Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 358,609 G→T 5.2% I94I (ATC→ATA)  lacY ← lactose permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273358,6090GT 5.2% 1150.4 / 27.9 307I94I (ATC→ATA) lacYlactose permease
Reads supporting (aligned to +/- strand):  ref base G (140/151);  new base T (6/10);  total (146/161)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 307 total aligned reads displayed.

GCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCG  >  CP009273/358461‑358757
                                                                                                                                                    |                                                                                                                                                    
gcgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGa                                                                                                                                                     <  2:3680920/150‑1 (MQ=255)
   cgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATaa                                                                                                                                                  <  1:856758/150‑1 (MQ=255)
          aaaTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACACTCGATCCTACTCAGATGGTGGATTGGAACCGTGGCGCGAGGCTgaaaagtag                                                                                                                                           >  1:4101985/1‑146 (MQ=255)
            aTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGa                                                                                                                                         <  1:301617/150‑1 (MQ=255)
               cGAAATTACTGCGACGGCTGACTTTCTCAATAAATACCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACg                                                                                                                                      >  2:562118/1‑150 (MQ=255)
               cGAAATTACTGCGACGGCTGACTTTCTCAATAAATACCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACg                                                                                                                                      >  2:2767343/1‑150 (MQ=255)
                 aaaGTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGgc                                                                                                                                    <  2:4885066/150‑1 (MQ=255)
                      aCTGCGACGGCTGACTTTCTCAATAAAGGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCGAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCaaa                                                                                                                               <  2:1313641/150‑1 (MQ=255)
                          cGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATc                                                                                                                           >  1:2420313/1‑150 (MQ=255)
                           gACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAATATAAAAATAAAGAACGGCGCAAACATCa                                                                                                                          <  2:4324246/150‑1 (MQ=255)
                            aCGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCAc                                                                                                                         <  1:3517131/150‑1 (MQ=255)
                             cGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACt                                                                                                                        <  2:4164793/150‑1 (MQ=255)
                              ggCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTa                                                                                                                       <  1:2000373/150‑1 (MQ=255)
                              ggCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTa                                                                                                                       <  2:4436859/150‑1 (MQ=255)
                                 tGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACa                                                                                                                    <  1:5191271/150‑1 (MQ=255)
                                  gACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACAt                                                                                                                   >  1:1498135/1‑150 (MQ=255)
                                   aCTATCTCAATAAATGCCACTACTGCTGGCGCACCGGCGTAAAAACAAAAGACTAGATAAATACCACCAACAATCGATCCTACTCACATGATGTATTGTAACAGGGGCCAGAAGCTAAAAATAAACATCGGCGCTAACATCACTAACATg                                                                                                                  >  2:2760615/1‑150 (MQ=255)
                                    cTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCTAACATCACTAACATGc                                                                                                                 >  2:3108831/1‑150 (MQ=255)
                                    cTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGc                                                                                                                 >  2:4184614/1‑150 (MQ=255)
                                     tttCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGcc                                                                                                                <  1:3627773/150‑1 (MQ=255)
                                      ttCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCg                                                                                                               >  2:5241619/1‑150 (MQ=255)
                                        ctcAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGt                                                                                                             <  1:2665430/150‑1 (MQ=255)
                                             tAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGtaataa                                                                                                        <  1:4004430/150‑1 (MQ=255)
                                                aTGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATcc                                                                                                     >  1:3149481/1‑150 (MQ=255)
                                                   ccTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAATATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcaca                                                                                                  <  2:1067531/150‑1 (MQ=255)
                                                   ccTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcaca                                                                                                  >  1:170995/1‑150 (MQ=255)
                                                   ccTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcaca                                                                                                  >  1:3262042/1‑150 (MQ=255)
                                                   ccTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcaca                                                                                                  >  1:635192/1‑150 (MQ=255)
                                                   ccTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcaca                                                                                                  <  2:3060587/150‑1 (MQ=255)
                                                       tACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAACATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCAcagcag                                                                                              >  1:4597199/1‑150 (MQ=255)
                                                        aCTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAgg                                                                                             >  1:3464953/1‑150 (MQ=255)
                                                         ctgctgGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGt                                                                                            >  1:1933909/1‑150 (MQ=255)
                                                           gctgGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTAt                                                                                          <  2:5009010/150‑1 (MQ=255)
                                                             tgGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTAttt                                                                                        <  2:2396794/150‑1 (MQ=255)
                                                                cgcACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcg                                                                                     <  1:4159362/150‑1 (MQ=255)
                                                                  cacaGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGAACCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAAAAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCa                                                                                   >  2:3393730/1‑150 (MQ=255)
                                                                              aaCAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGt                                                                       >  1:1475880/1‑150 (MQ=255)
                                                                                cAACAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCa                                                                     <  2:4772110/150‑1 (MQ=255)
                                                                                cAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCa                                                                     <  2:2170329/150‑1 (MQ=255)
                                                                                cAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCa                                                                     >  1:3376777/1‑150 (MQ=255)
                                                                                 aaaaGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAg                                                                    >  1:1484382/1‑150 (MQ=255)
                                                                                  aaaGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGa                                                                   <  1:108804/150‑1 (MQ=255)
                                                                                  aaaGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGa                                                                   >  1:1550967/1‑150 (MQ=255)
                                                                                    aGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGaaa                                                                 <  1:922489/150‑1 (MQ=255)
                                                                                    aGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGaaa                                                                 >  2:4254614/1‑150 (MQ=255)
                                                                                          gATAAATACCACCCACCATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAATATCAAAATAAAGACCGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGAc                                                           <  2:1100866/150‑1 (MQ=255)
                                                                                          gATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGAc                                                           >  1:3661470/1‑150 (MQ=255)
                                                                                            tAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCa                                                         >  1:4680635/1‑150 (MQ=255)
                                                                                                  ccaccaACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATATAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGc                                                   <  1:4668437/150‑1 (MQ=255)
                                                                                                  ccaccaACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGc                                                   <  1:3796161/150‑1 (MQ=255)
                                                                                                  ccaccaACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGc                                                   <  2:3634734/150‑1 (MQ=255)
                                                                                                   caccaACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCg                                                  <  1:2767350/150‑1 (MQ=255)
                                                                                                   caccaACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCg                                                  <  2:1623704/150‑1 (MQ=255)
                                                                                                   caccaACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCg                                                  <  1:562118/150‑1 (MQ=255)
                                                                                                     ccaAAAATCGATCCTACTAAACTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGt                                                <  1:686639/150‑1 (MQ=255)
                                                                                                       aacaaTCGATCCTACTAAAATGTTGAATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTg                                              <  2:5126283/150‑1 (MQ=255)
                                                                                                         caaTCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGa                                            >  2:2523856/1‑150 (MQ=255)
                                                                                                          aaTCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACGAACATGCCGGTAATAATCCACAGCATGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGaa                                           <  2:2036442/150‑1 (MQ=255)
                                                                                                          aaTCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCAGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGaa                                           <  2:1939490/150‑1 (MQ=255)
                                                                                                           aTCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGaat                                          <  1:2150789/150‑1 (MQ=255)
                                                                                                            tCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGaata                                         >  2:4939851/1‑150 (MQ=255)
                                                                                                              gATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGaataat                                       <  1:2624378/150‑1 (MQ=255)
                                                                                                                tCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAg                                     >  1:206291/1‑150 (MQ=255)
                                                                                                                tCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAg                                     >  1:1738660/1‑150 (MQ=255)
                                                                                                                  ctactaAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCg                                   <  2:807438/150‑1 (MQ=255)
                                                                                                                    actaAACTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCgag                                 <  2:3839319/150‑1 (MQ=255)
                                                                                                                     ctaAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCgaga                                >  1:742063/1‑150 (MQ=255)
                                                                                                                       aaaaTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAAc                              >  1:3905943/1‑150 (MQ=255)
                                                                                                                        aaaTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACa                             >  1:407841/1‑150 (MQ=255)
                                                                                                                        aaaTGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACa                             >  1:4272675/1‑150 (MQ=255)
                                                                                                                         aaTGTTGTATTGTAACAGTGGCCCGAATATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACag                            >  2:626698/1‑150 (MQ=255)
                                                                                                                           tgttgtATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACagag                          <  2:1591194/150‑1 (MQ=255)
                                                                                                                           tgttgtATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACagag                          <  2:3464953/150‑1 (MQ=255)
                                                                                                                           tgttgtATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACagag                          <  2:847009/150‑1 (MQ=255)
                                                                                                                            gttgtATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAgaga                         <  2:4959985/150‑1 (MQ=255)
                                                                                                                            gttgtATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAgaga                         >  2:538872/1‑150 (MQ=255)
                                                                                                                            gttgtATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAgaga                         <  1:4924254/150‑1 (MQ=255)
                                                                                                                                ttttgtaACAGTTGACCGAAGATAAAAATAAAGAACGGCGCAAACATCACTATCATGCCGGTATTATTCCTCATCAGGTATTTGCGCAGCCCGAGTTTTTCAGAAAGCAGAGCAAAATGCGGTTGGAATAATAGCGAGAACAGAGAAATa                     <  1:3316401/148‑1 (MQ=255)
                                                                                                                                 attgtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAg                    >  2:1205254/1‑150 (MQ=255)
                                                                                                                                    gtaACAGTGGCCCGAATATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCgg                 >  1:3734202/1‑150 (MQ=255)
                                                                                                                                    gtaACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCgg                 >  1:1653450/1‑150 (MQ=255)
                                                                                                                                        cAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCACACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCaaa             >  1:4448811/1‑150 (MQ=255)
                                                                                                                                          gTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCaaaaa           >  2:4508566/1‑150 (MQ=255)
                                                                                                                                          gTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGAGGCaaaat           >  2:1900032/1‑149 (MQ=255)
                                                                                                                                           tGGCCCGAATATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaat          <  1:4862891/150‑1 (MQ=255)
                                                                                                                                            gtcccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaata         <  2:4066095/148‑1 (MQ=255)
                                                                                                                                            ggCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaata         <  2:4573679/150‑1 (MQ=255)
                                                                                                                                            ggCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaata         >  2:2039664/1‑150 (MQ=255)
                                                                                                                                             gCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaataa        >  1:5113066/1‑150 (MQ=255)
                                                                                                                                              cccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaataat       <  2:227116/150‑1 (MQ=255)
                                                                                                                                              cccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaataat       <  2:4420893/150‑1 (MQ=255)
                                                                                                                                              cccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAaataat       <  1:2248824/150‑1 (MQ=255)
                                                                                                                                               ccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAataata      >  1:1810880/1‑150 (MQ=255)
                                                                                                                                               ccGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAataata      <  1:1004306/150‑1 (MQ=255)
                                                                                                                                                cGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATAc     <  2:2605622/150‑1 (MQ=255)
                                                                                                                                                 gAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATAcc    <  2:2829421/150‑1 (MQ=255)
                                                                                                                                                 gAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATAcc    >  1:3056583/1‑150 (MQ=255)
                                                                                                                                                  aaGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATAccc   <  1:4495479/150‑1 (MQ=255)
                                                                                                                                                   aGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCg  <  2:705091/150‑1 (MQ=255)
                                                                                                                                                   aGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCg  >  2:3883406/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTTGTCAGAAAGCAGACCAAACAGCGGTTGGAATAATAGCGAGAACAGAGAAATAGCGGCAAAAATAATACCCG  >  CP009273/358461‑358757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑