Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,312,246 C→A 36.3% intergenic (‑7/+14) phnF ← / ← phnE putative DNA‑binding transcriptional regulator of phosphonate uptake and biodegradation/defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,312,2460CA36.3% 454.4 / inf 317intergenic (‑7/+14)phnF/phnEputative DNA‑binding transcriptional regulator of phosphonate uptake and biodegradation/defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (81/121);  new base A (58/57);  total (139/178)
Fisher's exact test for biased strand distribution p-value = 7.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 324 total aligned reads displayed.

TTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCC  >  CP009273/4312097‑4312393
                                                                                                                                                     |                                                                                                                                                   
  cACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATaaa                                                                                                                                                   >  2:5570381/1‑150 (MQ=255)
  cACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATaaa                                                                                                                                                   >  2:2807771/1‑150 (MQ=255)
  cACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGGTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATaaa                                                                                                                                                   >  2:1248318/1‑150 (MQ=255)
   aCCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAAt                                                                                                                                                  <  1:1503898/150‑1 (MQ=255)
      tCAAAGCGCGCTGCCATTTGATGCTCTGCGTGAAGATAGACGCAGCAGAGGTAGTTTTGAAGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGAGGGTCGGTTGTGTATACAAGTGCATATATATAAATTcc                                                                                                                                               >  2:2776031/1‑150 (MQ=255)
      tCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTAGGATGTGTAGACAATTGCATAGATATCAATGcc                                                                                                                                               >  2:4739601/1‑150 (MQ=255)
      tCAAAGCGCGCTGCCAGGTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGcc                                                                                                                                               <  1:2622847/150‑1 (MQ=255)
         aaGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCg                                                                                                                                            <  1:1308724/150‑1 (MQ=255)
         aaGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCg                                                                                                                                            <  2:725899/150‑1 (MQ=255)
            cgcgcTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCtt                                                                                                                                         <  2:4879097/150‑1 (MQ=255)
             gcgcTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGGAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTa                                                                                                                                        <  1:862394/150‑1 (MQ=255)
              cgcTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTAt                                                                                                                                       <  1:2528305/150‑1 (MQ=255)
              cgcTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTAt                                                                                                                                       <  2:2485604/150‑1 (MQ=255)
              cgcTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTAt                                                                                                                                       <  1:1015102/150‑1 (MQ=255)
               gcTGACAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATc                                                                                                                                      <  2:4346899/150‑1 (MQ=255)
                cTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCa                                                                                                                                     <  2:3750366/150‑1 (MQ=255)
                 tGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAg                                                                                                                                    <  2:4315945/150‑1 (MQ=255)
                 tGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAg                                                                                                                                    >  2:4953616/1‑150 (MQ=255)
                 tGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAg                                                                                                                                    >  1:3380554/1‑150 (MQ=255)
                   ccAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGAt                                                                                                                                  >  1:582607/1‑150 (MQ=255)
                    cAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATa                                                                                                                                 >  2:3402652/1‑150 (MQ=255)
                     aGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATaa                                                                                                                                <  1:2700562/150‑1 (MQ=255)
                       ttGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAg                                                                                                                              <  2:2473299/150‑1 (MQ=255)
                        tgctgcTCGGCGGGAAGTTAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGt                                                                                                                             <  2:3942508/150‑1 (MQ=255)
                         gctgctCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTg                                                                                                                            >  2:5153875/1‑150 (MQ=255)
                                ggcggGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGc                                                                                                                     <  1:152451/150‑1 (MQ=255)
                                ggcggGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGc                                                                                                                     >  1:528413/1‑150 (MQ=255)
                                    ggAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAAcc                                                                                                                 <  2:602448/150‑1 (MQ=255)
                                     gAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCg                                                                                                                >  2:5271190/1‑150 (MQ=255)
                                     gAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCg                                                                                                                <  1:4724246/150‑1 (MQ=255)
                                       aGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGtt                                                                                                              <  2:2899054/150‑1 (MQ=255)
                                        gATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTg                                                                                                             <  2:4504731/150‑1 (MQ=255)
                                        gATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTg                                                                                                             >  1:519915/1‑150 (MQ=255)
                                                   cAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAAt                                                                                                  >  2:913333/1‑150 (MQ=255)
                                                   cAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAAt                                                                                                  <  1:295727/150‑1 (MQ=255)
                                                    aGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATc                                                                                                 >  2:154153/1‑150 (MQ=255)
                                                      cGGTAGTGTTTACGAAGCTACTGATCTAGTTTTGAGGCTATCTCTTGATAGCGTGTTGGGTAGCTGTTCGGATGTGTTGACAAGTGCATAGATATAAATGCCTCGCTTATCAGTTAAAGTGCTTACGCTACCGTTGAGAGAGGAAAtgga                                                                                               <  2:2262897/150‑4 (MQ=255)
                                                      cGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCca                                                                                               <  2:2507803/150‑1 (MQ=255)
                                                          aGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCcagcag                                                                                           >  2:4073402/1‑150 (MQ=255)
                                                           gtgtTGACGTAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGTTAGCGTGTTGGGTAGCTGGTCGGATGTGTTGACAAGTGCATAGATATCAATGCCTCGCTTATCTGATATAGTGCTTACGCAACCGTTGAGTGAGGATATCCAGCAgg                                                                                          <  2:1117965/150‑1 (MQ=255)
                                                           gtgtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAgg                                                                                          >  1:5496760/1‑150 (MQ=255)
                                                            tgtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGc                                                                                         <  2:1664004/150‑1 (MQ=255)
                                                             gtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCt                                                                                        >  1:2122322/1‑150 (MQ=255)
                                                             gtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCt                                                                                        <  1:3203823/150‑1 (MQ=255)
                                                              ttGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTg                                                                                       <  1:3809076/150‑1 (MQ=255)
                                                              ttGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTg                                                                                       >  1:4569497/1‑150 (MQ=255)
                                                              ttGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTg                                                                                       <  2:2199920/150‑1 (MQ=255)
                                                              ttGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGACATCCAGCAGGCTg                                                                                       >  2:2309878/1‑150 (MQ=255)
                                                                 aCGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGAcc                                                                                    <  1:2845392/150‑1 (MQ=255)
                                                                  cGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCg                                                                                   <  1:2800899/150‑1 (MQ=255)
                                                                     aGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGa                                                                                <  1:4115022/150‑1 (MQ=255)
                                                                       cTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACg                                                                              >  1:4989639/1‑150 (MQ=255)
                                                                       cTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACg                                                                              >  1:785797/1‑150 (MQ=255)
                                                                          cTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACgatg                                                                           >  2:3579096/1‑150 (MQ=255)
                                                                           tGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACgatga                                                                          <  2:1623184/150‑1 (MQ=255)
                                                                           tGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACgatga                                                                          <  1:5076854/150‑1 (MQ=255)
                                                                            gCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACgatgat                                                                         <  1:3927439/150‑1 (MQ=255)
                                                                               cAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAg                                                                      >  1:2209647/1‑150 (MQ=255)
                                                                                 aGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCa                                                                    <  2:541348/150‑1 (MQ=255)
                                                                                 aGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCa                                                                    <  1:1994189/150‑1 (MQ=255)
                                                                                 aGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCa                                                                    <  2:4652209/150‑1 (MQ=255)
                                                                                  gTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTTCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCAAGAAGGCTGACCGTGACGATGATAATCAc                                                                   >  2:1759215/1‑150 (MQ=255)
                                                                                       gCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCa                                                              >  1:1458502/1‑150 (MQ=255)
                                                                                        cGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAg                                                             >  1:889759/1‑150 (MQ=255)
                                                                                           cTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGgc                                                          <  1:2935810/150‑1 (MQ=255)
                                                                                            tATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGgcg                                                         <  1:279099/150‑1 (MQ=255)
                                                                                                 ctTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGt                                                    >  1:1914571/1‑150 (MQ=255)
                                                                                                 ctTGATAGAGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGt                                                    >  2:5593626/1‑150 (MQ=255)
                                                                                                  ttGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGtt                                                   >  1:2676041/1‑150 (MQ=255)
                                                                                                    gATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGGttg                                                 >  2:5182897/1‑150 (MQ=255)
                                                                                                      tAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTttgtt                                               <  2:3977567/150‑1 (MQ=255)
                                                                                                         cGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGa                                            <  2:2097213/150‑1 (MQ=255)
                                                                                                            gtTGGGTAGCTTGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACt                                         <  2:3148116/150‑1 (MQ=255)
                                                                                                            gtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACt                                         <  1:3485361/150‑1 (MQ=255)
                                                                                                              tGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTgg                                       <  2:4176037/150‑1 (MQ=255)
                                                                                                                ggTAGCTGGTCGGATGTGTAGACAAGTTCATAGCTATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGaa                                     <  1:29284/150‑1 (MQ=255)
                                                                                                                   aGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAAcc                                  <  1:3577037/150‑1 (MQ=255)
                                                                                                                   aGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAAcc                                  >  2:2628511/1‑150 (MQ=255)
                                                                                                                   aGCTGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAAcc                                  >  2:5435701/1‑150 (MQ=255)
                                                                                                                      tGGTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACcgcg                               >  2:2545841/1‑150 (MQ=255)
                                                                                                                       ggTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGa                              >  1:363869/1‑150 (MQ=255)
                                                                                                                       ggTCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGa                              >  2:1960228/1‑150 (MQ=255)
                                                                                                                        gTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTGTGTTGGAACTGGAAACCGCGaa                             >  2:789336/1‑150 (MQ=255)
                                                                                                                         tCGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAAt                            >  2:3599871/1‑150 (MQ=255)
                                                                                                                          cGGATGTGTAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATc                           >  2:5333913/1‑150 (MQ=255)
                                                                                                                           ggATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCg                          <  2:2747146/150‑1 (MQ=255)
                                                                                                                                tgtAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGCAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTcc                     <  2:1575065/150‑1 (MQ=255)
                                                                                                                                tgtAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTcc                     >  2:3832531/1‑150 (MQ=255)
                                                                                                                                 gtAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTccc                    <  2:4272676/150‑1 (MQ=255)
                                                                                                                                 gtAGACAAGTGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTccc                    <  2:1435539/150‑1 (MQ=255)
                                                                                                                                    gACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCcaca                 >  1:5057718/1‑150 (MQ=255)
                                                                                                                                       aaGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAggg              >  1:4358300/1‑150 (MQ=255)
                                                                                                                                          tGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGa           <  1:5271190/150‑1 (MQ=255)
                                                                                                                                          tGCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGa           >  1:665237/1‑150 (MQ=255)
                                                                                                                                           gCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGAc          <  1:4429731/150‑1 (MQ=255)
                                                                                                                                           gCATAGATATAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGAc          <  2:916930/150‑1 (MQ=255)
                                                                                                                                               aGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCg      >  2:2396159/1‑150 (MQ=255)
                                                                                                                                               aGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCg      >  1:2282793/1‑150 (MQ=255)
                                                                                                                                                gATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGa     >  1:4264056/1‑150 (MQ=255)
                                                                                                                                                 atatAAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGAt    >  1:4601325/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCACCTCAAAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCC  >  CP009273/4312097‑4312393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑