Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,850,839 G→T 39.7% P309Q (CCG→CAG)  yidJ ← sulfatase/phosphatase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,850,8390GT39.7% 411.8 / inf 349P309Q (CCG→CAG) yidJsulfatase/phosphatase superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (97/113);  new base T (71/68);  total (168/181)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

TGGCTCTTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTGGTCA  >  CP009273/3850691‑3850988
                                                                                                                                                    |                                                                                                                                                     
 ggCTCTTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGa                                                                                                                                                     >  2:2989564/1‑150 (MQ=255)
    tctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATg                                                                                                                                                  <  1:3680812/150‑1 (MQ=255)
    tctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATg                                                                                                                                                  >  1:2210133/1‑150 (MQ=255)
       ttCACGGCAAGGATATTGTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCgg                                                                                                                                               <  2:4950229/150‑1 (MQ=255)
        tCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCggg                                                                                                                                              >  1:1032503/1‑150 (MQ=255)
         cACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTACATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGt                                                                                                                                             <  2:4234762/150‑1 (MQ=255)
          aCGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTg                                                                                                                                            >  2:5320172/1‑150 (MQ=255)
            ggCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGAt                                                                                                                                          >  1:4047470/1‑150 (MQ=255)
              cAATGATATTTTCCCCCGTCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATgt                                                                                                                                        <  1:1633569/150‑1 (MQ=255)
               aaGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATgtc                                                                                                                                       >  2:4977339/1‑150 (MQ=255)
                   atatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCa                                                                                                                                   >  1:1326034/1‑150 (MQ=255)
                   atatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCa                                                                                                                                   >  2:3655132/1‑150 (MQ=255)
                   atatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCa                                                                                                                                   <  1:825874/150‑1 (MQ=255)
                    tatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCAt                                                                                                                                  >  2:4348610/1‑150 (MQ=255)
                    tatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTAGTCAt                                                                                                                                  <  2:2156514/150‑1 (MQ=255)
                     atTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATa                                                                                                                                 <  2:3744174/150‑1 (MQ=255)
                      ttttCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATAc                                                                                                                                >  1:1694747/1‑150 (MQ=255)
                         tCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATc                                                                                                                             >  1:484561/1‑150 (MQ=255)
                         tCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATc                                                                                                                             <  2:671665/150‑1 (MQ=255)
                         tCCCACGTCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATc                                                                                                                             <  2:3517434/150‑1 (MQ=255)
                          cccccGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATcg                                                                                                                            >  1:1223969/1‑150 (MQ=255)
                            cccGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATcgcc                                                                                                                          <  2:1317408/150‑1 (MQ=255)
                                 cAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGcccc                                                                                                                     <  1:1532587/150‑1 (MQ=255)
                                 cAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGcccc                                                                                                                     <  1:3170081/150‑1 (MQ=255)
                                  aGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCt                                                                                                                    >  2:5151357/1‑150 (MQ=255)
                                  aGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCt                                                                                                                    >  1:3512900/1‑150 (MQ=255)
                                   gAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCtn                                                                                                                   <  2:1691368/150‑2 (MQ=255)
                                    aaTCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCttt                                                                                                                  <  1:4281989/150‑1 (MQ=255)
                                      tctcTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTAc                                                                                                                <  2:210179/150‑1 (MQ=255)
                                      tctcTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTAc                                                                                                                >  1:4611891/1‑150 (MQ=255)
                                       ctctGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACt                                                                                                               >  2:4471758/1‑150 (MQ=255)
                                       ctctGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACt                                                                                                               >  1:992702/1‑150 (MQ=255)
                                        tctGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTg                                                                                                              <  1:3477214/150‑1 (MQ=255)
                                        tctGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTg                                                                                                              <  2:2655268/150‑1 (MQ=255)
                                          tGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGAt                                                                                                            >  2:504344/1‑150 (MQ=255)
                                             tttttCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAg                                                                                                         <  1:1215864/150‑1 (MQ=255)
                                               tttCAAGATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCt                                                                                                       <  2:3964769/150‑1 (MQ=255)
                                                ttCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCtt                                                                                                      >  2:5109693/1‑150 (MQ=255)
                                                ttCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCtt                                                                                                      <  2:5295012/150‑1 (MQ=255)
                                                  cAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTAt                                                                                                    <  2:1740038/150‑1 (MQ=255)
                                                   aaTATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTAtg                                                                                                   <  1:4571023/150‑1 (MQ=255)
                                                   aaTATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTAtg                                                                                                   <  1:1959459/150‑1 (MQ=255)
                                                     tatCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTCtgtg                                                                                                 >  2:4566984/1‑150 (MQ=255)
                                                     tatCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTAtgtg                                                                                                 >  2:4566890/1‑150 (MQ=255)
                                                        cTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTgcgc                                                                                              >  1:3122457/1‑150 (MQ=255)
                                                         tGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGcc                                                                                             >  2:4067429/1‑150 (MQ=255)
                                                         tGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGcc                                                                                             >  1:1098738/1‑150 (MQ=255)
                                                          gCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGccc                                                                                            <  1:800202/150‑1 (MQ=255)
                                                            cAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCcat                                                                                          >  1:4095495/1‑150 (MQ=255)
                                                                gcCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCAtt                                                                                      <  2:2041513/150‑1 (MQ=255)
                                                                 ccATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCAttt                                                                                     <  2:1193601/150‑1 (MQ=255)
                                                                  catcatTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTc                                                                                    <  2:1512839/150‑1 (MQ=255)
                                                                     catTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGcc                                                                                 >  1:2029032/1‑150 (MQ=255)
                                                                      atTGTCGGCAGTAAATCGATATGACCGACTGGCTAATCGACCTTTAGCCGATCCCCTTGCGGCGAACGGATGATAAGCTGAATTCGGGTGATGTAGTAATAAATCGCCGACCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGcag                                                                                >  1:5122896/1‑148 (MQ=255)
                                                                      atTGTCGGCAGTAAATCGATATGACCGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCg                                                                                >  1:2302501/1‑150 (MQ=255)
                                                                        tGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTg                                                                              >  1:5347791/1‑150 (MQ=255)
                                                                         gTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGa                                                                             <  1:4794493/150‑1 (MQ=255)
                                                                          tCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGAt                                                                            <  1:5244760/150‑1 (MQ=255)
                                                                              cAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGa                                                                        <  1:2530342/150‑1 (MQ=255)
                                                                               aGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAg                                                                       >  1:4696558/1‑150 (MQ=255)
                                                                                gTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAgg                                                                      >  1:301543/1‑150 (MQ=255)
                                                                                  aaaTCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGAGCGGAGGta                                                                    >  1:2577522/1‑150 (MQ=255)
                                                                                   aaTCGATCTGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGtat                                                                   <  1:5082540/150‑1 (MQ=255)
                                                                                      cGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTataaa                                                                >  1:71562/1‑150 (MQ=255)
                                                                                      cGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTataaa                                                                <  1:3317185/150‑1 (MQ=255)
                                                                                       gATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGCTCATCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGAGCGCCCATCATTTCGCCGTGATCGGAGGTctcaat                                                               <  2:1810373/150‑1 (MQ=255)
                                                                                       gATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTAATGTGCGCCCATCATTACGCCGTGATCGGAGGtaaagat                                                               >  2:3060605/1‑145 (MQ=255)
                                                                                       gATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTataaat                                                               <  1:550335/150‑1 (MQ=255)
                                                                                           tgactgacTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAAcc                                                           >  1:3855158/1‑150 (MQ=255)
                                                                                                gactgGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGt                                                      >  1:5332408/1‑150 (MQ=255)
                                                                                                  ctgGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTAt                                                    <  2:121276/150‑1 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                               >  2:5410196/1‑150 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                               >  1:2319686/1‑150 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                               >  1:5634949/1‑150 (MQ=255)
                                                                                                        tATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCAc                                              <  2:224166/150‑1 (MQ=255)
                                                                                                        tATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCAc                                              >  2:3355597/1‑150 (MQ=255)
                                                                                                           cGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGtt                                           >  2:5539549/1‑150 (MQ=255)
                                                                                                              ccTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGct                                        <  1:2742134/150‑1 (MQ=255)
                                                                                                              ccTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGct                                        <  1:29097/150‑1 (MQ=255)
                                                                                                              ccTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGct                                        <  1:299042/150‑1 (MQ=255)
                                                                                                                tGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGctct                                      >  2:3690476/1‑150 (MQ=255)
                                                                                                                tGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGctct                                      <  1:3777615/150‑1 (MQ=255)
                                                                                                                 gTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTg                                     >  2:243436/1‑150 (MQ=255)
                                                                                                                     ccgcTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGt                                 >  1:3965966/1‑150 (MQ=255)
                                                                                                                      cgcTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGATGTATAAATAACCCACGTCTTTTCACGTTGATCTGGCGtt                                >  2:1871112/1‑150 (MQ=255)
                                                                                                                      cgcTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGtt                                >  2:769701/1‑150 (MQ=255)
                                                                                                                       gcTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTa                               <  1:4402141/150‑1 (MQ=255)
                                                                                                                        cTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTaa                              <  2:2528532/150‑1 (MQ=255)
                                                                                                                         tCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAg                             >  2:484205/1‑150 (MQ=255)
                                                                                                                          ccccTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAgg                            <  1:5291038/150‑1 (MQ=255)
                                                                                                                             cTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCGTACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCAt                         >  1:2073806/1‑150 (MQ=255)
                                                                                                                                 cggcgAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATtgat                     >  1:186880/1‑150 (MQ=255)
                                                                                                                                  ggcgAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATtgatg                    >  1:4873094/1‑150 (MQ=255)
                                                                                                                                      aaCGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAccc                <  2:2813519/150‑1 (MQ=255)
                                                                                                                                      aaCGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGAATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAacc                >  2:2885141/1‑150 (MQ=255)
                                                                                                                                          gatgatCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAc                  >  1:926933/1‑144 (MQ=255)
                                                                                                                                          gatgatCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAc                  <  2:926933/144‑1 (MQ=255)
                                                                                                                                          gatgatCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTcc            <  2:3248493/150‑1 (MQ=255)
                                                                                                                                                cAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTg      <  1:1370401/150‑1 (MQ=255)
                                                                                                                                                 aGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTgg     >  1:238845/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGGCTCTTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTGGTCA  >  CP009273/3850691‑3850988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑