Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,076,526 C→A 63.3% D211Y (GAC→TAC)  speB ← agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,076,5260CA63.3% 272.7 / inf 338D211Y (GAC→TAC) speBagmatinase
Reads supporting (aligned to +/- strand):  ref base C (65/59);  new base A (97/117);  total (162/176)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 338 total aligned reads displayed.

GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCA  >  CP009273/3076379‑3076674
                                                                                                                                                   |                                                                                                                                                    
gTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCAt                                                                                                                                                    >  1:4475062/1‑150 (MQ=255)
 tACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATc                                                                                                                                                   >  1:2515828/1‑150 (MQ=255)
    cAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGATTCACTTGGGCGATAACGTCATCCAc                                                                                                                                                >  2:3793751/1‑150 (MQ=255)
    cAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCGGTTTCACTTGGGCGATAACGTAATCCAc                                                                                                                                                <  2:4273507/150‑1 (MQ=255)
     aGTTTAAGAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACg                                                                                                                                               <  2:1012756/150‑1 (MQ=255)
       tttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCt                                                                                                                                             <  2:1963933/150‑1 (MQ=255)
       tttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCt                                                                                                                                             <  2:2049622/150‑1 (MQ=255)
          aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCg                                                                                                                                          >  2:2288690/1‑150 (MQ=255)
          aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCg                                                                                                                                          >  1:4583085/1‑150 (MQ=255)
          aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACGGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCg                                                                                                                                          >  1:4741266/1‑150 (MQ=255)
             aGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATc                                                                                                                                       <  2:4815149/150‑1 (MQ=255)
                    tCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAcc                                                                                                                                >  2:1820308/1‑150 (MQ=255)
                     cGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACTAAATCCACGCTGCGATCGTTCACCt                                                                                                                               >  1:4836460/1‑150 (MQ=255)
                     cGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCt                                                                                                                               <  2:4656138/150‑1 (MQ=255)
                      ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTg                                                                                                                              <  2:53707/150‑1 (MQ=255)
                         ggTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCa                                                                                                                           <  2:2358530/150‑1 (MQ=255)
                          gTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAg                                                                                                                          >  1:4273243/1‑150 (MQ=255)
                           tCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAgg                                                                                                                         >  1:3834193/1‑150 (MQ=255)
                             aGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                       >  2:519715/1‑150 (MQ=255)
                                ccgccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTcc                                                                                                                    <  2:200416/150‑1 (MQ=255)
                                 cgccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCa                                                                                                                   >  2:454567/1‑150 (MQ=255)
                                           tGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTaaa                                                                                                         <  2:1678182/150‑1 (MQ=255)
                                            ggCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTGTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAg                                                                                                        >  2:1430631/1‑150 (MQ=255)
                                             gCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGc                                                                                                       <  1:3281109/150‑1 (MQ=255)
                                              cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGcc                                                                                                      >  1:272803/1‑150 (MQ=255)
                                              cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGcc                                                                                                      <  1:1498080/150‑1 (MQ=255)
                                               gTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCg                                                                                                     <  1:1672997/150‑1 (MQ=255)
                                                tACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGt                                                                                                    >  2:4378603/1‑150 (MQ=255)
                                                tACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGt                                                                                                    >  1:1007211/1‑150 (MQ=255)
                                                   cGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCCGGCAGGCGTCCAGCACGGTAAAGCCGTTGt                                                                                                 <  1:3244287/150‑1 (MQ=255)
                                                    ggTGCCTGGTGCAACAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTc                                                                                                <  2:2711016/150‑1 (MQ=255)
                                                       gCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCttt                                                                                             <  1:1002895/150‑1 (MQ=255)
                                                       gCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCttt                                                                                             <  1:3725105/150‑1 (MQ=255)
                                                          tGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                          >  1:2097504/1‑150 (MQ=255)
                                                           ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCa                                                                                         <  1:830583/150‑1 (MQ=255)
                                                             tGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCaaa                                                                                       >  2:1267403/1‑150 (MQ=255)
                                                              gCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAc                                                                                      <  1:4224984/150‑1 (MQ=255)
                                                              gCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAc                                                                                      <  1:635492/150‑1 (MQ=255)
                                                                 aaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCg                                                                                   >  2:1834958/1‑150 (MQ=255)
                                                                 aaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCg                                                                                   >  1:3855594/1‑150 (MQ=255)
                                                                 aaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATAACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCg                                                                                   >  1:1144577/1‑150 (MQ=255)
                                                                  aaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCgg                                                                                  >  1:3037440/1‑150 (MQ=255)
                                                                    gCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                                <  1:4384656/150‑1 (MQ=255)
                                                                      aGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACg                                                                              >  2:1471638/1‑150 (MQ=255)
                                                                      aGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACg                                                                              >  1:260809/1‑150 (MQ=255)
                                                                        gATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGaa                                                                            <  1:1840072/150‑1 (MQ=255)
                                                                        gATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGaa                                                                            <  1:3418679/150‑1 (MQ=255)
                                                                          tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATa                                                                          >  2:67101/1‑150 (MQ=255)
                                                                          tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAATCGGTACGAATa                                                                          >  1:5473143/1‑150 (MQ=255)
                                                                            cAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATAcc                                                                        >  2:5113762/1‑150 (MQ=255)
                                                                             aGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCa                                                                       <  1:5137656/150‑1 (MQ=255)
                                                                              ggCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCaa                                                                      >  1:2228498/1‑150 (MQ=255)
                                                                                cAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATc                                                                    >  1:1078997/1‑150 (MQ=255)
                                                                                cAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATc                                                                    <  1:719053/150‑1 (MQ=255)
                                                                                cAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATc                                                                    >  2:2992089/1‑150 (MQ=255)
                                                                                       tatCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTg                                                                                                  >  1:12816/1‑113 (MQ=255)
                                                                                        atCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGa                                                            <  1:5559813/150‑1 (MQ=255)
                                                                                         tCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGaa                                                           >  1:3503885/1‑150 (MQ=255)
                                                                                          cAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAAt                                                          <  2:4171745/150‑1 (MQ=255)
                                                                                             aaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAt                                                       <  2:2867516/150‑1 (MQ=255)
                                                                                               gTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTc                                                     <  1:4519594/150‑1 (MQ=255)
                                                                                                tCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCg                                                    <  1:589142/150‑1 (MQ=255)
                                                                                                tCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCg                                                    <  1:225929/150‑1 (MQ=255)
                                                                                                    gTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTc                                                <  2:3352977/150‑1 (MQ=255)
                                                                                                      aaaCCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGa                                              <  2:2124945/150‑1 (MQ=255)
                                                                                                          cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                          >  2:3914536/1‑150 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGa                                         >  1:4408135/1‑150 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGa                                         <  1:2484493/150‑1 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAAAGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGTAATGATACGGGTCTATCAGa                                         >  1:2869572/1‑150 (MQ=255)
                                                                                                             cATATCACCCTCAATCTGTTTCACTTGGGCGATAACGTCTGCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACTAATACCAATCTGCACGGAATGATTCGGGTCGTTCAGAcc                                       <  2:1481370/150‑1 (MQ=255)
                                                                                                              atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                      <  1:4815359/150‑1 (MQ=255)
                                                                                                              atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                      >  2:2743599/1‑150 (MQ=255)
                                                                                                              atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                      <  1:3025374/150‑1 (MQ=255)
                                                                                                              atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCATTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                      >  1:2030378/1‑150 (MQ=255)
                                                                                                               tatCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACctt                                     >  2:3596221/1‑150 (MQ=255)
                                                                                                                   aCCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCttt                                 >  2:1081415/1‑150 (MQ=255)
                                                                                                                    cccACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTc                                <  2:507395/150‑1 (MQ=255)
                                                                                                                     ccACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                               >  2:2624272/1‑150 (MQ=255)
                                                                                                                     ccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                               >  1:3202266/1‑150 (MQ=255)
                                                                                                                         aaTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGgcgc                           <  1:361187/150‑1 (MQ=255)
                                                                                                                          aTCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGcgcg                          >  2:1542732/1‑150 (MQ=255)
                                                                                                                          aTCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGcgcg                          >  2:5587920/1‑150 (MQ=255)
                                                                                                                            cTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGt                        <  1:4014750/150‑1 (MQ=255)
                                                                                                                            cTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGt                        <  2:5458553/150‑1 (MQ=255)
                                                                                                                              gTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGtat                      >  1:1809812/1‑150 (MQ=255)
                                                                                                                                ttCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAg                    <  1:1989801/150‑1 (MQ=255)
                                                                                                                                 tCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGa                   <  1:138220/150‑1 (MQ=255)
                                                                                                                                  cACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGaa                  <  1:4685933/150‑1 (MQ=255)
                                                                                                                                    cTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACa                <  1:3403824/150‑1 (MQ=255)
                                                                                                                                    cTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACa                >  2:1642004/1‑150 (MQ=255)
                                                                                                                                     ttGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAt               >  2:1306363/1‑150 (MQ=255)
                                                                                                                                      tGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa              >  1:411972/1‑150 (MQ=255)
                                                                                                                                      tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa              <  1:3117109/150‑1 (MQ=255)
                                                                                                                                      tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa              >  2:4716451/1‑150 (MQ=255)
                                                                                                                                         gCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTg           >  2:90877/1‑150 (MQ=255)
                                                                                                                                          cGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGc          >  1:1763571/1‑150 (MQ=255)
                                                                                                                                             tAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGt       >  2:760933/1‑150 (MQ=255)
                                                                                                                                              aaCGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTg      <  2:738990/150‑1 (MQ=255)
                                                                                                                                                cGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGt    >  2:4764200/1‑150 (MQ=255)
                                                                                                                                                 gTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTc   >  2:3165946/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCA  >  CP009273/3076379‑3076674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑