Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,471,787 C→A 11.7% D24Y (GAT→TAT)  slyD ← FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,471,7870CA11.7% 1104.6 / 87.9 324D24Y (GAT→TAT) slyDFKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (137/149);  new base A (12/26);  total (149/175)
Fisher's exact test for biased strand distribution p-value = 8.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 346 total aligned reads displayed.

TCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAA  >  CP009273/3471639‑3471936
                                                                                                                                                    |                                                                                                                                                     
     gtACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaaccaa                                                                                                                                                 >  2:5281428/1‑150 (MQ=255)
      tACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaaccaac                                                                                                                                                <  1:2100008/150‑1 (MQ=255)
        cTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAacac                                                                                                                                              >  2:5074189/1‑150 (MQ=255)
         tGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAacaca                                                                                                                                             >  1:109595/1‑150 (MQ=255)
           aCCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACAcc                                                                                                                                           <  1:4097306/150‑1 (MQ=255)
             cGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGt                                                                                                                                         >  2:1777760/1‑150 (MQ=255)
              gTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtc                                                                                                                                        >  2:3767950/1‑150 (MQ=255)
                aaGCGTCGTTCGCGCCCACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTGTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTCGTCCAGCGGCGCACTCACCGGAGCCGCATCCACCAACACACCGtctt                                                                                                                                      >  2:1328603/1‑150 (MQ=255)
                aaGCGTCGGTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCGTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtctt                                                                                                                                      <  2:4868468/150‑1 (MQ=255)
                 aGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGtcttc                                                                                                                                     <  1:4897316/150‑1 (MQ=255)
                 aGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGtcttc                                                                                                                                     <  1:1317851/150‑1 (MQ=255)
                 aGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCAGGACCTTCCCGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttc                                                                                                                                     <  2:1651386/150‑1 (MQ=255)
                  gCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttct                                                                                                                                    >  1:5441593/1‑150 (MQ=255)
                  gCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttct                                                                                                                                    <  2:4691445/150‑1 (MQ=255)
                  gCGTCGTTCGCGCCAACAGCGACATCAAATGTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttct                                                                                                                                    <  2:2131308/150‑1 (MQ=255)
                       gtTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgtacg                                                                                                                               <  2:1090238/150‑1 (MQ=255)
                          cgcgCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgtacgtac                                                                                                                            <  1:3727962/150‑1 (MQ=255)
                           gcgcCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTAcc                                                                                                                           <  2:5002652/150‑1 (MQ=255)
                             gcCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTg                                                                                                                         >  1:1024592/1‑150 (MQ=255)
                              ccAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGa                                                                                                                        >  2:1573186/1‑150 (MQ=255)
                               cAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGAt                                                                                                                       >  2:4463360/1‑150 (MQ=255)
                               cAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGAt                                                                                                                       <  1:118472/150‑1 (MQ=255)
                               cAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGAt                                                                                                                       >  1:3505445/1‑150 (MQ=255)
                                aaCAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATa                                                                                                                      >  1:238452/1‑150 (MQ=255)
                                 aCAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAg                                                                                                                     >  1:2464274/1‑150 (MQ=255)
                                 aCAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAg                                                                                                                     <  2:3212513/150‑1 (MQ=255)
                                   aGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGc                                                                                                                   <  2:2031352/150‑1 (MQ=255)
                                   aGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGc                                                                                                                   >  1:585281/1‑150 (MQ=255)
                                   aGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGc                                                                                                                   >  1:771363/1‑150 (MQ=255)
                                     cGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCa                                                                                                                 <  2:5599007/150‑1 (MQ=255)
                                      gACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAg                                                                                                                >  1:1950527/1‑150 (MQ=255)
                                      gACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAg                                                                                                                <  1:1251813/150‑1 (MQ=255)
                                      gACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAg                                                                                                                >  2:1053485/1‑150 (MQ=255)
                                         aTCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCt                                                                                                             >  1:3160786/1‑150 (MQ=255)
                                         aTCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCt                                                                                                             >  1:4994292/1‑150 (MQ=255)
                                          tCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTg                                                                                                            <  2:705462/150‑1 (MQ=255)
                                              aTTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGacca                                                                                                        >  1:1015522/1‑150 (MQ=255)
                                               tttGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGaccac                                                                                                       >  2:621186/1‑150 (MQ=255)
                                                 tGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGAccacca                                                                                                     <  2:2719537/150‑1 (MQ=255)
                                                 tGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGAccacca                                                                                                     <  2:3598288/150‑1 (MQ=255)
                                                 tGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGAccacca                                                                                                     <  2:5527845/150‑1 (MQ=255)
                                                        aaCTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCttt                                                                                              <  2:2073412/150‑1 (MQ=255)
                                                        aaCTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCttt                                                                                              <  2:853866/150‑1 (MQ=255)
                                                          cTTCATGACCTTCCCGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGACTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTGTTg                                                                                            >  2:4517579/1‑150 (MQ=255)
                                                            tCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCt                                                                                          >  1:4657780/1‑150 (MQ=255)
                                                             cATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTa                                                                                         <  1:3015377/150‑1 (MQ=255)
                                                               tcaCCATCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACt                                                                                       <  2:3030732/148‑1 (MQ=255)
                                                                gACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCGGACCACCAGGTCTTTTGCTACtt                                                                                      <  1:2516293/150‑1 (MQ=255)
                                                                  ccTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTc                                                                                    <  2:5541204/150‑1 (MQ=255)
                                                                    ttCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCag                                                                                  >  1:3427037/1‑149 (MQ=255)
                                                                    ttCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCAt                                                                                  <  1:666110/150‑1 (MQ=255)
                                                                     tCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATg                                                                                 >  1:5303660/1‑150 (MQ=255)
                                                                       cAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGat                                                                               <  1:4769384/150‑1 (MQ=255)
                                                                       cAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGat                                                                               >  1:5215683/1‑150 (MQ=255)
                                                                        aGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGata                                                                              >  2:1964747/1‑150 (MQ=255)
                                                                         gcgcCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGatat                                                                             >  2:4129562/1‑150 (MQ=255)
                                                                          cgcCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATAtc                                                                            >  2:367616/1‑150 (MQ=255)
                                                                           gcCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGatatat                                                                           <  1:1688390/150‑3 (MQ=255)
                                                                           gcCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATAtct                                                                           <  2:4920693/150‑1 (MQ=255)
                                                                             cctttCCCGGCCAGAGCTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTcc                                                                         <  2:5223907/148‑1 (MQ=255)
                                                                              gcttCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCt                                                                        <  2:1896507/148‑1 (MQ=255)
                                                                                ttCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGa                                                                      <  1:1908263/150‑1 (MQ=255)
                                                                                   cAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCa                                                                   <  2:2921513/150‑1 (MQ=255)
                                                                                   cAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCa                                                                   <  2:311976/150‑1 (MQ=255)
                                                                                        cAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGga                                                              <  2:1912710/150‑1 (MQ=255)
                                                                                         agagATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaa                                                             <  2:2491026/150‑1 (MQ=255)
                                                                                            gaTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaagaa                                                          <  1:203101/150‑1 (MQ=255)
                                                                                            gaTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaagaa                                                          <  1:203752/150‑1 (MQ=255)
                                                                                            gaTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaagaa                                                          <  1:3922230/150‑1 (MQ=255)
                                                                                            gaTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaagaa                                                          <  1:1079531/150‑1 (MQ=255)
                                                                                            gaTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGgaagaa                                                          >  1:2759470/1‑150 (MQ=255)
                                                                                             aTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAAt                                                         >  1:1500512/1‑150 (MQ=255)
                                                                                             aTCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAAt                                                         <  1:4272542/150‑1 (MQ=255)
                                                                                               cAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAg                                                       >  2:2591076/1‑150 (MQ=255)
                                                                                               cAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAg                                                       <  1:3840042/150‑1 (MQ=255)
                                                                                                    aaCCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGgcg                                                  <  2:5334325/150‑1 (MQ=255)
                                                                                                    aaCCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGgcg                                                  >  2:1708665/1‑150 (MQ=255)
                                                                                                           aCCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTg                                           >  1:2235109/1‑150 (MQ=255)
                                                                                                            ccATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGt                                          <  1:333417/150‑1 (MQ=255)
                                                                                                             cATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTa                                         >  2:4376022/1‑150 (MQ=255)
                                                                                                               tGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGc                                       <  2:4216189/150‑1 (MQ=255)
                                                                                                                 cAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCgg                                     >  1:548897/1‑150 (MQ=255)
                                                                                                                  aGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATATTGGCGCAGATTGTAGCGGa                                    >  2:3015224/1‑150 (MQ=255)
                                                                                                                    gTAGTCCAGCGGCGCACTCCCCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAAt                                  <  2:3940277/150‑1 (MQ=255)
                                                                                                                    gTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAAt                                  <  1:819519/150‑1 (MQ=255)
                                                                                                                    gTAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAAt                                  <  1:2938968/150‑1 (MQ=255)
                                                                                                                     tAGTCCAGCGGCGCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAAtt                                 <  1:5260246/150‑1 (MQ=255)
                                                                                                                       gTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCt                               <  1:3394491/150‑1 (MQ=255)
                                                                                                                           aGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAg                           <  1:4403471/150‑1 (MQ=255)
                                                                                                                             cggcgCACTCACCGGAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGcc                         <  2:771363/150‑1 (MQ=255)
                                                                                                                                cgcACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCgtg                      >  2:1470980/1‑150 (MQ=255)
                                                                                                                                cgcACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCgtg                      >  2:1394349/1‑150 (MQ=255)
                                                                                                                                  cACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTa                    <  2:3339176/150‑1 (MQ=255)
                                                                                                                                     tCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTc                 <  2:2354087/150‑1 (MQ=255)
                                                                                                                                      cACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCa                >  1:3239276/1‑150 (MQ=255)
                                                                                                                                          ggAGACTCATAAACCACCACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAg            <  2:3750065/150‑1 (MQ=255)
                                                                                                                                          ggAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAg            <  2:3750577/150‑1 (MQ=255)
                                                                                                                                          ggAGACTCATAAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAg            >  2:842420/1‑150 (MQ=255)
                                                                                                                                             gaCTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCtt         <  2:352873/150‑1 (MQ=255)
                                                                                                                                                    caaccaacACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTaaaaaaa  >  2:474032/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAA  >  CP009273/3471639‑3471936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑