Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,636,207 T→A 12.7% T96S (ACT→TCT)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,636,2070TA12.7% 1148.6 / 133.8 376T96S (ACT→TCT) rsmJ16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (176/151);  new base A (23/25);  total (199/177)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 380 total aligned reads displayed.

CTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCACGCT  >  CP009273/3636058‑3636356
                                                                                                                                                     |                                                                                                                                                     
cTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGt                                                                                                                                                       <  1:1847447/150‑1 (MQ=255)
   cTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGc                                                                                                                                                    <  1:457510/150‑1 (MQ=255)
   cTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGc                                                                                                                                                    >  2:1416928/1‑150 (MQ=255)
     gCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCAt                                                                                                                                                  >  2:3953149/1‑150 (MQ=255)
       aaCCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATcc                                                                                                                                                >  2:4369929/1‑150 (MQ=255)
       aaCCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATcc                                                                                                                                                >  2:1534896/1‑150 (MQ=255)
       aaCCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCCTCCAGACGCGCGGCCACCACTCGATAACGCTCCAGCATCCGCACGCGCCAGCCGACAGAAGCCAGACCAAAGGCATCGCGCCCAAGTCCGCCACTGGCATcc                                                                                                                                                >  2:2100480/1‑150 (MQ=255)
         ccAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccac                                                                                                                                              >  2:3368892/1‑150 (MQ=255)
         ccAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccac                                                                                                                                              >  2:1744121/1‑150 (MQ=255)
         ccAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATccac                                                                                                                                              >  2:37037/1‑150 (MQ=255)
              cgccgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACAt                                                                                                                                         >  2:5417305/1‑150 (MQ=255)
               gccgATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATc                                                                                                                                        <  2:1717341/150‑1 (MQ=255)
                  naTTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCgg                                                                                                                                     >  2:5334885/2‑150 (MQ=255)
                    tttCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCa                                                                                                                                   >  1:4924180/1‑150 (MQ=255)
                    tttCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCa                                                                                                                                   >  1:2712401/1‑150 (MQ=255)
                        cgcatccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAaata                                                                                                                               >  2:4053770/1‑150 (MQ=255)
                           atccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATc                                                                                                                            >  1:1171847/1‑150 (MQ=255)
                           atccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATc                                                                                                                            <  1:4430503/150‑1 (MQ=255)
                             ccgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGc                                                                                                                          <  1:695879/150‑1 (MQ=255)
                              cgcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGcc                                                                                                                         <  2:1834392/150‑1 (MQ=255)
                               gcatAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCt                                                                                                                        >  1:3235788/1‑150 (MQ=255)
                                  tAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTa                                                                                                                     <  2:3917953/150‑1 (MQ=255)
                                   aaCCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTaa                                                                                                                    <  2:1980912/150‑1 (MQ=255)
                                         gcgcCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCa                                                                                                              <  1:1364693/150‑1 (MQ=255)
                                         gcgcCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCa                                                                                                              <  1:3884078/150‑1 (MQ=255)
                                          cgcCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCAc                                                                                                             <  2:2203121/150‑1 (MQ=255)
                                          cgcCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCAc                                                                                                             >  1:2050362/1‑150 (MQ=255)
                                           gcCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCAcc                                                                                                            <  2:3417743/150‑1 (MQ=255)
                                            ccAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCg                                                                                                           <  1:1472835/150‑1 (MQ=255)
                                                gCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCttt                                                                                                       <  1:1695584/150‑1 (MQ=255)
                                                gCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCttt                                                                                                       >  2:4225056/1‑150 (MQ=255)
                                                 ccGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTc                                                                                                      >  2:2786023/1‑150 (MQ=255)
                                                  cgtcgtCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCg                                                                                                     <  2:5529740/150‑1 (MQ=255)
                                                   gtcgtcGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCACATAATCGCCTTTAATGCCCACCGCTGTCGc                                                                                                    >  1:4049765/1‑150 (MQ=255)
                                                    tcgtcgAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGcc                                                                                                   >  1:4732360/1‑150 (MQ=255)
                                                         gAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGc                                                                                              >  2:2299179/1‑150 (MQ=255)
                                                            tAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTc                                                                                           <  2:1773483/150‑1 (MQ=255)
                                                            tAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTc                                                                                           >  2:4172738/1‑150 (MQ=255)
                                                             aGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCa                                                                                          <  1:1824378/150‑1 (MQ=255)
                                                             aGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCa                                                                                          <  2:4989764/150‑1 (MQ=255)
                                                                  ggCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACCTCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACcgcg                                                                                     >  2:2589969/1‑150 (MQ=255)
                                                                   gCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGa                                                                                    <  1:2541090/150‑1 (MQ=255)
                                                                    cAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTCATGCCCACCGCTTTCGCCACCGCCTCACCGCGac                                                                                   >  2:2840461/1‑150 (MQ=255)
                                                                    cAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGac                                                                                   >  2:4950462/1‑150 (MQ=255)
                                                                    cAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGac                                                                                   >  2:3591643/1‑150 (MQ=255)
                                                                        cACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGaccacc                                                                               <  1:1747291/150‑1 (MQ=255)
                                                                           ggcaTTCCGCCCCAGCCCCCCCACGCCGCAGCCCACTGCAGCCACCACCCCGCCCTCGCGCCCAAGCCCCGCCGACCCAACCCCCACCCCCGGCACCTCAGCGCCATTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCAccgcc                                                                            <  2:3970608/147‑1 (MQ=255)
                                                                             gATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGa                                                                          <  2:1208519/150‑1 (MQ=255)
                                                                               ttACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAAt                                                                        <  1:2002217/150‑1 (MQ=255)
                                                                                   gCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGc                                                                    >  1:864283/1‑150 (MQ=255)
                                                                                      ccAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTc                                                                 <  1:4543324/150‑1 (MQ=255)
                                                                                        aGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCgg                                                               >  2:2708896/1‑150 (MQ=255)
                                                                                         gCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGt                                                              <  1:4920954/150‑1 (MQ=255)
                                                                                            tCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTgcg                                                           >  2:4021371/1‑150 (MQ=255)
                                                                                            tCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTgcg                                                           >  1:4007747/1‑150 (MQ=255)
                                                                                             ccGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTgcgc                                                          >  1:288185/1‑150 (MQ=255)
                                                                                              cGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGcc                                                         <  2:4806414/150‑1 (MQ=255)
                                                                                               gCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCa                                                        >  2:4504548/1‑150 (MQ=255)
                                                                                                     ggCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTc                                                  >  1:5552366/1‑150 (MQ=255)
                                                                                                       cAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCg                                                <  1:4759523/150‑1 (MQ=255)
                                                                                                       cAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCg                                                <  1:333886/150‑1 (MQ=255)
                                                                                                         gCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGcc                                              <  2:3321659/150‑1 (MQ=255)
                                                                                                          ccGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGAATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCca                                             <  1:2409019/150‑1 (MQ=255)
                                                                                                           cGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCcaa                                            >  1:3803803/1‑150 (MQ=255)
                                                                                                            gACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCcaac                                           <  1:3558570/150‑1 (MQ=255)
                                                                                                             aCTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCcaaca                                          <  2:2972425/150‑1 (MQ=255)
                                                                                                               tGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACaaa                                        >  2:5232914/1‑150 (MQ=255)
                                                                                                                 aaGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAAt                                      >  2:307572/1‑150 (MQ=255)
                                                                                                                   gCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATca                                    >  1:989198/1‑150 (MQ=255)
                                                                                                                    ccAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaa                                   >  1:4892995/1‑150 (MQ=255)
                                                                                                                     cAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaac                                  <  2:1902840/150‑1 (MQ=255)
                                                                                                                      aGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaaca                                 <  1:4369742/150‑1 (MQ=255)
                                                                                                                       gTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaacaa                                <  2:1631183/150‑1 (MQ=255)
                                                                                                                       gTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaacaa                                >  1:5389860/1‑150 (MQ=255)
                                                                                                                       gTACAAAGGCATCGCGCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATcaacaa                                >  1:550098/1‑150 (MQ=255)
                                                                                                                         aCAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAAACGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCAATCGCTCCGCCAACAAAATCAACAAAg                              >  1:5141752/1‑150 (MQ=255)
                                                                                                                            aaGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATg                           <  1:4053770/150‑1 (MQ=255)
                                                                                                                               gCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgccg                        <  1:2633544/150‑1 (MQ=255)
                                                                                                                                 aTCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCATTAATGCCCACCGCTTTCGCCACCGACTCACCGCGACCACCGCCGAATTTGAGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATgccgcc                      >  1:3459274/1‑150 (MQ=255)
                                                                                                                                   cgcgCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCaa                    <  2:1112985/150‑1 (MQ=255)
                                                                                                                                   cgcgCCCAAGTCCTGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCaa                    <  2:3226639/150‑1 (MQ=255)
                                                                                                                                    gcgcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAg                   >  2:1942509/1‑150 (MQ=255)
                                                                                                                                     cgcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGt                  >  2:5643268/1‑150 (MQ=255)
                                                                                                                                      gcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGtt                 <  2:5507930/150‑1 (MQ=255)
                                                                                                                                      gcCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGtt                 <  2:5103919/150‑1 (MQ=255)
                                                                                                                                       cccAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGttt                <  1:37037/150‑1 (MQ=255)
                                                                                                                                        ccAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGtttt               >  2:2289037/1‑150 (MQ=255)
                                                                                                                                        ccAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGtttt               >  2:4705390/1‑150 (MQ=255)
                                                                                                                                         cAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTg              <  2:2408935/150‑1 (MQ=255)
                                                                                                                                          aaGTCCTGCAGTGGCATCCACCACATCCGGCACATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGAGCGCCATCGCTACGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTgg             >  2:1160980/1‑150 (MQ=255)
                                                                                                                                          aaGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTgg             >  2:1160673/1‑150 (MQ=255)
                                                                                                                                          aaGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTgg             >  1:2165003/1‑150 (MQ=255)
                                                                                                                                            gTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCt           <  1:2794396/150‑1 (MQ=255)
                                                                                                                                             tCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtc          >  2:5582959/1‑150 (MQ=255)
                                                                                                                                             tCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtc          <  1:1077383/150‑1 (MQ=255)
                                                                                                                                             tCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtc          <  2:839370/150‑1 (MQ=255)
                                                                                                                                               cTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtcat        <  1:2968350/150‑1 (MQ=255)
                                                                                                                                                tGCAGAGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCtcatc       <  2:4908105/150‑1 (MQ=255)
                                                                                                                                                   aGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCACg    >  2:695769/1‑150 (MQ=255)
                                                                                                                                                     tGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCTTCACGCt  >  2:4269243/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCCTGCAACCAGCCGCCGATTTCCGCATCCGCATAACCACGCGCCAGGCCGTCGTCGAGTAGCGCGGCAACCACTGGATTACGCTCCAGCATCCGCACGCGGCAGCCGACTGAAGCCAGTACAAAGGCATCGCGCCCAAGTCCTGCAGTGGCATCCACCACATCCGGCAAATAATCGCCTTTAATGCCCACCGCTTTCGCCACCGCCTCACCGCGACCACCGCCGAATTTGCGTCGGTGCGCCATCGCTCCGCCAACAAAATCAACAAAGATGCCGCCAAGTTTTGGCTCATCACGCT  >  CP009273/3636058‑3636356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑