BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,024,983	C→G	54.1%	G86G (GGC→GGG)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,024,983	0	C	G	54.1%	135.1 / inf	342	G86G (GGC→GGG)	uacT	uric acid permease
Reads supporting (aligned to +/- strand): ref base C (79/77); new base G (99/86); total (179/163)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

CACGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGC  >  CP009273/3024834‑3025132

  cGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCg                                                                                                                                                     <  1:3458443/150‑1 (MQ=255)

   gTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTAATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGgcgc                                                                                                                                                    >  2:5264954/1‑150 (MQ=255)

        tGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACCTTATTGCAATGTATCGGTATCGGCCGCGTTAt                                                                                                                                               >  1:1100980/1‑150 (MQ=255)

               tACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATc                                                                                                                                        >  1:1059658/1‑150 (MQ=255)

                aCGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAACAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATcc                                                                                                                                       <  2:3894135/150‑1 (MQ=255)

                aCGCAGGTGCAGTCGCTGGTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATcc                                                                                                                                       <  2:4393682/150‑1 (MQ=255)

                    aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCt                                                                                                                                   <  1:1880792/150‑1 (MQ=255)

                    aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAAGTTATCGGTATCGGGCGCTTTATGGGGATACGCCt                                                                                                                                   >  2:5030535/1‑150 (MQ=255)

                     ggTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTTCGATTCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTGTATGGGGATCCGCCTg                                                                                                                                  >  1:2608497/1‑150 (MQ=255)

                     ggTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTg                                                                                                                                  <  2:4890974/150‑1 (MQ=255)

                     ggTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTg                                                                                                                                  <  2:4890871/150‑1 (MQ=255)

                       tGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGcc                                                                                                                                <  2:5473200/150‑1 (MQ=255)

                                tGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTAt                                                                                                                       >  2:5098442/1‑150 (MQ=255)

                                tGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTAt                                                                                                                       <  1:2823618/150‑1 (MQ=255)

                                  ttCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGt                                                                                                                     <  1:1604880/150‑1 (MQ=255)

                                  ttCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGt                                                                                                                     <  2:1606552/150‑1 (MQ=255)

                                  ttCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGt                                                                                                                     <  2:4489696/150‑1 (MQ=255)

                                   tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTc                                                                                                                    <  2:257798/150‑1 (MQ=255)

                                   tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTc                                                                                                                    <  2:627554/150‑1 (MQ=255)

                                   tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTc                                                                                                                    <  2:5437116/150‑1 (MQ=255)

                                     ctctTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCgg                                                                                                                  >  1:4369968/1‑150 (MQ=255)

                                        ttATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGa                                                                                                               <  1:1993711/150‑1 (MQ=255)

                                         tATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAc                                                                                                              <  1:1571949/150‑1 (MQ=255)

                                         tATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAc                                                                                                              <  2:2543947/150‑1 (MQ=255)

                                           tGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCt                                                                                                            >  1:1481792/1‑150 (MQ=255)

                                              ttGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCACCTGCCGGTGATTATGTCGGTGACCTTtg                                                                                                         <  1:1689931/150‑1 (MQ=255)

                                                   gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                    <  2:4213749/150‑1 (MQ=255)

                                                   gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                    >  2:3313332/1‑150 (MQ=255)

                                                   gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                    >  2:455512/1‑150 (MQ=255)

                                                   gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                    >  1:3278289/1‑150 (MQ=255)

                                                       gACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTaa                                                                                                >  2:3636748/1‑150 (MQ=255)

                                                          tGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAacac                                                                                             >  2:890198/1‑150 (MQ=255)

                                                          tGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACAGTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAacac                                                                                             >  1:4429769/1‑150 (MQ=255)

                                                            ggCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCa                                                                                           <  2:1934568/150‑1 (MQ=255)

                                                                  tcAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATAGCAATGTATCGGTATCGGGAGCTTTAGGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATa                                                                                     >  2:2391350/1‑150 (MQ=255)

                                                                   cAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAg                                                                                    <  2:3293866/150‑1 (MQ=255)

                                                                      aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCa                                                                                 >  1:2224296/1‑150 (MQ=255)

                                                                      aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCa                                                                                 >  1:5075758/1‑150 (MQ=255)

                                                                        gaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAtt                                                                               <  1:3780165/150‑1 (MQ=255)

                                                                              aTTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATg                                                                         >  1:4679379/1‑150 (MQ=255)

                                                                              aTTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACAGTCTTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATg                                                                         >  1:3947410/1‑150 (MQ=255)

                                                                                   gATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                    >  1:10057/1‑150 (MQ=255)

                                                                                       cTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGat                                                                <  1:835449/150‑1 (MQ=255)

                                                                                        tCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCCTTGGGATGAACCCGGata                                                               >  1:1386194/1‑150 (MQ=255)

                                                                                        tCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGata                                                               >  1:3132464/1‑150 (MQ=255)

                                                                                        tCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGata                                                               <  1:3701441/150‑1 (MQ=255)

                                                                                        tCATTAGCTCGGATCTCTTGTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGata                                                               <  1:3882263/150‑1 (MQ=255)

                                                                                            tAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCgg                                                           >  1:4092606/1‑150 (MQ=255)

                                                                                              gCTCGGATCTCTTTTGCTGCGGGATCGTAACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGcc                                                         <  1:641868/150‑1 (MQ=255)

                                                                                               cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                        <  1:2121489/150‑1 (MQ=255)

                                                                                               cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                        <  2:2115511/150‑1 (MQ=255)

                                                                                               cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                        <  1:4964284/150‑1 (MQ=255)

                                                                                                tCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctg                                                       <  1:1734932/150‑1 (MQ=255)

                                                                                                 cGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgc                                                      >  2:3962285/1‑150 (MQ=255)

                                                                                                 cGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgc                                                      >  1:5262279/1‑150 (MQ=255)

                                                                                                  ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                     >  1:608616/1‑150 (MQ=255)

                                                                                                  ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTTCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                     <  2:3820898/150‑1 (MQ=255)

                                                                                                   gATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgctg                                                    >  2:2774060/1‑150 (MQ=255)

                                                                                                     tctcTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTggg                                                  >  2:5312579/1‑150 (MQ=255)

                                                                                                     tctcTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTggg                                                  >  2:1754707/1‑150 (MQ=255)

                                                                                                     tctcTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTggg                                                  >  1:2813200/1‑150 (MQ=255)

                                                                                                      ctctTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGAGAGCCATTGGGATGAACCCGGATATCgg                                                           >  2:3518845/1‑140 (MQ=255)

                                                                                                        ctTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGat                                               >  1:4480665/1‑150 (MQ=255)

                                                                                                            tgctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATAttt                                           <  2:143793/150‑1 (MQ=255)

                                                                                                            tgctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAAAGATAGCCAATGGGATGAACCCCGATAAAGGCCTGCTGGGGATAttt                                           >  2:5110722/1‑150 (MQ=255)

                                                                                                             gctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTg                                          <  2:471213/150‑1 (MQ=255)

                                                                                                              ctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTgg                                         >  2:2232888/1‑150 (MQ=255)

                                                                                                               tgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                        >  2:3207891/1‑150 (MQ=255)

                                                                                                               tgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                        <  1:3802956/150‑1 (MQ=255)

                                                                                                                 cGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGc                                      >  1:2314500/1‑150 (MQ=255)

                                                                                                                 cGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGc                                      >  2:593847/1‑150 (MQ=255)

                                                                                                                 cGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGc                                      >  2:3446719/1‑150 (MQ=255)

                                                                                                                  gggATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGcc                                     >  2:4093940/1‑150 (MQ=255)

                                                                                                                     aTCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACt                                  >  2:1125954/1‑150 (MQ=255)

                                                                                                                       cgccacaTTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTAt                                >  2:5550968/4‑150 (MQ=255)

                                                                                                                        gTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATTGGCCTGCTGGGGATATTTGGTGCCACTATc                               >  2:1843367/1‑150 (MQ=255)

                                                                                                                        gTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATTGGCCTGCTGGGGATATTTGGTGCCACTATc                               >  2:4674177/1‑150 (MQ=255)

                                                                                                                        gTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                               <  1:5485627/150‑1 (MQ=255)

                                                                                                                        gTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                               >  1:2567497/1‑150 (MQ=255)

                                                                                                                        gTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                               <  1:416620/150‑1 (MQ=255)

                                                                                                                           acaTTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgcc                            >  2:3580137/1‑150 (MQ=255)

                                                                                                                           acaTTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgcc                            <  1:425512/150‑1 (MQ=255)

                                                                                                                                  tGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtt                     >  1:4845152/1‑150 (MQ=255)

                                                                                                                                  tGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGAGATTTGGTGCCCCTATCGCCGCGGGtt                     >  1:25415/1‑150 (MQ=255)

                                                                                                                                  tGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGCATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtt                     <  1:792797/150‑1 (MQ=255)

                                                                                                                                    cAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtttt                   <  1:5394800/150‑1 (MQ=255)

                                                                                                                                    cAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtttt                   >  1:5266821/1‑150 (MQ=255)

                                                                                                                                      aTGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTAt                 >  2:1270119/1‑150 (MQ=255)

                                                                                                                                       tGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATc                >  2:2861608/1‑150 (MQ=255)

                                                                                                                                        gtatcggtatcgGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATca               <  2:10057/150‑1 (MQ=255)

                                                                                                                                         tatcggtatcggCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATcac              >  2:4309693/1‑150 (MQ=255)

                                                                                                                                         tatcggtatcggCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATcac              >  2:710616/1‑150 (MQ=255)

                                                                                                                                             ggtatcggCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACcaca          >  1:5252649/1‑150 (MQ=255)

                                                                                                                                               tatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatt        >  2:944415/1‑150 (MQ=255)

                                                                                                                                               tatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatt        <  2:3072986/150‑1 (MQ=255)

                                                                                                                                               tatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatt        <  1:324572/150‑1 (MQ=255)

                                                                                                                                                atcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatta       >  1:5531679/1‑150 (MQ=255)

                                                                                                                                                 tcggCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattat      <  1:932030/150‑1 (MQ=255)

                                                                                                                                                  cggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattatt     >  1:5010682/1‑150 (MQ=255)

                                                                                                                                                   ggCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACAgtatta    >  1:3426409/1‑150 (MQ=255)

CACGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGC  >  CP009273/3024834‑3025132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`