Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,346,662 G→T 5.4% R250S (CGC→AGC)  yhcC ← putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,346,6620GT 5.4% 1232.2 / 30.2 320R250S (CGC→AGC) yhcCputative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (126/176);  new base T (5/13);  total (131/189)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

CGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCA  >  CP009273/3346513‑3346811
                                                                                                                                                     |                                                                                                                                                     
 gcCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGa                                                                                                                                                      >  1:5177684/1‑150 (MQ=255)
   cGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAAt                                                                                                                                                    >  2:438984/1‑150 (MQ=255)
   cGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAAt                                                                                                                                                    >  1:499231/1‑150 (MQ=255)
    gATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATc                                                                                                                                                   >  2:1825987/1‑150 (MQ=255)
    gATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATc                                                                                                                                                   >  1:2683827/1‑150 (MQ=255)
     aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCa                                                                                                                                                  >  2:1220172/1‑150 (MQ=255)
        ccTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAttt                                                                                                                                               <  1:1127199/150‑1 (MQ=255)
        ccTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAttt                                                                                                                                               >  1:4742952/1‑150 (MQ=255)
        ccTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTGTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAttt                                                                                                                                               <  1:1020268/150‑1 (MQ=255)
         cTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTtc                                                                                                                                              <  1:1599891/150‑1 (MQ=255)
            tACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCTAATCATTTCTcc                                                                                                                                           >  2:5070032/1‑150 (MQ=255)
                 cATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCTGCAg                                                                                                                                      >  1:1564941/1‑150 (MQ=255)
                  aTGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGt                                                                                                                                     >  2:4553376/1‑150 (MQ=255)
                     ttcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAg                                                                                                                                  <  2:3967000/150‑1 (MQ=255)
                     ttcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAg                                                                                                                                  <  2:1124682/150‑1 (MQ=255)
                      tcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGc                                                                                                                                 >  1:2632471/1‑150 (MQ=255)
                        attcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCgt                                                                                                                               >  1:5350196/1‑150 (MQ=255)
                            aGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTaa                                                                                                                           <  1:581271/150‑1 (MQ=255)
                              aTAGCGATCCATCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGTGCAAGCAGCGTCGTGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCTAATCATTTCTCCGGCAGTGAGCGTGTAAtc                                                                                                                         <  1:5006042/150‑1 (MQ=255)
                              aTAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtc                                                                                                                         >  2:5310480/1‑150 (MQ=255)
                              aTAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtc                                                                                                                         <  1:349825/150‑1 (MQ=255)
                               tAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcc                                                                                                                        <  2:4557804/150‑1 (MQ=255)
                                aGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCTAATCATTTCTCCGGCAGTGAGCGTGTAAtcct                                                                                                                       <  2:242257/150‑1 (MQ=255)
                                    aTCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAg                                                                                                                   <  1:5238472/150‑1 (MQ=255)
                                     tCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGt                                                                                                                  >  2:3294942/1‑150 (MQ=255)
                                       cAGCTCGACCAGTCCCGTCCATCGATTTTCGCACCACAGCGGAGCACGCAGCGTCGGGCGACGGGCGCTGGCGGACCTGCGGTGGTAGATCACTTCCGGTGGCGGATGGCGAATCATTTCTCCGGCAGTGAGCGTGGAATCCTCCAGTTc                                                                                                                >  2:2674027/1‑150 (MQ=255)
                                           tCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGTGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATa                                                                                                            >  1:14078/1‑150 (MQ=255)
                                             gACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATAcc                                                                                                          >  1:4595957/1‑150 (MQ=255)
                                              aCCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCg                                                                                                         <  1:2925666/150‑1 (MQ=255)
                                              aCCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCg                                                                                                         <  1:4457305/150‑1 (MQ=255)
                                                cctgcccGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGtt                                                                                                       <  2:4282946/146‑1 (MQ=255)
                                                cATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGtt                                                                                                       >  2:295735/1‑150 (MQ=255)
                                                  ttCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCa                                                                                                     <  1:2425576/150‑1 (MQ=255)
                                                   tCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCaa                                                                                                    <  2:2182202/150‑1 (MQ=255)
                                                      cGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACg                                                                                                 >  2:1892479/1‑150 (MQ=255)
                                                         ccATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGccc                                                                                              <  2:1480254/150‑1 (MQ=255)
                                                             cGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCt                                                                                          <  2:5116013/150‑1 (MQ=255)
                                                              gATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCTAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCtt                                                                                         <  2:4719720/150‑1 (MQ=255)
                                                              gATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCtt                                                                                         >  2:1754750/1‑150 (MQ=255)
                                                              gATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCtt                                                                                         >  2:4465518/1‑150 (MQ=255)
                                                               aTTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTTTGGCGAATCATTTCTCCGGCAGTGAGCGAGTACTCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTc                                                                                        >  2:2992081/1‑150 (MQ=255)
                                                               aTTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTc                                                                                        <  2:4134078/150‑1 (MQ=255)
                                                                 tttCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTccc                                                                                      <  2:223291/150‑1 (MQ=255)
                                                                        ccacAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCttt                                                                               <  1:4966275/150‑1 (MQ=255)
                                                                        ccacAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCttt                                                                               <  2:730804/150‑1 (MQ=255)
                                                                        ccacAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCttt                                                                               <  1:5328321/150‑1 (MQ=255)
                                                                         cacaGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCTAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTc                                                                              <  2:3636372/150‑1 (MQ=255)
                                                                            aGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGcc                                                                           >  1:2345665/1‑150 (MQ=255)
                                                                            aGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGcc                                                                           <  1:4111531/150‑1 (MQ=255)
                                                                             gCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCa                                                                          <  2:5352177/150‑1 (MQ=255)
                                                                             gCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCa                                                                          <  2:2004255/150‑1 (MQ=255)
                                                                              cGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCAt                                                                         <  2:3102631/150‑1 (MQ=255)
                                                                               ggcgcaagcaGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATa                                                                        <  1:3417697/147‑1 (MQ=255)
                                                                                gAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCTAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATaa                                                                       <  1:2997492/150‑1 (MQ=255)
                                                                                    aagcaGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAAtgct                                                                   <  1:577101/150‑1 (MQ=255)
                                                                                          cGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCttt                                                             <  2:334568/150‑1 (MQ=255)
                                                                                           gTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTc                                                            <  1:1057407/150‑1 (MQ=255)
                                                                                             cGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTcac                                                          >  2:1404350/1‑150 (MQ=255)
                                                                                              gggCGACGGGCGCGGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTcaca                                                         <  2:4442270/150‑1 (MQ=255)
                                                                                               ggCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACaa                                                        <  1:405168/150‑1 (MQ=255)
                                                                                                  gACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAatat                                                     <  2:1621980/150‑1 (MQ=255)
                                                                                                  gACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAatat                                                     >  1:5567204/1‑150 (MQ=255)
                                                                                                    cGGGCGCTGGCGGAAATTCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGc                                                   <  2:4674799/150‑1 (MQ=255)
                                                                                                    cGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATCATGCTGCCTTTCACAATATGc                                                   >  2:906659/1‑150 (MQ=255)
                                                                                                      ggCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAg                                                 <  2:2757333/150‑1 (MQ=255)
                                                                                                       gcgcTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGc                                                <  2:2805596/150‑1 (MQ=255)
                                                                                                          cTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGa                                             <  2:5023482/150‑1 (MQ=255)
                                                                                                              cGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCa                                         <  1:619217/150‑1 (MQ=255)
                                                                                                              cGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCa                                         >  2:612147/1‑150 (MQ=255)
                                                                                                               ggAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAg                                        <  1:977071/150‑1 (MQ=255)
                                                                                                               ggAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAg                                        <  1:1825987/150‑1 (MQ=255)
                                                                                                                   aTGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCttt                                    <  2:1676883/150‑1 (MQ=255)
                                                                                                                   aTGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCttt                                    <  2:1178478/150‑1 (MQ=255)
                                                                                                                    tGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTa                                   >  1:2779601/1‑150 (MQ=255)
                                                                                                                    tGCGGTGGTAGATCACTTCCGGGGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTAAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTa                                   <  1:1683689/150‑1 (MQ=255)
                                                                                                                     gCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTAt                                  <  2:5516720/150‑1 (MQ=255)
                                                                                                                     gCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTAt                                  <  1:2588881/150‑1 (MQ=255)
                                                                                                                     gCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTAt                                  <  2:639762/150‑1 (MQ=255)
                                                                                                                      cGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATg                                 <  1:4752324/150‑1 (MQ=255)
                                                                                                                      cGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATg                                 <  2:824738/150‑1 (MQ=255)
                                                                                                                              gATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCAc                         <  2:4877558/150‑1 (MQ=255)
                                                                                                                               aTCACGTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACg                        <  2:948044/150‑1 (MQ=255)
                                                                                                                                 cACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGcc                      <  1:2145543/150‑1 (MQ=255)
                                                                                                                                    ttCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGt                   <  1:2042745/150‑1 (MQ=255)
                                                                                                                                     tCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGtt                  <  2:2095874/150‑1 (MQ=255)
                                                                                                                                       cGGTTGCGTATGGCGAATCATTTCTCCGGCAGTTAGCGTGTAATCCTCCAGTTCAATACAGTTCACACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCATCGGATGCAGCTTTATGCCGTCCACGCCGGTTTc                <  1:2203627/150‑1 (MQ=255)
                                                                                                                                         gTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCaa              <  2:1404169/150‑1 (MQ=255)
                                                                                                                                          tGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAAc             <  1:852457/150‑1 (MQ=255)
                                                                                                                                          tGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAAc             <  1:4255087/150‑1 (MQ=255)
                                                                                                                                           ggCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAAcc            <  2:5572478/150‑1 (MQ=255)
                                                                                                                                             cGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCAc          <  1:111455/150‑1 (MQ=255)
                                                                                                                                               tATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTc        >  2:3849251/1‑150 (MQ=255)
                                                                                                                                                aTGGCGAATCATTTCTCCGGCAGTTAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCg       >  1:3909201/1‑150 (MQ=255)
                                                                                                                                                aTGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCg       >  1:2511667/1‑150 (MQ=255)
                                                                                                                                                  ggCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCt     <  1:3158689/150‑1 (MQ=255)
                                                                                                                                                  ggCGAATCATTTCACCGGCAGTGAGCGTGGAATCCTCCAGTTCAAGACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCt     <  1:4267289/150‑1 (MQ=255)
                                                                                                                                                    cGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCCCTCGCTcc   >  1:2175222/1‑150 (MQ=255)
                                                                                                                                                    cGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTcc   <  2:1538877/150‑1 (MQ=255)
                                                                                                                                                    cGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTcc   <  2:2030908/150‑1 (MQ=255)
                                                                                                                                                     gAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCa  >  2:2497443/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCTTCCCAGGCTTTCGCCATAATGCTGCCTTTCACAATATGCAGCGGATGCAGCTTTATGCCGTCCACGCCGGTTTCAACCACTCGCTCCA  >  CP009273/3346513‑3346811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑