Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,193,313 C→T 5.4% M127I (ATG→ATA)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,193,3130CT 5.4% 1117.9 / 33.0 315M127I (ATG→ATA) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (156/138);  new base T (9/8);  total (167/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 320 total aligned reads displayed.

ATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAA  >  CP009273/1193164‑1193462
                                                                                                                                                     |                                                                                                                                                     
    gtttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTa                                                                                                                                                   >  1:3449629/1‑150 (MQ=255)
    gtttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTa                                                                                                                                                   >  1:3808977/1‑150 (MQ=255)
     tttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                                                                                                                                                  <  2:4996209/150‑1 (MQ=255)
     tttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                                                                                                                                                  <  1:4836541/150‑1 (MQ=255)
      ttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACa                                                                                                                                                 <  2:260157/150‑1 (MQ=255)
      ttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACa                                                                                                                                                 <  1:3260710/150‑1 (MQ=255)
       tgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACAt                                                                                                                                                <  1:415549/150‑1 (MQ=255)
        gCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACAtt                                                                                                                                               >  1:5202825/1‑150 (MQ=255)
         cAAAAAAAGATTAATAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtg                                                                                                                                              <  2:2954771/150‑1 (MQ=255)
            aaaaaGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATGGTGa                                                                                                                                           <  2:436034/150‑1 (MQ=255)
              aaaGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGc                                                                                                                                         >  1:2214176/1‑150 (MQ=255)
              aaaGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAAATTGTGAGc                                                                                                                                         >  2:1729600/1‑150 (MQ=255)
                  attattAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCa                                                                                                                                     >  2:5659655/1‑150 (MQ=255)
                    tattaGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATc                                                                                                                                   <  2:5646837/150‑1 (MQ=255)
                     attaGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCt                                                                                                                                  <  1:2260394/150‑1 (MQ=255)
                          gaaggaaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttatt                                                                                                                             >  2:449945/1‑150 (MQ=255)
                           aaggaaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttattt                                                                                                                            <  2:2240246/150‑1 (MQ=255)
                             ggaaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTaa                                                                                                                          <  1:115247/150‑1 (MQ=255)
                              gaaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAAt                                                                                                                         >  2:80082/1‑150 (MQ=255)
                                aaTTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt                                                                                                                       >  2:4777871/1‑150 (MQ=255)
                                   tAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTTATTACATTGTGAGCTTCATCTTTATTTAAttttat                                                                                                                    >  2:1812205/1‑148 (MQ=255)
                                   tAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCt                                                                                                                    >  1:5593143/1‑150 (MQ=255)
                                    aaTGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                                                                                   >  2:1443254/1‑150 (MQ=255)
                                      tGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGtt                                                                                                                 >  1:1026704/1‑150 (MQ=255)
                                         aaTTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGAc                                                                                                              >  1:1661170/1‑150 (MQ=255)
                                          aTTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACt                                                                                                             >  2:2854690/1‑150 (MQ=255)
                                           ttATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTc                                                                                                            >  1:669618/1‑150 (MQ=255)
                                                 aaaaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGctc                                                                                                      >  1:4047474/1‑150 (MQ=255)
                                                  aaaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTAATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGTCTCCAgaaat                                                                                                     >  1:5456509/1‑146 (MQ=255)
                                                   aaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCtctc                                                                                                    >  1:393393/1‑150 (MQ=255)
                                                    aTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTcc                                                                                                   <  2:37445/150‑1 (MQ=255)
                                                      tCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTTATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                                                                                 <  2:3408938/150‑1 (MQ=255)
                                                       cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTg                                                                                                >  1:2661457/1‑150 (MQ=255)
                                                         aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGAt                                                                                              <  2:4119336/150‑1 (MQ=255)
                                                          aaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATa                                                                                             <  2:2169107/150‑1 (MQ=255)
                                                           aaaTATGCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATTATGTTTGTCTTCAATTTTTCTTACTTGAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa                                                                                            <  2:5284266/150‑1 (MQ=255)
                                                           aaaTATCCAAAAATTGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa                                                                                            <  2:4582467/150‑1 (MQ=255)
                                                                 ccAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGttt                                                                                      <  2:5438319/150‑1 (MQ=255)
                                                                  cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtttt                                                                                     <  1:4571180/150‑1 (MQ=255)
                                                                   aaaaaTATTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta                                                                                    <  1:1222939/150‑1 (MQ=255)
                                                                   aaaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta                                                                                    >  1:3282506/1‑150 (MQ=255)
                                                                    aaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtat                                                                                   <  2:5211462/150‑1 (MQ=255)
                                                                     aaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                                                                                  >  2:2151822/1‑150 (MQ=255)
                                                                     aaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                                                                                  <  1:1529804/150‑1 (MQ=255)
                                                                       aTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAAt                                                                                <  1:4651133/150‑1 (MQ=255)
                                                                        ttctataCTTTATACCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAAtt                                                                               <  2:3032928/147‑1 (MQ=255)
                                                                        tAGTATACTGTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAAtt                                                                               <  2:27239/150‑1 (MQ=255)
                                                                         aGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                                                                              <  1:804975/150‑1 (MQ=255)
                                                                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTTATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAg                                                                             >  1:2043854/1‑150 (MQ=255)
                                                                             taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg                                                                          <  1:5099400/150‑1 (MQ=255)
                                                                                   aTTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATc                                                                    <  1:83792/150‑1 (MQ=255)
                                                                                     tCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc                                                                  <  2:137626/150‑1 (MQ=255)
                                                                                     tCCAGAAGAGTTCAATATAATGTTTGTATTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTAATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc                                                                  <  2:5369495/150‑1 (MQ=255)
                                                                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTTTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGATTATCCCa                                                                 >  2:5259261/1‑150 (MQ=255)
                                                                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCa                                                                 >  2:2441232/1‑150 (MQ=255)
                                                                                        agaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa                                                               >  1:526761/1‑150 (MQ=255)
                                                                                         gaagaGTTCAATATAATTTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg                                                              <  2:3291096/150‑1 (MQ=255)
                                                                                          aagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTTTGAGCTTCATCTTTATTTAATATTATTTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGATTATCACAAAGa                                                             >  2:1554682/1‑150 (MQ=255)
                                                                                             agTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGatat                                                          >  1:1864124/1‑150 (MQ=255)
                                                                                                tCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGc                                                       >  2:417299/1‑150 (MQ=255)
                                                                                                   atatAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCAcc                                                    >  2:2516415/1‑150 (MQ=255)
                                                                                                     ataATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCg                                                  >  2:4638933/1‑150 (MQ=255)
                                                                                                      taATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGa                                                 >  2:1086959/1‑150 (MQ=255)
                                                                                                       aaTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGaa                                                >  2:3576592/1‑150 (MQ=255)
                                                                                                          gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAagta                                             >  1:99147/1‑150 (MQ=255)
                                                                                                               tCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTg                                        <  2:1113101/150‑1 (MQ=255)
                                                                                                                 ttCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGc                                      >  2:1332564/1‑150 (MQ=255)
                                                                                                                 ttCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGc                                      >  1:4977399/1‑150 (MQ=255)
                                                                                                                   cAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTg                                    >  1:2402946/1‑150 (MQ=255)
                                                                                                                    aaTTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGc                                   <  1:5307534/150‑1 (MQ=255)
                                                                                                                    aaTTTTTCTTACGTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGc                                   <  2:511347/150‑1 (MQ=255)
                                                                                                                     aTTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCa                                  <  2:4083982/150‑1 (MQ=255)
                                                                                                                      tttttCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCAt                                 <  2:1303531/150‑1 (MQ=255)
                                                                                                                      tttttCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCAt                                 <  2:846042/150‑1 (MQ=255)
                                                                                                                        tttCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTg                               >  1:2809411/1‑150 (MQ=255)
                                                                                                                          tCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGtt                             >  1:516096/1‑150 (MQ=255)
                                                                                                                              aCTTCAGGGTACTATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGt                         <  1:4363720/150‑1 (MQ=255)
                                                                                                                              aCTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGt                         <  1:5572468/150‑1 (MQ=255)
                                                                                                                                ttCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAg                       >  1:5015501/1‑150 (MQ=255)
                                                                                                                                 tCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAgg                      >  1:4415437/1‑150 (MQ=255)
                                                                                                                                 tCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAgg                      >  2:4231489/1‑150 (MQ=255)
                                                                                                                                   actatAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTc                    <  1:1408871/146‑1 (MQ=255)
                                                                                                                                   aGGGTAATATAGATTGCTTATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTc                    >  1:751057/1‑150 (MQ=255)
                                                                                                                                   aGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTc                    <  2:1212475/150‑1 (MQ=255)
                                                                                                                                   aGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTc                    >  1:1762162/1‑150 (MQ=255)
                                                                                                                                   aGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTc                    <  1:2084334/150‑1 (MQ=255)
                                                                                                                                    gggTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCt                   >  1:392434/1‑150 (MQ=255)
                                                                                                                                     ggTAATATAGATTGCTTATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTa                  <  1:5468263/150‑1 (MQ=255)
                                                                                                                                       tAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAAc                >  2:4884678/1‑150 (MQ=255)
                                                                                                                                              gATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGc         >  1:3337027/1‑150 (MQ=255)
                                                                                                                                               aTTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCt        <  1:2444318/150‑1 (MQ=255)
                                                                                                                                                ttGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTa       >  2:3837683/1‑150 (MQ=255)
                                                                                                                                                 tGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTaa      <  1:2854690/150‑1 (MQ=255)
                                                                                                                                                 tGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTaa      >  1:3613871/1‑150 (MQ=255)
                                                                                                                                                  gCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAAt     <  2:4670064/150‑1 (MQ=255)
                                                                                                                                                   cTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAAtt    >  1:3228807/1‑150 (MQ=255)
                                                                                                                                                   cTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAAtt    >  2:349433/1‑150 (MQ=255)
                                                                                                                                                    tCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTa   >  1:3556316/1‑150 (MQ=255)
                                                                                                                                                    tCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTa   >  1:3011106/1‑150 (MQ=255)
                                                                                                                                                     cATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTaa  <  2:1036589/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAA  >  CP009273/1193164‑1193462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑