BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,454,297	T→G	64.9%	E39D (GAA→GAC)	yfcZ ←	UPF0381 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,454,297	0	T	G	64.9%	581.4 / inf	374	E39D (GAA→GAC)	yfcZ	UPF0381 family protein
Reads supporting (aligned to +/- strand): ref base T (81/50); new base G (127/115); total (209/165)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGG  >  CP009273/2454148‑2454443

    tCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTg                                                                                                                                                <  2:1224265/150‑1 (MQ=255)

    tCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTg                                                                                                                                                <  1:133641/150‑1 (MQ=255)

     cAGCATTACAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTgc                                                                                                                                               <  1:5370273/150‑1 (MQ=255)

       gCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGTTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCAGCCAGCGTTTGCTCTGCTTCTgcgc                                                                                                                                             >  2:3871955/1‑150 (MQ=255)

       gCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTgcgc                                                                                                                                             >  2:266118/1‑150 (MQ=255)

        cATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGTTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGcgcg                                                                                                                                            >  2:1719959/1‑150 (MQ=255)

        cATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGcgcg                                                                                                                                            <  1:2901174/150‑1 (MQ=255)

            tCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGttt                                                                                                                                        <  2:900484/150‑1 (MQ=255)

             cAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTg                                                                                                                                       >  2:3164844/1‑150 (MQ=255)

               gCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCa                                                                                                                                     >  1:3737349/1‑150 (MQ=255)

               gCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCa                                                                                                                                     <  1:1201942/150‑1 (MQ=255)

                   cGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAaca                                                                                                                                 <  2:673642/150‑1 (MQ=255)

                      aGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACAcgc                                                                                                                              >  2:3029515/1‑150 (MQ=255)

                       ggCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgcg                                                                                                                             <  1:963920/150‑1 (MQ=255)

                          aaaaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCt                                                                                                                          >  2:2831381/1‑150 (MQ=255)

                           aaaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATGTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTGTGCTCTGCTTCTGCGCGGTTTGCACACACGCGGCTg                                                                                                                         >  1:4821306/1‑150 (MQ=255)

                            aaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGt                                                                                                                        <  1:1573483/150‑1 (MQ=255)

                               tGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAg                                                                                                                     >  1:5510763/1‑150 (MQ=255)

                               tGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAg                                                                                                                     >  1:2700743/1‑150 (MQ=255)

                                gAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGa                                                                                                                    <  2:4508160/150‑1 (MQ=255)

                                 aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAc                                                                                                                   <  1:5621243/150‑1 (MQ=255)

                                 aaaTCGATATCCAGGCGCACACCGTCAGACTCGTCAGTGAAAGTCGGGGTGATTGTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAc                                                                                                                   <  2:3864080/150‑1 (MQ=255)

                                  aaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAca                                                                                                                  >  2:5567897/1‑149 (MQ=255)

                                     cGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCgg                                                                                                               >  2:4935308/1‑150 (MQ=255)

                                       atatCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTg                                                                                                             <  2:4864408/150‑1 (MQ=255)

                                          tCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAg                                                                                                          >  1:1417467/1‑150 (MQ=255)

                                          tCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAg                                                                                                          <  1:4398657/150‑1 (MQ=255)

                                           ccAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGt                                                                                                         >  2:3597083/1‑150 (MQ=255)

                                             aGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                       <  2:417042/150‑1 (MQ=255)

                                             aGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                       >  1:417030/1‑150 (MQ=255)

                                             aGGCGCACACCGTCACACTCTTCAGTGACAGTCGGGGTGATTTTGCAAGGTTCAGAGTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                       <  2:5298074/150‑1 (MQ=255)

                                                cgcACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAg                                                                                                    >  1:107391/1‑150 (MQ=255)

                                                  cacaCCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGtt                                                                                                  >  1:1813632/1‑150 (MQ=255)

                                                  cacaCCGTCAGACTCTTCAGGGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTGTTCAGGCAGCGCCGCCAGCGGTTGCTCTGCTTCTGCGCGGTGTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGtg                                                                                                  >  1:4355786/1‑149 (MQ=255)

                                                   acacCTTCAGACTCTTCAGTGATAGTCGGGGTGTTTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTg                                                                                                 <  2:4210594/150‑1 (MQ=255)

                                                   acacCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTg                                                                                                 <  1:4641087/150‑1 (MQ=255)

                                                       cGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCa                                                                                             >  1:5116163/1‑150 (MQ=255)

                                                        gTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCAt                                                                                            >  1:31974/1‑150 (MQ=255)

                                                        gTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCAt                                                                                            >  2:4453190/1‑150 (MQ=255)

                                                         tCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATa                                                                                           >  2:4581631/1‑150 (MQ=255)

                                                          cAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATaa                                                                                          >  1:3817529/1‑150 (MQ=255)

                                                          cAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATaa                                                                                          >  1:3268065/1‑150 (MQ=255)

                                                          cAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATaa                                                                                          >  1:3817329/1‑150 (MQ=255)

                                                           aGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAt                                                                                         >  2:3426079/1‑150 (MQ=255)

                                                           aGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAt                                                                                         >  2:2768179/1‑150 (MQ=255)

                                                           aGACACTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCTGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAt                                                                                         <  1:4796250/150‑1 (MQ=255)

                                                               tctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTg                                                                                     >  2:5407137/1‑150 (MQ=255)

                                                                ctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGc                                                                                    >  2:2087492/1‑150 (MQ=255)

                                                                ctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGc                                                                                    >  1:3253040/1‑150 (MQ=255)

                                                                 ttCAGTGAAAGTCGGGGTGATTTTTCAAGGTTCAGATACCACGCAACGGGCTTTTTCAGACAGCGCCGCCAGCGATTGAACTGCGACTGCACGGTTTGCAAACACGAGGCACAAAGAAGCGGTGCACTCGGACATATCCAAAATGGAGcc                                                                                   >  2:3499124/1‑150 (MQ=255)

                                                                 ttCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGcc                                                                                   <  2:532731/150‑1 (MQ=255)

                                                                   cAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCaa                                                                                 >  1:1806247/1‑150 (MQ=255)

                                                                    aGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAAc                                                                                >  1:2169207/1‑150 (MQ=255)

                                                                       gAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATc                                                                             <  1:718888/150‑1 (MQ=255)

                                                                        aaaGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATcc                                                                            >  1:1021869/1‑150 (MQ=255)

                                                                         aaGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCa                                                                           >  1:2467697/1‑150 (MQ=255)

                                                                               gggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcagc                                                                     >  2:591498/1‑150 (MQ=255)

                                                                                ggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcagca                                                                    <  2:2263742/150‑1 (MQ=255)

                                                                                  tGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACagcagc                                                                  >  1:3128813/1‑150 (MQ=255)

                                                                                    aTTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                                >  1:2144774/1‑150 (MQ=255)

                                                                                    aTTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                                >  2:1612618/1‑150 (MQ=255)

                                                                                      tttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                                <  2:138947/148‑1 (MQ=255)

                                                                                      tttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                                >  1:138947/1‑148 (MQ=255)

                                                                                          cAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCggg                                                          >  1:2780068/1‑150 (MQ=255)

                                                                                          cAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCggg                                                          >  2:5647879/1‑150 (MQ=255)

                                                                                          cAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCggg                                                          <  1:3011331/150‑1 (MQ=255)

                                                                                           aaGGTTCATATTCCACGCTAATGGCTTTTTCAGTCAGCTCAGCAAGAGATTGCTCTGCGTCTGCGCGGTTTGCAAACACGAGGATGTAAGACGCGGAGCAGTCGGAGTAGTCCAAAATGGTGCCAACATCCATACAGCAGCacacagcga                                                         >  2:3377784/1‑145 (MQ=255)

                                                                                           aaGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCgggg                                                         >  2:5151941/1‑150 (MQ=255)

                                                                                           aaGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCgggg                                                         <  2:4356574/150‑1 (MQ=255)

                                                                                            aGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAATACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGt                                                        >  2:2318551/1‑150 (MQ=255)

                                                                                               ttCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtc                                                     <  1:788314/150‑1 (MQ=255)

                                                                                                tCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtca                                                    <  1:4294572/150‑1 (MQ=255)

                                                                                                 cAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcat                                                   <  2:4983825/150‑1 (MQ=255)

                                                                                                  aGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                                  >  1:1691559/1‑150 (MQ=255)

                                                                                                    aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAg                                                >  1:5249664/1‑150 (MQ=255)

                                                                                                    aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAg                                                >  1:5009870/1‑150 (MQ=255)

                                                                                                    aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAg                                                <  2:1987600/150‑1 (MQ=255)

                                                                                                     ttCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGc                                               <  2:3733988/150‑1 (MQ=255)

                                                                                                        cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACt                                            >  1:2386876/1‑150 (MQ=255)

                                                                                                        cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACt                                            <  1:25677/150‑1 (MQ=255)

                                                                                                        cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACt                                            <  1:2433479/150‑1 (MQ=255)

                                                                                                            cTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAt                                        >  2:2503758/1‑150 (MQ=255)

                                                                                                            cTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGAGGCTGTAAGACGCGGTGAAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAt                                        >  2:5230646/1‑150 (MQ=255)

                                                                                                               cGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                     <  1:3437496/150‑1 (MQ=255)

                                                                                                               cGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                     >  1:330692/1‑150 (MQ=255)

                                                                                                                gggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAc                                    >  1:4949797/1‑150 (MQ=255)

                                                                                                                   cTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGATTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAATCa                                 >  2:4793763/1‑150 (MQ=255)

                                                                                                                       ttCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGt                             <  1:3597083/150‑1 (MQ=255)

                                                                                                                        tcagtcagCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGtt                            <  2:330620/150‑1 (MQ=255)

                                                                                                                         cagtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTg                           >  2:1031872/1‑150 (MQ=255)

                                                                                                                            tcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAtt                        <  2:3979201/150‑1 (MQ=255)

                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                       <  2:766475/150‑1 (MQ=255)

                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                       <  2:2962131/150‑1 (MQ=255)

                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                       <  1:888640/150‑1 (MQ=255)

                                                                                                                                   cgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTg                 <  1:1825011/150‑1 (MQ=255)

                                                                                                                                    gccAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGt                <  2:4103728/150‑1 (MQ=255)

                                                                                                                                    gccAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGt                >  1:5284236/1‑150 (MQ=255)

                                                                                                                                     ccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTa               >  2:4655371/1‑150 (MQ=255)

                                                                                                                                      cAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAt              >  1:4017040/1‑150 (MQ=255)

                                                                                                                                           tttGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCa         <  1:1989022/150‑1 (MQ=255)

AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGG  >  CP009273/2454148‑2454443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`