Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 505,336 C→T 8.9% E500E (GAG→GAA)  copA ← copper transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273505,3360CT 8.9% 970.5 / 59.4 291E500E (GAG→GAA) copAcopper transporter
Reads supporting (aligned to +/- strand):  ref base C (132/132);  new base T (9/17);  total (142/149)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 295 total aligned reads displayed.

ATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCGCACT  >  CP009273/505188‑505485
                                                                                                                                                    |                                                                                                                                                     
aTGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACt                                                                                                                                                      <  2:1024616/150‑1 (MQ=255)
 tGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTc                                                                                                                                                     <  2:1698104/150‑1 (MQ=255)
    cTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgc                                                                                                                                                  >  2:3119474/1‑150 (MQ=255)
    cTGCGCTACATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgc                                                                                                                                                  >  2:485815/1‑150 (MQ=255)
       cgcTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgc                                                                                                                                               >  1:1212708/1‑150 (MQ=255)
        gcTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc                                                                                                                                              >  1:2814481/1‑150 (MQ=255)
         cTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgccc                                                                                                                                             <  1:3356458/150‑1 (MQ=255)
           tcatcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGccgcccgc                                                                                                                                           >  2:3601589/1‑150 (MQ=255)
            catcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCcgcccgcc                                                                                                                                          >  1:3719834/1‑150 (MQ=255)
              tcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGcccgcccg                                                                                                                                        <  1:1654723/150‑1 (MQ=255)
               caACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGa                                                                                                                                       >  2:3766812/1‑150 (MQ=255)
                aaCATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGAc                                                                                                                                      >  2:883497/1‑150 (MQ=255)
                aaCATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGAc                                                                                                                                      <  2:714709/150‑1 (MQ=255)
                 aCATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACg                                                                                                                                     <  2:4527513/150‑1 (MQ=255)
                   aTCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGcc                                                                                                                                   >  1:3342173/1‑150 (MQ=255)
                   aTCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGcc                                                                                                                                   <  1:3398159/150‑1 (MQ=255)
                   aTCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGcc                                                                                                                                   >  1:1698850/1‑150 (MQ=255)
                     cAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCgg                                                                                                                                 >  1:534461/1‑150 (MQ=255)
                     cAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCgg                                                                                                                                 >  2:4087703/1‑150 (MQ=255)
                        cAAATGTATTCACAGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCACGGTTTTATCGAACACTACAGTGTCGAGTGAACTGGCTCGTATCAGCGCGTCATAGTCCCGCACCAGCACTCCAAACTCAGACGCCCGCCCCACGCCGCaaa                                                                                                                              >  2:3927806/1‑150 (MQ=255)
                         aaaTGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaat                                                                                                                             >  1:238024/1‑150 (MQ=255)
                          aaTGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaata                                                                                                                            <  1:903266/150‑1 (MQ=255)
                          aaTGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaata                                                                                                                            <  1:2649057/150‑1 (MQ=255)
                          aaTGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaata                                                                                                                            >  2:2186914/1‑150 (MQ=255)
                            tGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaataat                                                                                                                          <  1:589547/150‑1 (MQ=255)
                                  cACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                    <  2:3714282/150‑1 (MQ=255)
                                  cACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                    >  1:5216779/1‑150 (MQ=255)
                                   aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATc                                                                                                                   >  1:1605113/1‑150 (MQ=255)
                                    cTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCg                                                                                                                  >  2:1824345/1‑150 (MQ=255)
                                       cGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCg                                                                                                               <  2:2160153/150‑1 (MQ=255)
                                       cGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCg                                                                                                               <  2:2266874/150‑1 (MQ=255)
                                         aCAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTc                                                                                                             <  2:2398953/150‑1 (MQ=255)
                                           aaCCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGc                                                                                                           >  2:4355134/1‑150 (MQ=255)
                                            aCCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGcc                                                                                                          <  1:4131268/150‑1 (MQ=255)
                                             ccTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCa                                                                                                         >  2:3266120/1‑150 (MQ=255)
                                              cTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCATCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCTCCAg                                                                                                        <  2:351303/150‑1 (MQ=255)
                                              cTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGAGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAg                                                                                                        <  2:2244248/150‑1 (MQ=255)
                                                  ggCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGcccc                                                                                                    <  1:2395032/150‑1 (MQ=255)
                                                  ggCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGTCATCGGCGTCGCCAGcccc                                                                                                    >  2:3216/1‑150 (MQ=255)
                                                  ggCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGcccc                                                                                                    <  2:732969/150‑1 (MQ=255)
                                                  ggCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGcccc                                                                                                    >  1:2937076/1‑150 (MQ=255)
                                                   gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCTAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCa                                                                                                   >  2:1447022/1‑150 (MQ=255)
                                                   gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCa                                                                                                   <  2:4160520/150‑1 (MQ=255)
                                                   gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCa                                                                                                   >  1:3914712/1‑150 (MQ=255)
                                                    cTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAg                                                                                                  <  2:2123969/150‑1 (MQ=255)
                                                    cTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAg                                                                                                  >  2:2009629/1‑150 (MQ=255)
                                                      tCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAgcg                                                                                                >  1:678491/1‑150 (MQ=255)
                                                         cTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGcac                                                                                             <  1:727889/150‑1 (MQ=255)
                                                              gtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGa                                                                                        <  1:1147285/150‑1 (MQ=255)
                                                                 agCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAg                                                                                     <  1:2584637/150‑1 (MQ=255)
                                                                   cGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGc                                                                                   <  1:4064934/150‑1 (MQ=255)
                                                                       ccGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaata                                                                               <  1:3949333/150‑1 (MQ=255)
                                                                          gtattATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGACCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCACCGCACACGGACAGGCAATAATc                                                                            >  2:3771054/4‑150 (MQ=255)
                                                                          gTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATc                                                                            >  1:1416224/1‑150 (MQ=255)
                                                                           ttttATCGAACACTCCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCa                                                                           <  2:2802693/150‑1 (MQ=255)
                                                                           ttttATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCa                                                                           <  1:1800549/150‑1 (MQ=255)
                                                                            tttATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAg                                                                          <  2:303721/150‑1 (MQ=255)
                                                                              tATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTa                                                                        <  2:4553361/150‑1 (MQ=255)
                                                                              tATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTa                                                                        <  1:463778/150‑1 (MQ=255)
                                                                               aTCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAc                                                                       <  1:3646800/150‑1 (MQ=255)
                                                                               aTCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAc                                                                       <  1:5171584/150‑1 (MQ=255)
                                                                                tCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAcc                                                                      <  2:1363156/150‑1 (MQ=255)
                                                                                  gAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgt                                                                    >  1:1937280/1‑150 (MQ=255)
                                                                                    acacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtgg                                                                  >  1:3058606/1‑150 (MQ=255)
                                                                                    acacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtgg                                                                  <  2:290236/150‑1 (MQ=255)
                                                                                    acacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtgg                                                                  <  2:2872015/150‑1 (MQ=255)
                                                                                     cacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCCATAATCAGTACCgtggt                                                                 >  2:3110831/1‑150 (MQ=255)
                                                                                     cacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtggt                                                                 <  2:2522433/150‑1 (MQ=255)
                                                                                      acTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtggtg                                                                >  1:4240646/1‑150 (MQ=255)
                                                                                      acTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtggtg                                                                <  2:1579997/150‑1 (MQ=255)
                                                                                         aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCa                                                             <  1:4347353/150‑1 (MQ=255)
                                                                                         aCAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCa                                                             <  2:754133/150‑1 (MQ=255)
                                                                                                 gagaGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCATTACCGTGGTGGCAATCACCAg                                                     >  2:1455919/1‑150 (MQ=255)
                                                                                                  aGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAgg                                                    >  1:4979799/1‑150 (MQ=255)
                                                                                                      tACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGtat                                                <  2:2320768/150‑1 (MQ=255)
                                                                                                      tACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGgat                                                >  1:2207945/1‑150 (MQ=255)
                                                                                                       aCTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGtata                                               <  1:2080767/150‑1 (MQ=255)
                                                                                                        cTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAg                                              >  2:1829060/1‑150 (MQ=255)
                                                                                                         tGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                             >  1:4230104/1‑150 (MQ=255)
                                                                                                         tGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGGATAGa                                             >  1:3589690/1‑150 (MQ=255)
                                                                                                            cgcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACaa                                          <  1:2980811/150‑1 (MQ=255)
                                                                                                             gcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAAt                                         >  2:2999351/1‑150 (MQ=255)
                                                                                                                 tGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGc                                     <  2:4174345/150‑1 (MQ=255)
                                                                                                                  gCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAACTAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCg                                    <  2:2432404/150‑1 (MQ=255)
                                                                                                                  gCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCg                                    <  1:669504/150‑1 (MQ=255)
                                                                                                                    aGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGt                                  >  2:3172837/1‑150 (MQ=255)
                                                                                                                    aGCGAGTCAGCGTCCCGCACCAGCTCGCCAAACTCAGCCGCCCGCCCGACGCCGTAAATAATCGTCATCGTGGTCGCCAGCCCCTGAGCAGTCGGACAGGCAATAATGAGTACCGTTGTGGCAATCACCAGGGTATAGACAATCTGCgtt                                  <  2:80927/150‑3 (MQ=255)
                                                                                                                     gcgcGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTg                                 <  2:1197011/150‑1 (MQ=255)
                                                                                                                      cgcgTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGc                                <  2:5052079/150‑1 (MQ=255)
                                                                                                                        cgTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCg                              <  1:3065680/150‑1 (MQ=255)
                                                                                                                              cGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCaa                        >  1:417331/1‑150 (MQ=255)
                                                                                                                                tCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAg                      <  1:2447311/150‑1 (MQ=255)
                                                                                                                                  ccGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGaa                    <  2:3417773/150‑1 (MQ=255)
                                                                                                                                         aGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTGCCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAg             <  2:5185926/150‑1 (MQ=255)
                                                                                                                                          gCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGa            <  1:475189/150‑1 (MQ=255)
                                                                                                                                           cACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGAt           <  2:4454068/150‑1 (MQ=255)
                                                                                                                                            aCGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGAtt          <  2:4978048/150‑1 (MQ=255)
                                                                                                                                             cGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCCATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCCAAGAAATACCAGATTg         >  1:1016528/1‑150 (MQ=255)
                                                                                                                                               ccAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGcc       <  1:3447401/150‑1 (MQ=255)
                                                                                                                                                  aaCTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCGCa    >  2:2452949/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCGCACT  >  CP009273/505188‑505485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑