BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	180,358	A→C	5.3%	N75K (AAT→AAG)	yaeH ←	UPF0325 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	180,358	0	A	C	5.3%	1138.7 / 39.8	340	N75K (AAT→AAG)	yaeH	UPF0325 family protein
Reads supporting (aligned to +/- strand): ref base A (166/153); new base C (9/9); total (176/164)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 348 total aligned reads displayed.

GTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTT  >  CP009273/180210‑180507

gTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGAt                                                                                                                                                      <  2:844500/150‑1 (MQ=255)

gTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGAt                                                                                                                                                      <  1:2005385/150‑1 (MQ=255)

gTTTTTCCAGATCGGATTCAATCTCGATGATCTTATTGGTTACTACTGACTCCATGTTAAGTAAGTCGTCGATGATCTTAAGCTTAATATCAACTTAGAAGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGAAATACCGTatat                                                                                                                                                      >  1:2233755/1‑150 (MQ=255)

 tttttCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAAGGATATACCGTAGAtt                                                                                                                                                     >  2:1097838/1‑150 (MQ=255)

  ttttCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTc                                                                                                                                                    <  2:4926724/150‑1 (MQ=255)

  ttttCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGAGCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTc                                                                                                                                                    <  2:4133414/150‑1 (MQ=255)

   tttCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCg                                                                                                                                                   >  2:3892201/1‑150 (MQ=255)

       cAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCt                                                                                                                                               <  2:1989845/150‑1 (MQ=255)

        aGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTg                                                                                                                                              >  1:3962534/1‑150 (MQ=255)

           tCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGAtt                                                                                                                                           <  1:4332552/150‑1 (MQ=255)

           tCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGAtt                                                                                                                                           <  1:2558989/150‑1 (MQ=255)

            cGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGAttt                                                                                                                                          >  1:5464100/1‑150 (MQ=255)

                 tCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTgg                                                                                                                                     >  1:3324626/1‑150 (MQ=255)

                      ctcGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGActtc                                                                                                                                <  1:2720999/150‑1 (MQ=255)

                      ctcGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGActtc                                                                                                                                >  1:4500845/1‑150 (MQ=255)

                       tcGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGActtct                                                                                                                               <  2:4332934/150‑1 (MQ=255)

                        cGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGActtctt                                                                                                                              <  2:4238571/150‑1 (MQ=255)

                         gCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCttt                                                                                                                             >  1:2083388/1‑150 (MQ=255)

                         gCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCttt                                                                                                                             >  2:1349487/1‑150 (MQ=255)

                          cTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTta                                                                                                                            >  1:2664865/1‑150 (MQ=255)

                           tGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTtat                                                                                                                           >  1:829853/1‑150 (MQ=255)

                           tGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCGTtat                                                                                                                           >  2:3935328/1‑150 (MQ=255)

                                  aTTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTg                                                                                                                    <  1:3493632/150‑1 (MQ=255)

                                  aTTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTg                                                                                                                    >  2:1153848/1‑150 (MQ=255)

                                   ttGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGa                                                                                                                   <  2:4833670/150‑1 (MQ=255)

                                    tGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGAc                                                                                                                  >  1:726369/1‑150 (MQ=255)

                                         aCGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCcaca                                                                                                             >  1:4159560/1‑150 (MQ=255)

                                           gactgactCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACAcc                                                                                                           >  1:3864078/1‑150 (MQ=255)

                                           gactgactCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACAcc                                                                                                           >  1:5325169/1‑150 (MQ=255)

                                             ctgactCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCAt                                                                                                         <  2:5018743/150‑1 (MQ=255)

                                               gactCCAGGTGACGTAAGTCGTCGAGGATCTTACTCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCa                                                                                                       <  1:1473479/150‑1 (MQ=255)

                                                actCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAg                                                                                                      >  1:158424/1‑150 (MQ=255)

                                                 ctCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGc                                                                                                     <  2:5192525/150‑1 (MQ=255)

                                                    cAGGTGACGTAAGTCGTCGAGTATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCATGATCTTCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTTTAACCCTGACCCACACCATCAGcgac                                                                                                  <  1:1056651/150‑1 (MQ=255)

                                                    cAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGcgac                                                                                                  <  2:959833/150‑1 (MQ=255)

                                                    cAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGcgac                                                                                                  <  1:2191549/150‑1 (MQ=255)

                                                       gTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCgacgac                                                                                               <  2:1763899/150‑1 (MQ=255)

                                                       gTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCgacgac                                                                                               <  2:1525635/150‑1 (MQ=255)

                                                           cGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGtt                                                                                           <  2:1117912/150‑1 (MQ=255)

                                                             tAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGtttt                                                                                         >  1:70585/1‑150 (MQ=255)

                                                             tAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGtttt                                                                                         <  2:4502921/150‑1 (MQ=255)

                                                             tAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGtttt                                                                                         <  2:4617563/150‑1 (MQ=255)

                                                             tAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGtttt                                                                                         >  1:1426494/1‑150 (MQ=255)

                                                                gtcgtcGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACg                                                                                      >  2:3724992/1‑150 (MQ=255)

                                                                        ggATCTTACGCTTAAGATCAACTTCGCTGTGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGAcgcg                                                                              >  1:5068349/1‑150 (MQ=255)

                                                                         gATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCgg                                                                             >  2:4895019/1‑150 (MQ=255)

                                                                          aTCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGCTTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGa                                                                            <  2:5054464/150‑1 (MQ=255)

                                                                              tACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATAtt                                                                        <  1:4365456/150‑1 (MQ=255)

                                                                                 gCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTaa                                                                     <  1:5538059/150‑1 (MQ=255)

                                                                                 gCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTaa                                                                     <  2:2993/150‑1 (MQ=255)

                                                                                   ttAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAAc                                                                   >  1:119448/1‑150 (MQ=255)

                                                                                     aaGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACtt                                                                 <  1:1273894/150‑1 (MQ=255)

                                                                                     aaGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCATTTTACGCTGACGCGGATATTTAAACtt                                                                 <  1:3556070/150‑1 (MQ=255)

                                                                                       gATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTaa                                                               >  2:5424801/1‑150 (MQ=255)

                                                                                       gATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTaa                                                               >  2:2612445/1‑150 (MQ=255)

                                                                                        aTCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAAc                                                              >  1:5387077/1‑150 (MQ=255)

                                                                                        aTCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAAc                                                              >  1:5036225/1‑150 (MQ=255)

                                                                                          cAACTTCGCTGCGGTCACGCTGGCATATTTGATCAAGCTCATCAATGATATACCGTAGCTTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGc                                                            <  2:3405683/150‑1 (MQ=255)

                                                                                           aaCTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGct                                                           <  2:673843/150‑1 (MQ=255)

                                                                                             cTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGctct                                                         <  1:3598991/150‑1 (MQ=255)

                                                                                              ttCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCtt                                                        >  2:2831462/1‑150 (MQ=255)

                                                                                                 gCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGc                                                     <  1:5149997/150‑1 (MQ=255)

                                                                                                  cTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCg                                                    <  2:4987940/150‑1 (MQ=255)

                                                                                                  cTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCg                                                    <  1:3164807/150‑1 (MQ=255)

                                                                                                     cGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGaaa                                                 <  2:5620767/150‑1 (MQ=255)

                                                                                                       gTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGaaaaa                                               <  2:4955854/150‑1 (MQ=255)

                                                                                                            gCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGc                                          <  1:4877201/150‑1 (MQ=255)

                                                                                                               ggCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGGTATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCtt                                       >  2:3662947/1‑150 (MQ=255)

                                                                                                                gCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACTCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCttt                                      <  1:1262696/150‑1 (MQ=255)

                                                                                                                 cAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTg                                     <  2:2705349/150‑1 (MQ=255)

                                                                                                                  aGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGt                                    >  1:169377/1‑150 (MQ=255)

                                                                                                                   gATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTc                                   <  2:576308/150‑1 (MQ=255)

                                                                                                                     tttGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCtt                                 >  1:631252/1‑150 (MQ=255)

                                                                                                                      ttGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCttt                                >  1:4015081/1‑150 (MQ=255)

                                                                                                                        gATCAAGCTCATCAATGATATACCGTAGCTTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCt                              >  2:4037768/1‑150 (MQ=255)

                                                                                                                        gATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCt                              <  2:4844360/150‑1 (MQ=255)

                                                                                                                         aTCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTg                             >  1:1032283/1‑150 (MQ=255)

                                                                                                                           cAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGa                           >  1:28254/1‑150 (MQ=255)

                                                                                                                            aaGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGaa                          <  2:70585/150‑1 (MQ=255)

                                                                                                                            aaGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGaa                          <  1:456410/150‑1 (MQ=255)

                                                                                                                              gCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAAt                        >  1:366739/1‑150 (MQ=255)

                                                                                                                              gCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAAt                        <  2:3098557/150‑1 (MQ=255)

                                                                                                                                 catcaATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGa                     <  2:2229817/150‑1 (MQ=255)

                                                                                                                                  atcaATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGAt                    >  2:4764916/1‑150 (MQ=255)

                                                                                                                                  atcaATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGAt                    >  1:5106367/1‑150 (MQ=255)

                                                                                                                                    caATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAAt                        <  1:2754416/144‑1 (MQ=255)

                                                                                                                                    caATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGAttt                  >  2:587678/1‑150 (MQ=255)

                                                                                                                                      aTGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTc                >  2:3570117/1‑150 (MQ=255)

                                                                                                                                             aCCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAatat         <  1:984886/150‑1 (MQ=255)

                                                                                                                                              ccGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATc        >  2:2117142/1‑150 (MQ=255)

                                                                                                                                              ccGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATc        <  2:2516179/150‑1 (MQ=255)

                                                                                                                                                gTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAACTAGATTTTCAGAATATCgt      >  2:4079576/1‑150 (MQ=255)

                                                                                                                                                 tAGCTTCGGGCTGATTTCCTGGACTTCTTTATACCCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTACACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt     <  2:3242908/150‑1 (MQ=255)

                                                                                                                                                 tAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgtt     >  1:3742860/1‑150 (MQ=255)

                                                                                                                                                  aGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttg    >  2:51430/1‑150 (MQ=255)

                                                                                                                                                  aGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttg    <  2:4398582/150‑1 (MQ=255)

                                                                                                                                                   gATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgg   >  1:4720703/1‑149 (MQ=255)

                                                                                                                                                    aTTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgtt  >  1:2500503/1‑150 (MQ=255)

                                                                                                                                                    aTTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCgttgtt  >  2:2379648/1‑150 (MQ=255)

                                                                                                                                                    aTTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCggtgtt  >  1:5316678/1‑150 (MQ=255)

GTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCTGGCAGATTTGATCAAGCTCATCAATGATATACCGTAGATTCGGGCTGATTTCCTGGACTTCTTTATAACCCTGACCCACACCATCAGCGACGACCGTTTTACGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTT  >  CP009273/180210‑180507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`