Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,778,307 C→T 6.1% pseudogene (64/1374 nt) ypjC ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,778,3070CT 6.1% 1048.9 / 37.2 300pseudogene (64/1374 nt)ypjCpseudogene
Reads supporting (aligned to +/- strand):  ref base C (128/152);  new base T (8/10);  total (137/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 303 total aligned reads displayed.

AAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCATTTGAGACAAT  >  CP009273/2778158‑2778455
                                                                                                                                                     |                                                                                                                                                    
aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                                                      <  2:237810/150‑1 (MQ=255)
  aGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCAt                                                                                                                                                    >  2:915370/1‑150 (MQ=255)
   ggCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCAtt                                                                                                                                                   <  1:2552300/150‑1 (MQ=255)
   ggCGTTGCCATCATGATATATACATTCAGACATCATTATTTGTAAAGTTACAAGAGCACCGCGATACGTCTCTGATGCATCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTTTTTAGAAGATTTATTTTCAtt                                                                                                                                                   <  2:3156556/150‑1 (MQ=255)
     cGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtat                                                                                                                                                 <  1:1153113/150‑1 (MQ=255)
     cGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtat                                                                                                                                                 >  2:4837597/1‑150 (MQ=255)
       ttGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATaa                                                                                                                                               <  1:4540356/150‑1 (MQ=255)
        tGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAAc                                                                                                                                              >  2:4937469/1‑150 (MQ=255)
           ctacatGATATATACATTCAGACATCATATTTTGTAAAGTTTCAAGAGCACCGCGAAACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTACAAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTc                                                                                                                                           >  2:4559027/6‑150 (MQ=255)
             tcttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCa                                                                                                                                         <  1:1074874/150‑1 (MQ=255)
              cttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCAc                                                                                                                                        >  2:1218395/1‑150 (MQ=255)
               ttGATTTATACATTCAGTCATCATTTTTTGTAAAGTTTCAATAGCACAGAGATACGTCTCTTATTCAGCTTCTTTACTAATTAATTTTAAAATACTATGATCTAGTAGATCCATGTTTTTAGAAGATTTATTTTGATTATTACTTCCACt                                                                                                                                       <  2:3106220/150‑1 (MQ=255)
                  atatatACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTAcc                                                                                                                                    >  1:5325599/1‑150 (MQ=255)
                   tatataCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTAccc                                                                                                                                   >  1:4319583/1‑150 (MQ=255)
                    atataCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCa                                                                                                                                  <  1:1046476/150‑1 (MQ=255)
                    atataCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCa                                                                                                                                  >  2:3953644/1‑150 (MQ=255)
                     tataCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCaa                                                                                                                                 >  2:656024/1‑150 (MQ=255)
                        aCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTATCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAAc                                                                                                                              <  2:4626929/150‑1 (MQ=255)
                         cATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTTATTATAACTTCCACTACCCAAAACt                                                                                                                             <  1:5654784/150‑1 (MQ=255)
                         cATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACt                                                                                                                             >  2:4177567/1‑150 (MQ=255)
                          aTTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTg                                                                                                                            >  1:3958052/1‑150 (MQ=255)
                             cAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTgcgc                                                                                                                         >  1:410371/1‑150 (MQ=255)
                                  atcatTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCGCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAACACTGCGCTAGCg                                                                                                                    <  2:347092/150‑1 (MQ=255)
                                       tttttGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCGAGCGTTaaa                                                                                                               <  1:4199494/150‑1 (MQ=255)
                                           tGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCGTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCGAGCGTTAAATCCg                                                                                                           <  1:4710851/150‑1 (MQ=255)
                                             tAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGtt                                                                                                         >  2:459065/1‑150 (MQ=255)
                                               aaGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGc                                                                                                       <  1:2171184/150‑1 (MQ=255)
                                                aGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCt                                                                                                      <  2:2667845/150‑1 (MQ=255)
                                                 gTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCtt                                                                                                     >  2:1741833/1‑150 (MQ=255)
                                                  tttCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCttt                                                                                                    <  1:308698/150‑1 (MQ=255)
                                                  tttCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCttt                                                                                                    <  1:2614212/150‑1 (MQ=255)
                                                   ttCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCtttt                                                                                                   <  1:5015577/150‑1 (MQ=255)
                                                    tCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTa                                                                                                  >  1:4801947/1‑150 (MQ=255)
                                                    tCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTa                                                                                                  >  1:4033024/1‑150 (MQ=255)
                                                      aaGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTact                                                                                                >  2:2766652/1‑150 (MQ=255)
                                                      aaGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTact                                                                                                >  1:215566/1‑150 (MQ=255)
                                                       agagCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTacta                                                                                               >  2:5102379/1‑150 (MQ=255)
                                                          gCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTactaact                                                                                            <  1:60134/150‑1 (MQ=255)
                                                           cACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTTATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTactaacta                                                                                           >  1:1414040/1‑150 (MQ=255)
                                                            aCCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTActaactaa                                                                                          >  1:205080/1‑150 (MQ=255)
                                                               gcgATACGTCTCTGATGCAGCTTCCTTATAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACAt                                                                                       <  2:2193453/150‑1 (MQ=255)
                                                               gcgATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACAt                                                                                       <  1:1975491/150‑1 (MQ=255)
                                                               gcgATACGTCTCTGACGCAGCATCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTTTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACAt                                                                                       <  2:3029484/150‑1 (MQ=255)
                                                                    aCGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTTATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCt                                                                                  <  1:4327697/150‑1 (MQ=255)
                                                                    aCGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCt                                                                                  >  2:1088422/1‑150 (MQ=255)
                                                                     cGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTa                                                                                 >  2:1893244/1‑150 (MQ=255)
                                                                     cGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTa                                                                                 >  1:3324360/1‑150 (MQ=255)
                                                                      gTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAt                                                                                >  1:2043049/1‑150 (MQ=255)
                                                                      gTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAt                                                                                >  2:3313313/1‑150 (MQ=255)
                                                                      gTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAt                                                                                <  1:646498/150‑1 (MQ=255)
                                                                      gTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAt                                                                                >  2:4464553/1‑150 (MQ=255)
                                                                       tctcTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAtt                                                                               <  1:2665304/150‑1 (MQ=255)
                                                                         tctGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTcc                                                                             <  2:3216914/150‑1 (MQ=255)
                                                                         tatGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCAATGTGTTTATAATATTTATTTTAATTATAACTTCCACTACCCAAAACTGCGCTAGCGTAAAAAACGTTGCTATTACTAAATAACATTGTCTATTcc                                                                             >  1:2960988/3‑150 (MQ=255)
                                                                             aTGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCGAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCaa                                                                         <  1:3520489/150‑1 (MQ=255)
                                                                              tGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAAt                                                                        >  2:3684104/1‑150 (MQ=255)
                                                                                   cTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTTATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATg                                                                   >  1:3240512/1‑150 (MQ=255)
                                                                                    ttCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTCATGt                                                                  >  2:1031804/1‑150 (MQ=255)
                                                                                     tCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTc                                                                 <  1:4414182/150‑1 (MQ=255)
                                                                                            aaaTTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTTATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATgg                                                          <  2:3770820/150‑1 (MQ=255)
                                                                                             aattaattTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGc                                                         <  2:5437182/150‑1 (MQ=255)
                                                                                             aattaattTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGc                                                         <  2:2000122/150‑1 (MQ=255)
                                                                                                taattTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAt                                                      <  2:5614812/150‑1 (MQ=255)
                                                                                                taattTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAt                                                      <  1:5254802/150‑1 (MQ=255)
                                                                                                taattTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAt                                                      >  2:1826528/1‑150 (MQ=255)
                                                                                                  attTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAttt                                                    <  2:2641493/150‑1 (MQ=255)
                                                                                                  attTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAttt                                                    <  2:5225570/150‑1 (MQ=255)
                                                                                                   ttttAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAtttt                                                   <  2:290952/150‑1 (MQ=255)
                                                                                                    tttAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGGCTATTCCTCATTTAATGTCTACATGGCTAtttgt                                                  >  1:3810918/1‑148 (MQ=255)
                                                                                                      tAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTaa                                                >  2:2341657/1‑150 (MQ=255)
                                                                                                        aaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATg                                              <  1:275819/150‑1 (MQ=255)
                                                                                                         aaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGt                                             >  2:2016685/1‑150 (MQ=255)
                                                                                                              tATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGttatta                                        >  1:2353791/1‑150 (MQ=255)
                                                                                                              tATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGttatta                                        <  2:2517124/150‑1 (MQ=255)
                                                                                                               aTGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTAc                                       <  2:431992/150‑1 (MQ=255)
                                                                                                                 gAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTg                                     <  2:3360028/150‑1 (MQ=255)
                                                                                                                 gAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGATAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTg                                     <  1:1218395/150‑1 (MQ=255)
                                                                                                                   gCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGtt                                   <  2:852459/150‑1 (MQ=255)
                                                                                                                     tagtagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTg                                 >  1:2888579/1‑150 (MQ=255)
                                                                                                                      agtagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGt                                <  1:3143745/150‑1 (MQ=255)
                                                                                                                      agtagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGt                                <  1:3683599/150‑1 (MQ=255)
                                                                                                                        tagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTATACATGGCTATTTTTAATGTTATTACTGTTTTTCa                              >  2:5589279/1‑150 (MQ=255)
                                                                                                                           cTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACtc                           >  2:3081736/1‑149 (MQ=255)
                                                                                                                            tCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACtat                          <  1:459065/150‑1 (MQ=255)
                                                                                                                             ccATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACtata                         <  2:5558278/150‑1 (MQ=255)
                                                                                                                                tgtgTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATaaaa                      >  2:1284513/1‑150 (MQ=255)
                                                                                                                                 gtgtTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATaaaaa                     <  2:5325599/150‑1 (MQ=255)
                                                                                                                                  tgtTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATaaaaaa                    >  1:3490002/1‑150 (MQ=255)
                                                                                                                                  tgtTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATaaaaaa                    <  1:1775802/150‑1 (MQ=255)
                                                                                                                                     ttAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACg                 >  1:5570923/1‑150 (MQ=255)
                                                                                                                                      tAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGc                <  2:410371/150‑1 (MQ=255)
                                                                                                                                        gaagaTTTATTTTCATTATAAATTCCACTACCCAAAACTGCGCTAGCGTTAAAACAGTTGATTTTACTAACTAACATTGACTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTTTCACTATAAAAAAACGCTc              >  2:4495505/1‑150 (MQ=255)
                                                                                                                                         cagaTTTATTTTCATTAGAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCa             <  2:4806348/149‑1 (MQ=255)
                                                                                                                                          agaTTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCAt            <  1:1827140/150‑1 (MQ=255)
                                                                                                                                            atttatttTTATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCAttt          >  1:3851027/1‑150 (MQ=255)
                                                                                                                                            atttatttTTATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCAttt          <  1:3166076/150‑1 (MQ=255)
                                                                                                                                             tttatttTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCATTTg         >  1:1525425/1‑150 (MQ=255)
                                                                                                                                             tttatttTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCATTTg         >  2:823043/1‑150 (MQ=255)
                                                                                                                                               tatttTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCATTTgag       >  2:1646319/1‑150 (MQ=255)
                                                                                                                                                atttTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGATCATTTgaga      >  1:3036773/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTATAAAAAAACGCTCATTTGAGACAAT  >  CP009273/2778158‑2778455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑