Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 11.6% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG11.6% 721.9 / 27.6 313Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (101/167);  new base G (39/4);  total (140/173)
Fisher's exact test for biased strand distribution p-value = 2.15e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCT  >  CP009273/3721336‑3721631
                                                                                                                                                     |                                                                                                                                                  
gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTGGCGCCCCGTGCGGt                                                                                                                                                    >  1:2731956/1‑150 (MQ=255)
 aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTa                                                                                                                                                   <  1:3178534/150‑1 (MQ=255)
   aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAt                                                                                                                                                 <  1:2449737/150‑1 (MQ=255)
    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTATAACGGTAGTTGCGGCAACAATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGACAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                <  2:315895/150‑1 (MQ=255)
        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCTATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                            >  1:4399976/1‑150 (MQ=255)
        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCTATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                            >  1:1565961/1‑150 (MQ=255)
          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGGAAGCGAgctg                                                                                                                                          >  2:5350124/1‑150 (MQ=255)
          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                          >  2:492325/1‑150 (MQ=255)
            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                        <  2:4719404/150‑1 (MQ=255)
            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGTCTTCGGgctggg                                                                                                                                        >  1:4514773/1‑148 (MQ=255)
               gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGTGctgctggc                                                                                                                                     >  1:948891/1‑148 (MQ=255)
               gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGTACTCGGGctgctggc                                                                                                                                     >  1:3065362/1‑148 (MQ=255)
               gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGACGGTACTCGCGCTGCTGAc                                                                                                                                     >  2:4028742/1‑150 (MQ=255)
                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCCCCCGGGGGGGTATCGGGCGGCTGAcc                                                                                                                                    >  2:492849/1‑150 (MQ=255)
                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAcc                                                                                                                                    <  2:3226907/150‑1 (MQ=255)
                    gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                                                                <  1:738146/150‑1 (MQ=255)
                      aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                              <  1:4919073/150‑1 (MQ=255)
                      aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                              <  2:3028132/150‑1 (MQ=255)
                      aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                              <  2:2776151/150‑1 (MQ=255)
                       tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                             <  1:3308921/150‑1 (MQ=255)
                        cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                            <  1:1532471/150‑1 (MQ=255)
                         tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCCCCCGTACGGGAATCGAGCTGGTGAGCGCGGatgtg                                                                                                                           >  1:5593633/1‑147 (MQ=255)
                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                          <  2:1939475/150‑1 (MQ=255)
                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGCCGGGCCGCGGGCTGCTGACCGCGGAGATcc                                                                                                                          >  2:14187/1‑150 (MQ=255)
                            tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGCAGCGAGCTGGGGAGCGCTGATCTCCGc                                                                                                                        >  2:3749852/1‑150 (MQ=255)
                             aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCCCGCGTACGGTAATCGAGCGGCTGGCCGCGGTGATCCGcc                                                                                                                       >  2:5641243/1‑150 (MQ=255)
                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                      <  2:2024341/150‑1 (MQ=255)
                                tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                                                                                                    <  2:340057/150‑1 (MQ=255)
                                   gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                 <  1:384250/150‑1 (MQ=255)
                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                <  2:5658000/150‑1 (MQ=255)
                                     cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTTCGCCAGCCTTGCTTCGCCCAGTGCTGGCACCACATCACCCCCCTTACGGTAAGCGAGCTGATGGACGCTGATTGCGGCCAGCGTCTg                                                                                                               >  1:5213621/1‑150 (MQ=255)
                                     cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCGCCCCCGGACGGTAATCGTGCTGCTGACCGCGGATAGCCGCCAGCATCTg                                                                                                               >  1:2873870/1‑150 (MQ=255)
                                     cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTg                                                                                                               <  2:366217/150‑1 (MQ=255)
                                      tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGGAGGGTAATGGTGCGGGTGGGCGCGGGGATGCGCCAGCATCTGa                                                                                                              >  1:1410853/1‑150 (MQ=255)
                                      tGTTCTAACGGTAGTTGCGGCAACAAGTCACTGAGCGATTGCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                              <  2:2969956/150‑1 (MQ=255)
                                       gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                             <  2:1109974/150‑1 (MQ=255)
                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                            <  1:1183965/150‑1 (MQ=255)
                                            aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCGCCCGGGCGGGCCTCGCGGGGCTGGCCGCGGGGATCCGCCCGCCTCTGACGCCAg                                                                                                        >  2:1068498/1‑150 (MQ=255)
                                              cGGTAGTTGCGGCAACAATTCCATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                      <  2:1781022/150‑1 (MQ=255)
                                                gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGGAGTCGGGCGGCGGACCGCTGCGGGCCGCCCGCAGCTGGCGCCGGTCCt                                                                                                    >  2:819909/1‑150 (MQ=255)
                                                 tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                                                   <  1:417974/150‑1 (MQ=255)
                                                 tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                                                   <  1:2465149/150‑1 (MQ=255)
                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCGCCGCCGTGCGGTGATGGTGCGGGTGGCGGCTGAGTGGCGCCGGCGGCTGGCGCCAGTGCTct                                                                                                  >  1:510727/1‑149 (MQ=255)
                                                    ttGCGGCAACAATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                                <  1:4869487/150‑1 (MQ=255)
                                                    ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                                <  2:2401833/150‑1 (MQ=255)
                                                     tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                               <  2:4428917/150‑1 (MQ=255)
                                                     tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                               <  1:1121334/150‑1 (MQ=255)
                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                              <  1:1912851/150‑1 (MQ=255)
                                                         gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGGAATCGAGCTGCTGACCGCTGCTATCCGCCCGCATCTGCCGCCAGTAGGCACGAcgcg                                                                                           >  2:4556164/1‑150 (MQ=255)
                                                            acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                        <  2:2729108/150‑1 (MQ=255)
                                                             caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                       <  1:3976162/150‑1 (MQ=255)
                                                             caaTTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                       <  1:4558265/150‑1 (MQ=255)
                                                              aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                                                      <  2:2796482/150‑1 (MQ=255)
                                                               aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGTCGGGAATCGAGCTGCTGACCGCTGTGATGCGGCGGCATCTGTCGCCAGTCCTCGCTACGcgccccgc                                                                                     >  2:508029/1‑150 (MQ=255)
                                                                ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                    <  1:492848/150‑1 (MQ=255)
                                                                  cAATGAGCGTTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                  <  2:1251350/150‑1 (MQ=255)
                                                                  cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                  <  1:4275591/150‑1 (MQ=255)
                                                                      gAGCGCTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACTGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                              <  2:524531/150‑1 (MQ=255)
                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                             <  1:4450700/150‑1 (MQ=255)
                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCGGATGTCCGCCGGCGTCTGACGCCAGTACTCACTGCGCGCCCCGCCCCCAATc                                                                             >  1:2954693/1‑150 (MQ=255)
                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGTAATCGGGCTGCTGACCGGGGATATCCGCCAGCATCTGGCGCCAGTACGCACTACGCGCCCCGCCCCCAATCaa                                                                           >  1:1777563/1‑150 (MQ=255)
                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGAATCGTGCTGCTGACCGCTGATCGCCGCGGGCGGCTGACGCCAGTGCGCCCTACGCGCCCCGCCCCCacgcccc                                                                          >  1:539860/1‑144 (MQ=255)
                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                          <  1:3313405/150‑1 (MQ=255)
                                                                           aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGTCATCGAGCTGCTGACCGCTGGTATCCGGCAGCATCTGGCGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACg                                                                         >  1:1989987/1‑150 (MQ=255)
                                                                           aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                                                         <  1:4655536/150‑1 (MQ=255)
                                                                            tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACga                                                                        >  2:190811/1‑149 (MQ=255)
                                                                            tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                        <  1:4145731/150‑1 (MQ=255)
                                                                              tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                      <  1:171821/150‑1 (MQ=255)
                                                                               ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCATGTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                     >  1:4988034/1‑150 (MQ=255)
                                                                                tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGATCGGGCTGGTGAGCGCTGATATCCGCCCGCATCTGACGCCGGTAGTCACGACGCGCCCCGCCCCCAATCAACGTaagc                                                                    >  1:988561/1‑148 (MQ=255)
                                                                                 ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGTAATCGAGCTGCTGTCCGCGGATAGCCGCCCGCATCGGACGCCCGTACTCGCGACGCGCCCCGCCCCCCAGCCACGttgcac                                                                   >  1:3104564/1‑145 (MQ=255)
                                                                                 ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGGGCTGCTGTCCGCTGGTGGCCGCCAGCATCTGTCGCCAGTACTCGCTACGCGCCCCGCCCCCAATCAACGTAgcac                                                                   >  1:896935/1‑150 (MQ=255)
                                                                                  tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                  <  1:671063/150‑1 (MQ=255)
                                                                                       tCGCCGCGATCTGCGCCAGCCTTTCTGCGCCCAGTGCTGGCCCCACCTCACCCCCCGTTCTCTAATCGAGCTTCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACACACAACGCGCCCCGCCCCCAATCAAAGTCAAACTTTGCg                                                             <  2:3905699/150‑1 (MQ=255)
                                                                                        cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGTGCTGCGGGCCGCTGGTATCCGCCCGCATCTGCCGCCAGTGCTCACTACGCGCCCCGCCCCCAATCAGCGTAGCACTTTGCgg                                                            >  1:3868212/1‑150 (MQ=255)
                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCGCAATCCACGTTACCCTTTGCggg                                                           >  2:5356358/1‑149 (MQ=255)
                                                                                           cgcgATCTGCGCCCGCCGTGCTGCGCCCAGTGCTGGCCCCACCTCACCCCCCGGACGGGACTCGAGCTGCTGACCGCTGAGAGCCGCCAGCATCGGCCGCCAGTACGCACTCCGCTCCCCGCCCCCACTCACCGTAACACTTTGCGGttt                                                         <  2:3867303/150‑1 (MQ=255)
                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                         <  1:3643711/150‑1 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                      <  1:1824010/150‑1 (MQ=255)
                                                                                                tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                    <  1:2096136/150‑1 (MQ=255)
                                                                                                 cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGGGCTGCTGACCGCTGATATCCGCCTGCAGCTGACGCCAGGACTCACTACGCGCCCCGCCCGCAGGCTACGTGACACGTTGCGGTTGAGTAcc                                                   >  2:2242421/1‑150 (MQ=255)
                                                                                                 cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                   <  1:39422/150‑1 (MQ=255)
                                                                                                 cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                   <  1:39109/150‑1 (MQ=255)
                                                                                                      ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                              <  1:4616925/150‑1 (MQ=255)
                                                                                                         gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                           <  1:231293/150‑1 (MQ=255)
                                                                                                             tgctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                       <  1:345566/150‑1 (MQ=255)
                                                                                                                    cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                                >  2:264421/1‑150 (MQ=255)
                                                                                                                       aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                             <  1:1356199/150‑1 (MQ=255)
                                                                                                                       aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAGGCACGCCATCCATg                             >  2:2965212/1‑150 (MQ=255)
                                                                                                                             ggCCCCACATCCCCCCACGTACGGTACTCGCGCTGCTGAACGCTGAGATCCGGCAGCATGTGACGCCAGTACTCACTACGCGCCCCGCCACCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACTACATCCATGCCATCt                       <  2:2526275/150‑1 (MQ=255)
                                                                                                                              gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATAACGCAGGCATGCACGACAGCCATGCCATCTg                      >  2:3503124/1‑150 (MQ=255)
                                                                                                                                cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                    >  1:791559/1‑150 (MQ=255)
                                                                                                                                 ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                   <  1:1017982/150‑1 (MQ=255)
                                                                                                                                  cacaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg                  >  2:5513085/1‑150 (MQ=255)
                                                                                                                                       ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt             <  1:1739987/150‑1 (MQ=255)
                                                                                                                                        cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa            >  2:1813569/1‑150 (MQ=255)
                                                                                                                                           ccccGTACGGTAATCGAGCTGCGGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGTCACACTTTGCGGTTTAATCCCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc         <  2:935687/150‑1 (MQ=255)
                                                                                                                                            cccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc        >  2:4209566/1‑150 (MQ=255)
                                                                                                                                             ccGTACGGTCCTCGCGCTGCTGACCGCCTAGATCCGCCAGCAACTGACGCCAGTACTCACGACGCGCCCCGCCCCCAAACAACGTAACACTTGGCGGTTTAATACCGCTGGCAGGCACGACATCCATGCCATCTGCCAGCGCATCACCCa       <  2:4076749/150‑1 (MQ=255)
                                                                                                                                                  cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt  >  2:688127/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCT  >  CP009273/3721336‑3721631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑