Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,229,460 G→T 9.9% F238L (TTC→TTA)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,229,4600GT 9.9% 1329.0 / 82.8 371F238L (TTC→TTA) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (183/151);  new base T (15/22);  total (198/173)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 396 total aligned reads displayed.

GGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTT  >  CP009273/1229312‑1229608
                                                                                                                                                    |                                                                                                                                                    
 gcTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGgaagaa                                                                                                                                                    >  2:3230689/1‑150 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAAt                                                                                                                                                  <  2:2538221/150‑1 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAAt                                                                                                                                                  <  1:508795/150‑1 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAAt                                                                                                                                                  >  1:130512/1‑150 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAAt                                                                                                                                                  >  2:3131314/1‑150 (MQ=255)
       aTCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGcc                                                                                                                                              >  2:4863162/1‑150 (MQ=255)
          gTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaaa                                                                                                                                           >  1:137816/1‑150 (MQ=255)
            cAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAAt                                                                                                                                         <  2:4821529/150‑1 (MQ=255)
             aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATg                                                                                                                                        >  1:1605515/1‑150 (MQ=255)
             aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATg                                                                                                                                        >  1:5317285/1‑150 (MQ=255)
              aaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                       >  2:4636939/1‑150 (MQ=255)
               aCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATgcc                                                                                                                                      >  2:559017/1‑150 (MQ=255)
                 tGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccag                                                                                                                                    >  2:3642314/1‑150 (MQ=255)
                 tGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccag                                                                                                                                    <  2:1571561/150‑1 (MQ=255)
                    tGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccagcca                                                                                                                                 >  1:1058710/1‑150 (MQ=255)
                    tGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccagcca                                                                                                                                 >  2:386904/1‑150 (MQ=255)
                      cAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCcagccagc                                                                                                                               >  1:1023931/1‑150 (MQ=255)
                           cTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                          <  1:666533/150‑1 (MQ=255)
                            ttCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCttt                                                                                                                         <  2:1262247/150‑1 (MQ=255)
                              cgcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAg                                                                                                                       >  2:4017208/1‑150 (MQ=255)
                              cgcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAg                                                                                                                       <  2:436027/150‑1 (MQ=255)
                               gcgGACTTTCTCCGGCAGCGCTTCACCGTAGCCAAACCAACGCAGCGTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCACAACCGGAACGGTCACCGGCGACATGCTCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGc                                                                                                                      <  2:1595203/150‑1 (MQ=255)
                                 ggACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCga                                                                                                                    >  1:96989/1‑150 (MQ=255)
                                 ggACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCga                                                                                                                    <  1:1443629/150‑1 (MQ=255)
                                 ggACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCga                                                                                                                    <  1:4055279/150‑1 (MQ=255)
                                  gACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgat                                                                                                                   >  1:1141888/1‑150 (MQ=255)
                                   aCTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatg                                                                                                                  <  2:3040773/150‑1 (MQ=255)
                                    cTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCCACGCAGCTTCTCTCCCAGCAGGCAGGTTAACAGGCCACAAATCCGAACCGGAACGGGCACCGGCGACATGCGCAGGAAGAAATCGCCAAAAGGCCAGCCAGCCGCTTTAGCgatga                                                                                                                 <  1:511965/150‑1 (MQ=255)
                                     tttCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatgat                                                                                                                <  1:1191640/150‑1 (MQ=255)
                                      ttCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATc                                                                                                               <  2:4681445/150‑1 (MQ=255)
                                        ctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAg                                                                                                             <  1:4655626/150‑1 (MQ=255)
                                           cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGACGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                          >  1:5376420/1‑150 (MQ=255)
                                           cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                          >  1:5559623/1‑150 (MQ=255)
                                            ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGACGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTc                                                                                                         >  2:3840484/1‑150 (MQ=255)
                                            ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTCGCGATGATCAGGTTc                                                                                                         >  2:5147076/1‑150 (MQ=255)
                                              cAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtg                                                                                                       >  2:5536541/1‑150 (MQ=255)
                                               aGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgt                                                                                                      <  2:4989979/150‑1 (MQ=255)
                                                gCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgtg                                                                                                     >  1:4123442/1‑150 (MQ=255)
                                                 cGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTgg                                                                                                    >  2:3581037/1‑150 (MQ=255)
                                                  gTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCCGCCGCTTTAGCGATGATCAGGTTCTGTGGt                                                                                                   >  2:3340162/1‑150 (MQ=255)
                                                   tttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGtt                                                                                                  <  2:1494407/150‑1 (MQ=255)
                                                   tttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGtt                                                                                                  >  1:5583602/1‑150 (MQ=255)
                                                     tCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCg                                                                                                <  2:4145000/150‑1 (MQ=255)
                                                       aCCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGcc                                                                                              <  2:3734353/150‑1 (MQ=255)
                                                           tAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACCGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGACGAAATCGCCCAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacc                                                                                          >  2:677101/1‑150 (MQ=255)
                                                            aGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacca                                                                                         >  2:1080205/1‑150 (MQ=255)
                                                            aGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacca                                                                                         >  2:3617545/1‑150 (MQ=255)
                                                              ccAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATg                                                                                       >  2:2301307/1‑150 (MQ=255)
                                                                aaaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                     <  1:2805776/150‑1 (MQ=255)
                                                                 aaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTc                                                                                    >  1:2397024/1‑150 (MQ=255)
                                                                    cAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 <  1:5667977/150‑1 (MQ=255)
                                                                     aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTa                                                                                >  1:2010313/1‑150 (MQ=255)
                                                                       cGCAGCTTCTCGACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACAGGCGCAGGAAGAAATAGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGGTCGCCCACCATGGTCATTAcg                                                                              <  2:288865/150‑1 (MQ=255)
                                                                           gCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgccgc                                                                          >  2:2239761/1‑150 (MQ=255)
                                                                             ttCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCt                                                                        >  1:440441/1‑150 (MQ=255)
                                                                              tctcTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTa                                                                       >  2:4547620/1‑150 (MQ=255)
                                                                                tctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAAt                                                                     <  2:2363818/150‑1 (MQ=255)
                                                                                   aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCg                                                                  >  1:483246/1‑150 (MQ=255)
                                                                                   aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCg                                                                  <  2:5623133/150‑1 (MQ=255)
                                                                                   aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCg                                                                  >  1:2139970/1‑150 (MQ=255)
                                                                                    ccAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCgg                                                                 <  2:1606604/150‑1 (MQ=255)
                                                                                     cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGt                                                                <  1:4271072/150‑1 (MQ=255)
                                                                                      agcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTg                                                               >  1:3004351/1‑150 (MQ=255)
                                                                                        caggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGcc                                                             >  2:4924453/1‑150 (MQ=255)
                                                                                            caggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGaca                                                         >  2:840897/1‑150 (MQ=255)
                                                                                             aggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGacac                                                        <  1:798912/150‑1 (MQ=255)
                                                                                              ggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACAcc                                                       >  2:5307855/1‑150 (MQ=255)
                                                                                                  aaCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                   >  1:1803879/1‑150 (MQ=255)
                                                                                                  aaCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                   <  1:1925872/150‑1 (MQ=255)
                                                                                                   aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgcat                                                  >  1:2691977/1‑150 (MQ=255)
                                                                                                            aaaTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCAtcatca                                         <  2:595925/150‑1 (MQ=255)
                                                                                                            aaaTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCAtcatca                                         >  2:2058905/1‑150 (MQ=255)
                                                                                                             aaTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAg                                        >  2:5482407/1‑150 (MQ=255)
                                                                                                              aTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAgg                                       <  1:2461502/150‑1 (MQ=255)
                                                                                                                cAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCt                                     >  2:924844/1‑150 (MQ=255)
                                                                                                                  gAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTAc                                   <  2:1717154/150‑1 (MQ=255)
                                                                                                                   aaCCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACg                                  >  2:4865374/1‑150 (MQ=255)
                                                                                                                     ccGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCa                                <  1:2264066/150‑1 (MQ=255)
                                                                                                                        gAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGaa                             <  1:2302468/150‑1 (MQ=255)
                                                                                                                            ggTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGcc                         >  2:1130241/1‑150 (MQ=255)
                                                                                                                            ggTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGcc                         >  2:4330543/1‑150 (MQ=255)
                                                                                                                                  cGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGaa                   >  2:3433593/1‑150 (MQ=255)
                                                                                                                                  cGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGaa                   <  1:3617662/150‑1 (MQ=255)
                                                                                                                                   ggCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAAc                  >  1:3278559/1‑150 (MQ=255)
                                                                                                                                   ggCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAAc                  >  1:3512865/1‑150 (MQ=255)
                                                                                                                                       aCATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGtt              <  1:386889/150‑1 (MQ=255)
                                                                                                                                       aCATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGtt              >  1:2929855/1‑150 (MQ=255)
                                                                                                                                        cATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             <  2:2465185/150‑1 (MQ=255)
                                                                                                                                              cAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAc       >  1:3208357/1‑150 (MQ=255)
                                                                                                                                              cAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAc       >  1:3999900/1‑150 (MQ=255)
                                                                                                                                               aGGAAGAAATCGCCAAACTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc      <  2:1979880/150‑1 (MQ=255)
                                                                                                                                               aGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc      >  2:4196167/1‑150 (MQ=255)
                                                                                                                                                ggAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCa     >  1:856519/1‑150 (MQ=255)
                                                                                                                                                 gaagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCAc    <  1:3581360/150‑1 (MQ=255)
                                                                                                                                                  aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGACCTGTTCCAGACCCACt   >  2:5496187/1‑150 (MQ=255)
                                                                                                                                                  aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACt   >  2:1418866/1‑150 (MQ=255)
                                                                                                                                                  aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACt   <  2:281180/150‑1 (MQ=255)
                                                                                                                                                  aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACt   >  2:2734659/1‑150 (MQ=255)
                                                                                                                                                   agaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACtt  >  1:4064785/1‑150 (MQ=255)
                                                                                                                                                   agaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACtt  <  2:4781339/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTT  >  CP009273/1229312‑1229608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑