Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 495,583 G→T 5.5% M1M (ATG→ATT) † gsk → inosine/guanosine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273495,5830GT 5.5% 1241.1 / 32.6 332M1M (ATG→ATT) †gskinosine/guanosine kinase
Reads supporting (aligned to +/- strand):  ref base G (162/151);  new base T (11/8);  total (173/159)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

AAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGA  >  CP009273/495434‑495732
                                                                                                                                                     |                                                                                                                                                     
aaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATg                                                                                                                                                       <  1:3000892/150‑1 (MQ=255)
aaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATg                                                                                                                                                       >  2:1600703/1‑150 (MQ=255)
  aCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTCAAAACAACCATGaa                                                                                                                                                     >  2:550541/1‑150 (MQ=255)
    tCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAAt                                                                                                                                                   <  2:3497249/150‑1 (MQ=255)
      cTTTCAAATTACTTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAAttt                                                                                                                                                 <  2:2916652/150‑1 (MQ=255)
      cTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAAttt                                                                                                                                                 <  2:3725011/150‑1 (MQ=255)
         tCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTccc                                                                                                                                              >  1:2799783/1‑150 (MQ=255)
          cAACTTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCg                                                                                                                                             <  2:3795791/150‑1 (MQ=255)
          cAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCg                                                                                                                                             >  2:3554258/1‑150 (MQ=255)
           aaaTTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCgg                                                                                                                                            >  1:404703/1‑150 (MQ=255)
           aaaTTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCgg                                                                                                                                            >  1:2967565/1‑150 (MQ=255)
            aaTTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGt                                                                                                                                           >  1:94400/1‑150 (MQ=255)
            aaTTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCCGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGt                                                                                                                                           <  1:1655265/150‑1 (MQ=255)
               tACGTCATTGTAAGGAACGACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTaaa                                                                                                                                        <  1:2467073/150‑1 (MQ=255)
                aCGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAAc                                                                                                                                       >  2:1813794/1‑150 (MQ=255)
                 cGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGAAAACg                                                                                                                                      >  2:1322354/1‑150 (MQ=255)
                  gTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGt                                                                                                                                     <  1:265880/150‑1 (MQ=255)
                  gTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGt                                                                                                                                     >  2:222857/1‑150 (MQ=255)
                    cATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTaa                                                                                                                                   >  1:4811042/1‑150 (MQ=255)
                      ttGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAAt                                                                                                                                 <  2:722864/150‑1 (MQ=255)
                        gTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAACCGTAAATcc                                                                                                                               <  2:4917686/150‑1 (MQ=255)
                        gTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATcc                                                                                                                               <  1:3368814/150‑1 (MQ=255)
                          aaGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGACTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCaa                                                                                                                             >  2:1495234/1‑150 (MQ=255)
                          aaGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCaa                                                                                                                             <  2:2719998/150‑1 (MQ=255)
                            ggAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGACTCGCGCGCTCCCGTCAGATTGAGTTAACATTCGCCGCTCAGTTAACCACACGTAAAAAAAACCATTAAATTACCCGGTAAACGTAAATCCaaaa                                                                                                                           >  1:41556/1‑149 (MQ=255)
                              aaCCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACAt                                                                                                                         <  2:1065321/150‑1 (MQ=255)
                               aCCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACAtt                                                                                                                        <  1:1054706/150‑1 (MQ=255)
                               aCCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACAtt                                                                                                                        <  1:4374686/150‑1 (MQ=255)
                               aCCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACAtt                                                                                                                        <  1:2454800/150‑1 (MQ=255)
                                  aCTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACt                                                                                                                     >  1:210605/1‑150 (MQ=255)
                                     gCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTcc                                                                                                                  <  2:1381533/150‑1 (MQ=255)
                                     gCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTcc                                                                                                                  <  2:1978290/150‑1 (MQ=255)
                                       cATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTcccc                                                                                                                >  2:4201499/1‑150 (MQ=255)
                                        aTGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCg                                                                                                               <  2:3941270/150‑1 (MQ=255)
                                         tGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGt                                                                                                              >  1:829838/1‑150 (MQ=255)
                                         tGAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGt                                                                                                              >  1:3446421/1‑150 (MQ=255)
                                         tGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGt                                                                                                              <  1:2640888/150‑1 (MQ=255)
                                          gAAAATGCGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGAGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTa                                                                                                             <  2:797785/150‑1 (MQ=255)
                                          gAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTa                                                                                                             <  2:1467346/150‑1 (MQ=255)
                                                 cGATCCCGCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgcac                                                                                                      <  2:1291942/150‑1 (MQ=255)
                                                  gATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgcacg                                                                                                     <  2:4537875/150‑1 (MQ=255)
                                                   aTCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAacgcacgc                                                                                                    >  1:3722344/1‑150 (MQ=255)
                                                     cccGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGa                                                                                                  >  2:628858/1‑150 (MQ=255)
                                                      ccGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGAt                                                                                                 <  1:1617014/150‑1 (MQ=255)
                                                      cagCCTTCTTATATAGAAACTGGCTGCGTCTAGCGCGCTCCAGTAAGATTTTGTTAACATTCTCCGCTCATTTAACCACCCGTAAAAAAAACCATGAAATTTCCCGGTAAACTTAAATCCAAACATAACATCACCGTAAACCAACGCGAt                                                                                                 >  2:2392704/3‑150 (MQ=255)
                                                       cGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATTAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATc                                                                                                <  1:749108/150‑1 (MQ=255)
                                                       cGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATc                                                                                                <  2:4440038/150‑1 (MQ=255)
                                                        gCCTGCTGATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATcc                                                                                               <  2:2124233/150‑1 (MQ=255)
                                                        gCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATcc                                                                                               <  2:2963123/150‑1 (MQ=255)
                                                         ccTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCg                                                                                              >  1:1198444/1‑150 (MQ=255)
                                                           tgctgATATTGTAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTGCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCgct                                                                                            <  2:3644252/150‑1 (MQ=255)
                                                           tgctgATATTGAAACTGGCTGCGTCTCGCGAGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCgct                                                                                            <  2:1524888/150‑1 (MQ=255)
                                                            gctgATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCgctg                                                                                           <  1:3672258/150‑1 (MQ=255)
                                                                atatTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTc                                                                                       >  2:2101371/1‑150 (MQ=255)
                                                                 tatTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCa                                                                                      <  1:2876234/150‑1 (MQ=255)
                                                                      taaCTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAAt                                                                                 >  1:1551407/2‑150 (MQ=255)
                                                                       aaCTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGTTCCGCTGCTGCAGCACtt                                                                                >  1:3630574/1‑150 (MQ=255)
                                                                        aCTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTc                                                                               <  1:230332/150‑1 (MQ=255)
                                                                          tGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTcca                                                                             >  1:4002456/1‑150 (MQ=255)
                                                                            gCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCCTGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTccagc                                                                           <  1:3637236/150‑1 (MQ=255)
                                                                            gCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTccagc                                                                           >  2:1118219/1‑150 (MQ=255)
                                                                               gCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTccagccag                                                                        >  1:892819/1‑150 (MQ=255)
                                                                                 gTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGaa                                                                      >  1:403905/1‑150 (MQ=255)
                                                                                   ctcGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGaaaa                                                                    >  2:4703793/1‑150 (MQ=255)
                                                                                        gcgcTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGaaa                                                               <  1:375228/150‑1 (MQ=255)
                                                                                           cTCCCGTCAGATTTTGTTAACATTCGCCGATCAGTTAACCACCCTTAAAAACAACCTTGAAATTTCCCTGTTAACGTTAATCCAATAATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCa                                                            <  1:4409356/150‑1 (MQ=255)
                                                                                           cTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCa                                                            <  1:1800812/150‑1 (MQ=255)
                                                                                           cTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCa                                                            >  2:2170483/1‑150 (MQ=255)
                                                                                             cccGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAgc                                                          >  1:1955289/1‑150 (MQ=255)
                                                                                                 tCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTg                                                      <  1:3567948/150‑1 (MQ=255)
                                                                                                     agtgtgTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTg                                                  <  2:1699904/148‑1 (MQ=255)
                                                                                                     aTTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTg                                                  <  2:5204834/150‑1 (MQ=255)
                                                                                                        gtgtTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCTGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGt                                               >  1:3904003/1‑150 (MQ=255)
                                                                                                        gtgtTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGt                                               >  2:1778173/1‑150 (MQ=255)
                                                                                                         tgtTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTa                                              <  2:4314754/150‑1 (MQ=255)
                                                                                                          gtTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAg                                             >  2:948810/1‑150 (MQ=255)
                                                                                                           ttAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGt                                            >  1:869709/1‑150 (MQ=255)
                                                                                                            tAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTg                                           >  2:3437409/1‑150 (MQ=255)
                                                                                                              aCATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATTAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTggg                                         >  1:4617010/1‑150 (MQ=255)
                                                                                                                aTTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTa                                       <  1:329357/150‑1 (MQ=255)
                                                                                                                  tCGCCGCTCAGTTAACCACCCGTAAAAACAACCATTAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATc                                     <  2:4060694/150‑1 (MQ=255)
                                                                                                                     ccgcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGAt                                  <  1:652135/150‑1 (MQ=255)
                                                                                                                      cgcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTCGTGGGTATCGATc                                 >  1:4234363/1‑150 (MQ=255)
                                                                                                                      cgcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACGTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATc                                 <  2:4789430/150‑1 (MQ=255)
                                                                                                                       gcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCa                                <  2:3123892/150‑1 (MQ=255)
                                                                                                                         tCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCaaa                              >  2:3607836/1‑150 (MQ=255)
                                                                                                                            gTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGc                           >  1:469836/1‑150 (MQ=255)
                                                                                                                            gTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGc                           >  1:548775/1‑150 (MQ=255)
                                                                                                                             ttAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCt                          >  2:4740293/1‑150 (MQ=255)
                                                                                                                             ttAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCt                          >  2:5095713/1‑150 (MQ=255)
                                                                                                                               aaccaaccGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTTTCGATCAAACGCTgg                        <  1:4182174/150‑1 (MQ=255)
                                                                                                                               aaCCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTgg                        <  2:2799783/150‑1 (MQ=255)
                                                                                                                                 ccaccCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCAAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTc                      >  1:1281916/1‑150 (MQ=255)
                                                                                                                                  caccCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCg                     >  1:716863/1‑150 (MQ=255)
                                                                                                                                        tAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTg               >  1:5032492/1‑150 (MQ=255)
                                                                                                                                          aaaaCAACCATTAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCGGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGaa             >  2:823437/1‑150 (MQ=255)
                                                                                                                                          aaaaCAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGaa             <  2:643217/150‑1 (MQ=255)
                                                                                                                                            aacaacCATGAAATTTCCCGGGAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGc           <  2:850205/150‑1 (MQ=255)
                                                                                                                                               aacCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGaa        >  2:2543156/1‑150 (MQ=255)
                                                                                                                                                  cATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGt     >  2:2348359/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGA  >  CP009273/495434‑495732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑