Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 434,663 A→C 68.2% I324S (ATC→AGC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273434,6630AC68.2% 586.2 / 315.0 342I324S (ATC→AGC) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base A (50/58);  new base C (134/99);  total (184/158)
Fisher's exact test for biased strand distribution p-value = 6.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

GCGTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGCG  >  CP009273/434516‑434810
                                                                                                                                                   |                                                                                                                                                   
gcgTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtt                                                                                                                                                   <  1:1535596/150‑1 (MQ=255)
 cgTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCttt                                                                                                                                                  <  2:3326007/150‑1 (MQ=255)
  gtgtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtttt                                                                                                                                                 >  1:3363505/1‑150 (MQ=255)
  gtgtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCtttt                                                                                                                                                 <  1:771536/150‑1 (MQ=255)
   tgtTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCttttt                                                                                                                                                <  2:5173673/150‑1 (MQ=255)
        cTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATa                                                                                                                                           >  1:3342776/1‑150 (MQ=255)
         tCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAg                                                                                                                                          <  1:1053860/150‑1 (MQ=255)
          cGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGACCCTTCACGCATCGCCGGAGTAATCGCCCTCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGc                                                                                                                                         >  2:2227557/1‑150 (MQ=255)
          cGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGc                                                                                                                                         >  1:3194359/1‑150 (MQ=255)
          cGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGc                                                                                                                                         >  1:3378188/1‑150 (MQ=255)
          cGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGc                                                                                                                                         >  2:4826742/1‑150 (MQ=255)
           ggCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCt                                                                                                                                        <  1:976827/150‑1 (MQ=255)
             catttGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCg                                                                                                                                      <  2:1252538/147‑1 (MQ=255)
              aaTTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCgg                                                                                                                                     >  2:3426623/1‑150 (MQ=255)
              aaTTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCgg                                                                                                                                     >  1:2877294/1‑150 (MQ=255)
                   ccACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAAc                                                                                                                                >  1:4238318/1‑150 (MQ=255)
                    cACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAcc                                                                                                                               <  2:4418764/150‑1 (MQ=255)
                     aCGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccg                                                                                                                              >  2:3801051/1‑150 (MQ=255)
                       gTCGAAGTAGCGCTCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAAccgcc                                                                                                                            <  2:3535252/150‑1 (MQ=255)
                       gTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAAccgcc                                                                                                                            >  1:1972407/1‑150 (MQ=255)
                          gAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACAATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCt                                                                                                                         <  1:4913615/150‑1 (MQ=255)
                           acggaGCGATCCGTGAATTTACGTGAAAACTCGACCAGGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTa                                                                                                                        <  2:2813042/146‑1 (MQ=255)
                             gTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACt                                                                                                                      <  1:1159324/150‑1 (MQ=255)
                             gTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACt                                                                                                                      <  2:4717798/150‑1 (MQ=255)
                                 cGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTc                                                                                                                  >  2:1175807/1‑150 (MQ=255)
                                 cGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTc                                                                                                                  <  1:3221760/150‑1 (MQ=255)
                                        ggAATTTACGTGAACACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTACTCGCCATCAGCTTGTTGTCGTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaac                                                                                                           <  1:1278911/150‑1 (MQ=255)
                                         gAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaaca                                                                                                          >  1:3006961/1‑150 (MQ=255)
                                         gAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAaaca                                                                                                          <  1:2701451/150‑1 (MQ=255)
                                            tttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAAcc                                                                                                       <  2:779913/150‑1 (MQ=255)
                                             ttACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCg                                                                                                      <  2:4109174/150‑1 (MQ=255)
                                               aCGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCt                                                                                                    <  1:1884755/150‑1 (MQ=255)
                                                 gTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTgg                                                                                                  >  1:3490751/1‑150 (MQ=255)
                                                  tGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCACAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACGTTTCGGCACACACCCGCGGGa                                                                                                 >  2:4047418/1‑150 (MQ=255)
                                                     aaaCTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAggg                                                                                              >  2:2901919/1‑150 (MQ=255)
                                                       aCTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGAt                                                                                            <  1:5024001/150‑1 (MQ=255)
                                                           gACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCaaa                                                                                        >  2:1261418/1‑150 (MQ=255)
                                                               aTGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTa                                                                                    <  1:532037/150‑1 (MQ=255)
                                                                tGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAg                                                                                   <  1:5185557/150‑1 (MQ=255)
                                                                 gCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAgg                                                                                  >  2:3700663/1‑150 (MQ=255)
                                                                       aCCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGc                                                                            <  1:2826000/150‑1 (MQ=255)
                                                                        ccTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCg                                                                           >  1:2740673/1‑150 (MQ=255)
                                                                        ccTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCg                                                                           >  1:1798635/1‑150 (MQ=255)
                                                                              cGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGaaa                                                                     >  1:5125054/1‑150 (MQ=255)
                                                                              cGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGATGGAGGGATCAAATTTAGGCACGGCGTGGaaa                                                                     >  2:5226222/1‑150 (MQ=255)
                                                                                cATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGt                                                                   >  2:5083880/1‑150 (MQ=255)
                                                                                    gCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATc                                                               >  1:4403903/1‑150 (MQ=255)
                                                                                       ggAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGAGCAAATTTAGGCACGGCGTGGAACGTGATCggg                                                            >  1:2491313/1‑150 (MQ=255)
                                                                                       ggAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCggg                                                            >  2:565253/1‑150 (MQ=255)
                                                                                          gTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                         >  2:1110658/1‑150 (MQ=255)
                                                                                          gTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCt                                                         >  1:3719876/1‑150 (MQ=255)
                                                                                           tAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCtt                                                        >  1:2219324/1‑150 (MQ=255)
                                                                                            aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttt                                                       >  1:2590084/1‑150 (MQ=255)
                                                                                            aaTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttt                                                       >  2:157253/1‑150 (MQ=255)
                                                                                             aTCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCtttt                                                      <  2:459588/150‑1 (MQ=255)
                                                                                              tCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttttt                                                     <  2:4411823/150‑1 (MQ=255)
                                                                                              tCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttttt                                                     <  2:4504847/150‑1 (MQ=255)
                                                                                              tCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttttt                                                     <  1:4115462/150‑1 (MQ=255)
                                                                                              tCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCttttt                                                     <  2:3179505/150‑1 (MQ=255)
                                                                                                gCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCt                                                   >  1:3835957/1‑150 (MQ=255)
                                                                                                gCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCt                                                   <  1:3125584/150‑1 (MQ=255)
                                                                                                gCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCt                                                   <  1:4030921/150‑1 (MQ=255)
                                                                                                 ccATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTg                                                  <  2:1453941/150‑1 (MQ=255)
                                                                                                   aTCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGcc                                                <  1:464936/150‑1 (MQ=255)
                                                                                                       gCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGtt                                            <  1:4899930/150‑1 (MQ=255)
                                                                                                        cTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTc                                           >  2:2142307/1‑150 (MQ=255)
                                                                                                        cTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTc                                           >  2:3069377/1‑150 (MQ=255)
                                                                                                        cTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTATTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGACAGTGATCGGGTCTGTTTCTGCCGGtga                                           >  2:2808587/1‑148 (MQ=255)
                                                                                                        cTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTc                                           <  2:4280585/150‑1 (MQ=255)
                                                                                                         ttgttgTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCa                                          <  2:2470989/150‑1 (MQ=255)
                                                                                                         tgtgcgtCTTTCGCTGCCGGTTCGCACAACCAGTCGCCAAAGCTTTGTGAAGAGCTCGGCAAACCGCCGCTACGTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCa                                          <  2:2696799/145‑1 (MQ=255)
                                                                                                           gttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATa                                        <  2:1379967/150‑1 (MQ=255)
                                                                                                           gttgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATa                                        <  2:126687/150‑1 (MQ=255)
                                                                                                             tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAAc                                      >  1:4512319/1‑150 (MQ=255)
                                                                                                             tgtCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAAc                                      <  1:2107833/150‑1 (MQ=255)
                                                                                                               tCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCa                                    <  2:4617124/150‑1 (MQ=255)
                                                                                                                 tttCGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAACCCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACg                                  <  2:3669480/150‑1 (MQ=255)
                                                                                                                    cGCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGAcc                               <  2:1586076/150‑1 (MQ=255)
                                                                                                                    cGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGAcc                               >  1:4424689/1‑150 (MQ=255)
                                                                                                                     gCTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCt                              <  2:1051309/150‑1 (MQ=255)
                                                                                                                      cTGCCGTTTCGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCtt                             >  2:249270/1‑150 (MQ=255)
                                                                                                                          cttttCGCACAACCCGTCGCCAAAGATATATGAATAGCTCGGAAAACCACCGCTACTTTTCGGCAAACAACAGATGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTAACTGCCGCTTCATAACCACGACCtttttt                         >  2:202324/3‑150 (MQ=255)
                                                                                                                            tttCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTgg                       >  2:869398/1‑150 (MQ=255)
                                                                                                                             ttCGCACAACCAGTCGCCAAAGCTTTTTGAAGAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAACTTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATACCCCCGACCTTTTTTGGt                      <  2:2065426/150‑1 (MQ=255)
                                                                                                                               cGCACAACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCa                    <  2:3006961/150‑1 (MQ=255)
                                                                                                                                  acaACCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGa                 <  2:3948877/150‑1 (MQ=255)
                                                                                                                                    aaCCAGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGata               >  1:3571372/1‑150 (MQ=255)
                                                                                                                                        aGTCGCCAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCa           >  1:4274691/1‑150 (MQ=255)
                                                                                                                                        aGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCa           <  1:4712572/150‑1 (MQ=255)
                                                                                                                                         gTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAg          >  1:65371/1‑150 (MQ=255)
                                                                                                                                         gTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAg          <  1:2302958/150‑1 (MQ=255)
                                                                                                                                             ccAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAAc      <  2:1272376/150‑1 (MQ=255)
                                                                                                                                              cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACt     <  2:1825189/150‑1 (MQ=255)
                                                                                                                                              cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACt     >  1:4185844/1‑150 (MQ=255)
                                                                                                                                              cAAAGCTTTTTGAATAGCTCGGCAAACCGCCGCTAC‑TTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGACAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTg    >  2:3084462/1‑150 (MQ=255)
                                                                                                                                              cAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACt     >  1:2145172/1‑150 (MQ=255)
                                                                                                                                               aaaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTg    >  1:859321/1‑150 (MQ=255)
                                                                                                                                               aaaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTg    >  1:1465355/1‑150 (MQ=255)
                                                                                                                                               aaaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTg    >  1:3052851/1‑150 (MQ=255)
                                                                                                                                                aaGCTTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGc   <  1:2535388/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGTGTTGCTCGGCAATTGCCACGTCGAAGTAGCGATCCGGGAATTTACGTGAAAACTCGACCATGCCGGAACCTTCACGCATCGCCGGAGTAATCGCCATCAGCTTGTTGTCTTTCGCTGCCGTTTCGCACAACCAGTCGCCAAAGATTTTTGAATAGCTCGGCAAACCGCCGCTACTTTTCGGCAAACAACCGCTGGAGGGATCAAATTTAGGCACGGCGTGGAAAGTGATCGGGTCTTTTTCTGCCGGTTCATAACCACGACCTTTTTTGGTCATGATATGCAGGAACTGCG  >  CP009273/434516‑434810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑