BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,250,821	C→A	5.4%	Q99K (CAG→AAG)	yjbM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,250,821	0	C	A	5.4%	1280.4 / 30.9	334	Q99K (CAG→AAG)	yjbM	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (164/152); new base A (9/9); total (173/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 361 total aligned reads displayed.

TTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAA  >  CP009273/4250672‑4250970

ttGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGTTTTTTTAACGTTTGTTAAtgcctgcc                                                                                                                                                       >  2:3352510/1‑150 (MQ=255)

ttGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAAtgcctgcc                                                                                                                                                       >  2:1828432/1‑150 (MQ=255)

  gAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAg                                                                                                                                                     <  2:464594/150‑1 (MQ=255)

   aaCAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAgg                                                                                                                                                    >  2:2830237/1‑150 (MQ=255)

     cAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGaa                                                                                                                                                  <  2:5649258/150‑1 (MQ=255)

     cAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGaa                                                                                                                                                  <  2:4934935/150‑1 (MQ=255)

      aaTGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAAc                                                                                                                                                 >  1:3310229/1‑150 (MQ=255)

      aaTGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAAc                                                                                                                                                 >  1:2064043/1‑150 (MQ=255)

      aaTGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAAc                                                                                                                                                 >  1:4890454/1‑150 (MQ=255)

         gTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACatg                                                                                                                                              >  2:1338058/1‑150 (MQ=255)

           aCTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACatgat                                                                                                                                            >  1:3247302/1‑150 (MQ=255)

            cTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACatgatg                                                                                                                                           <  2:2316220/150‑1 (MQ=255)

             ttttGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGTATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACAtgatga                                                                                                                                          <  2:127959/150‑1 (MQ=255)

              tttGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGac                                                                                                                                         <  1:4115173/150‑1 (MQ=255)

                   tGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAg                                                                                                                                    >  2:3543676/1‑150 (MQ=255)

                       gAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTa                                                                                                                                <  1:1008468/150‑1 (MQ=255)

                        aaaCTAAGAATGACCTTCCCTCAACGGTACAGCTATTTTATGAGGAATCTAAGGATAAAGAAACTTTAGCGAATATACATTTTCAAGCAATAGGATCTGGTTTTTTAACGTTTGTTAATTCATGCCAGGAACATTATGACAACAGCTTaa                                                                                                                               >  1:1448540/1‑150 (MQ=255)

                            tAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAatt                                                                                                                           >  2:2653271/1‑150 (MQ=255)

                              aGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAattat                                                                                                                         <  2:4067559/150‑1 (MQ=255)

                               gcaTGACCTTCCCTCCACGGGTCAGCTATTTTCTGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAattatt                                                                                                                        <  1:3076532/148‑1 (MQ=255)

                               gAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAattatt                                                                                                                        >  1:5178748/1‑150 (MQ=255)

                                aaTGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTAttt                                                                                                                       >  1:5348693/1‑150 (MQ=255)

                                aaTGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTATGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAGATTAAAATTAttt                                                                                                                       >  2:3955983/1‑150 (MQ=255)

                                 aTGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTg                                                                                                                      >  1:596544/1‑150 (MQ=255)

                                   gACCTTCCCTCCACGGTTCATCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCt                                                                                                                    <  2:4802298/150‑1 (MQ=255)

                                     ccTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTc                                                                                                                  >  1:5604889/1‑150 (MQ=255)

                                       ttCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGc                                                                                                                >  1:2686077/1‑150 (MQ=255)

                                        tCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCt                                                                                                               <  2:1809750/150‑1 (MQ=255)

                                        tCCCTCCACGGTTCAGCTATTTGATTAGGAATCTAAGGTTTATGAACCTTTAGCGAATTTACATTTTCTAGCAATAGGTTCTGGTTTTTTAACGTTTGATAATGCCTGCCAGGAACATGATGACTACAGCTTAAAATTATTTGCTTCGCt                                                                                                               <  1:3855302/150‑1 (MQ=255)

                                         cccTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTg                                                                                                              >  1:4781612/1‑150 (MQ=255)

                                         cccTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTg                                                                                                              >  2:3730768/1‑150 (MQ=255)

                                         cccTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTg                                                                                                              <  2:1016613/150‑1 (MQ=255)

                                         cccTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTg                                                                                                              <  1:3790777/150‑1 (MQ=255)

                                              cACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAAt                                                                                                         <  2:3183161/150‑1 (MQ=255)

                                                cGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAAttt                                                                                                       <  1:2656149/150‑1 (MQ=255)

                                                 ggTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCATGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTc                                                                                                      >  2:2721790/1‑150 (MQ=255)

                                                 ggTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTc                                                                                                      >  1:2107146/1‑150 (MQ=255)

                                                    tCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACt                                                                                                   <  1:3503964/150‑1 (MQ=255)

                                                      aGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACttt                                                                                                 <  1:3412414/150‑1 (MQ=255)

                                                      aGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACttt                                                                                                 <  1:4989558/150‑1 (MQ=255)

                                                        cTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCa                                                                                               >  2:2769627/1‑150 (MQ=255)

                                                         tATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCat                                                                                              <  2:3859176/150‑1 (MQ=255)

                                                             ttATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTc                                                                                          <  1:2830237/150‑1 (MQ=255)

                                                              tATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCt                                                                                         <  1:4250513/150‑1 (MQ=255)

                                                               aTGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTa                                                                                        <  2:962926/150‑1 (MQ=255)

                                                                 gAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGt                                                                                      >  2:4385937/1‑150 (MQ=255)

                                                                   ggAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTATAGTGc                                                                                    >  2:4170482/1‑150 (MQ=255)

                                                                     aaTCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCt                                                                                  <  1:3646036/150‑1 (MQ=255)

                                                                     aaTCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCt                                                                                  <  2:4136401/150‑1 (MQ=255)

                                                                       tCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCtcc                                                                                >  1:4964662/1‑148 (MQ=255)

                                                                       tCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTAc                                                                                <  2:1916668/150‑1 (MQ=255)

                                                                            ggATAATGAACCTTTAGCGAAGATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGa                                                                           <  2:5252125/150‑1 (MQ=255)

                                                                             gATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGAt                                                                          >  2:4238290/1‑150 (MQ=255)

                                                                              aTAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGAtt                                                                         <  1:1338058/150‑1 (MQ=255)

                                                                                 attaaCCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTAt                                                                      <  2:3224928/147‑1 (MQ=255)

                                                                                 aTGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTAt                                                                      <  1:3978300/150‑1 (MQ=255)

                                                                                    aaCCTTTAGCGAATATTCATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAg                                                                   <  1:2449684/150‑1 (MQ=255)

                                                                                    aaCCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAg                                                                   >  2:1504063/1‑150 (MQ=255)

                                                                                         ttAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACa                                                              <  1:421209/150‑1 (MQ=255)

                                                                                         ttAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACa                                                              <  2:1532257/150‑1 (MQ=255)

                                                                                         ttAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACa                                                              <  2:5048293/150‑1 (MQ=255)

                                                                                            gCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAgtg                                                           <  1:70696/150‑1 (MQ=255)

                                                                                             cGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAgtgt                                                          <  1:5630392/150‑1 (MQ=255)

                                                                                                atatACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGtata                                                       <  1:2302483/150‑1 (MQ=255)

                                                                                                atatACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGtata                                                       >  2:2796137/1‑150 (MQ=255)

                                                                                                atatACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGtata                                                       <  1:3469041/150‑1 (MQ=255)

                                                                                                  ataCATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATAtt                                                     >  2:3783902/1‑150 (MQ=255)

                                                                                                    aCATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTaa                                                   >  2:58947/1‑150 (MQ=255)

                                                                                                     caatttCAATCAATATGTTCTGGTTTTTTAACGTTTGTTAAGGCCTGCCATGAACATTATGACAACAGCTTAAAATTATTTGCTTCGCTGTTACTGTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAAt                                                  <  2:2932826/147‑1 (MQ=255)

                                                                                                      aTTTTCAAGCAATAGGTTCTGGTTTTTTATCGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATg                                                 >  2:1606055/1‑150 (MQ=255)

                                                                                                         ttCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGaaa                                              >  2:3580453/1‑150 (MQ=255)

                                                                                                         ttCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGaaa                                              >  1:847302/1‑150 (MQ=255)

                                                                                                            aaGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAaata                                           <  1:1077583/150‑1 (MQ=255)

                                                                                                                 aTAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATgag                                      <  1:5526602/150‑1 (MQ=255)

                                                                                                                  tAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATgaga                                     >  1:4656543/1‑150 (MQ=255)

                                                                                                                    ggTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGc                                   >  2:5310601/1‑150 (MQ=255)

                                                                                                                    ggTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGc                                   >  2:1380180/1‑150 (MQ=255)

                                                                                                                        cTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAACATAATGAGAGCTAcc                               >  2:3431132/1‑150 (MQ=255)

                                                                                                                        cTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTAcc                               >  2:1994419/1‑150 (MQ=255)

                                                                                                                        cTGGTTTTTTAACGTTTGTTAATGCCTGCAAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTAcc                               >  2:1161569/1‑150 (MQ=255)

                                                                                                                         tGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCt                              >  2:5519475/1‑150 (MQ=255)

                                                                                                                         tGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCt                              >  1:1105994/1‑150 (MQ=255)

                                                                                                                          ggTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTg                             >  2:167845/1‑150 (MQ=255)

                                                                                                                           gTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGa                            <  2:2477582/150‑1 (MQ=255)

                                                                                                                              ttttAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAg                         >  1:4432675/1‑150 (MQ=255)

                                                                                                                                  aaCGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCa                     <  2:3907566/150‑1 (MQ=255)

                                                                                                                                    cGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGt                   <  1:1774488/150‑1 (MQ=255)

                                                                                                                                    cGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGt                   >  2:752791/1‑150 (MQ=255)

                                                                                                                                      ttttttAATGCCTTCCAGGAAAATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGttt                 <  2:3860419/150‑1 (MQ=255)

                                                                                                                                      tttGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGttt                 >  1:2563947/1‑150 (MQ=255)

                                                                                                                                         gTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGaa              >  1:5075742/1‑150 (MQ=255)

                                                                                                                                            aaTGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGaaaaa           >  2:69390/1‑150 (MQ=255)

                                                                                                                                              tgcctgccAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAAtt         <  1:5234832/150‑1 (MQ=255)

                                                                                                                                              tgcctgccAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAAtt         >  1:1878732/1‑150 (MQ=255)

                                                                                                                                              tgcctgcaAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAAtt         >  1:549779/1‑150 (MQ=255)

                                                                                                                                               gcctgccAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTa        >  2:452950/1‑150 (MQ=255)

                                                                                                                                                cctgccAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTAt       >  1:4459470/1‑150 (MQ=255)

                                                                                                                                                 ctgcaAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTAtc      <  2:2175107/150‑1 (MQ=255)

                                                                                                                                                  tgccAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTAtct     <  1:1023715/150‑1 (MQ=255)

                                                                                                                                                    ccAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCa   <  1:1518571/150‑1 (MQ=255)

TTGAACAATGTACTTTTGATGTAGAAACTAAGAATGACCTTCCCTCCACGGTTCAGCTATTTTATGAGGAATCTAAGGATAATGAACCTTTAGCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAA  >  CP009273/4250672‑4250970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`