Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,837,177 C→T 43.6% intergenic (‑28/‑147) yicO ← / → ade putative adenine permease/cryptic adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,837,1770CT43.6% 195.1 / inf 360intergenic (‑28/‑147)yicO/adeputative adenine permease/cryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base C (93/109);  new base T (86/70);  total (179/181)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 369 total aligned reads displayed.

AAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGAAAAATATG  >  CP009273/3837030‑3837326
                                                                                                                                                   |                                                                                                                                                     
aaaaGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATtcc                                                                                                                                                     <  1:3997412/150‑1 (MQ=255)
aaaaGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCAGTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATccc                                                                                                                                                     <  2:2686500/150‑1 (MQ=255)
   aGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTg                                                                                                                                                  >  1:1689424/1‑150 (MQ=255)
    gTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGc                                                                                                                                                 >  2:37437/1‑150 (MQ=255)
     tGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCt                                                                                                                                                <  1:1054253/150‑1 (MQ=255)
     tGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCt                                                                                                                                                <  1:1631402/150‑1 (MQ=255)
       gTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCttt                                                                                                                                              >  2:322385/1‑150 (MQ=255)
         cATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTg                                                                                                                                            <  2:2997150/150‑1 (MQ=255)
            ccccGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTaa                                                                                                                                         <  1:5383511/150‑1 (MQ=255)
             cccGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTaaa                                                                                                                                        >  1:4650703/1‑150 (MQ=255)
             cccGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTaaa                                                                                                                                        <  1:1768905/150‑1 (MQ=255)
             cccGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTaaa                                                                                                                                        <  1:5648699/150‑1 (MQ=255)
               cGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAAc                                                                                                                                      >  2:2935904/1‑150 (MQ=255)
                   atcaatTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAAt                                                                                                                                  <  1:4503158/150‑1 (MQ=255)
                   atcaatTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAAt                                                                                                                                  >  2:1227545/1‑150 (MQ=255)
                     caatTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGa                                                                                                                                >  1:5235207/1‑150 (MQ=255)
                       atTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGaca                                                                                                                              <  1:1900888/150‑1 (MQ=255)
                       atTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATAGTCATTATTCATTTTTTTGTCCAGTGGAGGAGATTTAATCCCTTGATTTTGTAAAACAAATGaca                                                                                                                              >  2:1582005/1‑150 (MQ=255)
                           tgtgCGGACTGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGAcaacaa                                                                                                                          <  1:1016706/150‑1 (MQ=255)
                                ggACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGtt                                                                                                                     <  1:1533352/150‑1 (MQ=255)
                                 gACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTc                                                                                                                    >  2:3448875/1‑150 (MQ=255)
                                  aCGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCa                                                                                                                   <  1:808407/150‑1 (MQ=255)
                                   cGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAg                                                                                                                  >  1:3468812/1‑150 (MQ=255)
                                   cGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAg                                                                                                                  <  2:1987900/150‑1 (MQ=255)
                                   cGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCAGTTTTTTGTCCATTGGAGGAGATTTTATCCCTTGCTTTTGTATATCAAATGACAACAACGGTTCAg                                                                                                                  >  2:4814688/1‑150 (MQ=255)
                                    ggtggtCCCATGCTGATGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGt                                                                                                                 <  1:3152759/150‑1 (MQ=255)
                                    ggtggtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGt                                                                                                                 >  1:3953492/1‑150 (MQ=255)
                                    ggtggtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGt                                                                                                                 >  1:2792508/1‑150 (MQ=255)
                                    ggtggtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATTACAACAACGGTTCAGt                                                                                                                 >  2:1097299/1‑150 (MQ=255)
                                    ggtggtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGt                                                                                                                 >  2:3936638/1‑150 (MQ=255)
                                        gtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTATTCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATa                                                                                                             <  2:4401483/150‑1 (MQ=255)
                                        gtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATa                                                                                                             >  2:3612382/1‑150 (MQ=255)
                                        gtCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATa                                                                                                             >  2:5078834/1‑150 (MQ=255)
                                         tCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATaa                                                                                                            <  1:3143705/150‑1 (MQ=255)
                                            cATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTa                                                                                                         <  1:3797490/150‑1 (MQ=255)
                                             aTGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTAt                                                                                                        <  2:1038403/150‑1 (MQ=255)
                                                cTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATcac                                                                                                     >  1:3864218/1‑150 (MQ=255)
                                                cTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATcac                                                                                                     >  2:3174038/1‑150 (MQ=255)
                                                    ggTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACAttt                                                                                                 <  2:1694586/150‑1 (MQ=255)
                                                        gTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAAt                                                                                             >  1:4578988/1‑150 (MQ=255)
                                                        gTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAAt                                                                                             <  2:88551/150‑1 (MQ=255)
                                                                aatCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACAtt                                                                                     >  2:5313944/1‑150 (MQ=255)
                                                                aatCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATGGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACAtt                                                                                     >  2:813731/1‑150 (MQ=255)
                                                                aatCGCGAAAGCGTCCCTGATGCATGACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACAtt                                                                                     <  2:3999523/150‑1 (MQ=255)
                                                                 atCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTa                                                                                    <  1:4077134/150‑1 (MQ=255)
                                                                     cgAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATgg                                                                                <  1:2532736/150‑1 (MQ=255)
                                                                      gAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGa                                                                               >  2:4306072/1‑150 (MQ=255)
                                                                      gAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGa                                                                               >  1:676483/1‑150 (MQ=255)
                                                                       aaaGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGat                                                                              <  2:4146060/150‑1 (MQ=255)
                                                                        aaGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGata                                                                             <  2:413094/150‑1 (MQ=255)
                                                                           cGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTc                                                                          >  2:5169345/1‑150 (MQ=255)
                                                                             tCCCAGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGATATTAAATTCCTTGCTTATGTAAAAAAAATGACAAAAACGGTTCAGTGATAATTATCACATTTCAATTGAAAATTAATGGATATACtt                                                                        >  1:3058637/1‑150 (MQ=255)
                                                                                   aTTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTtaataa                                                                  >  2:4936797/1‑150 (MQ=255)
                                                                                    ttCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTaataat                                                                 <  2:183787/150‑1 (MQ=255)
                                                                                    ttCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTaataat                                                                 <  2:5252997/150‑1 (MQ=255)
                                                                                        ttACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTcg                                                             <  2:252164/150‑1 (MQ=255)
                                                                                            tCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGaa                                                         >  2:2879764/1‑149 (MQ=255)
                                                                                             cACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACg                                                        <  1:5537089/150‑1 (MQ=255)
                                                                                               cGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGtt                                                      >  2:2468763/1‑150 (MQ=255)
                                                                                                gTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGttt                                                     >  1:1565587/1‑150 (MQ=255)
                                                                                                gTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTGTTTAATAATCTCGCGACGttt                                                     <  2:1135026/150‑1 (MQ=255)
                                                                                                 tAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTc                                                    >  2:5619286/1‑150 (MQ=255)
                                                                                                   aTCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCtt                                                  <  2:5109015/150‑1 (MQ=255)
                                                                                                    tCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCttt                                                 >  2:2455718/1‑150 (MQ=255)
                                                                                                      ggTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTAt                                               <  2:4508916/150‑1 (MQ=255)
                                                                                                      ggTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTAt                                               <  2:3898419/150‑1 (MQ=255)
                                                                                                       gTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATg                                              <  1:1401009/150‑1 (MQ=255)
                                                                                                          ttGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGata                                           <  2:2266952/150‑1 (MQ=255)
                                                                                                          ttGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGata                                           >  1:4196605/1‑150 (MQ=255)
                                                                                                           tGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGataa                                          >  1:2487422/1‑150 (MQ=255)
                                                                                                            gTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGataaa                                         >  1:2429642/1‑150 (MQ=255)
                                                                                                             tCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGataaat                                        >  2:1775241/1‑150 (MQ=255)
                                                                                                             tCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGataaat                                        >  1:759812/1‑150 (MQ=255)
                                                                                                                ttattCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAaataat                                     <  2:5489438/150‑1 (MQ=255)
                                                                                                                 tattCATTTTTTTGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAaataata                                    <  2:718857/150‑1 (MQ=255)
                                                                                                                   ttCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAaataataat                                  >  2:1076797/1‑150 (MQ=255)
                                                                                                                    tCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATc                                 >  1:2042932/1‑150 (MQ=255)
                                                                                                                      aTTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCaa                               >  2:766303/1‑150 (MQ=255)
                                                                                                                         tttttGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAAtt                            >  1:2099107/1‑150 (MQ=255)
                                                                                                                         tttttGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAAtt                            >  1:4927735/1‑150 (MQ=255)
                                                                                                                         tttttGTCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGAGACGTTTCTTTATGATAAATAATAATCAAAtt                            >  2:4255152/1‑150 (MQ=255)
                                                                                                                           tttGTACATTGGATGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCATTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAAttta                          <  1:3202288/150‑3 (MQ=255)
                                                                                                                             tGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATa                        <  2:1949071/150‑1 (MQ=255)
                                                                                                                               tCCATTGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATaaa                      <  2:3864218/150‑1 (MQ=255)
                                                                                                                                  aTTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATc                   >  1:1285776/1‑150 (MQ=255)
                                                                                                                                    tGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCaa                 >  2:4050541/1‑150 (MQ=255)
                                                                                                                                    tGGAGGAGATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCaa                 <  1:2935904/150‑1 (MQ=255)
                                                                                                                                     ggaggaGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCaaa                >  1:1508915/1‑150 (MQ=255)
                                                                                                                                      gaggagATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCaaaa               >  2:2247453/1‑150 (MQ=255)
                                                                                                                                      gaggagATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCaaaa               >  1:3269176/1‑150 (MQ=255)
                                                                                                                                       aggagATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAAt              <  1:3637717/150‑1 (MQ=255)
                                                                                                                                       aggagATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAAt              <  1:985054/150‑1 (MQ=255)
                                                                                                                                        ggagATTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATg             <  2:14644/150‑1 (MQ=255)
                                                                                                                                          agaTTTAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATgag           <  1:5108933/150‑1 (MQ=255)
                                                                                                                                            aattAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGaa         <  2:4650703/148‑1 (MQ=255)
                                                                                                                                            aTTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGaa         <  1:1169440/150‑1 (MQ=255)
                                                                                                                                            aTTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGaa         >  1:5017622/1‑150 (MQ=255)
                                                                                                                                              ttAATTCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGaaaa       <  1:2880414/150‑1 (MQ=255)
                                                                                                                                                aaTCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGAAAAat     >  1:3031426/1‑150 (MQ=255)
                                                                                                                                                   cccTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGAAAAATATg  >  1:2358300/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTTTTGTCCATTGGAGGAGATTTAATCCCTTGCTTTTGTAAAACAAATGACAACAACGGTTCAGTGATAATTATCACATTTCAATTGCACATTAATGGATATTCTTTAATAATCTCGCGACGTTTCTTTATGATAAATAATAATCAAATTGATAAAATCAAAATGAGAAAAATATG  >  CP009273/3837030‑3837326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑