BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,342,794	C→T	5.2%	D126N (GAC→AAC)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,342,794	0	C	T	5.2%	1178.9 / 33.3	324	D126N (GAC→AAC)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand): ref base C (153/154); new base T (10/7); total (163/161)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 327 total aligned reads displayed.

TGGTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCT  >  CP009273/1342645‑1342942

tGGTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTc                                                                                                                                                      >  1:927835/1‑150 (MQ=255)

tGGTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTc                                                                                                                                                      <  1:1902325/150‑1 (MQ=255)

 ggTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCg                                                                                                                                                     >  1:4624037/1‑150 (MQ=255)

  gTGCTTCTTTTTCCAGATTATTGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGc                                                                                                                                                    <  2:2182225/150‑1 (MQ=255)

  gTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATTTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGc                                                                                                                                                    >  2:1870246/1‑150 (MQ=255)

     cttcttTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCctt                                                                                                                                                 >  1:5140219/1‑150 (MQ=255)

     cttcttTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCctt                                                                                                                                                 <  1:3251373/150‑1 (MQ=255)

      ttcttTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCcttc                                                                                                                                                >  1:4168029/1‑150 (MQ=255)

       tcttTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCcttct                                                                                                                                               >  1:1800111/1‑150 (MQ=255)

            ttCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTaaa                                                                                                                                          >  1:5429301/1‑150 (MQ=255)

            ttCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTaaa                                                                                                                                          <  2:2328711/150‑1 (MQ=255)

            ttCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTaaa                                                                                                                                          <  1:1440746/150‑1 (MQ=255)

             tCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAAc                                                                                                                                         >  1:2838741/1‑150 (MQ=255)

                aGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCg                                                                                                                                      >  2:3932185/1‑150 (MQ=255)

                  aTTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTg                                                                                                                                    <  2:2514252/150‑1 (MQ=255)

                  aTTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAATTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTg                                                                                                                                    <  2:1564115/150‑1 (MQ=255)

                      tGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCTGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTc                                                                                                                                >  2:787138/1‑150 (MQ=255)

                       ggCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCa                                                                                                                               <  2:3491576/150‑1 (MQ=255)

                          gTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGc                                                                                                                            <  1:5454593/150‑1 (MQ=255)

                           tGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGcc                                                                                                                           >  1:2400286/1‑150 (MQ=255)

                           tGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGcc                                                                                                                           <  2:3189470/150‑1 (MQ=255)

                           tGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGcc                                                                                                                           <  1:4124110/150‑1 (MQ=255)

                             cAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCAc                                                                                                                         <  1:1140143/150‑1 (MQ=255)

                              aaGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACg                                                                                                                        >  2:4647700/1‑150 (MQ=255)

                              aaGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACg                                                                                                                        >  1:3534086/1‑150 (MQ=255)

                              aaGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACg                                                                                                                        >  1:683494/1‑150 (MQ=255)

                                 ggTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGc                                                                                                                     >  1:2542783/1‑150 (MQ=255)

                                  gTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCt                                                                                                                    <  1:449761/150‑1 (MQ=255)

                                   tAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTg                                                                                                                   >  2:5554563/1‑150 (MQ=255)

                                     aCCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTgcg                                                                                                                 <  1:5586899/150‑1 (MQ=255)

                                       ccaccaCGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGcgcg                                                                                                               <  1:2779006/150‑1 (MQ=255)

                                        caccacGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCgg                                                                                                              <  2:5329036/150‑1 (MQ=255)

                                         accacGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGc                                                                                                             >  1:1532449/1‑150 (MQ=255)

                                          ccacGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCa                                                                                                            <  2:2282738/150‑1 (MQ=255)

                                              ggTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGa                                                                                                        >  2:301510/1‑150 (MQ=255)

                                              ggTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGa                                                                                                        >  2:1133944/1‑150 (MQ=255)

                                                 aCAAAGTGATCGTCACCAAACGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGCTGACGGCGCCTGTCGGCAACCGCCCCGTCGCCGTCTTTAAACTCGTGGGTCAGGCCACGGGCTGCGCGGCAAGGAATg                                                                                                     <  2:2714668/150‑1 (MQ=255)

                                                 aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATg                                                                                                     >  2:3341331/1‑150 (MQ=255)

                                                 aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATg                                                                                                     >  2:5558861/1‑150 (MQ=255)

                                                 aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATg                                                                                                     >  2:5192689/1‑150 (MQ=255)

                                                    acgTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTGTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCg                                                                                                  <  2:4676035/148‑1 (MQ=255)

                                                     aGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGt                                                                                                 >  1:4734148/1‑150 (MQ=255)

                                                       tGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCt                                                                                               <  1:1023253/150‑1 (MQ=255)

                                                           cGTCACCAAAAGTGATGTATTGTTTCAGTTCTGCATAGAAAGAACGATAGCCTTTCAGCGGATGACGGCGCATTTCTGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGAAACGTGCTGAGCTGCAAGGAATGGAGTATTTTa                                                                                           >  1:4156445/1‑150 (MQ=255)

                                                             tCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAag                                                                                         >  2:2475669/1‑150 (MQ=255)

                                                             tCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAag                                                                                         >  2:2080600/1‑150 (MQ=255)

                                                                ccAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGt                                                                                      <  1:2240028/150‑1 (MQ=255)

                                                                 cAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGc                                                                                                   >  2:3411076/1‑136 (MQ=255)

                                                                  aaaaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTgg                                                                                    <  1:2918648/150‑1 (MQ=255)

                                                                   aaaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGga                                                                                   <  2:4502305/150‑1 (MQ=255)

                                                                   aaaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGga                                                                                   >  2:5359465/1‑150 (MQ=255)

                                                                     aGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatg                                                                                 >  2:1352078/1‑150 (MQ=255)

                                                                     aGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatg                                                                                 <  2:1397719/150‑1 (MQ=255)

                                                                      gTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatga                                                                                >  2:3614597/1‑150 (MQ=255)

                                                                       tGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatgat                                                                               <  1:385572/150‑1 (MQ=255)

                                                                              ttGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAAc                                                                        >  1:5328373/1‑150 (MQ=255)

                                                                              ttGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAAc                                                                        <  1:1212722/150‑1 (MQ=255)

                                                                               tgtgTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGCGGATGATCAGGAACg                                                                       >  2:877308/1‑150 (MQ=255)

                                                                                gtgtCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGa                                                                      >  1:5014111/1‑150 (MQ=255)

                                                                                 tgtCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGAt                                                                     <  1:72557/150‑1 (MQ=255)

                                                                                 tgtCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGAt                                                                     <  2:4118748/150‑1 (MQ=255)

                                                                                     aGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGcc                                                                 >  2:2057151/1‑150 (MQ=255)

                                                                                     aGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGcc                                                                 >  2:4846217/1‑150 (MQ=255)

                                                                                     aGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTGTTAAGAGTGGATGATCAGGAACGATGGcc                                                                 >  1:2092549/1‑150 (MQ=255)

                                                                                         cTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGAc                                                             <  1:1478601/150‑1 (MQ=255)

                                                                                         cTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGAc                                                             >  1:2659852/1‑150 (MQ=255)

                                                                                         cTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGAc                                                             <  1:4576039/150‑1 (MQ=255)

                                                                                             aTAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCCGACGGTc                                                         >  2:3970420/1‑150 (MQ=255)

                                                                                              tAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCa                                                        <  2:1819383/150‑1 (MQ=255)

                                                                                               agaaagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAt                                                       >  2:5575924/1‑150 (MQ=255)

                                                                                               agaaagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAt                                                       <  2:3896787/150‑1 (MQ=255)

                                                                                                gaaagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAtt                                                      <  1:1085042/150‑1 (MQ=255)

                                                                                                gaaagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAtt                                                      >  1:3167225/1‑150 (MQ=255)

                                                                                                 aaagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAttt                                                     >  1:1484092/1‑150 (MQ=255)

                                                                                                  aagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAtttt                                                    <  2:846457/150‑1 (MQ=255)

                                                                                                    gaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTg                                                  <  1:2909062/150‑1 (MQ=255)

                                                                                                     aaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGc                                                 >  1:3633274/1‑150 (MQ=255)

                                                                                                         aTCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTg                                             >  2:3262483/1‑150 (MQ=255)

                                                                                                         aTCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTg                                             <  1:4846608/150‑1 (MQ=255)

                                                                                                                 tCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTCAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGACTGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTAc                                     >  2:126013/1‑150 (MQ=255)

                                                                                                                 tCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTAc                                     <  1:5289035/150‑1 (MQ=255)

                                                                                                                  caccgtaTGACGGAGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGTATCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTAAAGATGTGATGATCATGAACGAATGCCAGACTCTCATTATTGCCCTGAACCTTAca                                    >  2:432782/7‑149 (MQ=255)

                                                                                                                     cGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCAc                                 <  2:1195966/150‑1 (MQ=255)

                                                                                                                     cGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCAc                                 >  2:2765528/1‑150 (MQ=255)

                                                                                                                           aCGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACg                           >  1:5556459/1‑150 (MQ=255)

                                                                                                                            cGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGa                          >  2:2633271/1‑150 (MQ=255)

                                                                                                                             ggCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAg                         >  1:4343474/1‑150 (MQ=255)

                                                                                                                             ggCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAg                         >  2:3759597/1‑150 (MQ=255)

                                                                                                                              gcgcATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACTAAACGAGt                        <  2:1922697/150‑1 (MQ=255)

                                                                                                                                 cATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGAAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAg                     >  1:5094412/1‑150 (MQ=255)

                                                                                                                                  aTTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAgg                    >  2:489555/1‑150 (MQ=255)

                                                                                                                                    ttCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGaa                  <  1:2736689/150‑1 (MQ=255)

                                                                                                                                     tCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAAc                 <  2:4189697/150‑1 (MQ=255)

                                                                                                                                     tCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAAc                 <  1:19478/150‑1 (MQ=255)

                                                                                                                                     tCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAAc                 <  1:3412367/150‑1 (MQ=255)

                                                                                                                                          aaCCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTc            <  1:3348428/150‑1 (MQ=255)

                                                                                                                                            ccGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCaa          >  1:4245536/1‑150 (MQ=255)

                                                                                                                                               cccAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCa       <  2:3664336/150‑1 (MQ=255)

                                                                                                                                                    tCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGttct  <  1:87960/150‑1 (MQ=255)

                                                                                                                                                    tCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGttct  <  2:544383/150‑1 (MQ=255)

TGGTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCT  >  CP009273/1342645‑1342942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`