Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,489,542 A→T 19.1% pseudogene (810/942 nt) yjgX ← pseudogene fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,489,5420AT19.1% 724.3 / 150.7 279pseudogene (810/942 nt)yjgXpseudogene fragment
Reads supporting (aligned to +/- strand):  ref base A (100/125);  new base T (23/31);  total (123/156)
Fisher's exact test for biased strand distribution p-value = 8.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

AGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATT  >  CP009273/4489394‑4489691
                                                                                                                                                    |                                                                                                                                                     
 gTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACa                                                                                                                                                     >  2:3786614/1‑150 (MQ=255)
  tCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCaa                                                                                                                                                    >  2:4813204/1‑150 (MQ=255)
   cTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACaaa                                                                                                                                                   <  2:2621834/150‑1 (MQ=255)
    tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAAt                                                                                                                                                  <  1:692571/150‑1 (MQ=255)
    tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAAt                                                                                                                                                  <  1:2646896/150‑1 (MQ=255)
     gcaatatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATa                                                                                                                                                 <  1:3949417/147‑1 (MQ=255)
       cATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATAcc                                                                                                                                               <  1:3358861/150‑1 (MQ=255)
        atatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCg                                                                                                                                              <  1:2958606/150‑1 (MQ=255)
        atatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCg                                                                                                                                              >  1:1316284/1‑150 (MQ=255)
        atatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCg                                                                                                                                              <  1:3208823/150‑1 (MQ=255)
         tatatGATTACATGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCgg                                                                                                                                             <  2:2852984/150‑1 (MQ=255)
          atatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGt                                                                                                                                            >  1:2767381/1‑150 (MQ=255)
           tatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTc                                                                                                                                           >  1:1224743/1‑150 (MQ=255)
            atgatgACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCa                                                                                                                                          <  2:5106180/150‑1 (MQ=255)
             tGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCAt                                                                                                                                         >  2:551734/1‑150 (MQ=255)
             tGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCAt                                                                                                                                         >  2:4202550/1‑150 (MQ=255)
              gATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATc                                                                                                                                        <  2:3898233/150‑1 (MQ=255)
              gATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATc                                                                                                                                        >  2:2067066/1‑150 (MQ=255)
               aTTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATcc                                                                                                                                       >  2:2194883/1‑150 (MQ=255)
                   ccTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTg                                                                                                                                   <  1:642634/150‑1 (MQ=255)
                    cTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGa                                                                                                                                  >  2:558798/1‑150 (MQ=255)
                    cTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGa                                                                                                                                  <  1:1785513/150‑1 (MQ=255)
                     tgtgATCAGGAAGAATAAGTGTCTGAGGTTTCAATCTGTCTGCCGGTATATTCCTATCTCCCATAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGaa                                                                                                                                 <  2:3110374/150‑1 (MQ=255)
                      gtgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGaaa                                                                                                                                <  1:1873937/150‑1 (MQ=255)
                      gtgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGaaa                                                                                                                                >  2:1685357/1‑150 (MQ=255)
                       tgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGaaaa                                                                                                                               >  2:142609/1‑150 (MQ=255)
                         aTCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAAt                                                                                                                             <  1:1617601/150‑1 (MQ=255)
                          tCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATa                                                                                                                            <  1:2372131/150‑1 (MQ=255)
                          tCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATa                                                                                                                            >  1:569964/1‑150 (MQ=255)
                           cAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATAc                                                                                                                           <  2:1575475/150‑1 (MQ=255)
                           cAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATAc                                                                                                                           <  2:4544308/150‑1 (MQ=255)
                                agaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCg                                                                                                                      >  2:3582485/1‑150 (MQ=255)
                                   aTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGaaa                                                                                                                   >  2:1279820/1‑150 (MQ=255)
                                    tAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAAt                                                                                                                  <  1:1369881/150‑1 (MQ=255)
                                    tAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAAt                                                                                                                  <  1:4870147/150‑1 (MQ=255)
                                     aaGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATg                                                                                                                 >  1:5176683/1‑150 (MQ=255)
                                      aGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGa                                                                                                                >  1:1023038/1‑150 (MQ=255)
                                         ggCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAGTGGCgg                                                                                                             >  1:4026952/1‑150 (MQ=255)
                                           cTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGc                                                                                                           <  1:1812468/150‑1 (MQ=255)
                                            tGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCa                                                                                                          <  2:195444/150‑1 (MQ=255)
                                              aGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACt                                                                                                        >  1:3894625/1‑150 (MQ=255)
                                                  ttCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAtt                                                                                                    >  2:1818856/1‑150 (MQ=255)
                                                     aaaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAttttt                                                                                                 >  1:4360001/1‑150 (MQ=255)
                                                      aaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTc                                                                                                >  2:3192552/1‑150 (MQ=255)
                                                         tGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTAc                                                                                             >  1:1421967/1‑150 (MQ=255)
                                                         tGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTAc                                                                                             <  1:2420355/150‑1 (MQ=255)
                                                                 ggTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGc                                                                                     <  1:3034848/150‑1 (MQ=255)
                                                                  gTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCt                                                                                    <  1:4710802/150‑1 (MQ=255)
                                                                  gTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCt                                                                                    <  1:4987910/150‑1 (MQ=255)
                                                                  gTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCt                                                                                    <  2:1131221/150‑1 (MQ=255)
                                                                    atatTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtga                                                                                  <  2:1043857/150‑1 (MQ=255)
                                                                     tatTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgat                                                                                 <  1:4649710/150‑1 (MQ=255)
                                                                        tCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATgag                                                                              >  2:2392517/1‑150 (MQ=255)
                                                                         ccTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATgaga                                                                             >  1:756513/1‑150 (MQ=255)
                                                                            ctctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGt                                                                          >  2:4699658/1‑150 (MQ=255)
                                                                             tctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGtt                                                                         <  1:3185807/150‑1 (MQ=255)
                                                                              ctcCCTTAAAAACCATAACCGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTa                                                                        <  1:3832086/150‑1 (MQ=255)
                                                                               tcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTAt                                                                       <  2:3016074/150‑1 (MQ=255)
                                                                                 ccTTAACATCCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGt                                                                     <  2:3312784/150‑1 (MQ=255)
                                                                                 ccTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGt                                                                     <  2:686229/150‑1 (MQ=255)
                                                                                    tAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCAc                                                                  <  2:3468463/150‑1 (MQ=255)
                                                                                      aaaaCCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTg                                                                >  1:4899843/1‑150 (MQ=255)
                                                                                        aaCCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGc                                                              >  2:3643637/1‑150 (MQ=255)
                                                                                        aaCCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGc                                                              <  1:4511377/150‑1 (MQ=255)
                                                                                         aCCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCg                                                             >  1:4945030/1‑150 (MQ=255)
                                                                                          ccATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCgg                                                            >  2:4261831/1‑150 (MQ=255)
                                                                                           cATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGt                                                           >  1:2306917/1‑150 (MQ=255)
                                                                                           cATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGt                                                           >  1:2306659/1‑150 (MQ=255)
                                                                                           cATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGt                                                           >  2:4741991/1‑150 (MQ=255)
                                                                                            aTAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGta                                                          >  1:1489551/1‑150 (MQ=255)
                                                                                                      agttaTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAGTGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTg                                                <  1:1280502/150‑1 (MQ=255)
                                                                                                         tataTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGGACAAATTCCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGca                                             <  1:4325081/150‑1 (MQ=255)
                                                                                                         taTCTTCGTCTGTCATCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGca                                             <  2:1981149/150‑1 (MQ=255)
                                                                                                          aTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGcac                                            >  1:3582790/1‑150 (MQ=255)
                                                                                                          aTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGcac                                            <  2:3739662/150‑1 (MQ=255)
                                                                                                          aTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGcac                                            <  2:576412/150‑1 (MQ=255)
                                                                                                          aTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGAACTGATTTTTCTACTGACGGGCTGATGATACGTTATGTCACTGGCGGTAATAATCAGTGGcac                                            >  2:741224/1‑150 (MQ=255)
                                                                                                           tCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGcaca                                           <  1:905471/150‑1 (MQ=255)
                                                                                                              tCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTg                                        <  1:3420775/150‑1 (MQ=255)
                                                                                                                 tCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAg                                     >  1:1019702/1‑150 (MQ=255)
                                                                                                                  cTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGc                                    >  1:349827/1‑150 (MQ=255)
                                                                                                                       aGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTccc                               >  1:483761/1‑150 (MQ=255)
                                                                                                                       aGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTccc                               >  1:5146091/1‑150 (MQ=255)
                                                                                                                           gATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGca                           >  1:4130183/1‑150 (MQ=255)
                                                                                                                           gATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGca                           <  2:1637720/150‑1 (MQ=255)
                                                                                                                              tGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACAt                        <  2:1242753/150‑1 (MQ=255)
                                                                                                                              tGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACAt                        <  1:25120/150‑1 (MQ=255)
                                                                                                                               gAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATg                       <  1:1141239/150‑1 (MQ=255)
                                                                                                                                aaTGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGa                      >  1:3766668/1‑150 (MQ=255)
                                                                                                                                  tGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGAcc                    <  2:4408027/150‑1 (MQ=255)
                                                                                                                                  tGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGAcc                    >  2:2494128/1‑150 (MQ=255)
                                                                                                                                    gCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCAt                  <  2:864740/150‑1 (MQ=255)
                                                                                                                                      ggTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATaa                >  2:4139655/1‑150 (MQ=255)
                                                                                                                                       gTATATTTTCAGTACCAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAat               <  1:333465/150‑1 (MQ=255)
                                                                                                                                       gTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAat               >  1:2269039/1‑150 (MQ=255)
                                                                                                                                        tataTTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAatg              >  1:4664985/1‑150 (MQ=255)
                                                                                                                                              ttCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAAc        >  1:410723/1‑150 (MQ=255)
                                                                                                                                              ttCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCAATGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAAc        <  2:4443381/150‑1 (MQ=255)
                                                                                                                                              gtCAGTACATATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAAc        <  2:5012836/149‑1 (MQ=255)
                                                                                                                                               tCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACa       <  1:3742461/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATT  >  CP009273/4489394‑4489691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑