Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,675,614 A→T 5.6% V398V (GTT→GTA)  dctA ← C4‑dicarboxylic acid, orotate and citrate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,675,6140AT 5.6% 1131.8 / 44.4 322V398V (GTT→GTA) dctAC4‑dicarboxylic acid, orotate and citrate transporter
Reads supporting (aligned to +/- strand):  ref base A (176/128);  new base T (10/8);  total (186/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 327 total aligned reads displayed.

CAATTATGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT  >  CP009273/3675465‑3675763
                                                                                                                                                     |                                                                                                                                                     
     aTGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGAc                                                                                                                                                  >  2:3863747/1‑150 (MQ=255)
          cAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATgg                                                                                                                                             >  2:3566754/1‑150 (MQ=255)
          cAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATgg                                                                                                                                             >  2:718504/1‑150 (MQ=255)
           aaaCACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCTACGACAATGGt                                                                                                                                            >  2:4376893/1‑150 (MQ=255)
                 ccGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTAc                                                                                                                                      <  2:591075/150‑1 (MQ=255)
                  cGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACg                                                                                                                                     >  1:5419958/1‑150 (MQ=255)
                       tCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGtt                                                                                                                                <  1:1964802/150‑1 (MQ=255)
                        cGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTa                                                                                                                               <  1:5123394/150‑1 (MQ=255)
                        cGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTa                                                                                                                               >  1:713116/1‑150 (MQ=255)
                        cGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTa                                                                                                                               >  1:2481227/1‑150 (MQ=255)
                         ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTAc                                                                                                                              <  1:1344936/150‑1 (MQ=255)
                         ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTAc                                                                                                                              >  2:3192504/1‑150 (MQ=255)
                          gggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTAcc                                                                                                                             >  2:3680935/1‑150 (MQ=255)
                            gAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGa                                                                                                                           >  1:4152958/1‑150 (MQ=255)
                            gAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGACCAGTTCTTTCACCCACTTAGAAACGACAATGGTCGCTACGCCGTTACCGa                                                                                                                           >  2:2869947/1‑150 (MQ=255)
                               gggAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCa                                                                                                                        <  1:2245642/150‑1 (MQ=255)
                               gggAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCa                                                                                                                        <  1:3225417/150‑1 (MQ=255)
                                 gAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCTACGACAATGGTCGCTACGCCGTTACCGACCAgg                                                                                                                      <  1:1636485/150‑1 (MQ=255)
                                   gTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGtt                                                                                                                    >  1:3953793/1‑150 (MQ=255)
                                       gggCATAAGTGATGAGATTAAGAGGATAATTCGCGCGTTTTGCCATCCGGCTCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTc                                                                                                                <  1:3580244/150‑1 (MQ=255)
                                        ggCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCa                                                                                                               >  2:635272/1‑150 (MQ=255)
                                          cATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAgc                                                                                                             <  2:2329951/150‑1 (MQ=255)
                                             aaGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCa                                                                                                          >  1:2880096/1‑150 (MQ=255)
                                             aaGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCa                                                                                                          >  1:5176923/1‑150 (MQ=255)
                                                  atgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCTACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGc                                                                                                     >  2:1290737/1‑150 (MQ=255)
                                                  atgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGc                                                                                                     <  1:3704675/150‑1 (MQ=255)
                                                  atgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGATACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGc                                                                                                     >  2:3955308/1‑150 (MQ=255)
                                                   tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCt                                                                                                    >  2:5358891/1‑150 (MQ=255)
                                                    gagaTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCTACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                   >  1:2311640/1‑150 (MQ=255)
                                                     agaTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCTACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTc                                                                                                  >  1:5627920/1‑150 (MQ=255)
                                                      gaTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCt                                                                                                 >  2:456576/1‑150 (MQ=255)
                                                      gaTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCt                                                                                                 <  1:1453527/150‑1 (MQ=255)
                                                       aTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTg                                                                                                <  2:73199/150‑1 (MQ=255)
                                                       aTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTg                                                                                                >  1:3677980/1‑150 (MQ=255)
                                                       aTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTg                                                                                                <  2:396930/150‑1 (MQ=255)
                                                        ttAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGa                                                                                               >  1:792231/1‑150 (MQ=255)
                                                          aaGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGCTTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACa                                                                                             <  2:4570044/150‑1 (MQ=255)
                                                          aaGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACa                                                                                             >  1:4673550/1‑150 (MQ=255)
                                                          aaGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACa                                                                                             >  1:1857272/1‑150 (MQ=255)
                                                          aaGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGAGCACGTGCTTCTGTCa                                                                                             >  2:3368609/1‑150 (MQ=255)
                                                                aTAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGc                                                                                       >  1:2354293/1‑150 (MQ=255)
                                                                    ttCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTc                                                                                   <  2:3454704/150‑1 (MQ=255)
                                                                    ttCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTc                                                                                   <  1:939607/150‑1 (MQ=255)
                                                                      tgTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCATTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGa                                                                                 >  2:4141950/2‑150 (MQ=255)
                                                                      cGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGa                                                                                 <  2:2647030/150‑1 (MQ=255)
                                                                        tGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATa                                                                               <  2:1074960/150‑1 (MQ=255)
                                                                        tGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATa                                                                               <  1:5516958/150‑1 (MQ=255)
                                                                        tGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTGTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATa                                                                               >  2:397202/1‑150 (MQ=255)
                                                                          cGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTTGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATAcc                                                                             <  1:5581142/150‑1 (MQ=255)
                                                                             tttGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAg                                                                          <  2:5268061/150‑1 (MQ=255)
                                                                              ttGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAgg                                                                         >  1:3163347/1‑150 (MQ=255)
                                                                                gCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGAt                                                                       >  1:3848085/1‑150 (MQ=255)
                                                                                 ccATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATc                                                                      <  2:4270215/150‑1 (MQ=255)
                                                                                  cATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCa                                                                     <  2:5033049/150‑1 (MQ=255)
                                                                                  cATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCa                                                                     >  1:5238562/1‑150 (MQ=255)
                                                                                   aTCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAg                                                                    >  1:97133/1‑150 (MQ=255)
                                                                                   aTCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAg                                                                    >  1:2968728/1‑150 (MQ=255)
                                                                                       ggCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGcc                                                                <  1:983175/150‑1 (MQ=255)
                                                                                       ggCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACAGAGGATCAGCGcc                                                                >  2:3423044/1‑150 (MQ=255)
                                                                                          gcACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                             >  2:1799327/1‑150 (MQ=255)
                                                                                          gcACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                             >  2:2189990/1‑150 (MQ=255)
                                                                                          gcACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                             >  2:5411431/1‑150 (MQ=255)
                                                                                          gcACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGACGATACCGAGGATCAGCGCCAGa                                                             >  1:604789/1‑150 (MQ=255)
                                                                                              gATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCg                                                         <  1:4358661/150‑1 (MQ=255)
                                                                                               attattCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCga                                                        >  2:4424394/1‑149 (MQ=255)
                                                                                                  attCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAc                                                     >  2:4263100/1‑150 (MQ=255)
                                                                                                   ttCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                    <  2:1633940/150‑1 (MQ=255)
                                                                                                    tCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCg                                                   <  1:2440942/150‑1 (MQ=255)
                                                                                                        cacaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCaa                                               <  2:2555127/150‑1 (MQ=255)
                                                                                                         acaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCaaa                                              >  2:5220460/1‑150 (MQ=255)
                                                                                                         acaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCaaa                                              >  1:1025502/1‑150 (MQ=255)
                                                                                                           aTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATg                                            >  1:3631122/1‑150 (MQ=255)
                                                                                                            tCGTCCAGTTTTTTGTGGTCCAGTTCTTTAACCCACTTAGCAACGACAATTGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTACTGACATAAAGCGGTCGATACCGAGGATCAGAGCCAGAACCGCTACCGGCAAAttg                                           >  2:5596967/1‑148 (MQ=255)
                                                                                                              gTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGcc                                         >  1:4163374/1‑150 (MQ=255)
                                                                                                               tCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGccc                                        >  1:3071232/1‑150 (MQ=255)
                                                                                                                 cAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCAc                                      >  1:3903413/1‑150 (MQ=255)
                                                                                                                   gTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCg                                    >  1:1178629/1‑150 (MQ=255)
                                                                                                                        ttGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCagag                               >  2:1492987/1‑150 (MQ=255)
                                                                                                                        ttGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCagag                               <  1:5294042/150‑1 (MQ=255)
                                                                                                                        atgtgGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCACATGGCCCACCGCagag                               >  2:4328030/2‑150 (MQ=255)
                                                                                                                         tgtgGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCagaga                              >  1:2668361/1‑150 (MQ=255)
                                                                                                                          gtgGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCagagag                             >  2:3506755/1‑150 (MQ=255)
                                                                                                                           tgGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGc                            >  2:2955380/1‑150 (MQ=255)
                                                                                                                             gTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGt                          >  2:4622334/1‑150 (MQ=255)
                                                                                                                              tCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                         >  1:3723477/1‑150 (MQ=255)
                                                                                                                               ccAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcg                        <  2:1416049/150‑1 (MQ=255)
                                                                                                                                cAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcgc                       >  1:457668/1‑150 (MQ=255)
                                                                                                                                 aGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcgcc                      >  2:1519743/1‑150 (MQ=255)
                                                                                                                                 aGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcgcc                      >  1:5029973/1‑150 (MQ=255)
                                                                                                                                     cTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCa                  <  2:5176923/150‑1 (MQ=255)
                                                                                                                                      tttCACACACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAg                 <  2:2880096/150‑1 (MQ=255)
                                                                                                                                       ttCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGc                >  1:4068102/1‑150 (MQ=255)
                                                                                                                                        tCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCa               >  2:4947100/1‑150 (MQ=255)
                                                                                                                                         cACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCAc              >  1:1665677/1‑150 (MQ=255)
                                                                                                                                          aCCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACg             <  2:2481227/150‑1 (MQ=255)
                                                                                                                                              aCTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaa         >  2:4816596/1‑150 (MQ=255)
                                                                                                                                               cTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa        >  1:2121470/1‑150 (MQ=255)
                                                                                                                                                 tAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGc      >  1:3118262/1‑150 (MQ=255)
                                                                                                                                                   gCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCa    >  1:3434820/1‑150 (MQ=255)
                                                                                                                                                     aaCGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACt  >  1:3079831/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAATTATGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT  >  CP009273/3675465‑3675763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑