Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,507 T→G 8.8% I409L (ATC→CTC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,5070TG 8.8% 811.4 / 11.5 295I409L (ATC→CTC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (107/153);  new base G (29/5);  total (137/158)
Fisher's exact test for biased strand distribution p-value = 8.23e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

ATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAT  >  CP009273/3721358‑3721656
                                                                                                                                                     |                                                                                                                                                     
aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                                                       <  1:4919073/150‑1 (MQ=255)
    ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                                                   <  2:1939475/150‑1 (MQ=255)
       aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                                                                                <  2:5562128/150‑1 (MQ=255)
        gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                                               <  2:2024341/150‑1 (MQ=255)
            cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                                           <  2:3187597/150‑1 (MQ=255)
             gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCTTCCCCCCCCGTGCGGGAGTCGGGCTGGTGGCGGGGGAGGTCCGCCAGCAtg                                                                                                                                          >  2:2585744/1‑149 (MQ=255)
             gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                                          <  1:384264/150‑1 (MQ=255)
              aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                                         <  2:5657888/150‑1 (MQ=255)
              aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATAGCCGCCAGCATCt                                                                                                                                         >  2:3886647/1‑150 (MQ=255)
               cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTTCGCCAGCCTTGCTTCGCCCAGTGCTGGCACCACATCACCCCCCTTACGGTAAGCGAGCTGATGGACGCTGATTGCGGCCAGCGTCTg                                                                                                                                        >  1:5213621/1‑150 (MQ=255)
               cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATAGCCCCCCGTACGGTAATCGAGCTGCTGTCAGCTGAGATCCGCCCGCagcgg                                                                                                                                        >  1:2389941/1‑146 (MQ=255)
                tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCGGGCCGCTGATATCCGCCAGCAGCTGa                                                                                                                                       >  1:1410710/1‑150 (MQ=255)
                 gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                                                      <  1:679713/150‑1 (MQ=255)
                 gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                                                      <  1:1910663/150‑1 (MQ=255)
                 gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                                                      <  1:1739163/150‑1 (MQ=255)
                  ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                                                     <  2:2747949/150‑1 (MQ=255)
                        cGGTAGTTGCGGCAACAATTCCATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                                               <  2:4880026/150‑1 (MQ=255)
                        cGGTAGTTGCGGCAACAATTCCATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                                               <  2:1781022/150‑1 (MQ=255)
                         ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCCTCGAGCTGGGGGCCGCTGGGATCGGCCAGCATCTGACGCCCGtgc                                                                                                                              >  2:1080091/1‑148 (MQ=255)
                            aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCGCCGCCGTGCGGTGATGGTGCGGGTGGCGGCTGAGTGGCGCCGGCGGCTGGCGCCAGTGCTct                                                                                                                           >  1:510727/1‑149 (MQ=255)
                            aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGGGTGCGGGCTGCTGAGCGCTGCTATCGGCGAGCGGCTGGCGGCAGTaggca                                                                                                                           >  2:2722241/1‑146 (MQ=255)
                              ttGCGGCAACAATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                                                         <  1:4869487/150‑1 (MQ=255)
                              ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                                                         <  2:2401833/150‑1 (MQ=255)
                               tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                                                        <  1:1121334/150‑1 (MQ=255)
                                   gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTGCCCCCCCGTCCGGGAATCGAGCTGCGGACCGCGGAGATCCGCCAGCATCTGACGCCAGTACTCACTAcgcg                                                                                                                    >  1:4199404/1‑150 (MQ=255)
                                   gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGGGCGGTGATCGTGCTGGTGGGCGGGGGTGTGCGGCAGCGTCTGACGCCGGGACTCGCTAcggg                                                                                                                    >  1:2081460/1‑148 (MQ=255)
                                   gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGGAATCGAGCTGCTGACCGCTGCTATCCGCCCGCATCTGCCGCCAGTAGGCACGAcgcg                                                                                                                    >  2:4556164/1‑150 (MQ=255)
                                     aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGTGATCGAGCTGCGGTCCGCGGGGGTCCGCCAGCATCTGACGCCAGTACGCACTACGcgcc                                                                                                                  >  1:390788/1‑150 (MQ=255)
                                       caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                                                <  1:1606819/150‑1 (MQ=255)
                                         aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGTCGGGAATCGAGCTGCTGACCGCTGTGATGCGGCGGCATCTGTCGCCAGTCCTCGCTACGcgccccgc                                                                                                              >  2:508029/1‑150 (MQ=255)
                                            cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                                           <  1:4275591/150‑1 (MQ=255)
                                            cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGAGCGGCTGACCGCTGTTATCCGCCAGCGTCGGGCGCCAGTAGGCACTACGCgccccgcccc                                                                                                           >  1:428654/1‑150 (MQ=255)
                                              aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTTATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCCGGCGGCACTACGCGCCCCGcccccg                                                                                                         >  1:1774778/1‑149 (MQ=255)
                                               tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGACGGTCATCGCGCTGCTGCCCGCGGAGCTCCGCCCGCGTCTGACGCCCGTCCTCACTACGCGCCCCGcccccca                                                                                                        >  2:1287290/1‑148 (MQ=255)
                                                gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                                                       <  2:4228760/150‑1 (MQ=255)
                                                gAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCATCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                                                       <  2:523822/150‑1 (MQ=255)
                                                 aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                                                      <  1:4450700/150‑1 (MQ=255)
                                                 aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGGCGGTAGTCGAGCTGCGGACCGCGGGGATCGGCCAGCGTCTGGCGCCAGTACTCACTCGGGGGCGCGcccccgagc                                                                                                      >  1:2954796/1‑146 (MQ=255)
                                                  gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTTCTCACTACGCGCCCCGCCCCCacgcc                                                                                                     >  1:4140406/1‑146 (MQ=255)
                                                    gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCACCGTACGGTAATCGCGCTGCTGACCTCTGATGTCCGCCAGCATAAGACGCCAGTCATCTCTAGTCGCCCCGCGCCCCATCAAc                                                                                                   >  2:3517810/1‑150 (MQ=255)
                                                    gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGAATCGTGCTGCTGACCGCTGATCGCCGCGGGCGGCTGACGCCAGTGCGCCCTACGCGCCCCGCCCCCacgcccc                                                                                                   >  1:539860/1‑144 (MQ=255)
                                                    gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                                                   <  1:2256142/150‑1 (MQ=255)
                                                    gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                                                   <  1:3313405/150‑1 (MQ=255)
                                                      tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACga                                                                                                 >  2:190811/1‑149 (MQ=255)
                                                      tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                                                 <  1:4145805/150‑1 (MQ=255)
                                                       ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                                                <  1:3032425/150‑1 (MQ=255)
                                                        tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                                               <  1:171821/150‑1 (MQ=255)
                                                         ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCATGTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                                              >  1:4988034/1‑150 (MQ=255)
                                                           ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCGGATATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAgcac                                                                                            >  1:3742870/1‑150 (MQ=255)
                                                           ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGGGCTGCTGTCCGCTGGTGGCCGCCAGCATCTGTCGCCAGTACTCGCTACGCGCCCCGCCCCCAATCAACGTAgcac                                                                                            >  1:896935/1‑150 (MQ=255)
                                                             ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                                                          <  1:4778639/150‑1 (MQ=255)
                                                             ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                                                          <  1:2898701/150‑1 (MQ=255)
                                                              gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                                                         <  1:1171356/150‑1 (MQ=255)
                                                               aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGTCGGGAATCGAGGTGCTGACCGCTGATCTCCGCCAGCGTCGGACGCCAGTACGCTCTGCGCGCCCCGCCCCCAAGCACCGTGACCCGTTg                                                                                        >  2:445022/1‑150 (MQ=255)
                                                               aTTCGACGCGATTTGCGCCCGCCTGGCTGCGCCCAGTGCTGCCCCCACAGCCCCCCCCGTACGCTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACTTAACACTTTg                                                                                        <  2:3459407/150‑1 (MQ=255)
                                                                 tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                                                      <  1:4518225/150‑1 (MQ=255)
                                                                   gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATCCCCCCCCGGACGGGACGCGGGGTGCTGACCGCTGATGGCCGCCAGCCGCGGTCGCCAGGGCTGACTGCGCGCCCCGCCCCCCAGCAGCGGACCACTTTGCGGt                                                                                    >  2:5356088/1‑150 (MQ=255)
                                                                          tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                                             <  1:4637852/150‑1 (MQ=255)
                                                                           cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGATGTCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCACTCAACGTCACCCGTTGCGGGTTtatagc                                                                            >  2:5496320/1‑148 (MQ=255)
                                                                           cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                                            <  1:39169/150‑1 (MQ=255)
                                                                            tGCGCCAGCCCTGCTGCGCCCAGGGCTGGCCCCACCTCCCCCCCCGTCCGGCAATCGAGCTGCCGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGAGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                                                           <  2:2777074/150‑1 (MQ=255)
                                                                              cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGGCACGTTGCGGTTGAATACCGCa                                                                         >  1:1942859/1‑150 (MQ=255)
                                                                              cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                                                                         <  1:3220977/150‑1 (MQ=255)
                                                                               gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                                                        <  1:574464/150‑1 (MQ=255)
                                                                                    cagtgctgcGCCCAGTGCTGGCCCCACAGCCCCCCCCTGACGTTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTTCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                                                   <  2:3073201/147‑1 (MQ=255)
                                                                                      ttGCTGCGCCCAGTGCTGGCCCCACATCTCCCCCCGTACGTTAATCGAGCTGCTGACCGCTGATATCGGCCAGCATCTGTCTCCAGTACTCACTACGCGCCCCGACCCAAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                                                 <  2:1783388/150‑1 (MQ=255)
                                                                                              cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                                                         >  2:1023216/1‑150 (MQ=255)
                                                                                              cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACAACATcc                                                         <  1:1216660/150‑1 (MQ=255)
                                                                                               ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                                        <  1:1536948/150‑1 (MQ=255)
                                                                                                cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                                       >  1:1792912/1‑150 (MQ=255)
                                                                                                 aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGCCATCCATg                                                      >  2:2965356/1‑150 (MQ=255)
                                                                                                  ccgccggCCCCACCTCCCCCCCCGCACGCAAACCGAGCAGCTCCCCGCTGATCTCCGCCCGCATCTGACTCCACAACGCCCGCCGCGCCCCGCCCCCACTCCACCTACCACTTTTCTGTTGACTACCGCAGGCATGCACGACATCCATGc                                                     <  2:2190967/145‑1 (MQ=255)
                                                                                                      tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                                                 >  1:986151/1‑150 (MQ=255)
                                                                                                       ggCCCCACATCCCCCCACGTACGGTACTCGCGCTGCTGAACGCTGAGATCCGGCAGCATGTGACGCCAGTACTCACTACGCGCCCCGCCACCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACTACATCCATGCCATCt                                                <  2:2526275/150‑1 (MQ=255)
                                                                                                          cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                                             >  1:791559/1‑150 (MQ=255)
                                                                                                           ccccaTCCCCCCCCTTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACTCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACACCCATCCCATCTcccc                                            <  2:2803809/147‑2 (MQ=255)
                                                                                                           ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                                            <  1:1017982/150‑1 (MQ=255)
                                                                                                           ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAAATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAAGTAACACTTTGAGGTTTAATACCGCAGGCATGCACTACATCCATTACATCTGACa                                            >  2:3003231/1‑150 (MQ=255)
                                                                                                             acaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc                                          >  2:964969/1‑150 (MQ=255)
                                                                                                                     ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATATcc                                  >  2:328528/1‑150 (MQ=255)
                                                                                                                     ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc                                  <  1:4567954/150‑1 (MQ=255)
                                                                                                                      cccGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc                                 <  2:1541919/150‑1 (MQ=255)
                                                                                                                       ccGTACGGTCCTCGCGCTGCTGACCGCCTAGATCCGCCAGCAACTGACGCCAGTACTCACGACGCGCCCCGCCCCCAAACAACGTAACACTTGGCGGTTTAATACCGCTGGCAGGCACGACATCCATGCCATCTGCCAGCGCATCACCCa                                <  2:4076749/150‑1 (MQ=255)
                                                                                                                        cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCTTGCCATCTGCCAGCGCATAACCCAc                               >  2:3865686/1‑150 (MQ=255)
                                                                                                                         gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg                              >  2:820806/1‑150 (MQ=255)
                                                                                                                            cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt                           >  2:688068/1‑150 (MQ=255)
                                                                                                                                aaTCGAGCTGCTGCCCGCTGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCGCCAATCTCCGTTACACTTTGCGGTTTAATACCGCAGGCATGCTCGACATCCATGCCATCTGCCAGCGCATTACCCACGCCTTCCa                       <  2:3732921/150‑1 (MQ=255)
                                                                                                                                aaTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATTCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCa                       >  1:1497265/1‑150 (MQ=255)
                                                                                                                                 ctCGCGCGGCTGACCGCAGATAACCGCCAGCATCTGACGCCAGCACTCCCGACGCGCCCCGCCCCCAAACACCCGCCCACTTGGCGGATCAAGACCGCACGCACGCCCGACCTCCATGCCATCGGCCCCCGCAGAACCCCCGCCTTcccc                      <  2:4125091/149‑3 (MQ=255)
                                                                                                                                  tCGAGCTGCTGACCGCTGATATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGc                     <  2:3766758/150‑1 (MQ=255)
                                                                                                                                        tgctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCt               >  1:3434904/1‑150 (MQ=255)
                                                                                                                                          ctgACCGCTGATATCCGCCATCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCACCCATCGTAACACTTTGCGGTTTTATACCGCAGGCATGCACGACATCCATGCCATCTTCCAGCGCATATCCCACGCCTTCCAGCACTGCTcg             <  2:764326/150‑1 (MQ=255)
                                                                                                                                          ctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcg             >  1:2641015/1‑150 (MQ=255)
                                                                                                                                           tgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcgc            >  2:1195895/1‑150 (MQ=255)
                                                                                                                                           tgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcgc            >  1:3113028/1‑150 (MQ=255)
                                                                                                                                            gACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcgcg           >  1:3956681/1‑150 (MQ=255)
                                                                                                                                               cGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCa        <  2:1065963/150‑1 (MQ=255)
                                                                                                                                                 cTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGt      >  1:5271595/1‑150 (MQ=255)
                                                                                                                                                   gATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTc    >  2:4601513/1‑150 (MQ=255)
                                                                                                                                                    atatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGATCGCGCCAGTTCa   >  1:3836342/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAT  >  CP009273/3721358‑3721656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑