Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,774 C→A 17.2% pseudogene (346/447 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,7740CA17.2% 1012.3 / 157.4 354pseudogene (346/447 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base C (184/108);  new base A (31/30);  total (215/139)
Fisher's exact test for biased strand distribution p-value = 8.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 356 total aligned reads displayed.

AATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCAT  >  CP009273/2988625‑2988922
                                                                                                                                                     |                                                                                                                                                    
     tACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTaa                                                                                                                                                 >  2:1557859/1‑150 (MQ=255)
      aCAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAAc                                                                                                                                                >  1:4810362/1‑150 (MQ=255)
      aCAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAAc                                                                                                                                                <  2:1503366/150‑1 (MQ=255)
        aaTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGCGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCt                                                                                                                                              >  1:2559532/1‑150 (MQ=255)
            aGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTc                                                                                                                                          <  2:4617824/150‑1 (MQ=255)
            aGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTACTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTc                                                                                                                                          <  1:1171844/150‑1 (MQ=255)
                tataTTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTAGTTCGCAGATTTCGTTAACCTTGTCAggg                                                                                                                                      >  1:4504293/1‑150 (MQ=255)
                 atatTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgggg                                                                                                                                     >  2:4236069/1‑150 (MQ=255)
                 atatTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAgggg                                                                                                                                     <  1:3167121/150‑1 (MQ=255)
                      cAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGaaa                                                                                                                                >  2:4882369/1‑150 (MQ=255)
                        aaTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACa                                                                                                                              >  2:4087827/1‑150 (MQ=255)
                        aaTCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACa                                                                                                                              <  1:5402705/150‑1 (MQ=255)
                            aTTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGa                                                                                                                          >  2:5075732/1‑150 (MQ=255)
                              tAAACTTGAGCAAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAAAAGAATTATACATATCCAAAGAATGAGTTTCAAACATTTTTTCAACTAAATAATGTCTGTCTTGTTCGCAGATTTAGTTAAACTTGAAAGGGGAGAAAAAATGatt                                                                                                                        >  1:5553207/1‑150 (MQ=255)
                               aaaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGatta                                                                                                                       >  2:5107435/1‑150 (MQ=255)
                               aaaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGatta                                                                                                                       >  1:5325705/1‑150 (MQ=255)
                                aaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                      >  2:263515/1‑150 (MQ=255)
                                aaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                      >  2:2252024/1‑150 (MQ=255)
                                aaCTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGattat                                                                                                                      <  1:4580096/150‑1 (MQ=255)
                                  cTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGAttatta                                                                                                                    >  2:1356011/1‑150 (MQ=255)
                                    tGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTattaat                                                                                                                  <  2:887898/150‑1 (MQ=255)
                                    tGAGACAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTattaat                                                                                                                  <  2:4729248/150‑1 (MQ=255)
                                        ccAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTaaa                                                                                                              <  1:511257/150‑1 (MQ=255)
                                          aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                            <  2:819829/150‑1 (MQ=255)
                                          aGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAata                                                                                                            >  2:5374449/1‑150 (MQ=255)
                                              aGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGc                                                                                                        >  2:1172793/1‑150 (MQ=255)
                                              aGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGc                                                                                                        >  2:2703173/1‑150 (MQ=255)
                                              aGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGc                                                                                                        >  2:5513215/1‑150 (MQ=255)
                                                  ataAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTcac                                                                                                    <  1:3536983/150‑1 (MQ=255)
                                                   taAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTcaca                                                                                                   >  2:1049445/1‑150 (MQ=255)
                                                     aGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAAt                                                                                                 <  1:4205701/150‑1 (MQ=255)
                                                      gTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAAtt                                                                                                <  1:3889947/150‑1 (MQ=255)
                                                           cAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCca                                                                                           >  1:497163/1‑150 (MQ=255)
                                                           cAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCca                                                                                           <  1:2771807/150‑1 (MQ=255)
                                                            aGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCcac                                                                                          >  1:58173/1‑150 (MQ=255)
                                                            aGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCcac                                                                                          <  2:1257156/150‑1 (MQ=255)
                                                                  tGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTc                                                                                    <  1:2419787/150‑1 (MQ=255)
                                                                   gacAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTcc                                                                                   <  2:5393238/148‑1 (MQ=255)
                                                                   gCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTcc                                                                                   >  1:785662/1‑150 (MQ=255)
                                                                   gCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTcc                                                                                   <  2:2237067/150‑1 (MQ=255)
                                                                     cAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTg                                                                                 >  2:2356573/1‑150 (MQ=255)
                                                                       aaaCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCg                                                                               <  1:3476502/150‑1 (MQ=255)
                                                                       aaaCAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCg                                                                               <  1:1662231/150‑1 (MQ=255)
                                                                          cAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGAc                                                                            >  1:3324245/1‑150 (MQ=255)
                                                                             aaTTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTaa                                                                         >  1:4765684/1‑150 (MQ=255)
                                                                              attattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTaaa                                                                        >  1:3703787/1‑150 (MQ=255)
                                                                                tattCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAAtt                                                                      >  2:3098871/1‑150 (MQ=255)
                                                                                    cATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGc                                                                  <  1:617966/150‑1 (MQ=255)
                                                                                       atCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAAt                                                               <  1:1004286/150‑1 (MQ=255)
                                                                                        accAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGTATTGTTCGCAGATTTAGTTAACCTTGACAGGGGAGAAACAATGATTATTAAATAAATATGGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATa                                                              <  2:1860788/149‑1 (MQ=255)
                                                                                         ccAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATaa                                                             <  1:2897183/150‑1 (MQ=255)
                                                                                          cAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATaaa                                                            >  1:144798/1‑150 (MQ=255)
                                                                                           aaaGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAAt                                                           >  1:2056837/1‑150 (MQ=255)
                                                                                              gAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGa                                                        >  2:352902/1‑150 (MQ=255)
                                                                                              gAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGAGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGa                                                        >  1:2627391/1‑150 (MQ=255)
                                                                                                aTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                      >  2:780482/1‑150 (MQ=255)
                                                                                                aTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                      <  1:5075732/150‑1 (MQ=255)
                                                                                                aTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                      <  2:1766220/150‑1 (MQ=255)
                                                                                                aTGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATc                                                      <  2:5163752/150‑1 (MQ=255)
                                                                                                 tgtgTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCg                                                     >  1:4320369/1‑150 (MQ=255)
                                                                                                  gtgtTTCAAACATTTTTTAAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCgg                                                    <  2:3860104/150‑1 (MQ=255)
                                                                                                    gtTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTa                                                  >  1:4089932/1‑150 (MQ=255)
                                                                                                       tCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTAGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCATGCGGACTAAATTCAGCAATAAATTGATCGGTaaaa                                               >  2:333372/1‑150 (MQ=255)
                                                                                                        cAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTaaaaa                                              >  2:5602519/1‑150 (MQ=255)
                                                                                                           aCATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAAGTAAATATTGCTCACTATTATCGACACTTTCCTGCGGAGTAAATTGCGCAAGAAATTGATCGGTaaagcaca                                           >  2:3410648/1‑145 (MQ=255)
                                                                                                            cATTTTTTCAACTAAATAATGTTTGGCTTGTTCGCAGATGTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAtcac                                          >  1:1860160/1‑150 (MQ=255)
                                                                                                            cATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAacac                                          >  2:691017/1‑150 (MQ=255)
                                                                                                             aTTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAAATCCTGCGGACTAAATTCAGCAATAAATTGATCTGTAATAAAcaca                                         >  1:3525628/1‑150 (MQ=255)
                                                                                                                ttttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCTTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACaaaa                                      <  1:669693/150‑1 (MQ=255)
                                                                                                                ttttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACaaaa                                      <  1:1388819/150‑1 (MQ=255)
                                                                                                                 tttCAACTAAATAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAAc                                     <  1:2309366/150‑1 (MQ=255)
                                                                                                                     aaCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCtt                                 <  2:4045124/150‑1 (MQ=255)
                                                                                                                      aCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCTCAACTTCCTGCGGACTAAATTCAGCAATACATTGATCGGTACAAAACACAAAACGCTTa                                >  2:3025281/1‑150 (MQ=255)
                                                                                                                      aCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTa                                >  1:4704219/1‑150 (MQ=255)
                                                                                                                      aCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTa                                >  1:4874857/1‑150 (MQ=255)
                                                                                                                      aCTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTa                                >  1:898475/1‑150 (MQ=255)
                                                                                                                       cTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTat                               >  2:2135598/1‑150 (MQ=255)
                                                                                                                         aaaTAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatca                             >  1:2313046/1‑150 (MQ=255)
                                                                                                                         aaaTAATGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTatca                             >  2:816702/1‑150 (MQ=255)
                                                                                                                            taatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTAtcatca                          >  2:5220104/1‑150 (MQ=255)
                                                                                                                            taatGTCTGGCTTGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTAtcatca                          >  1:5138728/1‑150 (MQ=255)
                                                                                                                             aatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATcatcat                         <  1:999329/150‑1 (MQ=255)
                                                                                                                             aatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATcatcat                         >  2:3625779/1‑150 (MQ=255)
                                                                                                                             aatGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCGTAtcatcag                         >  2:3445747/1‑149 (MQ=255)
                                                                                                                              atGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCAACATg                        >  2:3695408/1‑150 (MQ=255)
                                                                                                                                  cTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGATAAACAATGATTATTAATTAAATATTGCTAAAAATTATCCACAAAATCCTGAGTACTAAATTAAGCAATAAATTGATCGGTAAAAAACACAAAACGATATTCTTCATGAAAt                    >  2:1380727/1‑150 (MQ=255)
                                                                                                                                  cTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCTTCATGAAAt                    >  2:3499868/1‑150 (MQ=255)
                                                                                                                                   tGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATg                   <  2:5656012/150‑1 (MQ=255)
                                                                                                                                     gCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTa                 <  1:5622131/150‑1 (MQ=255)
                                                                                                                                      cTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTaa                <  1:4465448/150‑1 (MQ=255)
                                                                                                                                        tGTTCGCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAata              >  1:1942863/1‑150 (MQ=255)
                                                                                                                                         gTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAatat             <  2:1675657/150‑1 (MQ=255)
                                                                                                                                         gTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAatat             <  1:3025554/150‑1 (MQ=255)
                                                                                                                                         gTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAatat             >  1:638305/1‑150 (MQ=255)
                                                                                                                                          ttCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATg            <  2:3703787/150‑1 (MQ=255)
                                                                                                                                          ttCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGAAATAAATTGATCGGTAAAAAACACAAAACTCTTATCATCATGAAATGTCATATg            >  2:4448094/1‑150 (MQ=255)
                                                                                                                                            cGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGa          >  2:4234584/1‑150 (MQ=255)
                                                                                                                                             gCAGATTTAGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAc         >  1:1670409/1‑150 (MQ=255)
                                                                                                                                               aGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAcct       >  2:5280913/1‑148 (MQ=255)
                                                                                                                                                 aTTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTc     <  2:3931767/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCAT  >  CP009273/2988625‑2988922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑