Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,521,271 C→A 5.8% A66A (GCG→GCT)  sgcX ← putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,521,2710CA 5.8% 1115.7 / 28.3 292A66A (GCG→GCT) sgcXputative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base C (136/138);  new base A (10/8);  total (146/146)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

GATACACCCCATGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCTGAAATC  >  CP009273/4521124‑4521418
                                                                                                                                                   |                                                                                                                                                   
gATACACCCCATGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATgcgc                                                                                                                                                   >  2:3825183/1‑150 (MQ=255)
 aTACACCCCATGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCa                                                                                                                                                  <  1:2454839/150‑1 (MQ=255)
  tACACCCCATGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCaa                                                                                                                                                 >  2:1405984/1‑150 (MQ=255)
         cATGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAatcat                                                                                                                                          >  2:1224368/1‑150 (MQ=255)
           tGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAAtcatca                                                                                                                                        <  2:5113281/150‑1 (MQ=255)
            gagtGGCACTGTGTCCCCGGTGAGGGTGTCGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCCTAAAACCGACTTCATCCATATGCGCTAAAATCATCAg                                                                                                                                       <  2:2706073/147‑1 (MQ=255)
            gACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAg                                                                                                                                       <  1:1100130/150‑1 (MQ=255)
            gACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAg                                                                                                                                       <  2:2458365/150‑1 (MQ=255)
             aCTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAATACCGACTTCATCCATATGCGCAAAAATCATCAGt                                                                                                                                      >  1:3536697/1‑150 (MQ=255)
             aCTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATCTGAGCAAAAATCATCAGt                                                                                                                                      >  2:2899217/1‑150 (MQ=255)
               tGGCCCTGTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCg                                                                                                                                    <  2:4228727/150‑1 (MQ=255)
                  cccTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAg                                                                                                                                 >  2:1293083/1‑150 (MQ=255)
                  cccTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAg                                                                                                                                 <  1:5192887/150‑1 (MQ=255)
                   ccTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAgc                                                                                                                                >  2:2600191/1‑150 (MQ=255)
                   ccTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCAAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCATTCGCAga                                                                                                                                >  2:1216149/1‑149 (MQ=255)
                        gTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATc                                                                                                                           >  1:726049/1‑150 (MQ=255)
                        gTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATc                                                                                                                           >  2:656531/1‑150 (MQ=255)
                         tCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGTCTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCg                                                                                                                          >  1:5117542/1‑150 (MQ=255)
                         tCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCg                                                                                                                          <  1:5069581/150‑1 (MQ=255)
                         tCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCg                                                                                                                          <  2:4164424/150‑1 (MQ=255)
                           cccGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCggg                                                                                                                        >  1:1170637/1‑150 (MQ=255)
                              ggTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGttt                                                                                                                     >  2:2276176/1‑150 (MQ=255)
                              ggTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGttt                                                                                                                     >  1:341328/1‑150 (MQ=255)
                              ggTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGttt                                                                                                                     >  1:3320087/1‑150 (MQ=255)
                                   gggTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCAc                                                                                                                >  2:3063081/1‑150 (MQ=255)
                                   gggTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCAc                                                                                                                <  1:2009834/150‑1 (MQ=255)
                                         cGATGGAAACAGCCATAGAGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGt                                                                                                          <  2:4980530/150‑1 (MQ=255)
                                              gAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACAATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAgcgc                                                                                                     >  1:1862004/1‑150 (MQ=255)
                                                aCCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAgcgcgc                                                                                                   >  1:4038715/1‑150 (MQ=255)
                                                   aGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGcgac                                                                                                <  2:1689894/150‑1 (MQ=255)
                                                     ccATAGTGACCTGCGCAGGACCGCCTACGCGTTCACAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATAAGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGcgacga                                                                                              <  2:389418/150‑1 (MQ=255)
                                                      cATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCgacgac                                                                                             <  2:4763018/150‑1 (MQ=255)
                                                          gTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTc                                                                                         >  2:5033562/1‑150 (MQ=255)
                                                          gTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTc                                                                                         >  2:2995024/1‑150 (MQ=255)
                                                            gACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTccc                                                                                       <  1:3238401/150‑1 (MQ=255)
                                                             aCCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCa                                                                                      <  1:1254692/150‑1 (MQ=255)
                                                               cTGCGCAGGACCGCCTACGCTTTAAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAg                                                                                    <  1:4311189/150‑1 (MQ=255)
                                                               cTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAg                                                                                    <  2:3400943/150‑1 (MQ=255)
                                                                tGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATAACTACCGTAGCGCGCGACGACATTCCCAAGc                                                                                   >  1:3091040/1‑150 (MQ=255)
                                                                  cgcAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCt                                                                                 <  1:3166347/150‑1 (MQ=255)
                                                                    cAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGt                                                                               >  1:2629198/1‑150 (MQ=255)
                                                                    cAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGt                                                                               <  2:1525716/150‑1 (MQ=255)
                                                                    cAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGt                                                                               <  2:2215863/150‑1 (MQ=255)
                                                                      ggACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGtct                                                                             <  1:2546542/150‑1 (MQ=255)
                                                                       gACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGtctc                                                                            <  1:2156740/150‑1 (MQ=255)
                                                                       gACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGtctc                                                                            <  1:2856800/150‑1 (MQ=255)
                                                                          cGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGcc                                                                         >  1:4821875/1‑150 (MQ=255)
                                                                            ccTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAg                                                                       >  1:868379/1‑150 (MQ=255)
                                                                             cTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGGGTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGa                                                                      >  1:3928908/1‑150 (MQ=255)
                                                                             cTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGa                                                                      >  2:3467241/1‑150 (MQ=255)
                                                                             cTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGa                                                                      >  1:1419039/1‑150 (MQ=255)
                                                                                cgcgTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACct                                                                   <  1:57007/150‑1 (MQ=255)
                                                                                           cGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGa                                                        >  2:4329718/1‑150 (MQ=255)
                                                                                            gTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGAc                                                       >  1:1307203/1‑150 (MQ=255)
                                                                                            gTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGAc                                                       >  2:1615770/1‑150 (MQ=255)
                                                                                            gTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGAc                                                       <  2:3891496/150‑1 (MQ=255)
                                                                                              aaaaaTCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGc                                                     >  2:3333550/1‑150 (MQ=255)
                                                                                                  aTCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTGGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGa                                                 >  1:4895194/1‑150 (MQ=255)
                                                                                                   tCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGaa                                                >  2:4063952/1‑150 (MQ=255)
                                                                                                   tCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGaa                                                <  2:2637157/150‑1 (MQ=255)
                                                                                                         cGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATACGca                                          >  2:1081651/1‑150 (MQ=255)
                                                                                                          ggCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGcac                                         >  1:991408/1‑150 (MQ=255)
                                                                                                           gCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGcaca                                        >  1:1037168/1‑150 (MQ=255)
                                                                                                           gCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGcaca                                        >  2:4456765/1‑150 (MQ=255)
                                                                                                            ctctATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCACATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAg                                       >  1:3712539/1‑150 (MQ=255)
                                                                                                              ctATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCa                                     >  1:5224143/1‑150 (MQ=255)
                                                                                                               tATTTTGCGCACCATAACACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCAt                                    <  1:5019593/150‑1 (MQ=255)
                                                                                                                    tGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGt                               >  1:1434470/1‑150 (MQ=255)
                                                                                                                     gcgcACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTc                              <  1:2994970/150‑1 (MQ=255)
                                                                                                                      cgcACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCa                             >  1:3632144/1‑150 (MQ=255)
                                                                                                                      cgcACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCa                             <  2:3272460/150‑1 (MQ=255)
                                                                                                                       gcACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAg                            <  2:3509782/150‑1 (MQ=255)
                                                                                                                        cACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGc                           <  2:3583861/150‑1 (MQ=255)
                                                                                                                        cACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGc                           <  2:1918729/150‑1 (MQ=255)
                                                                                                                         aCCATAACACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCg                          <  1:1634268/150‑1 (MQ=255)
                                                                                                                          ccATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGa                         <  1:2276176/150‑1 (MQ=255)
                                                                                                                           cATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGAc                        >  2:2767316/1‑150 (MQ=255)
                                                                                                                            aTAAAACCGCCTTCATCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGACTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGTCGTCAGCGACa                       <  1:3390903/150‑1 (MQ=255)
                                                                                                                            aTAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACa                       <  1:3887618/150‑1 (MQ=255)
                                                                                                                            aTAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACa                       >  1:1802226/1‑150 (MQ=255)
                                                                                                                            aTAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACa                       <  1:1271885/150‑1 (MQ=255)
                                                                                                                             tAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAg                      >  2:2986472/1‑150 (MQ=255)
                                                                                                                              aaaaCCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGc                     >  1:1691684/1‑150 (MQ=255)
                                                                                                                                  ccGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGttt                 <  2:196695/150‑1 (MQ=255)
                                                                                                                                   cGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGtttt                <  1:4623590/150‑1 (MQ=255)
                                                                                                                                    gACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTc               >  2:2092765/1‑150 (MQ=255)
                                                                                                                                    gACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTc               <  2:2568493/150‑1 (MQ=255)
                                                                                                                                     aCTTCATCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCg              <  2:731705/150‑1 (MQ=255)
                                                                                                                                        tCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCgtgt           >  2:3744767/1‑150 (MQ=255)
                                                                                                                                        tCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCgtgt           <  2:21593/150‑1 (MQ=255)
                                                                                                                                        tCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCgtgt           >  2:1127110/1‑150 (MQ=255)
                                                                                                                                        tCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCgtgt           <  2:4614033/150‑1 (MQ=255)
                                                                                                                                         cATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTc          >  1:1281284/1‑150 (MQ=255)
                                                                                                                                          aTCCATATGAGCAATAATCATAAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGAAGACATTCCAAAGCCTGACTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCCTGACGTCAGCGACAGCGATTTCGTGTcc         >  2:3958318/1‑150 (MQ=255)
                                                                                                                                          aTCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTcc         >  2:3113032/1‑150 (MQ=255)
                                                                                                                                           tCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCt        <  2:4658959/150‑1 (MQ=255)
                                                                                                                                           tCCATATGAGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCt        >  1:2694280/1‑150 (MQ=255)
                                                                                                                                              atatGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCTGaa     >  2:3047815/1‑150 (MQ=255)
                                                                                                                                              atatGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCTGaa     >  1:4756977/1‑150 (MQ=255)
                                                                                                                                                atGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCTGAAAt   <  2:827966/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATACACCCCATGACTGGCCCTTTGTCCCCGGTGAGGGTGACGATGGAACCAGCCATAGTGACCTGCGCAGGACCGCCTACGCGTTCAAAGCGTAAAAATCCAGACGGCTCTATTTTGCGCACCATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAGCGTTTTCGTGTCCTGAAATC  >  CP009273/4521124‑4521418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑