255)

                                                                                            aaTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATTGAAATa                                                           >  2:4453474/1‑150 (MQ=255)

                                                                                             aTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAg                                                          <  1:1944924/150‑1 (MQ=255)

                                                                                              tGTTATCAAAGAAAAACTGAATAATGAATACAATCTTAGATTTAGAAAGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATAAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATGTACGTATAGCAAAAGa                                                         >  2:4796258/1‑150 (MQ=255)

                                                                                              tGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGa                                                         <  1:319433/150‑1 (MQ=255)

                                                                                              tGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGa                                                         >  2:1927195/1‑150 (MQ=255)

                                                                                                 tATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAaca                                                      <  2:2328326/150‑1 (MQ=255)

                                                                                                   tCAAAGAAAAACTTAATAATGAATACAAACTTAGATTTAGAATGATGCAATCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACt                                                    <  1:2700505/150‑1 (MQ=255)

                                                                                                    cAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACtt                                                   <  2:4890026/150‑1 (MQ=255)

                                                                                                    cAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAAAAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACtg                                                   >  2:4263336/1‑149 (MQ=255)

                                                                                                     aaaGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTTTATTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCTCCGGAAAATTTACGTATAGCAATAGAACACttt                                                  <  1:2050537/150‑1 (MQ=255)

                                                                                                     aaaGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACttt                                                  <  1:22502/150‑1 (MQ=255)

                                                                                                        gAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTggg                                               >  1:2427433/1‑150 (MQ=255)

                                                                                                         aaaaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGt                                              >  1:4273112/1‑150 (MQ=255)

                                                                                                         aaaaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGt                                              >  2:4846659/1‑150 (MQ=255)

                                                                                                         aaaaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGt                                              >  1:544259/1‑150 (MQ=255)

                                                                                                           aaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTgg                                            <  2:1424673/150‑1 (MQ=255)

                                                                                                             aCTGAATAATGAATACAACCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaa                                          <  1:1403125/150‑1 (MQ=255)

                                                                                                               tGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaaga                                        <  2:4368202/150‑1 (MQ=255)

                                                                                                                 aataatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAAt                                      >  1:480865/1‑150 (MQ=255)

                                                                                                                        aaTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAATATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTa                               <  2:2472114/150‑1 (MQ=255)

                                                                                                                        aaTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTa                               <  2:5070477/150‑1 (MQ=255)

                                                                                                                         aTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGAAATAGAACACTTTGGGTGGAAGAATAGACCTAt                              >  2:3742023/1‑150 (MQ=255)

                                                                                                                           aCAAAATTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCa                            <  2:2216185/150‑1 (MQ=255)

                                                                                                                            cAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTAAAAAAAACAAAATACAACGGAAAATTTTCGTATTTAAATAATAAACTTTTGGTGGTTGAATAGAACTATGAc                           >  2:2822654/1‑150 (MQ=255)

                                                                                                                             aaaCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACt                          <  1:361477/150‑1 (MQ=255)

                                                                                                                             aaaCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACt                          >  1:4482949/1‑150 (MQ=255)

                                                                                                                             aaaCTTAGATTGAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATTTTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTTTAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACt                          <  2:5178560/150‑1 (MQ=255)

                                                                                                                               aCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGc                        >  2:3256565/1‑150 (MQ=255)

                                                                                                                                  tAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATa                     <  1:1561873/150‑1 (MQ=255)

                                                                                                                                  tAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATa                     <  1:149114/150‑1 (MQ=255)

                                                                                                                                  tAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATa                     <  1:4714363/150‑1 (MQ=255)

                                                                                                                                   aGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaa                    <  2:1837910/150‑1 (MQ=255)

                                                                                                                                     aTTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaaaa                  <  1:1476529/150‑1 (MQ=255)

                                                                                                                                      tttAGAATGATGCAAACTATCTTGAAATCGAGAGTTAATGTATTACAATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGAAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGAATAAaat                 >  2:2089927/1‑150 (MQ=255)

                                                                                                                                        tAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaataa               <  1:2362679/150‑1 (MQ=255)

                                                                                                                                           aaTGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt            >  1:3130835/1‑150 (MQ=255)

                                                                                                                                            atgatgCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg           <  1:1535777/150‑1 (MQ=255)

                                                                                                                                             tgatgCAAACTATCTTGCAATCGAGAATTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCACTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGa          >  1:3491188/1‑150 (MQ=255)

AGGGTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGA  >  CP009273/4466788‑4467086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`