Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 832,574 G→T 10.5% T256T (ACG→ACT)  ybiC → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273832,5740GT10.5% 1139.0 / 79.6 324T256T (ACG→ACT) ybiCputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (151/139);  new base T (21/13);  total (172/152)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 336 total aligned reads displayed.

ATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTCGCCGCATGATGATG  >  CP009273/832425‑832722
                                                                                                                                                     |                                                                                                                                                    
  tGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCa                                                                                                                                                    >  2:4582208/1‑150 (MQ=255)
     cGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCa                                                                                                                                                 <  1:5586108/150‑1 (MQ=255)
     cGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCa                                                                                                                                                 >  2:2594893/1‑150 (MQ=255)
      gTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAg                                                                                                                                                <  1:1127793/150‑1 (MQ=255)
      gTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAg                                                                                                                                                >  2:561782/1‑150 (MQ=255)
          aCGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGaaa                                                                                                                                            <  2:4826274/150‑1 (MQ=255)
              cGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGtt                                                                                                                                        <  1:4298945/150‑1 (MQ=255)
              cGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCATTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGtt                                                                                                                                        <  1:4460289/150‑1 (MQ=255)
               gTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa                                                                                                                                       >  1:757777/1‑150 (MQ=255)
               gTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa                                                                                                                                       <  1:2027799/150‑1 (MQ=255)
               gTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa                                                                                                                                       >  2:3726146/1‑150 (MQ=255)
                tGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTAc                                                                                                                                      <  2:779075/150‑1 (MQ=255)
                tGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTAc                                                                                                                                      <  1:3311337/150‑1 (MQ=255)
                 gCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACa                                                                                                                                     <  1:4036113/150‑1 (MQ=255)
                   cgacgaCCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACTCATCAGGAAACGTTACaaa                                                                                                                                   <  1:5271525/150‑1 (MQ=255)
                   cgacgaCCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACaaa                                                                                                                                   >  1:4914160/1‑150 (MQ=255)
                    gacgacCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAAc                                                                                                                                  >  1:709148/1‑150 (MQ=255)
                        acCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGt                                                                                                                              >  1:3806380/1‑150 (MQ=255)
                           aaTCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTccc                                                                                                                           >  1:2923552/1‑150 (MQ=255)
                               cggcggTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATg                                                                                                                       <  2:5072027/150‑1 (MQ=255)
                                      aaTGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCt                                                                                                                <  1:1719482/150‑1 (MQ=255)
                                       aTGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCtt                                                                                                               <  1:816020/150‑1 (MQ=255)
                                       aTGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCtt                                                                                                               <  1:2131698/150‑1 (MQ=255)
                                             gAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                                                                                                         >  1:1046745/1‑150 (MQ=255)
                                              aGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCa                                                                                                        <  2:4248366/150‑1 (MQ=255)
                                                tCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATg                                                                                                      >  2:4481574/1‑150 (MQ=255)
                                                       tGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTa                                                                                               <  1:1092809/150‑1 (MQ=255)
                                                        gggTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTat                                                                                              <  1:170726/150‑1 (MQ=255)
                                                         ggTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatc                                                                                             <  2:1448161/150‑1 (MQ=255)
                                                         ggTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatc                                                                                             >  1:4329848/1‑150 (MQ=255)
                                                         ggTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatc                                                                                             >  1:4516999/1‑150 (MQ=255)
                                                           ttCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcat                                                                                           <  2:3453237/150‑1 (MQ=255)
                                                            tCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatc                                                                                          >  1:4653892/1‑150 (MQ=255)
                                                             cGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatca                                                                                         <  1:956095/150‑1 (MQ=255)
                                                             cGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatca                                                                                         >  1:1532302/1‑150 (MQ=255)
                                                             cGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatca                                                                                         >  2:4751192/1‑150 (MQ=255)
                                                              gCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatcat                                                                                        <  2:1366896/150‑1 (MQ=255)
                                                               cTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatcatc                                                                                       >  2:2532138/1‑150 (MQ=255)
                                                                tGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTAtcatcatca                                                                                      <  1:97145/150‑1 (MQ=255)
                                                                tGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTAtcatcatca                                                                                      <  1:5538945/150‑1 (MQ=255)
                                                                   tGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAAcc                                                                                   >  1:4752233/1‑150 (MQ=255)
                                                                    gACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAAccc                                                                                  >  2:864383/1‑150 (MQ=255)
                                                                        tttGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGaa                                                                              <  1:1315375/150‑1 (MQ=255)
                                                                         ttGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAAc                                                                             >  2:2742362/1‑150 (MQ=255)
                                                                          tGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAAct                                                                            <  1:1766027/150‑1 (MQ=255)
                                                                           gCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAActc                                                                           >  1:5313708/1‑150 (MQ=255)
                                                                            ccGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAActct                                                                          <  2:3360011/150‑1 (MQ=255)
                                                                            ccGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAActct                                                                          <  2:504026/150‑1 (MQ=255)
                                                                              gAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTc                                                                        <  1:3090153/150‑1 (MQ=255)
                                                                              gAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTc                                                                        <  1:259779/150‑1 (MQ=255)
                                                                              gAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTc                                                                        <  2:2360393/150‑1 (MQ=255)
                                                                               aaCATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCg                                                                       <  2:4028003/150‑1 (MQ=255)
                                                                                     aaGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGgcgcgc                                                                 >  2:1521185/1‑150 (MQ=255)
                                                                                     aaGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGgcgcgc                                                                 >  1:672902/1‑150 (MQ=255)
                                                                                      aGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGcc                                                                <  2:576673/150‑1 (MQ=255)
                                                                                       ggCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCg                                                               <  2:210489/150‑1 (MQ=255)
                                                                                        gCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACCGCATGACCACTCTCATCATCAACCCGGAACTCTTCGGCGCGCCgg                                                              >  2:4109429/1‑150 (MQ=255)
                                                                                         cTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGa                                                             >  1:136738/1‑150 (MQ=255)
                                                                                              cccTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTa                                                        <  1:4967220/150‑1 (MQ=255)
                                                                                                cTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAAc                                                      <  1:5157596/150‑1 (MQ=255)
                                                                                                 ttGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAAcg                                                     <  1:3417403/150‑1 (MQ=255)
                                                                                                  tGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAAcgc                                                    >  1:2422970/1‑150 (MQ=255)
                                                                                                     aGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCa                                                 >  1:3655865/1‑150 (MQ=255)
                                                                                                       cGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGa                                               >  2:552075/1‑150 (MQ=255)
                                                                                                       cGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGa                                               >  1:3904728/1‑150 (MQ=255)
                                                                                                       cGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGa                                               >  2:1653610/1‑150 (MQ=255)
                                                                                                        gATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGAc                                              >  2:2429827/1‑150 (MQ=255)
                                                                                                        gATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGAc                                              >  2:3935136/1‑150 (MQ=255)
                                                                                                        gATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGAc                                              <  1:4281328/150‑1 (MQ=255)
                                                                                                         aTGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACTCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGAcc                                             >  2:1712087/1‑150 (MQ=255)
                                                                                                          tgtgtgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCg                                            <  2:5435201/150‑1 (MQ=255)
                                                                                                           gtgtgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGa                                           >  1:3925690/1‑150 (MQ=255)
                                                                                                            tgtgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACTCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGaa                                          <  2:1265208/150‑1 (MQ=255)
                                                                                                             gtgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTCTCATCATCAACCCGGAACTCTTCGGCGCGCCGGAGTGTAACGCGCAGACCGAAg                                         >  2:2957964/1‑150 (MQ=255)
                                                                                                             gtgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAg                                         >  1:1850733/1‑150 (MQ=255)
                                                                                                              tgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGc                                        <  1:4187957/150‑1 (MQ=255)
                                                                                                              tgAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGc                                        <  1:695609/150‑1 (MQ=255)
                                                                                                                aaaTTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGccc                                      <  1:577952/150‑2 (MQ=255)
                                                                                                                 aaTTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCtt                                     >  1:2790152/1‑150 (MQ=255)
                                                                                                                 aaTTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCtt                                     >  2:5376339/1‑150 (MQ=255)
                                                                                                                  aTTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCttt                                    >  2:4889179/1‑150 (MQ=255)
                                                                                                                  aTTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCttt                                    >  2:3103553/1‑150 (MQ=255)
                                                                                                                   ttCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTg                                   <  2:2805841/150‑1 (MQ=255)
                                                                                                                   ttCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTg                                   <  2:4519088/150‑1 (MQ=255)
                                                                                                                      ttGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCg                                <  1:3726146/150‑1 (MQ=255)
                                                                                                                       tGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGACACGTTACAAACCAGTCCCGATGCCATGCTTCACTGCCTGACCACTCGCATCATCAACCCGGAACTCTTCGGCGCGCCGGAGTGTACCGCGCAGACCGAAGCCGTTGCCgc                               >  2:3507106/1‑149 (MQ=255)
                                                                                                                       tGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGa                               >  2:2866890/1‑150 (MQ=255)
                                                                                                                        ggCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAg                              >  2:743916/1‑150 (MQ=255)
                                                                                                                           ggggCGCTTTCCGGCGGTAAAACGACTCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTgg                           >  1:5205930/1‑150 (MQ=255)
                                                                                                                                 cTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGaaa                     >  1:2525276/1‑150 (MQ=255)
                                                                                                                                  tttCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAg                    >  2:436044/1‑150 (MQ=255)
                                                                                                                                      cggcggTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTc                <  1:2594789/150‑1 (MQ=255)
                                                                                                                                      cggcggTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTc                <  1:3675349/150‑1 (MQ=255)
                                                                                                                                      cggcggTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTc                >  1:2230368/1‑150 (MQ=255)
                                                                                                                                      cggcggTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCAGTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGGGGGTGAAAGCCTc                >  2:1868263/1‑150 (MQ=255)
                                                                                                                                       ggaggTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTcg               <  2:3168338/147‑1 (MQ=255)
                                                                                                                                               aaCGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTCGCCGCatga       >  1:4024108/1‑150 (MQ=255)
                                                                                                                                                 cgacgCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTCGCCGCatgatg     <  2:1989696/150‑1 (MQ=255)
                                                                                                                                                   acgCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTCGCCGCatgatgat   <  2:2249856/150‑1 (MQ=255)
                                                                                                                                                   acgCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTCGCCGCatgatgat   <  1:4002165/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCGAAGCCTTTGCCGAGTGGGTGAAAGCCTCGCCGCATGATGATG  >  CP009273/832425‑832722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑