BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,596,108	T→A	6.3%	intergenic (‑5/+24)	leuP ← / ← leuQ	tRNA‑Leu/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,596,108	0	T	A	6.3%	653.7 / 3.7	222	intergenic (‑5/+24)	leuP/leuQ	tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base T (123/77); new base A (18/3); total (142/80)
Fisher's exact test for biased strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGC  >  CP009273/4595959‑4596257

  cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                                                                                     >  1:2572924/1‑150 (MQ=255)

   tACCAATTCCGCCACCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                                                                                    <  1:2659436/150‑1 (MQ=255)

     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTCGCCAAGAGCCAg                                                                                                                                                  >  2:85827/1‑150 (MQ=255)

      ctaTTCAGCCAACTTATCTCATTCATCTTACTTTTTTTGATATAGACTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc                                                                                                                                                 <  2:1917284/148‑1 (MQ=18)

      cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc                                                                                                                                                 >  1:3726142/1‑150 (MQ=255)

       caTTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa                                                                                                                                                <  1:2698241/149‑1 (MQ=255)

          tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                                                                                                                             <  1:5173377/150‑1 (MQ=255)

           ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat                                                                                                                                            >  2:151730/1‑150 (MQ=255)

            cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGAGCGAGCACTATc                                                                                                                                           >  2:3297466/1‑150 (MQ=255)

              ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCGATATCaa                                                                                                                                         >  1:2044870/1‑150 (MQ=255)

                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                                                                                                                      >  1:800535/1‑150 (MQ=255)

                 acatctcacaGTCAACTTACTATTTTTGATTTCTCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                                                                                                                      <  2:2084707/144‑1 (MQ=18)

                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTg                                                                                                                                     >  1:796797/1‑150 (MQ=255)

                          attgaTCTTACTTTTTTTGATTTATCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                                                                                             <  2:4935302/146‑1 (MQ=21)

                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                                                                                             <  1:3540637/150‑1 (MQ=255)

                           gtgaTCTTACTTTTTTTGATCTCGCCTTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                                                                                                            <  2:2870204/147‑1 (MQ=255)

                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAACAGCGAGCACTATAAACGTGGTTTTTGGt                                                                                                                            >  2:538657/1‑150 (MQ=21)

                           gTCAACTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                                                                                                            <  2:231967/150‑1 (MQ=255)

                            tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCACTATCAACGTGGTTTTAGGTg                                                                                                                           >  1:4628003/1‑150 (MQ=255)

                              aTCTTACTATTTTTGATATCTCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                                                                                         <  2:1041879/150‑1 (MQ=255)

                               tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                        >  1:1723384/1‑150 (MQ=255)

                               tCTTACTTGTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                        <  2:5199525/150‑1 (MQ=255)

                                cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAg                                                                                                                       <  1:2521078/150‑1 (MQ=255)

                                  tACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTAACAACGGGGTGATTGGTGCGAggg                                                                                                                     >  2:4805256/1‑150 (MQ=21)

                                   aCTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                    >  1:1848341/1‑150 (MQ=255)

                                   aCTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTTCGAGAAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                    >  1:4313597/1‑150 (MQ=255)

                                     tttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                                  <  1:2913671/150‑1 (MQ=255)

                                       tttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACAGCCAGCCATAACAACGAGGTTATAGCTCCGAggcggcgg                                                                                                                >  2:5184376/1‑150 (MQ=17)

                                        ttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa                                                                                                               <  1:4497098/150‑1 (MQ=255)

                                        tattGATATCGCCTAGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa                                                                                                               <  1:4647248/148‑1 (MQ=255)

                                          ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGACCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACt                                                                                                             <  1:976434/150‑1 (MQ=255)

                                          ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACt                                                                                                             <  1:3598363/150‑1 (MQ=255)

                                            gATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtgg                                                                                                           >  1:1175659/1‑148 (MQ=255)

                                             atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATAAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa                                                                                                          >  2:4137558/1‑150 (MQ=255)

                                             atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa                                                                                                          <  1:954334/150‑1 (MQ=255)

                                             atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTATTGGTGCGAGGGGGGGGACTTGa                                                                                                          >  2:1528409/1‑150 (MQ=255)

                                             atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGAACTATCAACGTGTTTTTTGGTGCGAGGGGCGGGACTTGa                                                                                                          >  2:4047990/1‑150 (MQ=21)

                                             atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGAGCGAGAAATATCAACGTGGTGTTTGGTCCGAGGGGGGGGAATTGa                                                                                                          >  2:2299319/1‑150 (MQ=21)

                                                      cGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                                                                                 <  2:3794417/150‑1 (MQ=255)

                                                      cGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCCAGCACTATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAAACCCCACg                                                                                                 >  2:1553827/1‑150 (MQ=21)

                                                        tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc                                                                                               <  1:668307/150‑1 (MQ=255)

                                                         ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc                                                                                              >  1:5049502/1‑150 (MQ=255)

                                                         ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAAAACATACACCTGAATCTATCGCGTATACCCTGACCGCCACCTTCGCACATTGCGAGCAATATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc                                                                                              <  2:3605939/150‑1 (MQ=21)

                                                          tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGTACCCCCACGTCCg                                                                                             >  2:4980518/1‑150 (MQ=255)

                                                           ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGGGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAGTTGAAACCCCACGTCCga                                                                                            >  2:5013158/1‑149 (MQ=255)

                                                             tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACACCCACGTcctaaa                                                                                          >  2:2489374/1‑146 (MQ=255)

                                                                gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGa                                                                                       >  2:634936/1‑150 (MQ=255)

                                                                 aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCCCAGTTCGAGCAATATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                      <  1:3982280/150‑1 (MQ=255)

                                                                     gggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                  >  1:1479559/1‑150 (MQ=255)

                                                                         gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                              >  1:5023177/1‑150 (MQ=255)

                                                                         gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGCCACCTTCGCCCAGAGCGAGCACAAACAACGAGTTTTTTGGTGCGAGGGGCGGGACTTGAACCCCCACGTCCGTCAGGACACTAacac                                                                              >  1:4127000/1‑150 (MQ=21)

                                                                          aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                             <  2:2454030/150‑1 (MQ=255)

                                                                           ctttaccccccACGTCCGTAAGAACACTAACACATGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCACAGAGCGAGCAATATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                                            <  2:2240030/144‑1 (MQ=25)

                                                                               aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                        >  2:4592131/1‑150 (MQ=255)

                                                                               aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTGTTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCGAAAGGACACTAACACCTGAAg                                                                        >  2:4276412/1‑150 (MQ=255)

                                                                                aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                                                       >  1:497862/1‑150 (MQ=255)

                                                                                                gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                       >  1:4229610/1‑150 (MQ=255)

                                                                                                  acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                     >  1:416618/1‑150 (MQ=255)

                                                                                                   ccagaaCACCTGAATCTAGCGCCTCGACCAATTCCGCCACCTACGCAAAGACCTAGAAATTTCAACTTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                    <  2:2830132/146‑1 (MQ=17)

                                                                                                    acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                   >  1:1506685/1‑150 (MQ=255)

                                                                                                        acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTTCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCACACGTCCGTAAGGACACTAACACCTGAAGCTAGAGCGTCTACCAATTCCGCCAc                                               >  1:102933/1‑150 (MQ=255)

                                                                                                        acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                               >  2:4007208/1‑150 (MQ=255)

                                                                                                        acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                               >  1:3925577/1‑150 (MQ=255)

                                                                                                         cacCTGAAGCTAGCGAGTCTACCAATTACGCCAACTTCGCCCAGTGCGAGCAATATCAAAGTGGTTTAAGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAATGACACTAACACCTGAAGCTAGAGCGTATACAAATTCCGcaaac                                              >  2:3071969/1‑146 (MQ=21)

                                                                                                          acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                             >  1:3033978/1‑150 (MQ=255)

                                                                                                            cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                           >  1:4684148/1‑150 (MQ=255)

                                                                                                            cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                           >  1:1210922/1‑150 (MQ=255)

                                                                                                             tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                          >  2:2357162/1‑150 (MQ=255)

                                                                                                              gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTGTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCGACCTTCGc                                         >  2:1896104/1‑150 (MQ=255)

                                                                                                                   tAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGAGCGAGGGGGGGGACTGGAACCCCCACGTCCGTAAGGACACTAACACCTGCAGCTAGCGCGTCTACCAATTCCGCCACCATCGCAGAcc                                    >  1:3198628/1‑150 (MQ=21)

                                                                                                                       gcgTCTACCACGTCCTCCTCCTTCGACACGTGCGAGCTATATCAACGTGTTATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCa                                <  1:4405737/150‑1 (MQ=17)

                                                                                                                         gTCTACCTTTTCCGACACAGTCGCACCTTTCTAGCAATTTAAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt                              <  1:4613826/150‑1 (MQ=14)

                                                                                                                         gTCTACCAAGTCCTCCACATTCGCACAGTTCGATCAATTACAACGTGGATTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt                              <  2:800535/150‑1 (MQ=17)

                                                                                                                          tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt                             >  2:1379123/1‑150 (MQ=255)

                                                                                                                           cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                            >  2:1564579/1‑150 (MQ=255)

                                                                                                                            tACCAATTCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt                           <  1:1417718/150‑1 (MQ=255)

                                                                                                                             aCCATTTCCCCCACCTTCTCCCTGTTCCAGCTATTTATACGTGTTTTTTGGTTAGAGGGGTGTGAATTGAAGCTCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                          <  1:2391307/150‑1 (MQ=17)

                                                                                                                              ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTa                         >  1:47451/1‑150 (MQ=255)

                                                                                                                              ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTa                         >  2:1630160/1‑150 (MQ=255)

                                                                                                                               cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                        >  2:4728668/1‑150 (MQ=255)

                                                                                                                               cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                        >  2:1198632/1‑150 (MQ=255)

                                                                                                                               cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACGTTAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCAAGCCCGGATACCAAATACCCCACCTTCGCATAACATCCATACTTaa                        >  1:3811379/1‑150 (MQ=21)

                                                                                                                                aaTTCCGCCACCTTCGCACAGTGCGAGCAATATCAACGTGGTTTTTGGAGCTAGGGGGGGGACTTGAACCCCCACGTCAGTAAGCACACTAACACCTGAAGCTATAGCGTCTACAAATTCCGCCACCTTCTCATACAATCAATTCTTaaa                       >  2:3308626/1‑150 (MQ=21)

                                                                                                                                  ttCCGCCACCTTCGACCAGTTCTTTCTTTTTCAAAGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa                     <  1:227444/150‑1 (MQ=17)

                                                                                                                                   tCCGCCACCTTGTCCCAGTTCGTTAATTTACAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAg                    <  2:1495248/150‑1 (MQ=17)

                                                                                                                                    ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                   >  1:1638758/1‑150 (MQ=255)

                                                                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                  >  2:5152714/1‑150 (MQ=255)

                                                                                                                                         aCCTTCGCCCATTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGc              <  1:3397773/150‑1 (MQ=255)

                                                                                                                                          ccTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt             <  2:1723384/150‑1 (MQ=255)

                                                                                                                                           cTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa            >  2:134677/1‑150 (MQ=255)

                                                                                                                                            ttCGCCCAGTTCTTTCTATCTCAACGTGGTTTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc           <  1:2762677/150‑1 (MQ=18)

                                                                                                                                             tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc          >  2:861793/1‑150 (MQ=255)

                                                                                                                                             tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc          >  1:4789622/1‑150 (MQ=255)

                                                                                                                                             tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc          >  1:2189938/1‑150 (MQ=255)

                                                                                                                                               gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc        >  2:2987403/1‑150 (MQ=255)

                                                                                                                                                cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg       >  1:4433962/1‑150 (MQ=255)

                                                                                                                                                   aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg    >  2:2640530/1‑150 (MQ=255)

                                                                                                                                                    gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg   >  1:2481994/1‑150 (MQ=255)

                                                                                                                                                     tGCGAGCAATATCCACGTGGATTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc  <  1:2873912/150‑1 (MQ=255)

                                                                                                                                                     tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc  >  1:2376891/1‑150 (MQ=255)

GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGC  >  CP009273/4595959‑4596257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`