BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,020,629	C→A	42.3%	I50I (ATC→ATA)	ygfQ →	putative purine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,020,629	0	C	A	42.3%	291.4 / inf	340	I50I (ATC→ATA)	ygfQ	putative purine permease
Reads supporting (aligned to +/- strand): ref base C (89/107); new base A (78/66); total (167/173)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 357 total aligned reads displayed.

ATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACGGCGTT  >  CP009273/3020480‑3020778

    cTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgtcg                                                                                                                                                   <  2:1084636/150‑1 (MQ=255)

       gagaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTc                                                                                                                                                >  1:1011559/1‑150 (MQ=255)

        agaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTcc                                                                                                                                               >  1:3166800/1‑150 (MQ=255)

         gaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCg                                                                                                                                              >  1:4026479/1‑150 (MQ=255)

         gaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCg                                                                                                                                              >  1:251471/1‑150 (MQ=255)

           cATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCggg                                                                                                                                            >  2:2128482/1‑150 (MQ=255)

             tCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGaa                                                                                                                                          <  2:188726/150‑1 (MQ=255)

             tCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGaa                                                                                                                                          <  2:4559304/150‑1 (MQ=255)

              ccTACAAACACCGGACGCACCAAAGCCACAGGGCTCGCAGGATAATTATTTTAAAATTTCCGCTCGTGTCAGTACCGTTCGTCAGGAAGTACTGTCTGGCTTAACGACCTTTCTGTCCATGGTTTATTCCGTTATAGTCGTTCCGGGAAt                                                                                                                                         <  1:3882406/150‑1 (MQ=255)

              ccTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAAt                                                                                                                                         <  2:4788344/150‑1 (MQ=255)

                  cAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTg                                                                                                                                     <  1:3048876/150‑1 (MQ=255)

                         cGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAg                                                                                                                              >  1:342685/1‑150 (MQ=255)

                          ggACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGc                                                                                                                             >  2:359531/1‑150 (MQ=255)

                                aCCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGttt                                                                                                                       >  2:4527592/1‑150 (MQ=255)

                                 ccAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGATGGGCAAAGCAGGtttt                                                                                                                      >  2:4000037/1‑150 (MQ=255)

                                  cAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtc                                                                                                                     >  2:1532442/1‑150 (MQ=255)

                                   aaaGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtcc                                                                                                                    >  1:266824/1‑150 (MQ=255)

                                       ccACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTccc                                                                                                                <  1:720170/150‑1 (MQ=255)

                                        cacaGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCg                                                                                                               <  2:3117930/150‑1 (MQ=255)

                                        cacaGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCg                                                                                                               >  1:1879153/1‑150 (MQ=255)

                                           aGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAg                                                                                                            >  1:5335777/1‑150 (MQ=255)

                                             ggCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCt                                                                                                          <  1:3182396/150‑1 (MQ=255)

                                               cgcgcTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCtgt                                                                                                        <  2:2740545/150‑1 (MQ=255)

                                               cgcgcTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCtgt                                                                                                        <  2:3499730/150‑1 (MQ=255)

                                                 cgcTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTgtgt                                                                                                      <  2:2501198/150‑1 (MQ=255)

                                                    tGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttg                                                                                                   >  1:4090916/1‑150 (MQ=255)

                                                    tGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttg                                                                                                   >  1:4771364/1‑150 (MQ=255)

                                                    tGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttg                                                                                                   >  2:5081157/1‑150 (MQ=255)

                                                        tAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGc                                                                                               >  1:4605456/1‑150 (MQ=255)

                                                        tAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGc                                                                                               >  1:1704289/1‑150 (MQ=255)

                                                        tAATTATTTTAAAATGACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGc                                                                                               <  2:5556645/150‑1 (MQ=255)

                                                            tattTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCAcc                                                                                           <  1:227407/150‑1 (MQ=255)

                                                             attTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCt                                                                                          >  1:1100294/1‑150 (MQ=255)

                                                             attTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTGTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCt                                                                                          >  1:5229209/1‑150 (MQ=255)

                                                                    aaTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGt                                                                                   >  1:2767529/1‑150 (MQ=255)

                                                                     aTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTc                                                                                  >  2:3210428/1‑150 (MQ=255)

                                                                     aTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTc                                                                                  <  2:4283003/150‑1 (MQ=255)

                                                                     aTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTc                                                                                  >  2:497614/1‑150 (MQ=255)

                                                                       tACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTcgc                                                                                >  1:1973299/1‑150 (MQ=255)

                                                                       tACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTcgc                                                                                >  2:5547074/1‑150 (MQ=255)

                                                                          cGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCggg                                                                             >  1:5654215/1‑150 (MQ=255)

                                                                            cTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCt                                                                           <  2:720293/150‑1 (MQ=255)

                                                                              cGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTc                                                                         <  1:1900631/150‑1 (MQ=255)

                                                                               gTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGGTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCg                                                                        <  2:917992/150‑1 (MQ=255)

                                                                               gTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTGTCTGGCCATGGTTTATTCCGTTATAGTAGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCTGGCTTCg                                                                        >  2:1237079/1‑150 (MQ=255)

                                                                                tGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCgg                                                                       >  2:2827047/1‑150 (MQ=255)

                                                                                 ggCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGc                                                                      >  2:5650605/1‑150 (MQ=255)

                                                                                  gCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCt                                                                     <  1:2128482/150‑1 (MQ=255)

                                                                                   cAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTc                                                                    >  1:791290/1‑150 (MQ=255)

                                                                                      tACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGtt                                                                 <  2:516630/150‑1 (MQ=255)

                                                                                         cGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCt                                                              <  1:151067/150‑1 (MQ=255)

                                                                                         cGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCt                                                              >  1:2317658/1‑150 (MQ=255)

                                                                                          gTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTg                                                             >  1:1173852/1‑150 (MQ=255)

                                                                                          gTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTg                                                             >  2:4525084/1‑150 (MQ=255)

                                                                                           ttCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGa                                                            >  1:3292237/1‑150 (MQ=255)

                                                                                            tCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGAt                                                           <  1:2935860/150‑1 (MQ=255)

                                                                                             cGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATg                                                          >  2:2419425/1‑150 (MQ=255)

                                                                                              gTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATgg                                                         <  1:3610272/150‑1 (MQ=255)

                                                                                              gTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATgg                                                         <  1:4851944/150‑1 (MQ=255)

                                                                                              gTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATgg                                                         >  1:219373/1‑150 (MQ=255)

                                                                                                 aGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGAt                                                      <  2:1269477/150‑1 (MQ=255)

                                                                                                     aGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATg                                                  >  2:1152011/1‑150 (MQ=255)

                                                                                                      gTACTGGCTGGCTTAACGACCTTTCTGGCCCTGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATgg                                                 >  1:4955523/1‑150 (MQ=255)

                                                                                                       tACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATggg                                                >  2:2565380/1‑150 (MQ=255)

                                                                                                        aCTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCATTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGc                                               <  2:4449477/150‑1 (MQ=255)

                                                                                                         ctggctggCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCt                                              <  1:4907863/150‑1 (MQ=255)

                                                                                                           ggctggctTAACGACCTTTCGGGCCAGGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCACAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTaa                                            <  2:4865056/150‑1 (MQ=255)

                                                                                                            gctggctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAAt                                           <  2:2328708/150‑1 (MQ=255)

                                                                                                             ctggctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAAtt                                          <  2:4190576/150‑1 (MQ=255)

                                                                                                               ggctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTg                                        <  2:5231716/150‑1 (MQ=255)

                                                                                                                gctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGc                                       <  1:1239827/150‑1 (MQ=255)

                                                                                                                 ctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGcc                                      <  1:3282847/150‑1 (MQ=255)

                                                                                                                   tAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCaa                                    <  2:342685/150‑1 (MQ=255)

                                                                                                                    aaCGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAAt                                   >  1:42117/1‑150 (MQ=255)

                                                                                                                     aCGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATg                                  <  2:1773530/150‑1 (MQ=255)

                                                                                                                       gACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGc                                <  1:3515330/150‑1 (MQ=255)

                                                                                                                           tttCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGAtt                            <  2:1068963/150‑1 (MQ=255)

                                                                                                                           tttCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTTTGTTTGTTGCCACCTGTCTGGTCTCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGAtt                            <  1:4402347/150‑1 (MQ=255)

                                                                                                                             tCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTgg                          <  1:1596921/150‑1 (MQ=255)

                                                                                                                             tCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTgg                          <  1:1436194/150‑1 (MQ=255)

                                                                                                                              cgggCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGt                         <  2:5030158/148‑1 (MQ=255)

                                                                                                                              cTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGt                         >  2:3752821/1‑150 (MQ=255)

                                                                                                                                ggCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTg                       >  2:768303/1‑150 (MQ=255)

                                                                                                                                  ccATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgcg                     >  2:1475243/1‑150 (MQ=255)

                                                                                                                                   cATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgcgc                    >  2:2866276/1‑150 (MQ=255)

                                                                                                                                    aTGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGcgcg                   >  2:2692089/1‑150 (MQ=255)

                                                                                                                                    aTGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGcgcg                   >  2:5419519/1‑150 (MQ=255)

                                                                                                                                      ggTTAATTCCGTTATAGTCGTTACGGGAATGCTGGGCCAAGCAGGTTTTCAACCCGCAGCAGGGTATGTTGCAAACTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGAATGTTTGCGCGAt                 >  2:1961438/1‑150 (MQ=255)

                                                                                                                                        tttATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGAttt               >  1:1528361/1‑150 (MQ=255)

                                                                                                                                           agtCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGTATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCt            <  2:5193972/148‑1 (MQ=255)

                                                                                                                                           aTTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCt            >  1:5449369/1‑150 (MQ=255)

                                                                                                                                            ttCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCtt           <  2:5335565/150‑1 (MQ=255)

                                                                                                                                            ttCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCtt           >  1:3960534/1‑150 (MQ=255)

                                                                                                                                               cGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGAc        <  2:162323/150‑1 (MQ=255)

                                                                                                                                               cGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGAc        <  2:164593/150‑1 (MQ=255)

                                                                                                                                               cGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGAc        <  2:3901383/150‑1 (MQ=255)

                                                                                                                                               cGTTATCGTCGGTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGAc        <  1:647208/150‑1 (MQ=255)

                                                                                                                                               cGTTATCGACGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGAc        <  2:3030555/150‑1 (MQ=255)

                                                                                                                                                gTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACg       >  1:5180654/1‑150 (MQ=255)

                                                                                                                                                 ttATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCACCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATAGGTTTCGCGATTTCCATGACgg      >  1:1855464/1‑150 (MQ=255)

ATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACGGCGTT  >  CP009273/3020480‑3020778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`