BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,721,480	T→G	7.0%	T418P (ACG→CCG)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,721,480	0	T	G	7.0%	841.2 / 10.7	319	T418P (ACG→CCG)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base T (116/171); new base G (27/4); total (143/176)
Fisher's exact test for biased strand distribution p-value = 5.08e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACG  >  CP009273/3721331‑3721628

tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGt                                                                                                                                                      >  2:4518225/1‑150 (MQ=255)

tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGt                                                                                                                                                      <  1:1702596/150‑1 (MQ=255)

    tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTAGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGACgg                                                                                                                                                  >  2:3262198/1‑150 (MQ=255)

     gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                 >  2:1215264/1‑150 (MQ=255)

        aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAt                                                                                                                                              <  1:2450140/150‑1 (MQ=255)

         aGCGGCATCACGCTGCGCATCTGGTAGAGGCGACTGTTCTACCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTaagc                                                                                                                                             <  2:5267702/150‑3 (MQ=255)

         aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                             <  1:4986707/150‑1 (MQ=255)

          gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCg                                                                                                                                            <  1:278910/150‑1 (MQ=255)

             gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCTATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                         >  1:4399976/1‑150 (MQ=255)

               aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                       >  2:492325/1‑150 (MQ=255)

               aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGTAAtggcggtg                                                                                                                                       >  2:5350761/1‑143 (MQ=255)

                tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGGCCTCGAgctgc                                                                                                                                      >  2:2585664/1‑150 (MQ=255)

                 aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGACGGTCAGCGGgctgct                                                                                                                                     >  1:4514784/1‑150 (MQ=255)

                    gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGACGGTACTCGCGCTGCTGAc                                                                                                                                  >  2:4028742/1‑150 (MQ=255)

                     cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCCCCCGGGGGGGTATCGGGCGGCTGAcc                                                                                                                                 >  2:492849/1‑150 (MQ=255)

                           aTCTGGTAGATGCGACTGTTCTACCGGTAGGTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                           <  2:3028112/150‑1 (MQ=255)

                           aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTGCGGTCGGCGGGCTGCTGGCCGCTggg                                                                                                                           >  1:2305093/1‑148 (MQ=255)

                            tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGGGCCCCCCGGGCGGGGCTCGGGCTGCGGGCCGCTgggg                                                                                                                          >  1:825386/1‑147 (MQ=255)

                            tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                          <  1:3308921/150‑1 (MQ=255)

                               ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGACGGTCATCGAGCTGCGGGCCGCTGAGATcc                                                                                                                       >  2:14297/1‑150 (MQ=255)

                                gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGGTGGCCGCGGGTATCCg                                                                                                                      >  1:931327/1‑150 (MQ=255)

                                 tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGCAGCGAGCTGGGGAGCGCTGATCTCCGc                                                                                                                     >  2:3749852/1‑150 (MQ=255)

                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTGCGGGAATCGAGCGGCTGGCCGGTGAGATCCGccc                                                                                                                   >  2:5212429/1‑149 (MQ=255)

                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGGAAGCGAGCTGCTGACCGCTGTGATCCGccc                                                                                                                   >  1:317763/1‑149 (MQ=255)

                                     tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                                                                                                 <  2:3200420/150‑1 (MQ=255)

                                     tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                                                                                                 <  2:340057/150‑1 (MQ=255)

                                        gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                              <  1:1581487/150‑1 (MQ=255)

                                         aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATAGCCGCCAGCATCt                                                                                                             >  2:3886647/1‑150 (MQ=255)

                                          cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATAGCCCCCCGTACGGTAATCGAGCTGCTGTCAGCTGAGATCCGCCCGCagcgg                                                                                                            >  1:2389941/1‑146 (MQ=255)

                                           tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGGAGGGTAATGGTGCGGGTGGGCGCGGGGATGCGCCAGCATCTGa                                                                                                           >  1:1410853/1‑150 (MQ=255)

                                            gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                          <  1:679713/150‑1 (MQ=255)

                                            gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                          <  1:1910698/150‑1 (MQ=255)

                                             ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                         <  1:1183965/150‑1 (MQ=255)

                                             ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                         <  1:4563677/150‑1 (MQ=255)

                                                   cGGTAGTTGCGGCAACAATTCCATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                   <  2:4880026/150‑1 (MQ=255)

                                                   cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                   <  2:1781570/150‑1 (MQ=255)

                                                    ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCCTCGAGCTGGGGGCCGCTGGGATCGGCCAGCATCTGACGCCCGtgc                                                                                                  >  2:1080091/1‑148 (MQ=255)

                                                     gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGGAGTCGGGCGGCGGACCGCTGCGGGCCGCCCGCAGCTGGCGCCGGTCCt                                                                                                 >  2:819909/1‑150 (MQ=255)

                                                       aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGGCCCCCCCGTCCGGGACGCGGGCTGCGGACCGGGGAGGGCCGCCCGGATCGGGCGCCAGTAGTcg                                                                                               >  1:510732/1‑149 (MQ=255)

                                                        gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                              <  2:1960362/150‑1 (MQ=255)

                                                          tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                            <  1:1121334/150‑1 (MQ=255)

                                                          tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                            <  2:4428917/150‑1 (MQ=255)

                                                              gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTGCCCCCCCGTCCGGGAATCGAGCTGCGGACCGCGGAGATCCGCCAGCATCTGACGCCAGTACTCACTAcgcg                                                                                        >  1:4199404/1‑150 (MQ=255)

                                                              gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGGGCGGTGATCGTGCTGGTGGGCGGGGGTGTGCGGCAGCGTCTGACGCCGGGACTCGCTAcggg                                                                                        >  1:2081460/1‑148 (MQ=255)

                                                                 acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                     <  2:2729129/150‑1 (MQ=255)

                                                                  caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                    <  1:3976162/150‑1 (MQ=255)

                                                                   aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTTCGGTACTCGGGGTGATGACCGGGGATCTCCGCCCGCATCTGACGCCAGTTCTCTCTACGcgccccg                                                                                   >  1:3768331/1‑150 (MQ=255)

                                                                     ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGTGATCGGGCGGCGGACCGCTGATATCCGCCAGCATCTGTCGCCCGTACTCACTTCGcgccccgcc                                                                                 >  1:5037704/1‑150 (MQ=255)

                                                                     ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                 <  1:492325/150‑1 (MQ=255)

                                                                       cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                               <  1:4275591/150‑1 (MQ=255)

                                                                          tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACTGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                            <  2:2175891/150‑1 (MQ=255)

                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                          <  1:4450700/150‑1 (MQ=255)

                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCGGATGTCCGCCGGCGTCTGACGCCAGTACTCACTGCGCGCCCCGCCCCCAATc                                                                          >  1:2954693/1‑150 (MQ=255)

                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCC‑TTCGGTAAAGGAGCAGCAGGCCCGTGTTATGCGCCCGCCTGTGACGCCAGTACTCCCGTCGCGGCCCGCCCCCCATCa                                                                         >  2:2968237/1‑150 (MQ=255)

                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                       <  1:3313405/150‑1 (MQ=255)

                                                                                aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                                                      <  1:4655536/150‑1 (MQ=255)

                                                                                 tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                     <  1:4145805/150‑1 (MQ=255)

                                                                                  ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                    <  1:3032425/150‑1 (MQ=255)

                                                                                           ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCGTCTGACGCCAGTGCTCACTTCGCGCCCCGCCCCCAATCGACGTAACACTGTGc                                                           >  2:2715132/1‑150 (MQ=255)

                                                                                             cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTACGGTAATCGTGCTGCTGTCCGCTGATATCCGCCCGCGTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                         >  1:2701889/1‑150 (MQ=255)

                                                                                             cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGTGCTGCGGGCCGCTGGTATCCGCCCGCATCTGCCGCCAGTGCTCACTACGCGCCCCGCCCCCAATCAGCGTAGCACTTTGCgg                                                         >  1:3868212/1‑150 (MQ=255)

                                                                                             cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCgg                                                         >  1:1723706/1‑150 (MQ=255)

                                                                                                cgctATCTTCGCCACCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCTTACGGTAATCGAGCAGCTGACCGCTGATATCCGCCAGCATCTGACGCCACTACTCACTACGAGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                      <  2:5654083/150‑1 (MQ=255)

                                                                                                cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                      <  1:3643711/150‑1 (MQ=255)

                                                                                                      cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                <  1:39109/150‑1 (MQ=255)

                                                                                                      cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                <  1:39422/150‑1 (MQ=255)

                                                                                                      cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                >  2:3668920/1‑150 (MQ=255)

                                                                                                       tGCGCCAGCCCTGCTGCGCCCAGGGCTGGCCCCACCTCCCCCCCCGTCCGGCAATCGAGCTGCCGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGAGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                               <  2:2777074/150‑1 (MQ=255)

                                                                                                         cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCCACGTAACACTTTGCGGGGTAATACCGCa                                             >  1:1767512/1‑150 (MQ=255)

                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCGGGCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTGTGCGGGTTTATACCGCAg                                            >  2:2341344/1‑150 (MQ=255)

                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                            <  1:574464/150‑1 (MQ=255)

                                                                                                           ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGTGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAATCTACGTGACACGTTGCGGGTTAATACCGCAgg                                           >  2:1912970/1‑150 (MQ=255)

                                                                                                           ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                           <  1:4617089/150‑1 (MQ=255)

                                                                                                              gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                        <  1:3093782/150‑1 (MQ=255)

                                                                                                              gCCTTGCTGCGCCCAGAGCTGGCCCCACCTCCCCCCCCGGACGGTAATCGAGCGGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                        <  2:3156988/150‑1 (MQ=255)

                                                                                                                 ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGGGATCGAGCTGCTGACCGCTGATATCCGCCGGCCTGTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCTACGTGACAGTTTGCGGGTGAATTCCGCAGGCATGCa                                     >  2:247016/1‑150 (MQ=255)

                                                                                                                  tgctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                    <  1:345566/150‑1 (MQ=255)

                                                                                                                      gcgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                <  1:1976467/150‑1 (MQ=255)

                                                                                                                         cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                             >  2:1023216/1‑150 (MQ=255)

                                                                                                                         cccAGTGCTGGCCCAACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACAACATcc                             <  1:4904638/150‑1 (MQ=255)

                                                                                                                          ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                            <  1:1536948/150‑1 (MQ=255)

                                                                                                                           cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                           >  1:1792912/1‑150 (MQ=255)

                                                                                                                            aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                          <  1:1356199/150‑1 (MQ=255)

                                                                                                                                 tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                     >  1:986151/1‑150 (MQ=255)

                                                                                                                                  ggCCCCCCATCCCCCCCCGTTCGTTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGTTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                    <  2:4684343/150‑1 (MQ=255)

                                                                                                                                  ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                    <  1:5350124/150‑1 (MQ=255)

                                                                                                                                  ggCCCCACAGCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                    <  2:1851666/150‑1 (MQ=255)

                                                                                                                                   gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATAACGCAGGCATGCACGACAGCCATGCCATCTg                   >  2:3503124/1‑150 (MQ=255)

                                                                                                                                     cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                 >  1:791498/1‑150 (MQ=255)

                                                                                                                                      ccccaTCCCCCCCCTTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACTCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACACCCATCCCATCTcccc                <  2:2803809/147‑2 (MQ=255)

                                                                                                                                      ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                >  2:132075/1‑150 (MQ=255)

                                                                                                                                      ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                <  1:1017982/150‑1 (MQ=255)

                                                                                                                                       cacaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg               >  2:5513085/1‑150 (MQ=255)

                                                                                                                                              ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa        <  1:5429277/150‑1 (MQ=255)

                                                                                                                                                ccccGTACGGTAATCGAGCTGCGGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGTCACACTTTGCGGTTTAATCCCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc      <  2:935687/150‑1 (MQ=255)

                                                                                                                                                 cccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc     >  2:4209566/1‑150 (MQ=255)

                                                                                                                                                 cccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc     >  2:5401912/1‑150 (MQ=255)

                                                                                                                                                  ccGTACGGTCCTCGCGCTGCTGACCGCCTAGATCCGCCAGCAACTGACGCCAGTACTCACGACGCGCCCCGCCCCCAAACAACGTAACACTTGGCGGTTTAATACCGCTGGCAGGCACGACATCCATGCCATCTGCCAGCGCATCACCCa    <  2:4076749/150‑1 (MQ=255)

                                                                                                                                                   cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCTTGCCATCTGCCAGCGCATAACCCAc   >  2:3865686/1‑150 (MQ=255)

                                                                                                                                                    gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg  >  2:62008/1‑150 (MQ=255)

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACG  >  CP009273/3721331‑3721628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`